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Protein

Lariat debranching enzyme

Gene

Dbr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis (By similarity).By similarity1 Publication

Cofactori

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA lariat debranching enzyme activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processmRNA processing
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lariat debranching enzyme (EC:3.1.-.-)
Gene namesi
Name:Dbr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1931520 Dbr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002503591 – 550Lariat debranching enzymeAdd BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei470PhosphoserineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei485PhosphoserineBy similarity1
Modified residuei489PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1
Modified residuei494PhosphoserineCombined sources1
Modified residuei505PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ923B1
MaxQBiQ923B1
PaxDbiQ923B1
PeptideAtlasiQ923B1
PRIDEiQ923B1

PTM databases

iPTMnetiQ923B1
PhosphoSitePlusiQ923B1
SwissPalmiQ923B1

Expressioni

Gene expression databases

BgeeiENSMUSG00000032469 Expressed in 284 organ(s), highest expression level in central nervous system
CleanExiMM_DBR1
ExpressionAtlasiQ923B1 baseline and differential
GenevisibleiQ923B1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070991

Structurei

3D structure databases

ProteinModelPortaliQ923B1
SMRiQ923B1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lariat debranching enzyme family.Curated

Phylogenomic databases

eggNOGiKOG2863 Eukaryota
ENOG410XP0F LUCA
GeneTreeiENSGT00510000047481
HOGENOMiHOG000216468
HOVERGENiHBG079917
InParanoidiQ923B1
KOiK18328
OMAiRINPQTT
OrthoDBiEOG091G0AE9
PhylomeDBiQ923B1
TreeFamiTF313221

Family and domain databases

InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR007708 DBR1_C
PfamiView protein in Pfam
PF05011 DBR1, 1 hit
PF00149 Metallophos, 1 hit
SMARTiView protein in SMART
SM01124 DBR1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q923B1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVAVAGCCH GELDKIYETL ALAERRGSGP VDLLLCCGDF QAVRNEADLR
60 70 80 90 100
CMAVPPKYRH MQTFYRYYSG EKKAPVLTIF IGGNHEASNH LQELPYGGWV
110 120 130 140 150
APNIYYLGLA GVVKYRGVRI GGISGIFKSH DYRKGHFECP PYNSSTIRSI
160 170 180 190 200
YHVRNIEVYK LKQLKQPVHI FLSHDWPRNI YHYGNKKQLL KTKSFFRQEV
210 220 230 240 250
ENSTLGSPAA SELLEHLQPA YWFSAHLHVK FAALMQHQAT DKDQAGKETK
260 270 280 290 300
FLALDKCLPH RDFLQVLEIE HDPSAPEYLE YDVEWLTVLR ATDDLINVTG
310 320 330 340 350
GLWNMPEDNG LHTRWDYSAT EETMKEVMEK LNHDPKVPCN FTMTAACYDP
360 370 380 390 400
SKPQTQVKLV HRINPQTTEF CAQLGITDIN VMIQKAREEE HHQCGEYEQQ
410 420 430 440 450
GDPGTEESEE DRSEYNTDTS ALSSINPDEI MLDEEEEEEE EEEEAVSAHS
460 470 480 490 500
DMNTPSVEPA SDQASDLSTS FSDIRNLPSS MFVSSDDASR SPASGEGKCG
510 520 530 540 550
ETVESGDEKD LAKFPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDGDASAE
Length:550
Mass (Da):62,289
Last modified:September 19, 2006 - v2
Checksum:i3496D481AB876545
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YXA2D3YXA2_MOUSE
Lariat debranching enzyme
Dbr1
231Annotation score:
F6SDN8F6SDN8_MOUSE
Lariat debranching enzyme
Dbr1
83Annotation score:
F6ZYX6F6ZYX6_MOUSE
Lariat debranching enzyme
Dbr1
151Annotation score:
F6S2R9F6S2R9_MOUSE
Lariat debranching enzyme
Dbr1
164Annotation score:
F6UCV1F6UCV1_MOUSE
Lariat debranching enzyme
Dbr1
148Annotation score:

Sequence cautioni

The sequence AAK18789 differs from that shown. Reason: Frameshift at position 502.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48 – 49DL → EV in AAK18789 (PubMed:11355701).Curated2
Sequence conflicti243 – 244DQ → GR in AAH06661 (PubMed:15489334).Curated2
Sequence conflicti270E → G in BAC41182 (PubMed:16141072).Curated1
Sequence conflicti434 – 436Missing in AAH06661 (PubMed:15489334).Curated3
Sequence conflicti474I → V in BAC34064 (PubMed:16141072).Curated1
Sequence conflicti517K → N in AAK18789 (PubMed:11355701).Curated1
Sequence conflicti523H → P in AAK18789 (PubMed:11355701).Curated1
Sequence conflicti525P → R in BAC41182 (PubMed:16141072).Curated1
Sequence conflicti536Q → P in AAK18789 (PubMed:11355701).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300293 mRNA Translation: AAK18789.1 Frameshift.
AK050090 mRNA Translation: BAC34064.1
AK090351 mRNA Translation: BAC41182.1
BC006661 mRNA Translation: AAH06661.1
CCDSiCCDS23435.1
RefSeqiNP_113580.2, NM_031403.3
UniGeneiMm.31324

Genome annotation databases

EnsembliENSMUST00000066650; ENSMUSP00000070991; ENSMUSG00000032469
GeneIDi83703
KEGGimmu:83703
UCSCiuc009rei.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300293 mRNA Translation: AAK18789.1 Frameshift.
AK050090 mRNA Translation: BAC34064.1
AK090351 mRNA Translation: BAC41182.1
BC006661 mRNA Translation: AAH06661.1
CCDSiCCDS23435.1
RefSeqiNP_113580.2, NM_031403.3
UniGeneiMm.31324

3D structure databases

ProteinModelPortaliQ923B1
SMRiQ923B1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070991

PTM databases

iPTMnetiQ923B1
PhosphoSitePlusiQ923B1
SwissPalmiQ923B1

Proteomic databases

EPDiQ923B1
MaxQBiQ923B1
PaxDbiQ923B1
PeptideAtlasiQ923B1
PRIDEiQ923B1

Protocols and materials databases

DNASUi83703
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066650; ENSMUSP00000070991; ENSMUSG00000032469
GeneIDi83703
KEGGimmu:83703
UCSCiuc009rei.1 mouse

Organism-specific databases

CTDi51163
MGIiMGI:1931520 Dbr1

Phylogenomic databases

eggNOGiKOG2863 Eukaryota
ENOG410XP0F LUCA
GeneTreeiENSGT00510000047481
HOGENOMiHOG000216468
HOVERGENiHBG079917
InParanoidiQ923B1
KOiK18328
OMAiRINPQTT
OrthoDBiEOG091G0AE9
PhylomeDBiQ923B1
TreeFamiTF313221

Miscellaneous databases

PROiPR:Q923B1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032469 Expressed in 284 organ(s), highest expression level in central nervous system
CleanExiMM_DBR1
ExpressionAtlasiQ923B1 baseline and differential
GenevisibleiQ923B1 MM

Family and domain databases

InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR007708 DBR1_C
PfamiView protein in Pfam
PF05011 DBR1, 1 hit
PF00149 Metallophos, 1 hit
SMARTiView protein in SMART
SM01124 DBR1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDBR1_MOUSE
AccessioniPrimary (citable) accession number: Q923B1
Secondary accession number(s): Q8C1T9, Q8C7J7, Q99MT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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