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Protein

Vacuolar protein sorting-associated protein 5

Gene

VPS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation.

Miscellaneous

Present with 6120 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei320Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei346Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei360Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • endocytosis Source: GO_Central
  • protein retention in Golgi apparatus Source: SGD
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to Golgi Source: SGD
  • vesicle organization Source: GO_Central

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33608-MONOMER

Protein family/group databases

TCDBi9.A.63.1.1 the retromer-dependent vacuolar protein sorting (r-vps) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 5
Alternative name(s):
Carboxypeptidase Y-deficient protein 10
Vacuolar protein-targeting protein 5
Gene namesi
Name:VPS5
Synonyms:GRD2, PEP10, VPT5
Ordered Locus Names:YOR069W
ORF Names:YOR29-20
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR069W
SGDiS000005595 VPS5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138041 – 675Vacuolar protein sorting-associated protein 5Add BLAST675

Post-translational modificationi

Phosphorylated on serine residue(s).

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ92331
PaxDbiQ92331
PRIDEiQ92331

PTM databases

CarbonylDBiQ92331
iPTMnetiQ92331

Interactioni

Subunit structurei

Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPSD5 and VPS17.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi34468, 356 interactors
ComplexPortaliCPX-1653 Retromer complex
DIPiDIP-1740N
IntActiQ92331, 43 interactors
MINTiQ92331
STRINGi4932.YOR069W

Structurei

3D structure databases

ProteinModelPortaliQ92331
SMRiQ92331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini279 – 394PXPROSITE-ProRule annotationAdd BLAST116

Domaini

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization.By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144062
HOGENOMiHOG000246487
InParanoidiQ92331
KOiK17917
OMAiKAIFNQR
OrthoDBiEOG092C41VU

Family and domain databases

CDDicd07627 BAR_Vps5p, 1 hit
cd06861 PX_Vps5p, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR035803 BAR_Vps5
IPR001683 Phox
IPR036871 PX_dom_sf
IPR037868 PX_Vps5
IPR039358 Sorting_nexin-like
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Q92331-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYEDNLEAP VWDELNHEGD KTQSLVSNSI ESIGQISTNE ERKDNELLET
60 70 80 90 100
TASFADKIDL DSAPEWKDPG LSVAGNPQLE EHDNSKADDL INSLAPEQDP
110 120 130 140 150
IADLKNSTTQ FIATRESGGA LFTGNANSPL VFDDTIYDAN TSPNTSKSIS
160 170 180 190 200
GRRSGKPRIL FDSARAQRNS KRNHSLKAKR TTASDDTIKT PFTDPLKKAE
210 220 230 240 250
KENEFVEEPL DDRNERRENN EGKFTASVEK NILEQVDRPL YNLPQTGANI
260 270 280 290 300
SSPAEVEENS EKFGKTKIGS KVPPTEKAVA FKVEVKDPVK VGELTSIHVE
310 320 330 340 350
YTVISESSLL ELKYAQVSRR YRDFRWLYRQ LQNNHWGKVI PPPPEKQSVG
360 370 380 390 400
SFKENFIENR RFQMESMLKK ICQDPVLQKD KDFLLFLTSD DFSSESKKRA
410 420 430 440 450
FLTGSGAIND SNDLSEVRIS EIQLLGAEDA AEVLKNGGID AESHKGFMSI
460 470 480 490 500
SFSSLPKYNE ADEFFIEKKQ KIDELEDNLK KLSKSLEMVD TSRNTLAAST
510 520 530 540 550
EEFSSMVETL ASLNVSEPNS ELLNNFADVH KSIKSSLERS SLQETLTMGV
560 570 580 590 600
MLDDYIRSLA SVKAIFNQRS KLGYFLVVIE NDMNKKHSQL GKLGQNIHSE
610 620 630 640 650
KFREMRKEFQ TLERRYNLTK KQWQAVGDKI KDEFQGFSTD KIREFRNGME
660 670
ISLEAAIESQ KECIELWETF YQTNL
Length:675
Mass (Da):76,484
Last modified:February 1, 1997 - v1
Checksum:iFBBA994EADBDC2BD
GO

Sequence cautioni

The sequence CAA94554 differs from that shown. Reason: Frameshift at position 13.Curated
The sequence CAA99262 differs from that shown. Reason: Erroneous initiation.Curated
The sequence Z74976 differs from that shown. Reason: Frameshift at position 13.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti376V → A in AAS56361 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73512 Genomic DNA Translation: AAB62976.1
U84735 Genomic DNA Translation: AAB41798.1
Z70678 Genomic DNA Translation: CAA94554.1 Frameshift.
Z74976 Genomic DNA No translation available.
Z74977 Genomic DNA Translation: CAA99262.1 Different initiation.
AY558035 Genomic DNA Translation: AAS56361.1
BK006948 Genomic DNA Translation: DAA10847.1
PIRiS66952
RefSeqiNP_014712.2, NM_001183488.1

Genome annotation databases

EnsemblFungiiYOR069W; YOR069W; YOR069W
GeneIDi854235
KEGGisce:YOR069W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73512 Genomic DNA Translation: AAB62976.1
U84735 Genomic DNA Translation: AAB41798.1
Z70678 Genomic DNA Translation: CAA94554.1 Frameshift.
Z74976 Genomic DNA No translation available.
Z74977 Genomic DNA Translation: CAA99262.1 Different initiation.
AY558035 Genomic DNA Translation: AAS56361.1
BK006948 Genomic DNA Translation: DAA10847.1
PIRiS66952
RefSeqiNP_014712.2, NM_001183488.1

3D structure databases

ProteinModelPortaliQ92331
SMRiQ92331
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34468, 356 interactors
ComplexPortaliCPX-1653 Retromer complex
DIPiDIP-1740N
IntActiQ92331, 43 interactors
MINTiQ92331
STRINGi4932.YOR069W

Protein family/group databases

TCDBi9.A.63.1.1 the retromer-dependent vacuolar protein sorting (r-vps) family

PTM databases

CarbonylDBiQ92331
iPTMnetiQ92331

Proteomic databases

MaxQBiQ92331
PaxDbiQ92331
PRIDEiQ92331

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR069W; YOR069W; YOR069W
GeneIDi854235
KEGGisce:YOR069W

Organism-specific databases

EuPathDBiFungiDB:YOR069W
SGDiS000005595 VPS5

Phylogenomic databases

GeneTreeiENSGT00910000144062
HOGENOMiHOG000246487
InParanoidiQ92331
KOiK17917
OMAiKAIFNQR
OrthoDBiEOG092C41VU

Enzyme and pathway databases

BioCyciYEAST:G3O-33608-MONOMER

Miscellaneous databases

PROiPR:Q92331

Family and domain databases

CDDicd07627 BAR_Vps5p, 1 hit
cd06861 PX_Vps5p, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR035803 BAR_Vps5
IPR001683 Phox
IPR036871 PX_dom_sf
IPR037868 PX_Vps5
IPR039358 Sorting_nexin-like
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPS5_YEAST
AccessioniPrimary (citable) accession number: Q92331
Secondary accession number(s): D6W2D1, E9P8U6, Q08483
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 12, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
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