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Protein

Pyrroline-5-carboxylate reductase 2

Gene

Pycr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP+. Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).By similarity

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.By similarity

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase 2 (Pycr2), Pyrroline-5-carboxylate reductase 1, mitochondrial (Pycr1), Pyrroline-5-carboxylate reductase 3 (Pycr3), Pyrroline-5-carboxylate reductase (Pycr2), Pyrroline-5-carboxylate reductase (Pycr1), Pyrroline-5-carboxylate reductase (Pycr2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADP; via carbonyl oxygenBy similarity1
Binding sitei56NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 11NADPBy similarity6
Nucleotide bindingi69 – 72NADPBy similarity4
Nucleotide bindingi95 – 97NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayi
UPA00098;UER00361

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 2 (EC:1.5.1.2)
Short name:
P5C reductase 2
Short name:
P5CR 2
Gene namesi
Name:Pycr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1277956 Pycr2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001873182 – 320Pyrroline-5-carboxylate reductase 2Add BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei304PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922Q4
MaxQBiQ922Q4
PaxDbiQ922Q4
PRIDEiQ922Q4

PTM databases

iPTMnetiQ922Q4
PhosphoSitePlusiQ922Q4
SwissPalmiQ922Q4

Expressioni

Gene expression databases

BgeeiENSMUSG00000026520 Expressed in 298 organ(s), highest expression level in dorsal pancreas
ExpressionAtlasiQ922Q4 baseline and differential
GenevisibleiQ922Q4 MM

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers. Interacts with LTO1.By similarity

Protein-protein interaction databases

BioGridi213197, 2 interactors
IntActiQ922Q4, 2 interactors
MINTiQ922Q4
STRINGi10090.ENSMUSP00000027802

Structurei

3D structure databases

ProteinModelPortaliQ922Q4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3124 Eukaryota
COG0345 LUCA
GeneTreeiENSGT00390000007443
HOGENOMiHOG000230247
HOVERGENiHBG053399
InParanoidiQ922Q4
KOiK00286
OMAiEQICTPK
OrthoDBiEOG091G0KMI
PhylomeDBiQ922Q4

Family and domain databases

HAMAPiMF_01925 P5C_reductase, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR036291 NAD(P)-bd_dom_sf
IPR028939 P5C_Rdtase_cat_N
IPR029036 P5CR_dimer
IPR000304 Pyrroline-COOH_reductase
PfamiView protein in Pfam
PF03807 F420_oxidored, 1 hit
PF14748 P5CR_dimer, 1 hit
PIRSFiPIRSF000193 Pyrrol-5-carb_rd, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00112 proC, 1 hit
PROSITEiView protein in PROSITE
PS00521 P5CR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922Q4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVGFIGAGQ LACALARGFT AAGVLSAHKI IASSPDMDLP TVSALRRMGV
60 70 80 90 100
NLTRSNKDTV RHSDVLFLAV KPHIIPFILD EIGADVQERH IVVSCAAGVT
110 120 130 140 150
ISSVEKKLMA FQPAPKVIRC MTNTPVVVRE GATVYATGTH ALVEDGKLLE
160 170 180 190 200
QLMSSVGFCT EVEEDLIDAI TGLSGSGPAY AFMALDALAD GGVKMGVPRR
210 220 230 240 250
LAVRLGAQAL LGAAKMLLDS EDHPGQLKDN VCSPGGATIH ALHFLESGGF
260 270 280 290 300
RSLLINAVEA SCIRTRELQS MADQEKVSPA ALKKTLLDRV KLESPTVSTL
310 320
APPSSGKLLT RNPAQGSKKE
Length:320
Mass (Da):33,659
Last modified:December 1, 2001 - v1
Checksum:i64DD7F96FB8C4BC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006882 mRNA Translation: AAH06882.1
CCDSiCCDS15576.1
RefSeqiNP_598466.1, NM_133705.2
UniGeneiMm.274180

Genome annotation databases

EnsembliENSMUST00000027802; ENSMUSP00000027802; ENSMUSG00000026520
GeneIDi69051
KEGGimmu:69051
UCSCiuc007dwv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006882 mRNA Translation: AAH06882.1
CCDSiCCDS15576.1
RefSeqiNP_598466.1, NM_133705.2
UniGeneiMm.274180

3D structure databases

ProteinModelPortaliQ922Q4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213197, 2 interactors
IntActiQ922Q4, 2 interactors
MINTiQ922Q4
STRINGi10090.ENSMUSP00000027802

PTM databases

iPTMnetiQ922Q4
PhosphoSitePlusiQ922Q4
SwissPalmiQ922Q4

Proteomic databases

EPDiQ922Q4
MaxQBiQ922Q4
PaxDbiQ922Q4
PRIDEiQ922Q4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027802; ENSMUSP00000027802; ENSMUSG00000026520
GeneIDi69051
KEGGimmu:69051
UCSCiuc007dwv.2 mouse

Organism-specific databases

CTDi29920
MGIiMGI:1277956 Pycr2

Phylogenomic databases

eggNOGiKOG3124 Eukaryota
COG0345 LUCA
GeneTreeiENSGT00390000007443
HOGENOMiHOG000230247
HOVERGENiHBG053399
InParanoidiQ922Q4
KOiK00286
OMAiEQICTPK
OrthoDBiEOG091G0KMI
PhylomeDBiQ922Q4

Enzyme and pathway databases

UniPathwayi
UPA00098;UER00361

ReactomeiR-MMU-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

ChiTaRSiPycr2 mouse
PROiPR:Q922Q4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026520 Expressed in 298 organ(s), highest expression level in dorsal pancreas
ExpressionAtlasiQ922Q4 baseline and differential
GenevisibleiQ922Q4 MM

Family and domain databases

HAMAPiMF_01925 P5C_reductase, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR036291 NAD(P)-bd_dom_sf
IPR028939 P5C_Rdtase_cat_N
IPR029036 P5CR_dimer
IPR000304 Pyrroline-COOH_reductase
PfamiView protein in Pfam
PF03807 F420_oxidored, 1 hit
PF14748 P5CR_dimer, 1 hit
PIRSFiPIRSF000193 Pyrrol-5-carb_rd, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00112 proC, 1 hit
PROSITEiView protein in PROSITE
PS00521 P5CR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP5CR2_MOUSE
AccessioniPrimary (citable) accession number: Q922Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: October 10, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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