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Protein

Negative elongation factor D

Gene

Nelfcd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor D
Short name:
NELF-D
Alternative name(s):
TH1-like protein
Gene namesi
Name:Nelfcd
Synonyms:Nelfd, Th1, Th1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926424 Nelfcd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191321 – 591Negative elongation factor DAdd BLAST591

Proteomic databases

EPDiQ922L6
MaxQBiQ922L6
PaxDbiQ922L6
PRIDEiQ922L6

PTM databases

PhosphoSitePlusiQ922L6

Expressioni

Gene expression databases

BgeeiENSMUSG00000016253 Expressed in 294 organ(s), highest expression level in embryo
CleanExiMM_TH1L
ExpressionAtlasiQ922L6 baseline and differential
GenevisibleiQ922L6 MM

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE; NELFA and NELFCD form a stable subcomplex that binds primarily through NELFCD to the N-terminus of NELFB. Interacts with ARAF1. Interacts with PCF11.By similarity

Protein-protein interaction databases

BioGridi208252, 2 interactors
STRINGi10090.ENSMUSP00000104703

Structurei

3D structure databases

SMRiQ922L6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NELF-D family.Curated

Phylogenomic databases

eggNOGiENOG410IFGF Eukaryota
ENOG410XSDJ LUCA
GeneTreeiENSGT00390000001799
HOGENOMiHOG000231762
HOVERGENiHBG052597
InParanoidiQ922L6
KOiK15181

Family and domain databases

InterProiView protein in InterPro
IPR006942 TH1
PANTHERiPTHR12144 PTHR12144, 1 hit
PfamiView protein in Pfam
PF04858 TH1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q922L6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGPAPGTIM GEDYFGNASE WGEEADGGQH QEDDSGEGED DAEVQQECLH
60 70 80 90 100
KFSTRDYIME PSIFNTLKRY FQAGGSPENV IQLLSENYTA VAQTVNLLAE
110 120 130 140 150
WLIQTGVEPV QVQETVENHL KSLLIKHFDP RKADSIFTEE GETPAWLEQM
160 170 180 190 200
IAHTTWRDLF YKLAEAHPDC LMLNFTVKLI SDAGYQGEIT SVSTACQQLE
210 220 230 240 250
VFSRVLRTSL ATILDGGEEN LEKNLPEFAK MVCHGEHTYL FAQAMMSVLA
260 270 280 290 300
QEEQGGSAVR RVAQEVQRFA QEKGHDASQI TLALGTAASY PRACQALGAM
310 320 330 340 350
LSRGALNPAD ITVLFKMFTS MDPPPVELIR VPAFLDLFMQ SLFKPGAKIN
360 370 380 390 400
QDHKHKYIHI LAYAASVVET WKKNKRVSIG KDELKSTSKA IETVHNLCCN
410 420 430 440 450
ENKGASELVA ELSTLYQCIR FPVVAMGVLK WVDWTVSEPR YFQLQTDHTP
460 470 480 490 500
VHLALLDEIS TCHQLLHPQV LQLLVKLFET EHSQLDVMEQ LELKKTLLDR
510 520 530 540 550
MVHLLSRGYV LPVVSYIRKC LEKLDTDISL IRYFVTEVLD VIAPPYTSDF
560 570 580 590
VQLFLPILEN DSIAGTIKAE GEHDPVTEFI AHCKSNFIVV N
Length:591
Mass (Da):66,276
Last modified:November 14, 2003 - v2
Checksum:i33DB33547688715D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TW27Q3TW27_MOUSE
Negative elongation factor D
Nelfcd Th1l
607Annotation score:

Sequence cautioni

The sequence AAH31747 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB26880 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC33744 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB65252 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB65253 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB65254 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38G → V in AAH06959 (PubMed:15489334).Curated1
Sequence conflicti416Y → C in CAB65253 (PubMed:11030415).Curated1
Sequence conflicti479E → G in CAB65254 (PubMed:11030415).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238741 mRNA Translation: CAB65252.1 Different initiation.
AJ238742 mRNA Translation: CAB65253.1 Different initiation.
AJ238743 mRNA Translation: CAB65254.1 Different initiation.
AK010360 mRNA Translation: BAB26880.1 Different initiation.
AK049418 mRNA Translation: BAC33744.1 Different initiation.
AL844534 Genomic DNA No translation available.
BC006959 mRNA Translation: AAH06959.1
BC031747 mRNA Translation: AAH31747.1 Different initiation.
CCDSiCCDS38358.1
RefSeqiNP_065605.2, NM_020580.3
UniGeneiMm.271709

Genome annotation databases

EnsembliENSMUST00000109075; ENSMUSP00000104703; ENSMUSG00000016253
GeneIDi57314
KEGGimmu:57314
UCSCiuc008ofc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238741 mRNA Translation: CAB65252.1 Different initiation.
AJ238742 mRNA Translation: CAB65253.1 Different initiation.
AJ238743 mRNA Translation: CAB65254.1 Different initiation.
AK010360 mRNA Translation: BAB26880.1 Different initiation.
AK049418 mRNA Translation: BAC33744.1 Different initiation.
AL844534 Genomic DNA No translation available.
BC006959 mRNA Translation: AAH06959.1
BC031747 mRNA Translation: AAH31747.1 Different initiation.
CCDSiCCDS38358.1
RefSeqiNP_065605.2, NM_020580.3
UniGeneiMm.271709

3D structure databases

SMRiQ922L6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208252, 2 interactors
STRINGi10090.ENSMUSP00000104703

PTM databases

PhosphoSitePlusiQ922L6

Proteomic databases

EPDiQ922L6
MaxQBiQ922L6
PaxDbiQ922L6
PRIDEiQ922L6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109075; ENSMUSP00000104703; ENSMUSG00000016253
GeneIDi57314
KEGGimmu:57314
UCSCiuc008ofc.1 mouse

Organism-specific databases

CTDi51497
MGIiMGI:1926424 Nelfcd

Phylogenomic databases

eggNOGiENOG410IFGF Eukaryota
ENOG410XSDJ LUCA
GeneTreeiENSGT00390000001799
HOGENOMiHOG000231762
HOVERGENiHBG052597
InParanoidiQ922L6
KOiK15181

Miscellaneous databases

ChiTaRSiNelfcd mouse
PROiPR:Q922L6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016253 Expressed in 294 organ(s), highest expression level in embryo
CleanExiMM_TH1L
ExpressionAtlasiQ922L6 baseline and differential
GenevisibleiQ922L6 MM

Family and domain databases

InterProiView protein in InterPro
IPR006942 TH1
PANTHERiPTHR12144 PTHR12144, 1 hit
PfamiView protein in Pfam
PF04858 TH1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNELFD_MOUSE
AccessioniPrimary (citable) accession number: Q922L6
Secondary accession number(s): A2AQ05
, Q9QXC8, Q9QXC9, Q9QXD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: September 12, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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