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Protein

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial

Gene

Pdk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species (By similarity).By similarity

Catalytic activityi

ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 254ATPBy similarity8
Nucleotide bindingi306 – 307ATPBy similarity2
Nucleotide bindingi323 – 328ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-MMU-5362517 Signaling by Retinoic Acid

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial (EC:2.7.11.2)
Alternative name(s):
Pyruvate dehydrogenase kinase isoform 3
Gene namesi
Name:Pdk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2384308 Pdk3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000023444? – 415[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278N6-succinyllysineCombined sources1

Proteomic databases

EPDiQ922H2
MaxQBiQ922H2
PaxDbiQ922H2
PRIDEiQ922H2

PTM databases

iPTMnetiQ922H2
PhosphoSitePlusiQ922H2

Expressioni

Gene expression databases

BgeeiENSMUSG00000035232 Expressed in 303 organ(s), highest expression level in testis
CleanExiMM_PDK3
ExpressionAtlasiQ922H2 baseline and differential
GenevisibleiQ922H2 MM

Interactioni

Subunit structurei

Homodimer. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (By similarity).By similarity

Protein-protein interaction databases

BioGridi231815, 1 interactor
IntActiQ922H2, 2 interactors
STRINGi10090.ENSMUSP00000036604

Structurei

3D structure databases

ProteinModelPortaliQ922H2
SMRiQ922H2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 362Histidine kinasePROSITE-ProRule annotationAdd BLAST232

Sequence similaritiesi

Belongs to the PDK/BCKDK protein kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0787 Eukaryota
COG0642 LUCA
GeneTreeiENSGT00550000074574
HOGENOMiHOG000164315
HOVERGENiHBG000511
InParanoidiQ922H2
KOiK00898
OMAiAPNKPIQ
OrthoDBiEOG091G07DJ
PhylomeDBiQ922H2
TreeFamiTF314918

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.140.20, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR036784 AK/P_DHK_N_sf
IPR018955 BCDHK/PDK_N
IPR039028 BCKD/PDK
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
PANTHERiPTHR11947 PTHR11947, 1 hit
PfamiView protein in Pfam
PF10436 BCDHK_Adom3, 1 hit
PF02518 HATPase_c, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF55874 SSF55874, 1 hit
SSF69012 SSF69012, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922H2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLFYRLLKQ PVPKQIERYS RFSPSPLSIK QFLDFGRDNA CEKTSYMFLR
60 70 80 90 100
KELPVRLANT MREVNLLPDN LLNRPSVGLV QSWYMQSFLE LLEYENKSPE
110 120 130 140 150
DPRVLDNFLN VLINIRNRHN DVVPTMAQGV IEYKEKFGFD PFISSNIQYF
160 170 180 190 200
LDRFYTNRIS FRMLINQHTL LFGGDTNPAH PKHIGSIDPT CNVADVVKDA
210 220 230 240 250
YETAKMLCEQ YYLVAPELEV EEFNAKAPNK PIQVVYVPSH LFHMLFELFK
260 270 280 290 300
NSMRATVELH EDKKEGYPAV KTLVTLGKED LSIKISDLGG GVPLRKIDRL
310 320 330 340 350
FNYMYSTAPR PSLEPTRAAP LAGFGYGLPI SRLYARYFQG DLKLYSMEGV
360 370 380 390 400
GTDAVIYLKA LSSESFERLP VFNKSAWRHY KTTPEADDWS NPSSEPRDAS
410
KYKAKQDKIK SNRTF
Length:415
Mass (Da):47,923
Last modified:December 1, 2001 - v1
Checksum:i566497E0F8842822
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21R → C in BAC40379 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075879 mRNA Translation: BAC36024.1
AK075985 mRNA Translation: BAC36097.1
AK088478 mRNA Translation: BAC40379.1
BC008126 mRNA Translation: AAH08126.1
CCDSiCCDS30275.1
RefSeqiNP_663605.1, NM_145630.2
UniGeneiMm.12775

Genome annotation databases

EnsembliENSMUST00000045748; ENSMUSP00000036604; ENSMUSG00000035232
GeneIDi236900
KEGGimmu:236900
UCSCiuc009tsx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075879 mRNA Translation: BAC36024.1
AK075985 mRNA Translation: BAC36097.1
AK088478 mRNA Translation: BAC40379.1
BC008126 mRNA Translation: AAH08126.1
CCDSiCCDS30275.1
RefSeqiNP_663605.1, NM_145630.2
UniGeneiMm.12775

3D structure databases

ProteinModelPortaliQ922H2
SMRiQ922H2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231815, 1 interactor
IntActiQ922H2, 2 interactors
STRINGi10090.ENSMUSP00000036604

PTM databases

iPTMnetiQ922H2
PhosphoSitePlusiQ922H2

Proteomic databases

EPDiQ922H2
MaxQBiQ922H2
PaxDbiQ922H2
PRIDEiQ922H2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045748; ENSMUSP00000036604; ENSMUSG00000035232
GeneIDi236900
KEGGimmu:236900
UCSCiuc009tsx.1 mouse

Organism-specific databases

CTDi5165
MGIiMGI:2384308 Pdk3

Phylogenomic databases

eggNOGiKOG0787 Eukaryota
COG0642 LUCA
GeneTreeiENSGT00550000074574
HOGENOMiHOG000164315
HOVERGENiHBG000511
InParanoidiQ922H2
KOiK00898
OMAiAPNKPIQ
OrthoDBiEOG091G07DJ
PhylomeDBiQ922H2
TreeFamiTF314918

Enzyme and pathway databases

ReactomeiR-MMU-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-MMU-5362517 Signaling by Retinoic Acid

Miscellaneous databases

PROiPR:Q922H2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035232 Expressed in 303 organ(s), highest expression level in testis
CleanExiMM_PDK3
ExpressionAtlasiQ922H2 baseline and differential
GenevisibleiQ922H2 MM

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.140.20, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR036784 AK/P_DHK_N_sf
IPR018955 BCDHK/PDK_N
IPR039028 BCKD/PDK
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
PANTHERiPTHR11947 PTHR11947, 1 hit
PfamiView protein in Pfam
PF10436 BCDHK_Adom3, 1 hit
PF02518 HATPase_c, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF55874 SSF55874, 1 hit
SSF69012 SSF69012, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPDK3_MOUSE
AccessioniPrimary (citable) accession number: Q922H2
Secondary accession number(s): Q8BTX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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