UniProtKB - Q921T2 (TOIP1_MOUSE)
Protein
Torsin-1A-interacting protein 1
Gene
Tor1aip1
Organism
Mus musculus (Mouse)
Status
Functioni
Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.1 Publication
GO - Molecular functioni
- ATPase activator activity Source: UniProtKB
- ATPase binding Source: MGI
- cytoskeletal protein binding Source: MGI
- lamin binding Source: MGI
GO - Biological processi
- nuclear membrane organization Source: MGI
- positive regulation of ATPase activity Source: UniProtKB
- protein localization to nuclear envelope Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Torsin-1A-interacting protein 1Alternative name(s): Lamina-associated polypeptide 1B Short name: LAP1B |
Gene namesi | Name:Tor1aip1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3582693 Tor1aip1 |
Subcellular locationi
Nucleus
- Nucleus inner membrane By similarity; Single-pass membrane protein By similarity
Nucleus
- nuclear envelope Source: MGI
- nuclear inner membrane Source: UniProtKB-SubCell
- nuclear membrane Source: MGI
- nucleus Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 351 | NuclearSequence analysisAdd BLAST | 351 | |
Transmembranei | 352 – 372 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 373 – 595 | Perinuclear spaceSequence analysisAdd BLAST | 223 |
Keywords - Cellular componenti
Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutant mice exhibit perinatal mortality, typically dying on the last prenatal or first postnatal day. All tissues tested exhibit nuclear membrane abnormalities with membranous vesicle-appearing structures observed in the perinuclear space of neurons.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000228836 | 1 – 595 | Torsin-1A-interacting protein 1Add BLAST | 595 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | PhosphoserineCombined sources | 1 | |
Modified residuei | 134 | PhosphoserineBy similarity | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 151 | PhosphoserineCombined sources | 1 | |
Modified residuei | 153 | PhosphoserineBy similarity | 1 | |
Modified residuei | 154 | PhosphoserineBy similarity | 1 | |
Modified residuei | 155 | PhosphoserineBy similarity | 1 | |
Modified residuei | 235 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 241 | PhosphoserineBy similarity | 1 | |
Modified residuei | 244 | PhosphoserineBy similarity | 1 | |
Modified residuei | 255 | PhosphoserineBy similarity | 1 | |
Cross-linki | 321 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 328 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 411 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q921T2 |
PaxDbi | Q921T2 |
PeptideAtlasi | Q921T2 |
PRIDEi | Q921T2 |
PTM databases
GlyConnecti | 2772 |
iPTMneti | Q921T2 |
PhosphoSitePlusi | Q921T2 |
SwissPalmi | Q921T2 |
Expressioni
Tissue specificityi
Expressed in the spinal cord and liver (at protein level).1 Publication
Developmental stagei
At 16 dpc, widely expressed with high expression levels in hippocampus and low levels in heart. In the spinal cord, expressed as early as 12 dpc until p21, the expression levels decrease in the adulthood (at protein level).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000026466 Expressed in 266 organ(s), highest expression level in mesenteric lymph node |
ExpressionAtlasi | Q921T2 baseline and differential |
Genevisiblei | Q921T2 MM |
Interactioni
Subunit structurei
Interacts with ATP1B4.
Interacts with TOR1A (ATP-bound).
Interacts with TOR1B, TOR2A and TOR3A.
Interacts with VIM.
1 PublicationGO - Molecular functioni
- ATPase binding Source: MGI
- cytoskeletal protein binding Source: MGI
- lamin binding Source: MGI
Protein-protein interaction databases
BioGridi | 228970, 36 interactors |
DIPi | DIP-56693N |
IntActi | Q921T2, 36 interactors |
MINTi | Q921T2 |
STRINGi | 10090.ENSMUSP00000027738 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 368 – 595 | Interaction with TOR1ABy similarityAdd BLAST | 228 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 373 – 400 | Sequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 129 | Arg-richAdd BLAST | 108 |
Sequence similaritiesi
Belongs to the TOR1AIP family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410IJUB Eukaryota ENOG4111IZJ LUCA |
GeneTreei | ENSGT00390000012166 |
HOGENOMi | HOG000015293 |
InParanoidi | Q921T2 |
KOi | K23001 |
OMAi | QKNPQEW |
OrthoDBi | 575658at2759 |
TreeFami | TF329438 |
Family and domain databases
Gene3Di | 3.40.50.12190, 1 hit |
InterProi | View protein in InterPro IPR008662 Lamina-ass_polypeptide_CLAP1C IPR038599 LAP1C-like_C_sf |
PANTHERi | PTHR18843 PTHR18843, 1 hit |
Pfami | View protein in Pfam PF05609 LAP1C, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q921T2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGERWQAEG PGEGWAIYVT PRAPIREGRR RLDPRNGDSS DAPAYGAHPS
60 70 80 90 100
RRGRREVRFS EEPAEVYGDF EPRAAKERSP GGRRTPPEKF RPASAGEEVR
110 120 130 140 150
ESAYNLRSRP RRQRRAQEAE EMKTRRSARL EQHSQQPQLS PATSGRGLRD
160 170 180 190 200
SPSSSEDREE DEPSSRPVTS QTASKKTLRT PEASVMNEDP ISNLCRPPLR
210 220 230 240 250
SPRLDSTYQT NGNTKTNERE ATIVQQVNFF EEGETEDDLE SSYSDITIRA
260 270 280 290 300
RSSDSLESRD EATPAAGNHP DSLRGLPHNQ DFPAHENQPL LLTSGCQENP
310 320 330 340 350
QEWVDRAVRM RSRMAYNNIQ KSNFGNQSPS TSRPQSAIHH PNEPSVKIKW
360 370 380 390 400
WLLGLVAILA VGLFWFFHTP AVETTAVQEF QNQMKQLQSK YQSQNEKLWK
410 420 430 440 450
RGTTFLEKHL NSSLPRPQPA ILLLTAAQDA AEVLKCLSEQ IADAYSSFRS
460 470 480 490 500
VRAIRIDGAG KAAQDSDLVK HEVDQELTDG FKNGQNAAVV HRFESLPAGS
510 520 530 540 550
TLIFYKYCDH ENAAFKDVAL VLTVLLEEKT LEASLGLKEI EEKVRDFLKV
560 570 580 590
KFTSSSTASS YNHMDPDKLN GLWSRISHLV LPVQPENTLK AGSCL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PWW2 | E9PWW2_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 391 | Annotation score: | ||
D3YZJ9 | D3YZJ9_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 190 | Annotation score: | ||
D3YZP0 | D3YZP0_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 176 | Annotation score: | ||
J3QQ40 | J3QQ40_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 291 | Annotation score: | ||
Q3UE61 | Q3UE61_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 324 | Annotation score: | ||
D3YXF8 | D3YXF8_MOUSE | Torsin-1A-interacting protein 1 | Tor1aip1 | 102 | Annotation score: |
Sequence cautioni
The sequence BAE31466 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | S → A in BAE31466 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 254 | D → N in BAE94915 (Ref. 1) Curated | 1 | |
Sequence conflicti | 254 | D → N in AAH10841 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042952 | 260 – 335 | DEATP…TSRPQ → E in isoform 3. 1 PublicationAdd BLAST | 76 | |
Alternative sequenceiVSP_042953 | 261 – 279 | Missing in isoform 2. 1 PublicationAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB251963 mRNA Translation: BAE94915.1 AC159964 Genomic DNA No translation available. BC010841 mRNA Translation: AAH10841.1 AK152751 mRNA Translation: BAE31466.1 Different initiation. |
CCDSi | CCDS15387.1 [Q921T2-3] CCDS48402.1 [Q921T2-2] |
RefSeqi | NP_001153490.1, NM_001160018.1 [Q921T2-2] NP_001153491.1, NM_001160019.1 NP_659040.2, NM_144791.2 [Q921T2-3] |
Genome annotation databases
Ensembli | ENSMUST00000027738; ENSMUSP00000027738; ENSMUSG00000026466 [Q921T2-3] ENSMUST00000097527; ENSMUSP00000095134; ENSMUSG00000026466 [Q921T2-2] |
GeneIDi | 208263 |
KEGGi | mmu:208263 |
UCSCi | uc007dbu.2 mouse [Q921T2-3] uc007dbw.2 mouse [Q921T2-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB251963 mRNA Translation: BAE94915.1 AC159964 Genomic DNA No translation available. BC010841 mRNA Translation: AAH10841.1 AK152751 mRNA Translation: BAE31466.1 Different initiation. |
CCDSi | CCDS15387.1 [Q921T2-3] CCDS48402.1 [Q921T2-2] |
RefSeqi | NP_001153490.1, NM_001160018.1 [Q921T2-2] NP_001153491.1, NM_001160019.1 NP_659040.2, NM_144791.2 [Q921T2-3] |
3D structure databases
SMRi | Q921T2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 228970, 36 interactors |
DIPi | DIP-56693N |
IntActi | Q921T2, 36 interactors |
MINTi | Q921T2 |
STRINGi | 10090.ENSMUSP00000027738 |
PTM databases
GlyConnecti | 2772 |
iPTMneti | Q921T2 |
PhosphoSitePlusi | Q921T2 |
SwissPalmi | Q921T2 |
Proteomic databases
jPOSTi | Q921T2 |
PaxDbi | Q921T2 |
PeptideAtlasi | Q921T2 |
PRIDEi | Q921T2 |
Genome annotation databases
Ensembli | ENSMUST00000027738; ENSMUSP00000027738; ENSMUSG00000026466 [Q921T2-3] ENSMUST00000097527; ENSMUSP00000095134; ENSMUSG00000026466 [Q921T2-2] |
GeneIDi | 208263 |
KEGGi | mmu:208263 |
UCSCi | uc007dbu.2 mouse [Q921T2-3] uc007dbw.2 mouse [Q921T2-2] |
Organism-specific databases
CTDi | 26092 |
MGIi | MGI:3582693 Tor1aip1 |
Phylogenomic databases
eggNOGi | ENOG410IJUB Eukaryota ENOG4111IZJ LUCA |
GeneTreei | ENSGT00390000012166 |
HOGENOMi | HOG000015293 |
InParanoidi | Q921T2 |
KOi | K23001 |
OMAi | QKNPQEW |
OrthoDBi | 575658at2759 |
TreeFami | TF329438 |
Miscellaneous databases
ChiTaRSi | Tor1aip1 mouse |
PROi | PR:Q921T2 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026466 Expressed in 266 organ(s), highest expression level in mesenteric lymph node |
ExpressionAtlasi | Q921T2 baseline and differential |
Genevisiblei | Q921T2 MM |
Family and domain databases
Gene3Di | 3.40.50.12190, 1 hit |
InterProi | View protein in InterPro IPR008662 Lamina-ass_polypeptide_CLAP1C IPR038599 LAP1C-like_C_sf |
PANTHERi | PTHR18843 PTHR18843, 1 hit |
Pfami | View protein in Pfam PF05609 LAP1C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOIP1_MOUSE | |
Accessioni | Q921T2Primary (citable) accession number: Q921T2 Secondary accession number(s): E9QLK1, Q1EQW1, Q3U7A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | April 18, 2012 | |
Last modified: | October 16, 2019 | |
This is version 129 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot