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UniProtKB - Q921Q3 (ALG1_MOUSE)
Protein
Chitobiosyldiphosphodolichol beta-mannosyltransferase
Gene
Alg1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the addition of the first of nine mannose moieties to form a dolichol-lipid linked oligosaccharide intermediate required for proper N-linked glycosylation.
By similarityCatalytic activityi
- GDP-α-D-mannose + N,N'-diacetylchitobiosyl diphosphodolichol = β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + GDP + H+By similarityEC:2.4.1.142By similarityThis reaction proceeds in the forwardBy similarity direction.
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- chitobiosyldiphosphodolichol beta-mannosyltransferase activity Source: UniProtKB-EC
- mannosyltransferase activity Source: GO_Central
GO - Biological processi
- protein glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT33, Glycosyltransferase Family 33 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitobiosyldiphosphodolichol beta-mannosyltransferaseCurated (EC:2.4.1.142By similarity)Alternative name(s): Asparagine-linked glycosylation protein 1 homolog Beta-1,4-mannosyltransferase GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase GDP-mannose-dolichol diphosphochitobiose mannosyltransferase |
Gene namesi | Name:Alg1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2384774, Alg1 |
VEuPathDBi | HostDB:ENSMUSG00000039427 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 2 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 3 – 23 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 24 – 482 | LumenalSequence analysisAdd BLAST | 459 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080250 | 1 – 482 | Chitobiosyldiphosphodolichol beta-mannosyltransferaseAdd BLAST | 482 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 242 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q921Q3 |
MaxQBi | Q921Q3 |
PaxDbi | Q921Q3 |
PeptideAtlasi | Q921Q3 |
PRIDEi | Q921Q3 |
ProteomicsDBi | 296221 [Q921Q3-1] 296222 [Q921Q3-2] |
PTM databases
iPTMneti | Q921Q3 |
PhosphoSitePlusi | Q921Q3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039427, Expressed in cardiac ventricle and 292 other tissues |
Genevisiblei | Q921Q3, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 228964, 3 interactors |
STRINGi | 10090.ENSMUSP00000097770 |
Miscellaneous databases
RNActi | Q921Q3, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2941, Eukaryota |
GeneTreei | ENSGT00390000008647 |
HOGENOMi | CLU_012079_0_0_1 |
InParanoidi | Q921Q3 |
OMAi | PLKVLWQ |
OrthoDBi | 816895at2759 |
PhylomeDBi | Q921Q3 |
TreeFami | TF314121 |
Family and domain databases
InterProi | View protein in InterPro IPR026051, ALG1-like IPR001296, Glyco_trans_1 |
PANTHERi | PTHR13036, PTHR13036, 1 hit |
Pfami | View protein in Pfam PF00534, Glycos_transf_1, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q921Q3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ
60 70 80 90 100
YHALSLAQSG FSVTLLGFYN SKPRDELLQN DRIRIVKLTD LRGLGAGPRI
110 120 130 140 150
LQYGVKVVFQ AVYLLWKMMR MDPAAYIFLQ NPPGLPAIAV CWFVGCICGS
160 170 180 190 200
KLVIDWHNYG YSIMGLVHGP RHPIVLLAKW YEKFFGRLSH LNLCVTNAMR
210 220 230 240 250
EDLAENWCVR AVTLYDKPAS FFKETPLDLQ HELFMKLSHT YSPFQSCSDP
260 270 280 290 300
SHPDTERSAF TERDCQSGVV RRLHGRPALL VSSTSWTEDE DFSILLRALE
310 320 330 340 350
KFEQQALTGD SLPSLVCVIT GKGPLREHYR HLISQKHLQH VRFCTPWLEA
360 370 380 390 400
EDYPLLLGSA DLGVCLHMSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL
410 420 430 440 450
VRHGENGLVF KDAEELAAQL QMLFSKFPDP AGKLSQFRKK LQESGQQRWD
460 470 480
ESWQHTVLPL LAHSQMTPRP HPPCGHPSCR GF
Sequence cautioni
The sequence BC011281 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 339 – 341 | QHV → RHI in BC011281 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 445 | G → R in BC011281 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032516 | 70 – 96 | NSKPR…RGLGA → T in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK145871 mRNA Translation: BAE26713.1 BC011281 mRNA No translation available. |
CCDSi | CCDS49753.1 [Q921Q3-1] |
RefSeqi | NP_663337.2, NM_145362.2 [Q921Q3-1] |
Genome annotation databases
Ensembli | ENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427 [Q921Q3-2] ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427 [Q921Q3-1] |
GeneIDi | 208211 |
KEGGi | mmu:208211 |
UCSCi | uc007yby.2, mouse [Q921Q3-1] uc012aba.2, mouse [Q921Q3-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK145871 mRNA Translation: BAE26713.1 BC011281 mRNA No translation available. |
CCDSi | CCDS49753.1 [Q921Q3-1] |
RefSeqi | NP_663337.2, NM_145362.2 [Q921Q3-1] |
3D structure databases
AlphaFoldDBi | Q921Q3 |
SMRi | Q921Q3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228964, 3 interactors |
STRINGi | 10090.ENSMUSP00000097770 |
Protein family/group databases
CAZyi | GT33, Glycosyltransferase Family 33 |
PTM databases
iPTMneti | Q921Q3 |
PhosphoSitePlusi | Q921Q3 |
Proteomic databases
EPDi | Q921Q3 |
MaxQBi | Q921Q3 |
PaxDbi | Q921Q3 |
PeptideAtlasi | Q921Q3 |
PRIDEi | Q921Q3 |
ProteomicsDBi | 296221 [Q921Q3-1] 296222 [Q921Q3-2] |
Protocols and materials databases
DNASUi | 208211 |
Genome annotation databases
Ensembli | ENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427 [Q921Q3-2] ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427 [Q921Q3-1] |
GeneIDi | 208211 |
KEGGi | mmu:208211 |
UCSCi | uc007yby.2, mouse [Q921Q3-1] uc012aba.2, mouse [Q921Q3-2] |
Organism-specific databases
CTDi | 56052 |
MGIi | MGI:2384774, Alg1 |
VEuPathDBi | HostDB:ENSMUSG00000039427 |
Phylogenomic databases
eggNOGi | KOG2941, Eukaryota |
GeneTreei | ENSGT00390000008647 |
HOGENOMi | CLU_012079_0_0_1 |
InParanoidi | Q921Q3 |
OMAi | PLKVLWQ |
OrthoDBi | 816895at2759 |
PhylomeDBi | Q921Q3 |
TreeFami | TF314121 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
BioGRID-ORCSi | 208211, 29 hits in 77 CRISPR screens |
ChiTaRSi | Alg1, mouse |
PROi | PR:Q921Q3 |
RNActi | Q921Q3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039427, Expressed in cardiac ventricle and 292 other tissues |
Genevisiblei | Q921Q3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026051, ALG1-like IPR001296, Glyco_trans_1 |
PANTHERi | PTHR13036, PTHR13036, 1 hit |
Pfami | View protein in Pfam PF00534, Glycos_transf_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG1_MOUSE | |
Accessioni | Q921Q3Primary (citable) accession number: Q921Q3 Secondary accession number(s): Q3UKT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | October 13, 2009 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families