Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable ATP-dependent RNA helicase DDX27

Gene

Ddx27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi228 – 235ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX27 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 27
Gene namesi
Name:Ddx27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2385884 Ddx27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550321 – 760Probable ATP-dependent RNA helicase DDX27Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Modified residuei25PhosphoserineCombined sources1
Modified residuei48PhosphoserineBy similarity1
Modified residuei133PhosphoserineBy similarity1
Modified residuei144PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ921N6
PaxDbiQ921N6
PeptideAtlasiQ921N6
PRIDEiQ921N6

PTM databases

iPTMnetiQ921N6
PhosphoSitePlusiQ921N6

Expressioni

Gene expression databases

BgeeiENSMUSG00000017999 Expressed in 284 organ(s), highest expression level in ear
CleanExiMM_DDX27
ExpressionAtlasiQ921N6 baseline and differential
GenevisibleiQ921N6 MM

Interactioni

Subunit structurei

Associates with PeBoW complex, composed of BOP1, PES1 and WDR12. Interacts directly with BOP1 and PES1.By similarity

Protein-protein interaction databases

BioGridi230792, 7 interactors
CORUMiQ921N6
IntActiQ921N6, 1 interactor
STRINGi10090.ENSMUSP00000018143

Structurei

3D structure databases

ProteinModelPortaliQ921N6
SMRiQ921N6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 389Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini419 – 569Helicase C-terminalPROSITE-ProRule annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 57Required for interaction with the PEBOW complexBy similarity3
Motifi157 – 166Nuclear localization signalSequence analysis10
Motifi184 – 212Q motifAdd BLAST29
Motifi337 – 340DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 182Asp/Glu/Lys-richAdd BLAST170

Domaini

The C-terminal domain regulates nucleolar localization.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0338 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00550000074997
HOGENOMiHOG000265456
HOVERGENiHBG106162
InParanoidiQ921N6
KOiK13181
OMAiGEADRKM
OrthoDBiEOG091G048O
TreeFamiTF314780

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q921N6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD
60 70 80 90 100
FNPDFVFTEK EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE
110 120 130 140 150
DKEAKSGKVE EKEGQADSDL KGQENPGEDE AGSKDEDSET DYSSEDEEIL
160 170 180 190 200
TKADTLKVKE KKKKKKGQAA GGFFEDASEY DKSLSFQDMN LSRPLLKAIT
210 220 230 240 250
AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL PVLERLIYKP
260 270 280 290 300
RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA
310 320 330 340 350
ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ
360 370 380 390 400
MKEIIRMCSH HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF
410 420 430 440 450
LRQEFIRIRP NREGDREAIV AALLMRTFTD HVMLFTQTKK QAHRMHILLG
460 470 480 490 500
LLGLQVGELH GNLSQTQRLE ALRRFKDEQI DILVATDVAA RGLDIEGVKT
510 520 530 540 550
VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM LKEIVKAAKA
560 570 580 590 600
PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ
610 620 630 640 650
RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF
660 670 680 690 700
MKDAKKKGEM TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG
710 720 730 740 750
PAKKQKQQQK SVFDEELTNT SKKALKQYRA GPSFEERKQS GLPRQRRGNF
760
KSKSRYKRKK
Length:760
Mass (Da):85,939
Last modified:July 27, 2011 - v3
Checksum:iC351B13DAC1B1E0B
GO
Isoform 2 (identifier: Q921N6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-292: G → E
     293-760: Missing.

Note: No experimental confirmation available.
Show »
Length:292
Mass (Da):32,023
Checksum:iEA75C256EE2A0977
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BLJ6H3BLJ6_MOUSE
MCG14602, isoform CRA_b
Ddx27 mCG_14602
310Annotation score:

Sequence cautioni

The sequence AAH11321 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341R → K in AAH11321 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007073292G → E in isoform 2. 1 Publication1
Alternative sequenceiVSP_007074293 – 760Missing in isoform 2. 1 PublicationAdd BLAST468

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134274 mRNA Translation: BAE22077.1
AK138442 mRNA Translation: BAE23664.1
AL591711 Genomic DNA Translation: CAM17306.1
CH466551 Genomic DNA Translation: EDL06495.1
BC011321 mRNA Translation: AAH11321.1 Different initiation.
BC024730 mRNA Translation: AAH24730.1
BC026381 mRNA Translation: AAH26381.1
CCDSiCCDS38336.1 [Q921N6-1]
RefSeqiNP_694705.2, NM_153065.3 [Q921N6-1]
UniGeneiMm.295031

Genome annotation databases

EnsembliENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999 [Q921N6-1]
ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999 [Q921N6-2]
GeneIDi228889
KEGGimmu:228889
UCSCiuc008nza.1 mouse [Q921N6-2]
uc008nzb.1 mouse [Q921N6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134274 mRNA Translation: BAE22077.1
AK138442 mRNA Translation: BAE23664.1
AL591711 Genomic DNA Translation: CAM17306.1
CH466551 Genomic DNA Translation: EDL06495.1
BC011321 mRNA Translation: AAH11321.1 Different initiation.
BC024730 mRNA Translation: AAH24730.1
BC026381 mRNA Translation: AAH26381.1
CCDSiCCDS38336.1 [Q921N6-1]
RefSeqiNP_694705.2, NM_153065.3 [Q921N6-1]
UniGeneiMm.295031

3D structure databases

ProteinModelPortaliQ921N6
SMRiQ921N6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230792, 7 interactors
CORUMiQ921N6
IntActiQ921N6, 1 interactor
STRINGi10090.ENSMUSP00000018143

PTM databases

iPTMnetiQ921N6
PhosphoSitePlusiQ921N6

Proteomic databases

EPDiQ921N6
PaxDbiQ921N6
PeptideAtlasiQ921N6
PRIDEiQ921N6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999 [Q921N6-1]
ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999 [Q921N6-2]
GeneIDi228889
KEGGimmu:228889
UCSCiuc008nza.1 mouse [Q921N6-2]
uc008nzb.1 mouse [Q921N6-1]

Organism-specific databases

CTDi55661
MGIiMGI:2385884 Ddx27

Phylogenomic databases

eggNOGiKOG0338 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00550000074997
HOGENOMiHOG000265456
HOVERGENiHBG106162
InParanoidiQ921N6
KOiK13181
OMAiGEADRKM
OrthoDBiEOG091G048O
TreeFamiTF314780

Miscellaneous databases

PROiPR:Q921N6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017999 Expressed in 284 organ(s), highest expression level in ear
CleanExiMM_DDX27
ExpressionAtlasiQ921N6 baseline and differential
GenevisibleiQ921N6 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDX27_MOUSE
AccessioniPrimary (citable) accession number: Q921N6
Secondary accession number(s): Q3UUG2, Q8R0W3, Q8R1E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again