UniProtKB - Q921M7 (CYRIB_MOUSE)
Protein
CYFIP-related Rac1 interactor B
Gene
Cyrib
Organism
Mus musculus (Mouse)
Status
Functioni
Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (By similarity). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (PubMed:31285585). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).By similarity2 Publications
GO - Molecular functioni
- MHC class Ib protein binding, via antigen binding groove Source: UniProtKB
- small GTPase binding Source: MGI
GO - Biological processi
- cellular response to molecule of bacterial origin Source: UniProtKB
- negative regulation of actin filament polymerization Source: UniProtKB
- negative regulation of small GTPase mediated signal transduction Source: UniProtKB
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of memory T cell activation Source: UniProtKB
- positive regulation of T cell activation Source: UniProtKB
- positive regulation of T cell mediated cytotoxicity Source: UniProtKB
- regulation of cell migration Source: UniProtKB
- regulation of chemotaxis Source: UniProtKB
- regulation of establishment of cell polarity Source: UniProtKB
- regulation of mitochondrial fission Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: CYFIP-related Rac1 interactor BAlternative name(s): Protein FAM49B |
Gene namesi | Name:Cyrib Synonyms:Cyri1 Publication, Fam49b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923520, Cyrib |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Other locations
- Membrane By similarity; Lipid-anchor By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
Keywords - Cellular componenti
Membrane, MitochondrionPathology & Biotechi
Disruption phenotypei
Myeloid-specific conditional knockout mice have a reduced survival following Salmonella systemic infection. Upon infection, they show increased levels of several serum pro-inflammatory cytokines and chemokines produced by activated neutrophils and monocytes.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000187061 | 2 – 324 | CYFIP-related Rac1 interactor BAdd BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Cross-linki | 74 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Post-translational modificationi
Ubiquitinated at Lys-74 upon Salmonella bacterial infection.By similarity
Keywords - PTMi
Isopeptide bond, Lipoprotein, Myristate, Ubl conjugationProteomic databases
EPDi | Q921M7 |
MaxQBi | Q921M7 |
PaxDbi | Q921M7 |
PeptideAtlasi | Q921M7 |
PRIDEi | Q921M7 |
ProteomicsDBi | 277027 |
PTM databases
GlyConnecti | 2631, 1 N-Linked glycan (1 site) |
GlyGeni | Q921M7, 1 site |
iPTMneti | Q921M7 |
PhosphoSitePlusi | Q921M7 |
SwissPalmi | Q921M7 |
Expressioni
Tissue specificityi
Expressed in pancreatic ducts (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000022378, Expressed in granulocyte and 292 other tissues |
ExpressionAtlasi | Q921M7, baseline and differential |
Genevisiblei | Q921M7, MM |
Interactioni
Subunit structurei
Interacts with RAC1 (GTP-bound form preferentially).
By similarityGO - Molecular functioni
- MHC class Ib protein binding, via antigen binding groove Source: UniProtKB
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230158, 4 interactors |
IntActi | Q921M7, 4 interactors |
MINTi | Q921M7 |
STRINGi | 10090.ENSMUSP00000066359 |
Miscellaneous databases
RNActi | Q921M7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the CYRI family.Curated
Phylogenomic databases
eggNOGi | KOG3951, Eukaryota |
GeneTreei | ENSGT00390000015159 |
HOGENOMi | CLU_056470_0_0_1 |
InParanoidi | Q921M7 |
OMAi | TTIHLND |
OrthoDBi | 1129868at2759 |
PhylomeDBi | Q921M7 |
TreeFami | TF314541 |
Family and domain databases
InterProi | View protein in InterPro IPR039789, CYRI IPR009828, DUF1394 |
PANTHERi | PTHR12422, PTHR12422, 1 hit |
Pfami | View protein in Pfam PF07159, DUF1394, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q921M7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNLLKVLTC TDLEQGPNFF LDFENAQPTE SEKEIYNQVN VVLKDAEGIL
60 70 80 90 100
EDLQSYRGAG HEIREAIQHP ADEKLQEKAW GAVVPLVGKL KKFYEFSQRL
110 120 130 140 150
EAALRGLLGA LTSTPYSPTQ HLEREQALAK QFAEILHFTL RFDELKMTNP
160 170 180 190 200
AIQNDFSYYR RTLSRMRINN VPAEGENEVN NELANRMSLF YAEATPMLKT
210 220 230 240 250
LSDATTKFVS ENKNLPIENT TDCLSTMASV CRVMLETPEY RSRFTNEETV
260 270 280 290 300
SFCLRVMVGV IILYDHVHPV GAFAKTSKID MKGCIKVLKD QPPNSVEGLL
310 320
NALRYTTKHL NDETTSKQIR SMLQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BPH9 | A0A2I3BPH9_MOUSE | CYFIP-related Rac1 interactor B | Cyrib | 114 | Annotation score: | ||
A0A2I3BRN5 | A0A2I3BRN5_MOUSE | CYFIP-related Rac1 interactor B | Cyrib | 190 | Annotation score: | ||
A0A2I3BQK1 | A0A2I3BQK1_MOUSE | CYFIP-related Rac1 interactor B | Cyrib | 76 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028062 mRNA Translation: BAC25731.1 AK075613 mRNA Translation: BAC35858.1 BC011343 mRNA Translation: AAH11343.1 |
CCDSi | CCDS27505.1 |
RefSeqi | NP_659095.1, NM_144846.5 XP_006520824.1, XM_006520761.2 XP_006520826.1, XM_006520763.2 XP_006520827.1, XM_006520764.3 XP_011243865.1, XM_011245563.1 XP_011243867.1, XM_011245565.2 |
Genome annotation databases
Ensembli | ENSMUST00000063838; ENSMUSP00000066359; ENSMUSG00000022378 ENSMUST00000164532; ENSMUSP00000132486; ENSMUSG00000022378 ENSMUST00000228226; ENSMUSP00000154320; ENSMUSG00000022378 |
GeneIDi | 223601 |
KEGGi | mmu:223601 |
UCSCi | uc007vze.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028062 mRNA Translation: BAC25731.1 AK075613 mRNA Translation: BAC35858.1 BC011343 mRNA Translation: AAH11343.1 |
CCDSi | CCDS27505.1 |
RefSeqi | NP_659095.1, NM_144846.5 XP_006520824.1, XM_006520761.2 XP_006520826.1, XM_006520763.2 XP_006520827.1, XM_006520764.3 XP_011243865.1, XM_011245563.1 XP_011243867.1, XM_011245565.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7AJL | X-ray | 2.37 | AAA/BBB | 26-324 | [»] | |
SMRi | Q921M7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 230158, 4 interactors |
IntActi | Q921M7, 4 interactors |
MINTi | Q921M7 |
STRINGi | 10090.ENSMUSP00000066359 |
PTM databases
GlyConnecti | 2631, 1 N-Linked glycan (1 site) |
GlyGeni | Q921M7, 1 site |
iPTMneti | Q921M7 |
PhosphoSitePlusi | Q921M7 |
SwissPalmi | Q921M7 |
Proteomic databases
EPDi | Q921M7 |
MaxQBi | Q921M7 |
PaxDbi | Q921M7 |
PeptideAtlasi | Q921M7 |
PRIDEi | Q921M7 |
ProteomicsDBi | 277027 |
Protocols and materials databases
Antibodypediai | 2198, 79 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000063838; ENSMUSP00000066359; ENSMUSG00000022378 ENSMUST00000164532; ENSMUSP00000132486; ENSMUSG00000022378 ENSMUST00000228226; ENSMUSP00000154320; ENSMUSG00000022378 |
GeneIDi | 223601 |
KEGGi | mmu:223601 |
UCSCi | uc007vze.2, mouse |
Organism-specific databases
CTDi | 51571 |
MGIi | MGI:1923520, Cyrib |
Phylogenomic databases
eggNOGi | KOG3951, Eukaryota |
GeneTreei | ENSGT00390000015159 |
HOGENOMi | CLU_056470_0_0_1 |
InParanoidi | Q921M7 |
OMAi | TTIHLND |
OrthoDBi | 1129868at2759 |
PhylomeDBi | Q921M7 |
TreeFami | TF314541 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Miscellaneous databases
BioGRID-ORCSi | 223601, 3 hits in 53 CRISPR screens |
ChiTaRSi | Fam49b, mouse |
PROi | PR:Q921M7 |
RNActi | Q921M7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022378, Expressed in granulocyte and 292 other tissues |
ExpressionAtlasi | Q921M7, baseline and differential |
Genevisiblei | Q921M7, MM |
Family and domain databases
InterProi | View protein in InterPro IPR039789, CYRI IPR009828, DUF1394 |
PANTHERi | PTHR12422, PTHR12422, 1 hit |
Pfami | View protein in Pfam PF07159, DUF1394, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYRIB_MOUSE | |
Accessioni | Q921M7Primary (citable) accession number: Q921M7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families