UniProtKB - Q921J2 (RHEB_MOUSE)
Protein
GTP-binding protein Rheb
Gene
Rheb
Organism
Mus musculus (Mouse)
Status
Functioni
Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.Curated1 Publication
Miscellaneous
The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB.By similarity
Activity regulationi
Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | Magnesium1 Publication | 1 | |
Metal bindingi | 38 | MagnesiumBy similarity | 1 | |
Binding sitei | 63 | GTP; via amide nitrogen | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 16 – 21 | GTP1 Publication | 6 | |
Nucleotide bindingi | 32 – 38 | GTP1 Publication | 7 | |
Nucleotide bindingi | 119 – 122 | GTP1 Publication | 4 | |
Nucleotide bindingi | 149 – 150 | GTP1 Publication | 2 |
GO - Molecular functioni
- GDP binding Source: MGI
- GTPase activity Source: MGI
- GTP binding Source: UniProtKB
- magnesium ion binding Source: MGI
- protein kinase binding Source: UniProtKB
GO - Biological processi
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of oligodendrocyte differentiation Source: MGI
- positive regulation of TOR signaling Source: UniProtKB
- regulation of postsynapse organization Source: MGI
- regulation of TOR signaling Source: CACAO
- regulation of type B pancreatic cell development Source: MGI
- small GTPase mediated signal transduction Source: MGI
Keywordsi
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-165159, MTOR signalling R-MMU-166208, mTORC1-mediated signalling R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-9639288, Amino acids regulate mTORC1 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding protein RhebAlternative name(s): Ras homolog enriched in brain |
Gene namesi | Name:Rheb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97912, Rheb |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol By similarity
Other locations
- Endomembrane system By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- spliceosomal complex Source: MGI
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- dendrite Source: MGI
- glutamatergic synapse Source: MGI
- neuronal cell body Source: MGI
- postsynaptic density Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 63 | G → A: Impairs GTPase activity and increases stimulation of the protein kinase activity of mTORC1. 1 Publication | 1 | |
Mutagenesisi | 130 | S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082709 | 1 – 181 | GTP-binding protein RhebAdd BLAST | 181 | |
PropeptideiPRO_0000281366 | 182 – 184 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 130 | Phosphoserine; by MAPKAPK51 Publication | 1 | |
Modified residuei | 181 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 181 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Farnesylation is important for efficiently activating mTORC1-mediated signaling.By similarity
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.1 Publication
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | Q921J2 |
MaxQBi | Q921J2 |
PaxDbi | Q921J2 |
PRIDEi | Q921J2 |
PTM databases
iPTMneti | Q921J2 |
PhosphoSitePlusi | Q921J2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028945, Expressed in CA1 field of hippocampus and 290 other tissues |
Genevisiblei | Q921J2, MM |
Interactioni
Subunit structurei
Binds to mTORC1 in a guanyl nucleotide-independent manner.
Interacts directly with MTOR, MLST8 and RPTOR.
Interacts with TSC2 (By similarity).
Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202889, 15 interactors |
IntActi | Q921J2, 4 interactors |
MINTi | Q921J2 |
STRINGi | 10090.ENSMUSP00000030787 |
Miscellaneous databases
RNActi | Q921J2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q921J2 |
SMRi | Q921J2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 35 – 43 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000159945 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q921J2 |
OMAi | FIENHFV |
OrthoDBi | 1320427at2759 |
PhylomeDBi | Q921J2 |
TreeFami | TF314986 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR037586, RHEB IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF264, PTHR24070:SF264, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q921J2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN
60 70 80 90 100
GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH
110 120 130 140 150
GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA
160 170 180
KENQTAVDVF KRIILEAEKI DGAASQGKSS CSVM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC012273 mRNA Translation: AAH12273.1 |
CCDSi | CCDS39032.1 |
RefSeqi | NP_444305.2, NM_053075.3 |
Genome annotation databases
Ensembli | ENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945 |
GeneIDi | 19744 |
KEGGi | mmu:19744 |
UCSCi | uc008wsj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC012273 mRNA Translation: AAH12273.1 |
CCDSi | CCDS39032.1 |
RefSeqi | NP_444305.2, NM_053075.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4O25 | X-ray | 2.20 | A/B | 1-169 | [»] | |
4O2L | X-ray | 2.40 | A/B | 1-169 | [»] | |
4O2R | X-ray | 2.25 | A/B | 1-169 | [»] | |
BMRBi | Q921J2 | |||||
SMRi | Q921J2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202889, 15 interactors |
IntActi | Q921J2, 4 interactors |
MINTi | Q921J2 |
STRINGi | 10090.ENSMUSP00000030787 |
PTM databases
iPTMneti | Q921J2 |
PhosphoSitePlusi | Q921J2 |
Proteomic databases
EPDi | Q921J2 |
MaxQBi | Q921J2 |
PaxDbi | Q921J2 |
PRIDEi | Q921J2 |
Protocols and materials databases
Antibodypediai | 18765, 461 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945 |
GeneIDi | 19744 |
KEGGi | mmu:19744 |
UCSCi | uc008wsj.1, mouse |
Organism-specific databases
CTDi | 6009 |
MGIi | MGI:97912, Rheb |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000159945 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | Q921J2 |
OMAi | FIENHFV |
OrthoDBi | 1320427at2759 |
PhylomeDBi | Q921J2 |
TreeFami | TF314986 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-165159, MTOR signalling R-MMU-166208, mTORC1-mediated signalling R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-9639288, Amino acids regulate mTORC1 |
Miscellaneous databases
BioGRID-ORCSi | 19744, 5 hits in 18 CRISPR screens |
ChiTaRSi | Rheb, mouse |
PROi | PR:Q921J2 |
RNActi | Q921J2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028945, Expressed in CA1 field of hippocampus and 290 other tissues |
Genevisiblei | Q921J2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR037586, RHEB IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF264, PTHR24070:SF264, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHEB_MOUSE | |
Accessioni | Q921J2Primary (citable) accession number: Q921J2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families