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Protein

SH3 domain-binding protein 4

Gene

Sh3bp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 domain-binding protein 4
Gene namesi
Name:Sh3bp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2138297 Sh3bp4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745751 – 962SH3 domain-binding protein 4Add BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphoserineBy similarity1
Modified residuei245PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei278PhosphoserineBy similarity1
Modified residuei295PhosphoserineCombined sources1
Modified residuei636PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ921I6
PaxDbiQ921I6
PeptideAtlasiQ921I6
PRIDEiQ921I6

PTM databases

iPTMnetiQ921I6
PhosphoSitePlusiQ921I6

Expressioni

Gene expression databases

BgeeiENSMUSG00000036206 Expressed in 227 organ(s), highest expression level in lacrimal gland
CleanExiMM_SH3BP4
GenevisibleiQ921I6 MM

Interactioni

Subunit structurei

Homodimer or homooligomer. Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC. Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221051, 1 interactor
STRINGi10090.ENSMUSP00000067581

Structurei

3D structure databases

ProteinModelPortaliQ921I6
SMRiQ921I6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 114SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini316 – 453ZU5PROSITE-ProRule annotationAdd BLAST138
Domaini653 – 723SH3 2PROSITE-ProRule annotationAdd BLAST71

Domaini

The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity. The SH3 domain also mediates interaction with RRAGB, RRAGC and is required for the negative regulation of mTORC1 (By similarity).By similarity

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IEE9 Eukaryota
ENOG41118AI LUCA
GeneTreeiENSGT00390000013151
HOGENOMiHOG000015297
HOVERGENiHBG057439
InParanoidiQ921I6
KOiK20066
OMAiQSKNHYL
OrthoDBiEOG091G012M
PhylomeDBiQ921I6
TreeFamiTF105572

Family and domain databases

CDDicd11757 SH3_SH3BP4, 1 hit
InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035455 SH3BP4
IPR035456 SH3BP4_SH3
IPR000906 ZU5_dom
PANTHERiPTHR15603:SF3 PTHR15603:SF3, 1 hit
PfamiView protein in Pfam
PF07653 SH3_2, 1 hit
PF14604 SH3_9, 1 hit
PF00791 ZU5, 1 hit
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 2 hits
PS51145 ZU5, 1 hit

Sequencei

Sequence statusi: Complete.

Q921I6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAQRIRAAN ASGLPRCKSE GTLIDLSEGF SETSFNDVKV PSPSALLVDN
60 70 80 90 100
PTPFGNAKEV IAIKDYCPNN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE
110 120 130 140 150
MGYIPSSYVQ PLNYRNSTLS DSGMIDNLPD SPEEVAKELD LLGGGWTDDQ
160 170 180 190 200
KESGRPYSNN PFWNGVRTNP FLNGNAQPST DELNPKSTVD LLLFDTGTSS
210 220 230 240 250
FTESSSATTN STGNIFDELP ATNGLQVEQP VKRDNPFFRS KRSYSLSELS
260 270 280 290 300
VLQAKSDAPP TSSFFTGLKS PVPEQFQSRE DFRTAWLNHR KLARSCHDLD
310 320 330 340 350
LLGQSPGWGQ TQAVETNIVC KLDSSGGSVQ LPDTNISIHV PEGHVAPGET
360 370 380 390 400
QQISMKALLD PPLDLNSDRS TSVSPVVEVK LSNLEVSTFI ILEMKVSAEV
410 420 430 440 450
KGDIFSKSTV VLQCLRSDSK EGPYVPIPLA YSYGDTIQVQ LDNLEPCMYL
460 470 480 490 500
AIVAQGPNIL YPSTVWDFIN KRVTVGLYGP KHIHPSFKTV VTIFGHDCAP
510 520 530 540 550
KTLLVSEVTR QAPSPAPVAL QLWGKHQFIL SRPQDLRVCM FSNMTNYEVK
560 570 580 590 600
ANEQARVVRG FQMKLGKVSR LIFSVISQNP NELSDFTLRV QVKDDQDTIL
610 620 630 640 650
TQFCVQTPQP PPKSAIKPSG QRRFLKKNEV GKIILSPFVV TTKYPTFQDR
660 670 680 690 700
PVSSLKFGKL LKTVVRQNKS HYLLEYKKGD VVALLSEERI RLKGQLWTKE
710 720 730 740 750
WYIGYYQGKV GLVHTKNVLV VGKARPSLFS GPELSTSVLL EQILRPCKFL
760 770 780 790 800
TYIYASVRTL LMENISSWRA FADALGYGNL PLTFFCRAEL DSEPERVASV
810 820 830 840 850
LEKLKEDCNN PDNKDRKSFQ KELVMALLKM DCQGLVVRLI QDFVLLTTAV
860 870 880 890 900
EVAQRWRELA EKLAKVSKQQ MDAYESPHRD RNGVVDSEAM WKPAYDFLLT
910 920 930 940 950
WSHQIGDSYR DVIQELHIGL DKMKNPITRR WKHLTGTLIL VNSLDILRAA
960
AFSPADHDDF VI
Length:962
Mass (Da):107,584
Last modified:December 1, 2001 - v1
Checksum:i11154849455F4063
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312Q → K in BAC30966 (PubMed:16141072).Curated1
Sequence conflicti340V → E in BAC27961 (PubMed:16141072).Curated1
Sequence conflicti534Q → K in BAC27961 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028826 mRNA Translation: BAC26140.1
AK032634 mRNA Translation: BAC27961.1
AK041504 mRNA Translation: BAC30966.1
AK043259 mRNA Translation: BAC31506.1
BC012284 mRNA Translation: AAH12284.1
BC034733 mRNA Translation: AAH34733.1
CCDSiCCDS15147.1
RefSeqiNP_598577.1, NM_133816.2
XP_006530038.1, XM_006529975.3
UniGeneiMm.170983

Genome annotation databases

EnsembliENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206
GeneIDi98402
KEGGimmu:98402
UCSCiuc007byv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028826 mRNA Translation: BAC26140.1
AK032634 mRNA Translation: BAC27961.1
AK041504 mRNA Translation: BAC30966.1
AK043259 mRNA Translation: BAC31506.1
BC012284 mRNA Translation: AAH12284.1
BC034733 mRNA Translation: AAH34733.1
CCDSiCCDS15147.1
RefSeqiNP_598577.1, NM_133816.2
XP_006530038.1, XM_006529975.3
UniGeneiMm.170983

3D structure databases

ProteinModelPortaliQ921I6
SMRiQ921I6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221051, 1 interactor
STRINGi10090.ENSMUSP00000067581

PTM databases

iPTMnetiQ921I6
PhosphoSitePlusiQ921I6

Proteomic databases

MaxQBiQ921I6
PaxDbiQ921I6
PeptideAtlasiQ921I6
PRIDEiQ921I6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206
GeneIDi98402
KEGGimmu:98402
UCSCiuc007byv.1 mouse

Organism-specific databases

CTDi23677
MGIiMGI:2138297 Sh3bp4

Phylogenomic databases

eggNOGiENOG410IEE9 Eukaryota
ENOG41118AI LUCA
GeneTreeiENSGT00390000013151
HOGENOMiHOG000015297
HOVERGENiHBG057439
InParanoidiQ921I6
KOiK20066
OMAiQSKNHYL
OrthoDBiEOG091G012M
PhylomeDBiQ921I6
TreeFamiTF105572

Miscellaneous databases

ChiTaRSiSh3bp4 mouse
PROiPR:Q921I6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036206 Expressed in 227 organ(s), highest expression level in lacrimal gland
CleanExiMM_SH3BP4
GenevisibleiQ921I6 MM

Family and domain databases

CDDicd11757 SH3_SH3BP4, 1 hit
InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035455 SH3BP4
IPR035456 SH3BP4_SH3
IPR000906 ZU5_dom
PANTHERiPTHR15603:SF3 PTHR15603:SF3, 1 hit
PfamiView protein in Pfam
PF07653 SH3_2, 1 hit
PF14604 SH3_9, 1 hit
PF00791 ZU5, 1 hit
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 2 hits
PS51145 ZU5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSH3B4_MOUSE
AccessioniPrimary (citable) accession number: Q921I6
Secondary accession number(s): Q8BXV5, Q8BY95, Q8C007
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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