Sh3bp4

Functionⁱ

May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).By similarity

GO - Molecular functionⁱ

GDP-dissociation inhibitor activity Source: UniProtKB
identical protein binding Source: MGI
Ras GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to amino acid stimulus Source: UniProtKB
endocytosis Source: UniProtKB-KW
negative regulation of cell growth Source: UniProtKB
negative regulation of cell proliferation Source: UniProtKB
negative regulation of GTPase activity Source: UniProtKB
negative regulation of TOR signaling Source: UniProtKB
positive regulation of autophagy Source: UniProtKB
protein localization to lysosome Source: UniProtKB
regulation of catalytic activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Endocytosis

Biological process

Endocytosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SH3 domain-binding protein 4
Gene namesⁱ	Name:Sh3bp4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:2138297 Sh3bp4

Keywords - Cellular componentⁱ

Coated pit, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000274575	1 – 962	SH3 domain-binding protein 4Add BLAST		962

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	131	PhosphoserineBy similarity	1
Modified residueⁱ	245	PhosphoserineCombined sources	1
Modified residueⁱ	250	PhosphoserineCombined sources	1
Modified residueⁱ	278	PhosphoserineBy similarity	1
Modified residueⁱ	295	PhosphoserineCombined sources	1
Modified residueⁱ	636	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2 (By similarity).By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q921I6
PaxDbⁱ	Q921I6
PeptideAtlas More...PeptideAtlasⁱ	Q921I6
PRIDEⁱ	Q921I6

PTM databases

iPTMnetⁱ	Q921I6
PhosphoSitePlusⁱ	Q921I6

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000036206 Expressed in 227 organ(s), highest expression level in lacrimal gland
CleanExⁱ	MM_SH3BP4
Genevisibleⁱ	Q921I6 MM

Interactionⁱ

Subunit structureⁱ

Homodimer or homooligomer. Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC. Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids (By similarity).By similarity

GO - Molecular functionⁱ

identical protein binding Source: MGI
Ras GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	221051, 1 interactor
STRINGⁱ	10090.ENSMUSP00000067581

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q921I6
SMRⁱ	Q921I6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	55 – 114	SH3 1PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	316 – 453	ZU5PROSITE-ProRule annotationAdd BLAST	138
Domainⁱ	653 – 723	SH3 2PROSITE-ProRule annotationAdd BLAST	71

Domainⁱ

The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity. The SH3 domain also mediates interaction with RRAGB, RRAGC and is required for the negative regulation of mTORC1 (By similarity).By similarity

Keywords - Domainⁱ

Repeat, SH3 domain

Phylogenomic databases

eggNOGⁱ	ENOG410IEE9 Eukaryota ENOG41118AI LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000013151
HOGENOMⁱ	HOG000015297
HOVERGENⁱ	HBG057439
InParanoidⁱ	Q921I6
KEGG Orthology (KO) More...KOⁱ	K20066
OMAⁱ	QSKNHYL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G012M
PhylomeDBⁱ	Q921I6
TreeFamⁱ	TF105572

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd11757 SH3_SH3BP4, 1 hit
InterProⁱ	View protein in InterPro IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR035455 SH3BP4 IPR035456 SH3BP4_SH3 IPR000906 ZU5_dom
PANTHERⁱ	PTHR15603:SF3 PTHR15603:SF3, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07653 SH3_2, 1 hit PF14604 SH3_9, 1 hit PF00791 ZU5, 1 hit
SMARTⁱ	View protein in SMART SM00326 SH3, 1 hit
SUPFAMⁱ	SSF50044 SSF50044, 1 hit
PROSITEⁱ	View protein in PROSITE PS50002 SH3, 2 hits PS51145 ZU5, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q921I6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAQRIRAAN ASGLPRCKSE GTLIDLSEGF SETSFNDVKV PSPSALLVDN 
        60         70         80         90        100
PTPFGNAKEV IAIKDYCPNN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE 
       110        120        130        140        150
MGYIPSSYVQ PLNYRNSTLS DSGMIDNLPD SPEEVAKELD LLGGGWTDDQ 
       160        170        180        190        200
KESGRPYSNN PFWNGVRTNP FLNGNAQPST DELNPKSTVD LLLFDTGTSS 
       210        220        230        240        250
FTESSSATTN STGNIFDELP ATNGLQVEQP VKRDNPFFRS KRSYSLSELS 
       260        270        280        290        300
VLQAKSDAPP TSSFFTGLKS PVPEQFQSRE DFRTAWLNHR KLARSCHDLD 
       310        320        330        340        350
LLGQSPGWGQ TQAVETNIVC KLDSSGGSVQ LPDTNISIHV PEGHVAPGET 
       360        370        380        390        400
QQISMKALLD PPLDLNSDRS TSVSPVVEVK LSNLEVSTFI ILEMKVSAEV 
       410        420        430        440        450
KGDIFSKSTV VLQCLRSDSK EGPYVPIPLA YSYGDTIQVQ LDNLEPCMYL 
       460        470        480        490        500
AIVAQGPNIL YPSTVWDFIN KRVTVGLYGP KHIHPSFKTV VTIFGHDCAP 
       510        520        530        540        550
KTLLVSEVTR QAPSPAPVAL QLWGKHQFIL SRPQDLRVCM FSNMTNYEVK 
       560        570        580        590        600
ANEQARVVRG FQMKLGKVSR LIFSVISQNP NELSDFTLRV QVKDDQDTIL 
       610        620        630        640        650
TQFCVQTPQP PPKSAIKPSG QRRFLKKNEV GKIILSPFVV TTKYPTFQDR 
       660        670        680        690        700
PVSSLKFGKL LKTVVRQNKS HYLLEYKKGD VVALLSEERI RLKGQLWTKE 
       710        720        730        740        750
WYIGYYQGKV GLVHTKNVLV VGKARPSLFS GPELSTSVLL EQILRPCKFL 
       760        770        780        790        800
TYIYASVRTL LMENISSWRA FADALGYGNL PLTFFCRAEL DSEPERVASV 
       810        820        830        840        850
LEKLKEDCNN PDNKDRKSFQ KELVMALLKM DCQGLVVRLI QDFVLLTTAV 
       860        870        880        890        900
EVAQRWRELA EKLAKVSKQQ MDAYESPHRD RNGVVDSEAM WKPAYDFLLT 
       910        920        930        940        950
WSHQIGDSYR DVIQELHIGL DKMKNPITRR WKHLTGTLIL VNSLDILRAA 
       960 
AFSPADHDDF VI

Length:962

Mass (Da):107,584

Last modified:December 1, 2001 - v1

Checksum:ⁱ11154849455F4063

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	312	Q → K in BAC30966 (PubMed:16141072).Curated	1
Sequence conflictⁱ	340	V → E in BAC27961 (PubMed:16141072).Curated	1
Sequence conflictⁱ	534	Q → K in BAC27961 (PubMed:16141072).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK028826 mRNA Translation: BAC26140.1 AK032634 mRNA Translation: BAC27961.1 AK041504 mRNA Translation: BAC30966.1 AK043259 mRNA Translation: BAC31506.1 BC012284 mRNA Translation: AAH12284.1 BC034733 mRNA Translation: AAH34733.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15147.1
NCBI Reference Sequences More...RefSeqⁱ	NP_598577.1, NM_133816.2 XP_006530038.1, XM_006529975.3
UniGeneⁱ	Mm.170983

Genome annotation databases

Ensemblⁱ	ENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206
GeneIDⁱ	98402
KEGGⁱ	mmu:98402
UCSC genome browser More...UCSCⁱ	uc007byv.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q921I6	SH3 domain-binding protein 4		HUMAN		963	UniRef90_Q9P0V3
SH3 domain-binding protein 4		RAT		961
SH3 domain-binding protein 4		CRIGR		1096
Uncharacterized protein		AILME		971
SH3 domain binding protein 4		HORSE		964
+112

Protein	Similar proteins		Species	Score	Length	Source
Q921I6	SH3 domain-binding protein 4		HUMAN		963	UniRef50_Q9P0V3
SH3 domain-binding protein 4		RAT		961
SH3 domain-binding protein 4		SERQU		966
SH3 domain-binding protein 4-A		DANRE		965
SH3 domain-binding protein 4-A		XENLA		957
+468

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK028826 mRNA Translation: BAC26140.1 AK032634 mRNA Translation: BAC27961.1 AK041504 mRNA Translation: BAC30966.1 AK043259 mRNA Translation: BAC31506.1 BC012284 mRNA Translation: AAH12284.1 BC034733 mRNA Translation: AAH34733.1
CCDSⁱ	CCDS15147.1
RefSeqⁱ	NP_598577.1, NM_133816.2 XP_006530038.1, XM_006529975.3
UniGeneⁱ	Mm.170983

3D structure databases

ProteinModelPortalⁱ	Q921I6
SMRⁱ	Q921I6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	221051, 1 interactor
STRINGⁱ	10090.ENSMUSP00000067581

PTM databases

iPTMnetⁱ	Q921I6
PhosphoSitePlusⁱ	Q921I6

Proteomic databases

MaxQBⁱ	Q921I6
PaxDbⁱ	Q921I6
PeptideAtlasⁱ	Q921I6
PRIDEⁱ	Q921I6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206
GeneIDⁱ	98402
KEGGⁱ	mmu:98402
UCSCⁱ	uc007byv.1 mouse

Organism-specific databases

CTDⁱ	23677
MGIⁱ	MGI:2138297 Sh3bp4

Phylogenomic databases

eggNOGⁱ	ENOG410IEE9 Eukaryota ENOG41118AI LUCA
GeneTreeⁱ	ENSGT00390000013151
HOGENOMⁱ	HOG000015297
HOVERGENⁱ	HBG057439
InParanoidⁱ	Q921I6
KOⁱ	K20066
OMAⁱ	QSKNHYL
OrthoDBⁱ	EOG091G012M
PhylomeDBⁱ	Q921I6
TreeFamⁱ	TF105572

Miscellaneous databases

ChiTaRSⁱ	Sh3bp4 mouse
Protein Ontology More...PROⁱ	PR:Q921I6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000036206 Expressed in 227 organ(s), highest expression level in lacrimal gland
CleanExⁱ	MM_SH3BP4
Genevisibleⁱ	Q921I6 MM

Family and domain databases

CDDⁱ	cd11757 SH3_SH3BP4, 1 hit
InterProⁱ	View protein in InterPro IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR035455 SH3BP4 IPR035456 SH3BP4_SH3 IPR000906 ZU5_dom
PANTHERⁱ	PTHR15603:SF3 PTHR15603:SF3, 1 hit
Pfamⁱ	View protein in Pfam PF07653 SH3_2, 1 hit PF14604 SH3_9, 1 hit PF00791 ZU5, 1 hit
SMARTⁱ	View protein in SMART SM00326 SH3, 1 hit
SUPFAMⁱ	SSF50044 SSF50044, 1 hit
PROSITEⁱ	View protein in PROSITE PS50002 SH3, 2 hits PS51145 ZU5, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SH3B4_MOUSE
Accessionⁱ	Q921I6Primary (citable) accession number: Q921I6 Secondary accession number(s): Q8BXV5, Q8BY95, Q8C007
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
	Last sequence update:	December 1, 2001
	Last modified:	November 7, 2018
	This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q921I6 (SH3B4_MOUSE)

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SH3 domain-binding protein 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ