UniProtKB - Q921I6 (SH3B4_MOUSE)
Protein
SH3 domain-binding protein 4
Gene
Sh3bp4
Organism
Mus musculus (Mouse)
Status
Functioni
May function in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis. Alternatively, may act as a negative regulator of the amino acid-induced TOR signaling by inhibiting the formation of active Rag GTPase complexes. Preferentially binds inactive Rag GTPase complexes and prevents their interaction with the mTORC1 complex inhibiting its relocalization to lysosomes and its activation. Thereby, may indirectly regulate cell growth, proliferation and autophagy (By similarity).By similarity
GO - Molecular functioni
- GDP-dissociation inhibitor activity Source: UniProtKB
- identical protein binding Source: MGI
- Ras GTPase binding Source: MGI
GO - Biological processi
- cellular response to amino acid stimulus Source: UniProtKB
- endocytosis Source: UniProtKB-KW
- negative regulation of cell growth Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of GTPase activity Source: UniProtKB
- negative regulation of TOR signaling Source: UniProtKB
- positive regulation of autophagy Source: UniProtKB
- protein localization to lysosome Source: UniProtKB
- regulation of catalytic activity Source: UniProtKB
Keywordsi
Biological process | Endocytosis |
Enzyme and pathway databases
Reactomei | R-MMU-9639288, Amino acids regulate mTORC1 |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 domain-binding protein 4 |
Gene namesi | Name:Sh3bp4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2138297, Sh3bp4 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- clathrin-coated pit By similarity
- clathrin-coated vesicle By similarity
Note: Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus (By similarity).By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- clathrin-coated vesicle Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Coated pit, Cytoplasmic vesicle, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274575 | 1 – 962 | SH3 domain-binding protein 4Add BLAST | 962 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 131 | PhosphoserineBy similarity | 1 | |
Modified residuei | 245 | PhosphoserineCombined sources | 1 | |
Modified residuei | 250 | PhosphoserineCombined sources | 1 | |
Modified residuei | 278 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineCombined sources | 1 | |
Modified residuei | 636 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q921I6 |
MaxQBi | Q921I6 |
PaxDbi | Q921I6 |
PeptideAtlasi | Q921I6 |
PRIDEi | Q921I6 |
PTM databases
iPTMneti | Q921I6 |
PhosphoSitePlusi | Q921I6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036206, Expressed in cortical plate and 248 other tissues |
Genevisiblei | Q921I6, MM |
Interactioni
Subunit structurei
Homodimer or homooligomer.
Interacts with DNM2, EPS15, clathrin, the adapter protein complex 2/AP-2 and TFRC.
Interacts with the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; the interaction is most probably direct, preferentially occurs with their inactive GDP-bound form and is negatively regulated by amino acids (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- Ras GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 221051, 6 interactors |
STRINGi | 10090.ENSMUSP00000067581 |
Miscellaneous databases
RNActi | Q921I6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 114 | SH3 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 316 – 453 | ZU5PROSITE-ProRule annotationAdd BLAST | 138 | |
Domaini | 653 – 723 | SH3 2PROSITE-ProRule annotationAdd BLAST | 71 |
Domaini
The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity. The SH3 domain also mediates interaction with RRAGB, RRAGC and is required for the negative regulation of mTORC1 (By similarity).By similarity
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
eggNOGi | ENOG502QTUW, Eukaryota |
GeneTreei | ENSGT00390000013151 |
HOGENOMi | CLU_013080_2_0_1 |
InParanoidi | Q921I6 |
OMAi | AFSPVDH |
OrthoDBi | 140316at2759 |
PhylomeDBi | Q921I6 |
TreeFami | TF105572 |
Family and domain databases
CDDi | cd11757, SH3_SH3BP4, 1 hit |
InterProi | View protein in InterPro IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR035455, SH3BP4 IPR035456, SH3BP4_SH3 IPR000906, ZU5_dom |
PANTHERi | PTHR15603:SF3, PTHR15603:SF3, 1 hit |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF07653, SH3_2, 1 hit PF00791, ZU5, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 2 hits PS51145, ZU5, 1 hit |
i Sequence
Sequence statusi: Complete.
Q921I6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAQRIRAAN ASGLPRCKSE GTLIDLSEGF SETSFNDVKV PSPSALLVDN
60 70 80 90 100
PTPFGNAKEV IAIKDYCPNN FTTLKFSKGD HLYVLDTSGG EWWYAHNTTE
110 120 130 140 150
MGYIPSSYVQ PLNYRNSTLS DSGMIDNLPD SPEEVAKELD LLGGGWTDDQ
160 170 180 190 200
KESGRPYSNN PFWNGVRTNP FLNGNAQPST DELNPKSTVD LLLFDTGTSS
210 220 230 240 250
FTESSSATTN STGNIFDELP ATNGLQVEQP VKRDNPFFRS KRSYSLSELS
260 270 280 290 300
VLQAKSDAPP TSSFFTGLKS PVPEQFQSRE DFRTAWLNHR KLARSCHDLD
310 320 330 340 350
LLGQSPGWGQ TQAVETNIVC KLDSSGGSVQ LPDTNISIHV PEGHVAPGET
360 370 380 390 400
QQISMKALLD PPLDLNSDRS TSVSPVVEVK LSNLEVSTFI ILEMKVSAEV
410 420 430 440 450
KGDIFSKSTV VLQCLRSDSK EGPYVPIPLA YSYGDTIQVQ LDNLEPCMYL
460 470 480 490 500
AIVAQGPNIL YPSTVWDFIN KRVTVGLYGP KHIHPSFKTV VTIFGHDCAP
510 520 530 540 550
KTLLVSEVTR QAPSPAPVAL QLWGKHQFIL SRPQDLRVCM FSNMTNYEVK
560 570 580 590 600
ANEQARVVRG FQMKLGKVSR LIFSVISQNP NELSDFTLRV QVKDDQDTIL
610 620 630 640 650
TQFCVQTPQP PPKSAIKPSG QRRFLKKNEV GKIILSPFVV TTKYPTFQDR
660 670 680 690 700
PVSSLKFGKL LKTVVRQNKS HYLLEYKKGD VVALLSEERI RLKGQLWTKE
710 720 730 740 750
WYIGYYQGKV GLVHTKNVLV VGKARPSLFS GPELSTSVLL EQILRPCKFL
760 770 780 790 800
TYIYASVRTL LMENISSWRA FADALGYGNL PLTFFCRAEL DSEPERVASV
810 820 830 840 850
LEKLKEDCNN PDNKDRKSFQ KELVMALLKM DCQGLVVRLI QDFVLLTTAV
860 870 880 890 900
EVAQRWRELA EKLAKVSKQQ MDAYESPHRD RNGVVDSEAM WKPAYDFLLT
910 920 930 940 950
WSHQIGDSYR DVIQELHIGL DKMKNPITRR WKHLTGTLIL VNSLDILRAA
960
AFSPADHDDF VI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 312 | Q → K in BAC30966 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 340 | V → E in BAC27961 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 534 | Q → K in BAC27961 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028826 mRNA Translation: BAC26140.1 AK032634 mRNA Translation: BAC27961.1 AK041504 mRNA Translation: BAC30966.1 AK043259 mRNA Translation: BAC31506.1 BC012284 mRNA Translation: AAH12284.1 BC034733 mRNA Translation: AAH34733.1 |
CCDSi | CCDS15147.1 |
RefSeqi | NP_598577.1, NM_133816.2 XP_006530038.1, XM_006529975.3 |
Genome annotation databases
Ensembli | ENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206 |
GeneIDi | 98402 |
KEGGi | mmu:98402 |
UCSCi | uc007byv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028826 mRNA Translation: BAC26140.1 AK032634 mRNA Translation: BAC27961.1 AK041504 mRNA Translation: BAC30966.1 AK043259 mRNA Translation: BAC31506.1 BC012284 mRNA Translation: AAH12284.1 BC034733 mRNA Translation: AAH34733.1 |
CCDSi | CCDS15147.1 |
RefSeqi | NP_598577.1, NM_133816.2 XP_006530038.1, XM_006529975.3 |
3D structure databases
SMRi | Q921I6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221051, 6 interactors |
STRINGi | 10090.ENSMUSP00000067581 |
PTM databases
iPTMneti | Q921I6 |
PhosphoSitePlusi | Q921I6 |
Proteomic databases
jPOSTi | Q921I6 |
MaxQBi | Q921I6 |
PaxDbi | Q921I6 |
PeptideAtlasi | Q921I6 |
PRIDEi | Q921I6 |
Protocols and materials databases
Antibodypediai | 34462, 178 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000066279; ENSMUSP00000067581; ENSMUSG00000036206 |
GeneIDi | 98402 |
KEGGi | mmu:98402 |
UCSCi | uc007byv.1, mouse |
Organism-specific databases
CTDi | 23677 |
MGIi | MGI:2138297, Sh3bp4 |
Phylogenomic databases
eggNOGi | ENOG502QTUW, Eukaryota |
GeneTreei | ENSGT00390000013151 |
HOGENOMi | CLU_013080_2_0_1 |
InParanoidi | Q921I6 |
OMAi | AFSPVDH |
OrthoDBi | 140316at2759 |
PhylomeDBi | Q921I6 |
TreeFami | TF105572 |
Enzyme and pathway databases
Reactomei | R-MMU-9639288, Amino acids regulate mTORC1 |
Miscellaneous databases
BioGRID-ORCSi | 98402, 0 hits in 17 CRISPR screens |
ChiTaRSi | Sh3bp4, mouse |
PROi | PR:Q921I6 |
RNActi | Q921I6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036206, Expressed in cortical plate and 248 other tissues |
Genevisiblei | Q921I6, MM |
Family and domain databases
CDDi | cd11757, SH3_SH3BP4, 1 hit |
InterProi | View protein in InterPro IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR035455, SH3BP4 IPR035456, SH3BP4_SH3 IPR000906, ZU5_dom |
PANTHERi | PTHR15603:SF3, PTHR15603:SF3, 1 hit |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF07653, SH3_2, 1 hit PF00791, ZU5, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 2 hits PS51145, ZU5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SH3B4_MOUSE | |
Accessioni | Q921I6Primary (citable) accession number: Q921I6 Secondary accession number(s): Q8BXV5, Q8BY95, Q8C007 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot