UniProtKB - Q921F2 (TADBP_MOUSE)
TAR DNA-binding protein 43
Tardbp
Functioni
GO - Molecular functioni
- double-stranded DNA binding Source: MGI
- identical protein binding Source: MGI
- mRNA 3'-UTR binding Source: MGI
- pre-mRNA intronic binding Source: MGI
- RNA binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- 3'-UTR-mediated mRNA destabilization Source: MGI
- 3'-UTR-mediated mRNA stabilization Source: MGI
- gene expression Source: MGI
- mRNA processing Source: UniProtKB-KW
- negative regulation by host of viral transcription Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- nuclear inner membrane organization Source: MGI
- positive regulation of protein import into nucleus Source: UniProtKB
- regulation of apoptotic process Source: MGI
- regulation of cell cycle Source: MGI
- regulation of circadian rhythm Source: UniProtKB
- regulation of protein stability Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- RNA splicing Source: MGI
Keywordsi
Molecular function | DNA-binding, Repressor, RNA-binding |
Biological process | Biological rhythms, mRNA processing, mRNA splicing, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: TAR DNA-binding protein 43Short name: TDP-43 |
Gene namesi | Name:Tardbp Synonyms:Tdp43 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2387629, Tardbp |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity1 Publication
- Stress granule By similarity
Note: Continuously travels in and out of the nucleus. Localizes to stress granules in response to oxidative stress.By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasmic stress granule Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081973 | 1 – 414 | TAR DNA-binding protein 43Add BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 79 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 84 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 95 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 102 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 181 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 183 | PhosphoserineCombined sources | 1 | |
Cross-linki | 263 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 292 | PhosphoserineBy similarity | 1 | |
Modified residuei | 293 | Omega-N-methylarginineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q921F2 |
jPOSTi | Q921F2 |
MaxQBi | Q921F2 |
PaxDbi | Q921F2 |
PRIDEi | Q921F2 |
TopDownProteomicsi | Q921F2 |
2D gel databases
REPRODUCTION-2DPAGEi | Q921F2 |
PTM databases
iPTMneti | Q921F2 |
PhosphoSitePlusi | Q921F2 |
SwissPalmi | Q921F2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041459, Expressed in presomitic mesoderm and 322 other tissues |
ExpressionAtlasi | Q921F2, baseline and differential |
Genevisiblei | Q921F2, MM |
Interactioni
Subunit structurei
Homodimer. Homooligomer (via its N-terminal domain) (By similarity).
Interacts with BRDT (PubMed:22570411). Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats.
Interacts with ATNX2; the interaction is RNA-dependent.
Interacts with MATR3.
Interacts with UBQLN2.
Interacts with HNRNPA2B1 (By similarity).
Interacts with ZNF106 (PubMed:28072389).
Interacts with CNOT7/CAF1 (By similarity).
Interacts with CRY2 (PubMed:27123980).
Interacts with PPIA/CYPA; the interaction is dependent on RNA-binding activity of TARDBP and PPIase activity of PPIA/CYPA (PubMed:25678563). Acetylation of PPIA/CYPA at 'Lys-125' favors the interaction of TARDBP with PPIA/CYPA (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 231054, 69 interactors |
CORUMi | Q921F2 |
IntActi | Q921F2, 12 interactors |
MINTi | Q921F2 |
STRINGi | 10090.ENSMUSP00000081142 |
Miscellaneous databases
RNActi | Q921F2, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q921F2 |
SMRi | Q921F2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q921F2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 200 | RRM 1PROSITE-ProRule annotationAdd BLAST | 97 | |
Domaini | 191 – 262 | RRM 2PROSITE-ProRule annotationAdd BLAST | 72 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 216 – 414 | Interaction with UBQLN2By similarityAdd BLAST | 199 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 274 – 413 | Gly-richAdd BLAST | 140 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QPQ8, Eukaryota |
GeneTreei | ENSGT00940000154343 |
HOGENOMi | CLU_012062_6_1_1 |
InParanoidi | Q921F2 |
OMAi | FCEIKRK |
OrthoDBi | 1425837at2759 |
TreeFami | TF315657 |
Family and domain databases
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR041105, TDP43_N |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits PF18694, TDP43_N, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 19 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC
60 70 80 90 100
MRGVRLVEGI LHAPDAGWGN LVYVVNYPKD NKRKMDETDA SSAVKVKRAV
110 120 130 140 150
QKTSDLIVLG LPWKTTEQDL KDYFSTFGEV LMVQVKKDLK TGHSKGFGFV
160 170 180 190 200
RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSPDEPLRS RKVFVGRCTE
210 220 230 240 250
DMTAEELQQF FCQYGEVVDV FIPKPFRAFA FVTFADDKVA QSLCGEDLII
260 270 280 290 300
KGISVHISNA EPKHNSNRQL ERSGRFGGNP GGFGNQGGFG NSRGGGAGLG
310 320 330 340 350
NNQGGNMGGG MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASQQNQSGPS
360 370 380 390 400
GNNQSQGSMQ REPNQAFGSG NNSYSGSNSG APLGWGSASN AGSGSGFNGG
410
FGSSMDSKSS GWGM
Computationally mapped potential isoform sequencesi
There are 19 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8BLD4 | Q8BLD4_MOUSE | TAR DNA-binding protein 43 | Tardbp TARDBP | 298 | Annotation score: | ||
Q8R0B4 | Q8R0B4_MOUSE | TAR DNA-binding protein 43 | Tardbp TARDBP | 295 | Annotation score: | ||
Q6VYI4 | Q6VYI4_MOUSE | TAR DNA-binding protein 43 | Tardbp TARDBP | 304 | Annotation score: | ||
Q6VYI5 | Q6VYI5_MOUSE | TAR DNA-binding protein 43 | Tardbp TARDBP | 302 | Annotation score: | ||
A0A087WR97 | A0A087WR97_MOUSE | TAR DNA-binding protein 43 | Tardbp | 243 | Annotation score: | ||
A0A087WRZ5 | A0A087WRZ5_MOUSE | TAR DNA-binding protein 43 | Tardbp | 285 | Annotation score: | ||
A0A087WSE4 | A0A087WSE4_MOUSE | TAR DNA-binding protein 43 | Tardbp | 149 | Annotation score: | ||
A0A087WP57 | A0A087WP57_MOUSE | TAR DNA-binding protein 43 | Tardbp | 84 | Annotation score: | ||
H3BJV1 | H3BJV1_MOUSE | TAR DNA-binding protein 43 | Tardbp | 140 | Annotation score: | ||
A0A087WQT4 | A0A087WQT4_MOUSE | TAR DNA-binding protein 43 | Tardbp | 35 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | T → K in BAE32189 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 96 | V → G in BAE32189 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 298 | G → C in BAE21557 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK133207 mRNA Translation: BAE21557.1 AK153803 mRNA Translation: BAE32189.1 BC012873 mRNA Translation: AAH12873.1 BC025544 mRNA Translation: AAH25544.1 BC027772 mRNA Translation: AAH27772.1 BC031126 mRNA Translation: AAH31126.1 BC033475 mRNA Translation: AAH33475.1 |
CCDSi | CCDS38971.1 |
RefSeqi | NP_001003899.1, NM_001003899.2 NP_001008545.1, NM_001008545.2 NP_001008546.1, NM_001008546.2 NP_001292354.1, NM_001305425.1 NP_663531.1, NM_145556.4 |
Genome annotation databases
Ensembli | ENSMUST00000084125; ENSMUSP00000081142; ENSMUSG00000041459 |
GeneIDi | 230908 |
KEGGi | mmu:230908 |
UCSCi | uc008vvg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK133207 mRNA Translation: BAE21557.1 AK153803 mRNA Translation: BAE32189.1 BC012873 mRNA Translation: AAH12873.1 BC025544 mRNA Translation: AAH25544.1 BC027772 mRNA Translation: AAH27772.1 BC031126 mRNA Translation: AAH31126.1 BC033475 mRNA Translation: AAH33475.1 |
CCDSi | CCDS38971.1 |
RefSeqi | NP_001003899.1, NM_001003899.2 NP_001008545.1, NM_001008545.2 NP_001008546.1, NM_001008546.2 NP_001292354.1, NM_001305425.1 NP_663531.1, NM_145556.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3D2W | X-ray | 1.65 | A | 192-265 | [»] | |
BMRBi | Q921F2 | |||||
SMRi | Q921F2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 231054, 69 interactors |
CORUMi | Q921F2 |
IntActi | Q921F2, 12 interactors |
MINTi | Q921F2 |
STRINGi | 10090.ENSMUSP00000081142 |
PTM databases
iPTMneti | Q921F2 |
PhosphoSitePlusi | Q921F2 |
SwissPalmi | Q921F2 |
2D gel databases
REPRODUCTION-2DPAGEi | Q921F2 |
Proteomic databases
EPDi | Q921F2 |
jPOSTi | Q921F2 |
MaxQBi | Q921F2 |
PaxDbi | Q921F2 |
PRIDEi | Q921F2 |
TopDownProteomicsi | Q921F2 |
Protocols and materials databases
Antibodypediai | 1854, 933 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084125; ENSMUSP00000081142; ENSMUSG00000041459 |
GeneIDi | 230908 |
KEGGi | mmu:230908 |
UCSCi | uc008vvg.1, mouse |
Organism-specific databases
CTDi | 23435 |
MGIi | MGI:2387629, Tardbp |
Phylogenomic databases
eggNOGi | ENOG502QPQ8, Eukaryota |
GeneTreei | ENSGT00940000154343 |
HOGENOMi | CLU_012062_6_1_1 |
InParanoidi | Q921F2 |
OMAi | FCEIKRK |
OrthoDBi | 1425837at2759 |
TreeFami | TF315657 |
Miscellaneous databases
BioGRID-ORCSi | 230908, 9 hits in 18 CRISPR screens |
ChiTaRSi | Tardbp, mouse |
EvolutionaryTracei | Q921F2 |
PROi | PR:Q921F2 |
RNActi | Q921F2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041459, Expressed in presomitic mesoderm and 322 other tissues |
ExpressionAtlasi | Q921F2, baseline and differential |
Genevisiblei | Q921F2, MM |
Family and domain databases
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR041105, TDP43_N |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits PF18694, TDP43_N, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TADBP_MOUSE | |
Accessioni | Q921F2Primary (citable) accession number: Q921F2 Secondary accession number(s): Q3U591, Q3V0E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 27, 2002 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references