Tardbp

Functionⁱ

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.By similarity

GO - Molecular functionⁱ

double-stranded DNA binding Source: MGI
identical protein binding Source: MGI
mRNA 3'-UTR binding Source: MGI
RNA binding Source: MGI
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 18836447

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

3'-UTR-mediated mRNA stabilization Source: MGI
mRNA processing Source: UniProtKB-KW
negative regulation by host of viral transcription Source: MGI
negative regulation of gene expression Source: MGI
negative regulation of protein phosphorylation Source: MGI
nuclear inner membrane organization Source: MGI
regulation of apoptotic process Source: MGI
regulation of cell cycle Source: MGI
RNA splicing Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor, RNA-binding
Biological process	mRNA processing, mRNA splicing, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: TAR DNA-binding protein 43 Short name: TDP-43
Gene namesⁱ	Name:Tardbp Synonyms:Tdp43
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:2387629 Tardbp

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000081973	1 – 414	TAR DNA-binding protein 43Add BLAST		414

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	79	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	84	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	95	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	102	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	181	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	183	PhosphoserineCombined sources	1
Cross-linkⁱ	263	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	292	PhosphoserineBy similarity	1
Modified residueⁱ	293	Omega-N-methylarginineCombined sources	1

Post-translational modificationⁱ

Hyperphosphorylated.By similarity

Ubiquitinated.By similarity

Keywords - PTMⁱ

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q921F2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q921F2
PaxDbⁱ	Q921F2
PRIDEⁱ	Q921F2
TopDownProteomicsⁱ	Q921F2

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	Q921F2

REPRODUCTION-2DPAGEⁱ

Q921F2

PTM databases

iPTMnetⁱ	Q921F2
PhosphoSitePlusⁱ	Q921F2
SwissPalmⁱ	Q921F2

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000041459
CleanExⁱ	MM_TARDBP
ExpressionAtlasⁱ	Q921F2 baseline and differential
Genevisibleⁱ	Q921F2 MM

Interactionⁱ

Subunit structureⁱ

Homodimer. Interacts with BRDT (PubMed:22570411). Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Interacts with MATR3. Interacts with UBQLN2. Interacts with HNRNPA2B1 (By similarity).By similarity1 Publication

GO - Molecular functionⁱ

identical protein binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	231054, 11 interactors
CORUMⁱ	Q921F2
IntActⁱ	Q921F2, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q921F2
STRINGⁱ	10090.ENSMUSP00000081142

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	192 – 197	Combined sources	6
Helixⁱ	204 – 211	Combined sources	8
Turnⁱ	212 – 214	Combined sources	3
Beta strandⁱ	217 – 221	Combined sources	5
Beta strandⁱ	228 – 235	Combined sources	8
Helixⁱ	237 – 243	Combined sources	7
Beta strandⁱ	247 – 250	Combined sources	4
Beta strandⁱ	253 – 259	Combined sources	7

3D structure databases

ProteinModelPortalⁱ	Q921F2
SMRⁱ	Q921F2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q921F2

EvolutionaryTraceⁱ

Q921F2

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	104 – 200	RRM 1PROSITE-ProRule annotationAdd BLAST		97
Domainⁱ	191 – 262	RRM 2PROSITE-ProRule annotationAdd BLAST		72

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	216 – 414	Interaction with UBQLN2By similarityAdd BLAST		199

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	274 – 413	Gly-richAdd BLAST		140

Domainⁱ

The RRM domains can bind to both DNA and RNA.

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410IFJS Eukaryota ENOG410ZYQJ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140835
HOGENOMⁱ	HOG000254792
HOVERGENⁱ	HBG058671
InParanoidⁱ	Q921F2
OMAⁱ	FCEIKRK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0BJZ
TreeFamⁱ	TF315657

Family and domain databases

Gene3Dⁱ	3.30.70.330, 2 hits
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00076 RRM_1, 2 hits
SMARTⁱ	View protein in SMART SM00360 RRM, 2 hits
SUPFAMⁱ	SSF54928 SSF54928, 2 hits
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Q921F2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC 
        60         70         80         90        100
MRGVRLVEGI LHAPDAGWGN LVYVVNYPKD NKRKMDETDA SSAVKVKRAV 
       110        120        130        140        150
QKTSDLIVLG LPWKTTEQDL KDYFSTFGEV LMVQVKKDLK TGHSKGFGFV 
       160        170        180        190        200
RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSPDEPLRS RKVFVGRCTE 
       210        220        230        240        250
DMTAEELQQF FCQYGEVVDV FIPKPFRAFA FVTFADDKVA QSLCGEDLII 
       260        270        280        290        300
KGISVHISNA EPKHNSNRQL ERSGRFGGNP GGFGNQGGFG NSRGGGAGLG 
       310        320        330        340        350
NNQGGNMGGG MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASQQNQSGPS 
       360        370        380        390        400
GNNQSQGSMQ REPNQAFGSG NNSYSGSNSG APLGWGSASN AGSGSGFNGG 
       410 
FGSSMDSKSS GWGM

Length:414

Mass (Da):44,548

Last modified:December 1, 2001 - v1

Checksum:ⁱ2BE6A695EC2CA106

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	8	T → K in BAE32189 (PubMed:16141072).Curated	1
Sequence conflictⁱ	96	V → G in BAE32189 (PubMed:16141072).Curated	1
Sequence conflictⁱ	298	G → C in BAE21557 (PubMed:16141072).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK133207 mRNA Translation: BAE21557.1 AK153803 mRNA Translation: BAE32189.1 BC012873 mRNA Translation: AAH12873.1 BC025544 mRNA Translation: AAH25544.1 BC027772 mRNA Translation: AAH27772.1 BC031126 mRNA Translation: AAH31126.1 BC033475 mRNA Translation: AAH33475.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS38971.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001003899.1, NM_001003899.2 NP_001008545.1, NM_001008545.2 NP_001008546.1, NM_001008546.2 NP_001292354.1, NM_001305425.1 NP_663531.1, NM_145556.4
UniGeneⁱ	Mm.22453 Mm.378962

Genome annotation databases

Ensemblⁱ	ENSMUST00000084125; ENSMUSP00000081142; ENSMUSG00000041459
GeneIDⁱ	230908
KEGGⁱ	mmu:230908
UCSC genome browser More...UCSCⁱ	uc008vvg.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q921F2	MCG16669, isoform CRA_b		MOUSE		414
TAR DNA-binding protein 43		CRIGR		213
TAR DNA-binding-like protein (Fragment)		Daphnia magna		128
UPI0003D77090		MOUSE		330
TAR DNA-binding protein 43 (Fragment)		MOUSE		149

Protein	Similar proteins		Species	Score	Length
Q921F2	TAR DNA-binding protein 43	)	HUMAN		414
TAR DNA binding protein		PANTR		414
TAR DNA-binding protein 43		PONAB		414
TAR DNA binding protein		PANPA		414
UPI0008F5070F		GORGO		253
+184

Protein	Similar proteins		Species	Score	Length
Q921F2	TAR DNA-binding protein 43	)	HUMAN		414
TAR DNA binding protein		PANTR		414
TAR DNA-binding protein 43		PONAB		414
TAR DNA binding protein		PANPA		414
UPI0008F5070F		GORGO		253
+367

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK133207 mRNA Translation: BAE21557.1 AK153803 mRNA Translation: BAE32189.1 BC012873 mRNA Translation: AAH12873.1 BC025544 mRNA Translation: AAH25544.1 BC027772 mRNA Translation: AAH27772.1 BC031126 mRNA Translation: AAH31126.1 BC033475 mRNA Translation: AAH33475.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS38971.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001003899.1, NM_001003899.2 NP_001008545.1, NM_001008545.2 NP_001008546.1, NM_001008546.2 NP_001292354.1, NM_001305425.1 NP_663531.1, NM_145556.4
UniGeneⁱ	Mm.22453 Mm.378962

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3D2W	X-ray	1.65	A	192-265	[»]
ProteinModelPortalⁱ	Q921F2
SMRⁱ	Q921F2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	231054, 11 interactors
CORUMⁱ	Q921F2
IntActⁱ	Q921F2, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q921F2
STRINGⁱ	10090.ENSMUSP00000081142

PTM databases

iPTMnetⁱ	Q921F2
PhosphoSitePlusⁱ	Q921F2
SwissPalmⁱ	Q921F2

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	Q921F2

REPRODUCTION-2DPAGEⁱ

Q921F2

Proteomic databases

EPDⁱ	Q921F2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q921F2
PaxDbⁱ	Q921F2
PRIDEⁱ	Q921F2
TopDownProteomicsⁱ	Q921F2

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000084125; ENSMUSP00000081142; ENSMUSG00000041459
GeneIDⁱ	230908
KEGGⁱ	mmu:230908
UCSC genome browser More...UCSCⁱ	uc008vvg.1 mouse

Organism-specific databases

CTDⁱ	23435
MGIⁱ	MGI:2387629 Tardbp

Phylogenomic databases

eggNOGⁱ	ENOG410IFJS Eukaryota ENOG410ZYQJ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140835
HOGENOMⁱ	HOG000254792
HOVERGENⁱ	HBG058671
InParanoidⁱ	Q921F2
OMAⁱ	FCEIKRK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0BJZ
TreeFamⁱ	TF315657

Miscellaneous databases

ChiTaRSⁱ	Tardbp mouse
EvolutionaryTraceⁱ	Q921F2
Protein Ontology More...PROⁱ	PR:Q921F2
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000041459
CleanExⁱ	MM_TARDBP
ExpressionAtlasⁱ	Q921F2 baseline and differential
Genevisibleⁱ	Q921F2 MM

Family and domain databases

Gene3Dⁱ	3.30.70.330, 2 hits
InterProⁱ	View protein in InterPro IPR012677 Nucleotide-bd_a/b_plait_sf IPR035979 RBD_domain_sf IPR000504 RRM_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00076 RRM_1, 2 hits
SMARTⁱ	View protein in SMART SM00360 RRM, 2 hits
SUPFAMⁱ	SSF54928 SSF54928, 2 hits
PROSITEⁱ	View protein in PROSITE PS50102 RRM, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TADBP_MOUSE
Accessionⁱ	Q921F2Primary (citable) accession number: Q921F2 Secondary accession number(s): Q3U591, Q3V0E7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
	Last sequence update:	December 1, 2001
	Last modified:	July 18, 2018
	This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q921F2 (TADBP_MOUSE)

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TAR DNA-binding protein 43

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ