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Protein

CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase

Gene

ST8SIA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei170SubstrateBy similarity1
Active sitei331Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL
  • sialic acid binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8 2681
ReactomeiR-HSA-4085001 Sialic acid metabolism
R-HSA-419037 NCAM1 interactions
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (EC:2.4.99.-)
Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name:
SIAT8-D
Sialyltransferase St8Sia IV
Short name:
ST8SiaIV
Gene namesi
Name:ST8SIA4
Synonyms:PST, PST1, SIAT8D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113532.12
HGNCiHGNC:10871 ST8SIA4
MIMi602547 gene
neXtProtiNX_Q92187

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 20Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST13
Topological domaini21 – 359LumenalSequence analysisAdd BLAST339

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi7903
OpenTargetsiENSG00000113532
PharmGKBiPA35772

Polymorphism and mutation databases

DMDMi2494834

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492931 – 359CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferaseAdd BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 2921 Publication
Disulfide bondi156 ↔ 3561 Publication
Glycosylationi204N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ92187
PaxDbiQ92187
PeptideAtlasiQ92187
PRIDEiQ92187
ProteomicsDBi75254

PTM databases

iPTMnetiQ92187
PhosphoSitePlusiQ92187

Expressioni

Tissue specificityi

Highly expressed in fetal brain, lung and kidney and in adult heart, spleen and thymus. Present to a lesser extent in adult brain, placenta, lung, large and small intestine and peripheral blood leukocytes.

Gene expression databases

BgeeiENSG00000113532 Expressed in 180 organ(s), highest expression level in frontal cortex
CleanExiHS_ST8SIA4
ExpressionAtlasiQ92187 baseline and differential
GenevisibleiQ92187 HS

Organism-specific databases

HPAiHPA051126
HPA053995

Interactioni

Protein-protein interaction databases

BioGridi113636, 78 interactors
STRINGi9606.ENSP00000231461

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ92187
SMRiQ92187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 193Substrate bindingBy similarity3
Regioni279 – 281Substrate bindingBy similarity3
Regioni314 – 315Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOGENOMiHOG000207604
HOVERGENiHBG056466
InParanoidiQ92187
KOiK06614
OMAiHSVEGWK
OrthoDBiEOG091G0AL4
PhylomeDBiQ92187
TreeFamiTF352820

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q92187-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELSLSRSLVN
60 70 80 90 100
SSDKIIRKAG SSIFQHNVEG WKINSSLVLE IRKNILRFLD AERDVSVVKS
110 120 130 140 150
SFKPGDVIHY VLDRRRTLNI SHDLHSLLPE VSPMKNRRFK TCAVVGNSGI
160 170 180 190 200
LLDSECGKEI DSHNFVIRCN LAPVVEFAAD VGTKSDFITM NPSVVQRAFG
210 220 230 240 250
GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV NALILKNKLK
260 270 280 290 300
VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF
310 320 330 340 350
WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK

LTTGKCVKQ
Length:359
Mass (Da):41,295
Last modified:February 1, 1997 - v1
Checksum:iC53FFDF658926D61
GO
Isoform 2 (identifier: Q92187-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-359: Missing.

Note: No experimental confirmation available.
Show »
Length:168
Mass (Da):19,260
Checksum:i2B9ED426B1A3C9F2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RFK3E5RFK3_HUMAN
CMP-N-acetylneuraminate-poly-alpha-...
ST8SIA4
46Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0689767R → K1 PublicationCorresponds to variant dbSNP:rs199768560Ensembl.1
Natural variantiVAR_03616992E → G in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044867169 – 359Missing in isoform 2. 1 PublicationAdd BLAST191

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41680 mRNA Translation: AAC41775.1
AK292872 mRNA Translation: BAF85561.1
AC010411 Genomic DNA No translation available.
AC117530 Genomic DNA No translation available.
CH471086 Genomic DNA Translation: EAW49104.1
CH471086 Genomic DNA Translation: EAW49105.1
BC027866 mRNA Translation: AAH27866.1
BC053657 mRNA Translation: AAH53657.1
CCDSiCCDS4091.1 [Q92187-1]
CCDS47252.1 [Q92187-2]
PIRiI59403
RefSeqiNP_005659.1, NM_005668.5 [Q92187-1]
NP_778222.1, NM_175052.2 [Q92187-2]
UniGeneiHs.308628

Genome annotation databases

EnsembliENST00000231461; ENSP00000231461; ENSG00000113532 [Q92187-1]
ENST00000451528; ENSP00000428914; ENSG00000113532 [Q92187-2]
GeneIDi7903
KEGGihsa:7903
UCSCiuc003knk.5 human [Q92187-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia IV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41680 mRNA Translation: AAC41775.1
AK292872 mRNA Translation: BAF85561.1
AC010411 Genomic DNA No translation available.
AC117530 Genomic DNA No translation available.
CH471086 Genomic DNA Translation: EAW49104.1
CH471086 Genomic DNA Translation: EAW49105.1
BC027866 mRNA Translation: AAH27866.1
BC053657 mRNA Translation: AAH53657.1
CCDSiCCDS4091.1 [Q92187-1]
CCDS47252.1 [Q92187-2]
PIRiI59403
RefSeqiNP_005659.1, NM_005668.5 [Q92187-1]
NP_778222.1, NM_175052.2 [Q92187-2]
UniGeneiHs.308628

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5Y22NMR-A258-279[»]
5Y3UNMR-A258-279[»]
ProteinModelPortaliQ92187
SMRiQ92187
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113636, 78 interactors
STRINGi9606.ENSP00000231461

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

PTM databases

iPTMnetiQ92187
PhosphoSitePlusiQ92187

Polymorphism and mutation databases

DMDMi2494834

Proteomic databases

EPDiQ92187
PaxDbiQ92187
PeptideAtlasiQ92187
PRIDEiQ92187
ProteomicsDBi75254

Protocols and materials databases

DNASUi7903
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000231461; ENSP00000231461; ENSG00000113532 [Q92187-1]
ENST00000451528; ENSP00000428914; ENSG00000113532 [Q92187-2]
GeneIDi7903
KEGGihsa:7903
UCSCiuc003knk.5 human [Q92187-1]

Organism-specific databases

CTDi7903
DisGeNETi7903
EuPathDBiHostDB:ENSG00000113532.12
GeneCardsiST8SIA4
HGNCiHGNC:10871 ST8SIA4
HPAiHPA051126
HPA053995
MIMi602547 gene
neXtProtiNX_Q92187
OpenTargetsiENSG00000113532
PharmGKBiPA35772
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOGENOMiHOG000207604
HOVERGENiHBG056466
InParanoidiQ92187
KOiK06614
OMAiHSVEGWK
OrthoDBiEOG091G0AL4
PhylomeDBiQ92187
TreeFamiTF352820

Enzyme and pathway databases

UniPathwayi
UPA00378

BRENDAi2.4.99.8 2681
ReactomeiR-HSA-4085001 Sialic acid metabolism
R-HSA-419037 NCAM1 interactions

Miscellaneous databases

ChiTaRSiST8SIA4 human
GeneWikiiST8SIA4
GenomeRNAii7903
PROiPR:Q92187
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113532 Expressed in 180 organ(s), highest expression level in frontal cortex
CleanExiHS_ST8SIA4
ExpressionAtlasiQ92187 baseline and differential
GenevisibleiQ92187 HS

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8D_HUMAN
AccessioniPrimary (citable) accession number: Q92187
Secondary accession number(s): A8KA07
, G3V104, Q8N1F4, Q92693
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 10, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  7. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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