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Protein

Pyruvate kinase PKM

Gene

pkm

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May modulate the metamorphic process by regulating the level of intracellular thyroid hormones.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (XELAEV_18036725mg), Glyceraldehyde-3-phosphate dehydrogenase (gapdh), Glyceraldehyde-3-phosphate dehydrogenase (gapdh)
  2. Phosphoglycerate kinase (XELAEV_18042225mg), Phosphoglycerate kinase (pgk1), Phosphoglycerate kinase
  3. no protein annotated in this organism
  4. Enolase 4 (eno4), Alpha-enolase (eno1)
  5. Pyruvate kinase, Pyruvate kinase (XELAEV_18018020mg), Pyruvate kinase (pklr), Pyruvate kinase (XELAEV_18040950mg), Pyruvate kinase (XELAEV_18018020mg), Pyruvate kinase PKM (pkm), Pyruvate kinase (XELAEV_18020793mg), Pyruvate kinase (pkm), Pyruvate kinase (XELAEV_18043021mg), Pyruvate kinase (LOC100037030)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69SubstrateBy similarity1
Metal bindingi71PotassiumBy similarity1
Metal bindingi73PotassiumBy similarity1
Metal bindingi109PotassiumBy similarity1
Metal bindingi110Potassium; via carbonyl oxygenBy similarity1
Sitei266Transition state stabilizerBy similarity1
Metal bindingi268MagnesiumSequence analysis1
Binding sitei291Substrate; via amide nitrogenBy similarity1
Metal bindingi292MagnesiumBy similarity1
Binding sitei292Substrate; via amide nitrogenBy similarity1
Binding sitei324SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKM (EC:2.7.1.40)
Alternative name(s):
Cytosolic thyroid hormone-binding protein
Short name:
CTHBP
Gene namesi
Name:pkm
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-485877 pkm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120981 – 527Pyruvate kinase PKMAdd BLAST527

Proteomic databases

PRIDEiQ92122

Expressioni

Developmental stagei

Expressed in all stages of tadpole development. In the tail, high levels found in the premetamorphic stage (levels 54-60). Levels decrease with tail resorption. In the hindlimb, low expression during limb morphogenesis (stages 54-56), increased levels with limb growth (stages 58-66). Levels increase only slightly during intestine remodeling.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi100773, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ92122
SMRiQ92122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOVERGENiHBG000941
KOiK00873

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q92122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAGSAFIQ TQQLHAAMAD TFLEHMCRLD IDSEPIVARN TGIICTIGPA
60 70 80 90 100
SRSVEMLKEM IKSGMNIARL NFSHGTHEYH AGTIKNVREA TESLASNPIH
110 120 130 140 150
YRPVAVALDT KGPEIRTGLI KGSGTAEVEL KKGATMRITL DDAFQENCDE
160 170 180 190 200
NVLWVDYKNL TKVVKPGSKI YVDDGLISLL VKEIGPDFCV TEIENGGMLG
210 220 230 240 250
SKKGVNLPGA AVDLPAVSSK DIQDLQFGVE QDVDMVFASF IRKAADVHEV
260 270 280 290 300
REVLGEKGKN IKIISKIENH EGVRRFDEIL EASDGIMVAR GDLGIEIPAE
310 320 330 340 350
KVFLAQKMMI GRCNRAGKPV ICATQMLESM IKKPRPTRAE GSDVANAVLD
360 370 380 390 400
GADCIMLSGE TAKGDYPLEA VRMQHAIARE AEAAIFHRQL FEELRRVSPL
410 420 430 440 450
TRDPTEATAV GAVEASFKCS SGAIIVLTKS GRSAHLLSRY RPRAPIISVT
460 470 480 490 500
RNGQTARQAH LYRGIFPVLY REAVHEAWAE DVDSRVNFAM DIGKARGFFK
510 520
SGDVVIVLTG WRPGSGFTNT MRVVPVP
Length:527
Mass (Da):57,525
Last modified:November 1, 1996 - v1
Checksum:iA8379F0E65325A66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03878 mRNA Translation: AAA63581.1
BC079921 mRNA Translation: AAH79921.1
RefSeqiNP_001084341.1, NM_001090872.1
UniGeneiXl.21936

Genome annotation databases

GeneIDi399448
KEGGixla:399448

Similar proteinsi

Entry informationi

Entry nameiKPYM_XENLA
AccessioniPrimary (citable) accession number: Q92122
Secondary accession number(s): Q68FC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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