UniProtKB - Q920V1 (B3GL1_MOUSE)
Protein
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
Gene
B3galnt1
Organism
Mus musculus (Mouse)
Status
Functioni
Transfers N-acetylgalactosamine onto globotriaosylceramide (PubMed:9417047). Plays a critical role in preimplantation stage embryonic development (PubMed:11463849).2 Publications
Catalytic activityi
- globoside Gb3Cer (d18:1(4E)) + UDP-N-acetyl-α-D-galactosamine = globoside Gb4Cer (d18:1(4E)) + H+ + UDPBy similarityEC:2.4.1.79By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- acetylgalactosaminyltransferase activity Source: GO_Central
- acetylglucosaminyltransferase activity Source: GO_Central
- galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
- N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: GO_Central
- transferase activity, transferring hexosyl groups Source: GO_Central
- UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Source: MGI
GO - Biological processi
- oligosaccharide biosynthetic process Source: MGI
- poly-N-acetyllactosamine biosynthetic process Source: GO_Central
- protein glycosylation Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Magnesium |
Enzyme and pathway databases
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.79By similarity)Short name: Beta-1,3-GalNAc-T1 Alternative name(s): Beta-1,3-galactosyltransferase 3 Short name: Beta-1,3-GalTase 3 Short name: Beta3Gal-T3 Short name: Beta3GalT3 Short name: b3Gal-T3 Beta-3-Gx-T3 Brainiac 11 Publication Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase Globoside synthase UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349405, B3galnt1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 21 – 43 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 44 – 331 | LumenalSequence analysisAdd BLAST | 288 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Embryonic lethal. Lethality occurs at the preimplantation stage, between 3.5 and 4.5 days post-coitum (dpc).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219154 | 1 – 331 | UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1Add BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 72 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 154 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 198 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 212 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 326 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q920V1 |
PaxDbi | Q920V1 |
PeptideAtlasi | Q920V1 |
PRIDEi | Q920V1 |
PTM databases
GlyConnecti | 2808, 1 N-Linked glycan (1 site) |
GlyGeni | Q920V1, 5 sites |
PhosphoSitePlusi | Q920V1 |
Expressioni
Tissue specificityi
Detected in brain, ovary, kidney, uterus and stomach (PubMed:9417047, PubMed:11463849). In ovary, specifically expressed in follicular granulosa cells and shows particularly strong expression at later stages of follicle development (PubMed:11463849).2 Publications
Developmental stagei
Detected in embryos from 12.5 days post coitum (dpc) onwards. Expressed in all four ventricles of the developing brain, with highest expression in the outer ventricular layer. Also found in the limb buds at 12.5 dpc. Detected in the brain (hippocampus) and retina at postnatal day 1. Expression in the retina is localized to the ganglion cell layer. At postnatal day 10, expression remains strong in the hippocampus where it localizes to the four CA fields and the dentate gyrus.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000043300, Expressed in decidua and 294 other tissues |
Genevisiblei | Q920V1, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000058363 |
Miscellaneous databases
RNActi | Q920V1, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 31 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000162252 |
HOGENOMi | CLU_036849_2_4_1 |
InParanoidi | Q920V1 |
OMAi | YRQDFRF |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q920V1 |
TreeFami | TF318639 |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
i Sequence
Sequence statusi: Complete.
Q920V1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPAVLTALP NRMSLRSLKW SLLLLSLLSF LVIWYLSLPH YNVIERVNWM
60 70 80 90 100
YFYEYEPIYR QDFRFTLREH SNCSHQNPFL VILVTSRPSD VKARQAIRVT
110 120 130 140 150
WGEKKSWWGY EVLTFFLLGQ QAEREDKTLA LSLEDEHVLY GDIIRQDFLD
160 170 180 190 200
TYNNLTLKTI MAFRWVMEFC PNAKYIMKTD TDVFINTGNL VKYLLNLNHS
210 220 230 240 250
EKFFTGYPLI DNYSYRGFFH KNHISYQEYP FKVFPPYCSG LGYIMSGDLV
260 270 280 290 300
PRVYEMMSHV KPIKFEDVYV GICLNLLKVD IHIPEDTNLF FLYRIHLDVC
310 320 330
QLRRVIAAHG FSSKEIITFW QVMLRNTTCH Y
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 4 | A → T in strain: NJL/Msf, BLG2/Msf, MSM/Msf and SWN/Msf. 1 Publication | 1 | |
Natural varianti | 87 | R → H in strain: SWN/Msf. 1 Publication | 1 | |
Natural varianti | 128 | T → M in strain: CAST/Ei. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB039154 Genomic DNA Translation: BAB68678.1 AB039155 Genomic DNA Translation: BAB68679.1 AB039156 Genomic DNA Translation: BAB68680.1 AB039157 Genomic DNA Translation: BAB68681.1 AB039158 Genomic DNA Translation: BAB68682.1 AB039159 Genomic DNA Translation: BAB68683.1 AB039160 Genomic DNA Translation: BAB68684.1 AB039161 Genomic DNA Translation: BAB68685.1 AB039162 Genomic DNA Translation: BAB68686.1 AF029792 Genomic DNA Translation: AAC53525.1 AK003837 mRNA Translation: BAB23028.1 AK088407 mRNA Translation: BAC40336.1 BC003835 mRNA Translation: AAH03835.3 |
CCDSi | CCDS17406.1 |
RefSeqi | NP_064410.1, NM_020026.4 XP_006501592.1, XM_006501529.3 |
Genome annotation databases
Ensembli | ENSMUST00000061826; ENSMUSP00000058363; ENSMUSG00000043300 |
GeneIDi | 26879 |
KEGGi | mmu:26879 |
UCSCi | uc008pmj.2, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase b3GalT3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB039154 Genomic DNA Translation: BAB68678.1 AB039155 Genomic DNA Translation: BAB68679.1 AB039156 Genomic DNA Translation: BAB68680.1 AB039157 Genomic DNA Translation: BAB68681.1 AB039158 Genomic DNA Translation: BAB68682.1 AB039159 Genomic DNA Translation: BAB68683.1 AB039160 Genomic DNA Translation: BAB68684.1 AB039161 Genomic DNA Translation: BAB68685.1 AB039162 Genomic DNA Translation: BAB68686.1 AF029792 Genomic DNA Translation: AAC53525.1 AK003837 mRNA Translation: BAB23028.1 AK088407 mRNA Translation: BAC40336.1 BC003835 mRNA Translation: AAH03835.3 |
CCDSi | CCDS17406.1 |
RefSeqi | NP_064410.1, NM_020026.4 XP_006501592.1, XM_006501529.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000058363 |
Protein family/group databases
CAZyi | GT31, Glycosyltransferase Family 31 |
PTM databases
GlyConnecti | 2808, 1 N-Linked glycan (1 site) |
GlyGeni | Q920V1, 5 sites |
PhosphoSitePlusi | Q920V1 |
Proteomic databases
MaxQBi | Q920V1 |
PaxDbi | Q920V1 |
PeptideAtlasi | Q920V1 |
PRIDEi | Q920V1 |
Protocols and materials databases
Antibodypediai | 33673, 93 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000061826; ENSMUSP00000058363; ENSMUSG00000043300 |
GeneIDi | 26879 |
KEGGi | mmu:26879 |
UCSCi | uc008pmj.2, mouse |
Organism-specific databases
CTDi | 8706 |
MGIi | MGI:1349405, B3galnt1 |
Phylogenomic databases
eggNOGi | KOG2287, Eukaryota |
GeneTreei | ENSGT00940000162252 |
HOGENOMi | CLU_036849_2_4_1 |
InParanoidi | Q920V1 |
OMAi | YRQDFRF |
OrthoDBi | 1037602at2759 |
PhylomeDBi | Q920V1 |
TreeFami | TF318639 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-MMU-1660662, Glycosphingolipid metabolism |
Miscellaneous databases
BioGRID-ORCSi | 26879, 1 hit in 18 CRISPR screens |
ChiTaRSi | B3galnt1, mouse |
PROi | PR:Q920V1 |
RNActi | Q920V1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000043300, Expressed in decidua and 294 other tissues |
Genevisiblei | Q920V1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR002659, Glyco_trans_31 |
PANTHERi | PTHR11214, PTHR11214, 1 hit |
Pfami | View protein in Pfam PF01762, Galactosyl_T, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B3GL1_MOUSE | |
Accessioni | Q920V1Primary (citable) accession number: Q920V1 Secondary accession number(s): O54906 Q9CTE5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 10, 2021 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families