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Protein

DNA repair protein REV1

Gene

Rev1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei355dCTPBy similarity1
Metal bindingi421Magnesium 1PROSITE-ProRule annotation1
Metal bindingi421Magnesium 2PROSITE-ProRule annotation1
Binding sitei520dCTPBy similarity1
Metal bindingi568Magnesium 1PROSITE-ProRule annotation1
Binding sitei568dCTPBy similarity1
Metal bindingi569Magnesium 1PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi421 – 425dCTP bindingBy similarity5
Nucleotide bindingi508 – 514dCTP bindingBy similarity7

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • deoxycytidyl transferase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-110312 Translesion synthesis by REV1
R-MMU-5655862 Translesion synthesis by POLK
R-MMU-5656121 Translesion synthesis by POLI
R-MMU-5656169 Termination of translesion DNA synthesis

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein REV1 (EC:2.7.7.-)
Alternative name(s):
Rev1-like terminal deoxycytidyl transferase
Gene namesi
Name:Rev1
Synonyms:Rev1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1929074 Rev1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739931 – 1249DNA repair protein REV1Add BLAST1249

Proteomic databases

PaxDbiQ920Q2
PeptideAtlasiQ920Q2
PRIDEiQ920Q2

PTM databases

iPTMnetiQ920Q2
PhosphoSitePlusiQ920Q2

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000026082 Expressed in 270 organ(s), highest expression level in testis
CleanExiMM_REV1
ExpressionAtlasiQ920Q2 baseline and differential
GenevisibleiQ920Q2 MM

Interactioni

Subunit structurei

Interacts with FAAP20 (By similarity). Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei768Interaction with target DNABy similarity1
Sitei781Interaction with target DNABy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
FAAP20Q6NZ36-17EBI-2114764,EBI-15965017From a different organism.

Protein-protein interaction databases

BioGridi207845, 10 interactors
DIPiDIP-41772N
IntActiQ920Q2, 5 interactors
STRINGi10090.ENSMUSP00000027251

Structurei

Secondary structure

11249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ920Q2
SMRiQ920Q2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 131BRCTPROSITE-ProRule annotationAdd BLAST88
Domaini417 – 651UmuCPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni350 – 360Interaction with target DNABy similarityAdd BLAST11
Regioni651 – 654Interaction with target DNABy similarity4
Regioni707 – 715Interaction with target DNABy similarity9
Regioni1150 – 1249Protein interaction domain; mediates interaction with DNA polymerase zetaAdd BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1072 – 1078Nuclear localization signalSequence analysis7

Domaini

The C-terminal domain is necessary for protein interactions.

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

eggNOGiKOG2093 Eukaryota
COG0389 LUCA
GeneTreeiENSGT00530000062942
HOGENOMiHOG000154094
HOVERGENiHBG054140
InParanoidiQ920Q2
KOiK03515
OMAiNMAFDFI
OrthoDBiEOG091G02UI
PhylomeDBiQ920Q2
TreeFamiTF314488

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di1.20.58.1280, 1 hit
3.30.1490.100, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR025527 DUF4414
IPR012112 REV1
IPR031991 Rev1_C
IPR038401 Rev1_C_sf
IPR001126 UmuC
PfamiView protein in Pfam
PF16589 BRCT_2, 1 hit
PF14377 DUF4414, 1 hit
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF16727 REV1_C, 1 hit
PIRSFiPIRSF036573 REV1, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS50173 UMUC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q920Q2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRRGGWRKRT ENDGWEKWGG YMAAKVQKLE EQFRTDAANQ KDGTASAIFS
60 70 80 90 100
GVAIYVNGYT DPSAEELRNL MMLHGGQYHV YYSRSKTTHI IATNLPNAKI
110 120 130 140 150
KELKGEKVIR PEWIVESIKA GRLLSSAPYQ LYTKPSAAQK SLNFNPVCKP
160 170 180 190 200
EDPGPGPSNR AKQLNNRVNH IIKKIETESE VKANGLSSWN EDGVNDDFSF
210 220 230 240 250
EDLEHTFPGR KQNGVMHPRD TAVIFNGHTH SSNGALKTQD CLVPVGNSVA
260 270 280 290 300
SRLSLDSTQE EKRAEKSNAD FRDCTVQHLQ HSTRSADALR SPHRTNSLSP
310 320 330 340 350
SLHSNTKING AHHSTVQGPS STKSTSVLTL SKVAPSVPSK PSDCNFISDF
360 370 380 390 400
YSRSRLHHIS TWKCELTEFV NTLQRQSSGI FPGREKLKKV KTGRSSLVVT
410 420 430 440 450
DTGTMSVLSS PRHQSCVMHV DMDCFFVSVG IRNRPDLKGK PVAVTSNRGT
460 470 480 490 500
GTAPLRPGAN PQLEWQYYQN RALRGKAADI PDSSVWENQD STQTNGIDSV
510 520 530 540 550
LSKAEIASCS YEARQVGIKN GMFFGYAKQL CPNLQAVPYD FHACREVAQA
560 570 580 590 600
MYETLASYTH SIEAVSCDEA LIDVTDILAE TKLSPEEFAA ALRIEIKDKT
610 620 630 640 650
KCAASVGIGS NILLARMATK KAKPDGQYHL QPDEVDDFIR GQLVTNLPGV
660 670 680 690 700
GRSMESKLAS LGIKTCGDLQ CLTMAKLQKE FGPKTGQMLY RFCRGLDDRP
710 720 730 740 750
VRTEKERKSV SAEINYGIRF TQPKEAEAFL LSLSEEIQRR LEAAGMKGKR
760 770 780 790 800
LTLKIMVRKP GAPIETAKFG GHGICDNIAR TVTLDQATDS AKIIGKATLN
810 820 830 840 850
MFHTMKLNIS DMRGVGIQVN QLVPANSNLS TCSSRPSAQS SLFSGRPHSV
860 870 880 890 900
RDLFQLQKAK KPTEEEHKEV FLAAVDLEVS STSRACGLLS PLSAHLAASV
910 920 930 940 950
SPDTNSGECS RKWNGLHSPV SGQSRLNLSI EVPSPSQIDQ SVLEALPLDL
960 970 980 990 1000
REQIEQVCAA QQGEPRGKKK EPVNGCSSGV LPHPVGTVLL QIPEPQEPCN
1010 1020 1030 1040 1050
SDSKISVIAL PAFSQVDPDV FAALPAELQK ELKAAYDQRQ RQGEDTTHQQ
1060 1070 1080 1090 1100
PTSTSVPKNP LLQLKPPAMK DKRNKRKNLI GSPRKSPLKN KLLSSPAKTL
1110 1120 1130 1140 1150
PGAYGSPQKL MDGFLQHEGM ASERPLEEVS ASTPGAQDLS SLLPGQSSCF
1160 1170 1180 1190 1200
RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD
1210 1220 1230 1240
LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT
Length:1,249
Mass (Da):137,341
Last modified:December 1, 2001 - v1
Checksum:iF82E0B6171AA3F75
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YW35A0A0A6YW35_MOUSE
DNA repair protein REV1
Rev1
138Annotation score:
A0A0A6YWI0A0A0A6YWI0_MOUSE
DNA repair protein REV1
Rev1
98Annotation score:
A0A0A6YX77A0A0A6YX77_MOUSE
DNA repair protein REV1
Rev1
82Annotation score:
A0A0A6YWQ5A0A0A6YWQ5_MOUSE
DNA repair protein REV1
Rev1
22Annotation score:
A0A0A6YX32A0A0A6YX32_MOUSE
DNA repair protein REV1
Rev1
93Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti438K → T in AAF23323 (Ref. 2) Curated1
Sequence conflicti555L → W in AAF23323 (Ref. 2) Curated1
Sequence conflicti638F → S in AAF23323 (Ref. 2) Curated1
Sequence conflicti661L → S in AAF23323 (Ref. 2) Curated1
Sequence conflicti684K → R in AAF23323 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057418 mRNA Translation: BAB64933.1
AF179302 mRNA Translation: AAF23323.1
BC058093 mRNA Translation: AAH58093.1
CCDSiCCDS14899.1
RefSeqiNP_062516.2, NM_019570.3
XP_006496221.1, XM_006496158.2
UniGeneiMm.389103

Genome annotation databases

EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082
GeneIDi56210
KEGGimmu:56210
UCSCiuc007asp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057418 mRNA Translation: BAB64933.1
AF179302 mRNA Translation: AAF23323.1
BC058093 mRNA Translation: AAH58093.1
CCDSiCCDS14899.1
RefSeqiNP_062516.2, NM_019570.3
XP_006496221.1, XM_006496158.2
UniGeneiMm.389103

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LSGNMR-A1150-1249[»]
2LSJNMR-A1135-1249[»]
4FJOX-ray2.72A1153-1249[»]
ProteinModelPortaliQ920Q2
SMRiQ920Q2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207845, 10 interactors
DIPiDIP-41772N
IntActiQ920Q2, 5 interactors
STRINGi10090.ENSMUSP00000027251

PTM databases

iPTMnetiQ920Q2
PhosphoSitePlusiQ920Q2

Proteomic databases

PaxDbiQ920Q2
PeptideAtlasiQ920Q2
PRIDEiQ920Q2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082
GeneIDi56210
KEGGimmu:56210
UCSCiuc007asp.1 mouse

Organism-specific databases

CTDi51455
MGIiMGI:1929074 Rev1

Phylogenomic databases

eggNOGiKOG2093 Eukaryota
COG0389 LUCA
GeneTreeiENSGT00530000062942
HOGENOMiHOG000154094
HOVERGENiHBG054140
InParanoidiQ920Q2
KOiK03515
OMAiNMAFDFI
OrthoDBiEOG091G02UI
PhylomeDBiQ920Q2
TreeFamiTF314488

Enzyme and pathway databases

ReactomeiR-MMU-110312 Translesion synthesis by REV1
R-MMU-5655862 Translesion synthesis by POLK
R-MMU-5656121 Translesion synthesis by POLI
R-MMU-5656169 Termination of translesion DNA synthesis

Miscellaneous databases

ChiTaRSiRev1 mouse
PROiPR:Q920Q2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026082 Expressed in 270 organ(s), highest expression level in testis
CleanExiMM_REV1
ExpressionAtlasiQ920Q2 baseline and differential
GenevisibleiQ920Q2 MM

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di1.20.58.1280, 1 hit
3.30.1490.100, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR025527 DUF4414
IPR012112 REV1
IPR031991 Rev1_C
IPR038401 Rev1_C_sf
IPR001126 UmuC
PfamiView protein in Pfam
PF16589 BRCT_2, 1 hit
PF14377 DUF4414, 1 hit
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF16727 REV1_C, 1 hit
PIRSFiPIRSF036573 REV1, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS50173 UMUC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiREV1_MOUSE
AccessioniPrimary (citable) accession number: Q920Q2
Secondary accession number(s): Q9QXV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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