UniProtKB - Q920P5 (KAD5_MOUSE)
Protein
Adenylate kinase isoenzyme 5
Gene
Ak5
Organism
Mus musculus (Mouse)
Status
Functioni
Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.By similarity
Catalytic activityi
- EC:2.7.4.3By similarity
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADPBy similarityEC:2.7.4.6By similarity
- EC:2.7.4.6By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 168 | AMP 1By similarity | 1 | |
Binding sitei | 226 | AMP 1By similarity | 1 | |
Binding sitei | 257 | ATP 1By similarity | 1 | |
Binding sitei | 263 | AMP 1By similarity | 1 | |
Binding sitei | 274 | AMP 1By similarity | 1 | |
Binding sitei | 407 | AMP 2By similarity | 1 | |
Binding sitei | 412 | AMP 2By similarity | 1 | |
Binding sitei | 469 | AMP 2By similarity | 1 | |
Binding sitei | 500 | ATP 2By similarity | 1 | |
Binding sitei | 506 | AMP 2By similarity | 1 | |
Binding sitei | 517 | AMP 2By similarity | 1 | |
Binding sitei | 545 | ATP 2; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 142 – 147 | ATP 1By similarity | 6 | |
Nucleotide bindingi | 191 – 193 | AMP 1By similarity | 3 | |
Nucleotide bindingi | 219 – 222 | AMP 1By similarity | 4 | |
Nucleotide bindingi | 386 – 391 | ATP 2By similarity | 6 | |
Nucleotide bindingi | 433 – 435 | AMP 2By similarity | 3 | |
Nucleotide bindingi | 462 – 465 | AMP 2By similarity | 4 |
GO - Molecular functioni
- adenylate kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- nucleoside diphosphate kinase activity Source: UniProtKB
GO - Biological processi
- ATP metabolic process Source: InterPro
- nucleoside diphosphate phosphorylation Source: UniProtKB
- nucleoside triphosphate biosynthetic process Source: UniProtKB
- purine nucleotide metabolic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-499943, Interconversion of nucleotide di- and triphosphates |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ak5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2677491, Ak5 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytoskeleton
- centriolar satellite Source: MGI
Cytosol
- cytosol Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000158931 | 1 – 562 | Adenylate kinase isoenzyme 5Add BLAST | 562 |
Proteomic databases
PaxDbi | Q920P5 |
PeptideAtlasi | Q920P5 |
PRIDEi | Q920P5 |
PTM databases
iPTMneti | Q920P5 |
PhosphoSitePlusi | Q920P5 |
Expressioni
Tissue specificityi
Brain specific.By similarity
Gene expression databases
Bgeei | ENSMUSG00000039058, Expressed in dentate gyrus of hippocampal formation granule cell and 183 other tissues |
Genevisiblei | Q920P5, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 230921, 5 interactors |
IntActi | Q920P5, 2 interactors |
STRINGi | 10090.ENSMUSP00000042785 |
Miscellaneous databases
RNActi | Q920P5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 133 – 316 | Adenylate kinase 1By similarityAdd BLAST | 184 | |
Regioni | 162 – 193 | NMPbind 1By similarityAdd BLAST | 32 | |
Regioni | 256 – 266 | LID 1By similarityAdd BLAST | 11 | |
Regioni | 377 – 559 | Adenylate kinase 2By similarityAdd BLAST | 183 | |
Regioni | 406 – 435 | NMPbind 2By similarityAdd BLAST | 30 | |
Regioni | 499 – 509 | LID 2By similarityAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the adenylate kinase family.Curated
Phylogenomic databases
eggNOGi | KOG3079, Eukaryota |
GeneTreei | ENSGT00940000155917 |
HOGENOMi | CLU_034712_1_0_1 |
InParanoidi | Q920P5 |
OrthoDBi | 1378291at2759 |
PhylomeDBi | Q920P5 |
TreeFami | TF313747 |
Family and domain databases
CDDi | cd01428, ADK, 2 hits |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 2 hits |
InterProi | View protein in InterPro IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR006267, AK1/5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR23359, PTHR23359, 3 hits |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR01360, aden_kin_iso1, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q920P5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNTNDAKEYL ARRDIPQLFE SLLNGLMCSK PEDPIEYLET CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSASSNRKW SLIAKIITNG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLQKRAEQ QGRPDDNLKA TQRRLVNFKQ NAAPLVKYFQ EKGLIVTFDA
310 320 330 340 350
DRDEDAVFHD ISVAVDSKLF PNKEAPMDSS DLDPSMMFDA GEIIDTGSDY
360 370 380 390 400
DNQDDDQLNV FGEDTEGGFM EDLRKCKIIF LMGGPGSGKG TQCEKLAEKY
410 420 430 440 450
GFTHLSTGEL LRQELTSESE RSKLIRDIME RGDLVPSGVV LELLKEAMVA
460 470 480 490 500
SLGNTKGFLI DGYPREVKQG EEFGRRIGDP HLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SQSSQRGEDG AKSIAKRLEA YHRASIPVVT YYERKTQLRK VNAEGTPEQV
560
FLQLCTAIDS VF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JGN7 | A0A0G2JGN7_MOUSE | Nucleoside-diphosphate kinase | Ak5 | 201 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 249 | R → K in AK053807 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037880 | 1 – 369 | Missing in isoform 2. 1 PublicationAdd BLAST | 369 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB060081 mRNA Translation: BAB69859.1 AK053807 mRNA No translation available. AC111139 Genomic DNA No translation available. AC116720 Genomic DNA No translation available. |
CCDSi | CCDS38675.1 [Q920P5-1] |
RefSeqi | NP_001074746.1, NM_001081277.1 [Q920P5-1] |
Genome annotation databases
Ensembli | ENSMUST00000045262; ENSMUSP00000042785; ENSMUSG00000039058 [Q920P5-1] |
GeneIDi | 229949 |
KEGGi | mmu:229949 |
UCSCi | uc008rtq.1, mouse [Q920P5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB060081 mRNA Translation: BAB69859.1 AK053807 mRNA No translation available. AC111139 Genomic DNA No translation available. AC116720 Genomic DNA No translation available. |
CCDSi | CCDS38675.1 [Q920P5-1] |
RefSeqi | NP_001074746.1, NM_001081277.1 [Q920P5-1] |
3D structure databases
SMRi | Q920P5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230921, 5 interactors |
IntActi | Q920P5, 2 interactors |
STRINGi | 10090.ENSMUSP00000042785 |
PTM databases
iPTMneti | Q920P5 |
PhosphoSitePlusi | Q920P5 |
Proteomic databases
PaxDbi | Q920P5 |
PeptideAtlasi | Q920P5 |
PRIDEi | Q920P5 |
Protocols and materials databases
Antibodypediai | 19726, 345 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000045262; ENSMUSP00000042785; ENSMUSG00000039058 [Q920P5-1] |
GeneIDi | 229949 |
KEGGi | mmu:229949 |
UCSCi | uc008rtq.1, mouse [Q920P5-1] |
Organism-specific databases
CTDi | 26289 |
MGIi | MGI:2677491, Ak5 |
Phylogenomic databases
eggNOGi | KOG3079, Eukaryota |
GeneTreei | ENSGT00940000155917 |
HOGENOMi | CLU_034712_1_0_1 |
InParanoidi | Q920P5 |
OrthoDBi | 1378291at2759 |
PhylomeDBi | Q920P5 |
TreeFami | TF313747 |
Enzyme and pathway databases
Reactomei | R-MMU-499943, Interconversion of nucleotide di- and triphosphates |
Miscellaneous databases
BioGRID-ORCSi | 229949, 3 hits in 17 CRISPR screens |
ChiTaRSi | Ak5, mouse |
PROi | PR:Q920P5 |
RNActi | Q920P5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039058, Expressed in dentate gyrus of hippocampal formation granule cell and 183 other tissues |
Genevisiblei | Q920P5, MM |
Family and domain databases
CDDi | cd01428, ADK, 2 hits |
HAMAPi | MF_00235, Adenylate_kinase_Adk, 2 hits |
InterProi | View protein in InterPro IPR000850, Adenylat/UMP-CMP_kin IPR033690, Adenylat_kinase_CS IPR006267, AK1/5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR23359, PTHR23359, 3 hits |
PRINTSi | PR00094, ADENYLTKNASE |
SUPFAMi | SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR01360, aden_kin_iso1, 1 hit |
PROSITEi | View protein in PROSITE PS00113, ADENYLATE_KINASE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAD5_MOUSE | |
Accessioni | Q920P5Primary (citable) accession number: Q920P5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2002 |
Last sequence update: | September 1, 2009 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families