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UniProtKB - Q920N2 (BPL1_MOUSE)
Protein
Biotin--protein ligase
Gene
Hlcs
Organism
Mus musculus (Mouse)
Status
Functioni
Biotin--protein ligase catalyzing the biotinylation of the 4 biotin-dependent carboxylases acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase, and methylcrotonyl-CoA carboxylase.
By similarityCatalytic activityi
- apo-[methylmalonyl-CoA:pyruvate carboxytransferase] + ATP + biotin = AMP + diphosphate + H+ + holo-[methylmalonyl-CoA:pyruvate carboxytransferase]By similarityEC:6.3.4.9By similarityThis reaction proceeds in the forwardBy similarity direction.
- apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] + ATP + biotin = AMP + diphosphate + H+ + holo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]By similarityEC:6.3.4.10By similarityThis reaction proceeds in the forwardBy similarity direction.
- apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] + ATP + biotin = AMP + diphosphate + H+ + holo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)]By similarityEC:6.3.4.11By similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H+ + N6-biotinyl-L-lysyl-[protein]By similarityEC:6.3.4.15By similarityThis reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- biotin-[acetyl-CoA-carboxylase] ligase activity Source: GO_Central
- biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity Source: UniProtKB-EC
- biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity Source: UniProtKB-EC
- biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity Source: BHF-UCL
- biotin binding Source: BHF-UCL
- biotin-protein ligase activity Source: BHF-UCL
- enzyme binding Source: BHF-UCL
- identical protein binding Source: BHF-UCL
GO - Biological processi
- histone biotinylation Source: BHF-UCL
- histone modification Source: BHF-UCL
- protein biotinylation Source: BHF-UCL
- response to biotin Source: BHF-UCL
Keywordsi
Molecular function | Ligase, Multifunctional enzyme |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-196780, Biotin transport and metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Biotin--protein ligase (EC:6.3.4.-By similarity)Alternative name(s): Biotin apo-protein ligase Including the following 4 domains: Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9By similarity) Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10By similarity) Alternative name(s): Holocarboxylase synthetase Short name: HCS Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11By similarity) Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15By similarity) |
Gene namesi | Name:HlcsImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:894646, Hlcs |
VEuPathDBi | HostDB:ENSMUSG00000040820 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: BHF-UCL
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nuclear lamina Source: BHF-UCL
- nuclear matrix Source: BHF-UCL
Other locations
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064980 | 1 – 722 | Biotin--protein ligaseAdd BLAST | 722 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 295 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q920N2 |
PaxDbi | Q920N2 |
PRIDEi | Q920N2 |
ProteomicsDBi | 265457 |
PTM databases
iPTMneti | Q920N2 |
PhosphoSitePlusi | Q920N2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000040820, Expressed in lens of camera-type eye and 232 other tissues |
ExpressionAtlasi | Q920N2, baseline and differential |
Genevisiblei | Q920N2, MM |
Interactioni
Subunit structurei
Monomer.
By similarityGO - Molecular functioni
- enzyme binding Source: BHF-UCL
- identical protein binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 226044, 1 interactor |
STRINGi | 10090.ENSMUSP00000130981 |
Miscellaneous databases
RNActi | Q920N2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 459 – 648 | BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST | 190 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 93 | DisorderedSequence analysisAdd BLAST | 67 |
Sequence similaritiesi
Belongs to the biotin--protein ligase family.Curated
Phylogenomic databases
eggNOGi | KOG1536, Eukaryota |
GeneTreei | ENSGT00390000002960 |
HOGENOMi | CLU_006150_2_0_1 |
InParanoidi | Q920N2 |
OrthoDBi | 1392751at2759 |
PhylomeDBi | Q920N2 |
TreeFami | TF105860 |
Family and domain databases
CDDi | cd16442, BPL, 1 hit |
Gene3Di | 3.30.930.10, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR019197, Biotin-prot_ligase_N IPR004408, Biotin_CoA_COase_ligase IPR003142, BPL_C IPR004143, BPL_LPL_catalytic |
Pfami | View protein in Pfam PF02237, BPL_C, 1 hit PF03099, BPL_LplA_LipB, 1 hit PF09825, BPL_N, 1 hit |
SUPFAMi | SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00121, birA_ligase, 1 hit |
PROSITEi | View protein in PROSITE PS51733, BPL_LPL_CATALYTIC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q920N2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDRLQMDNG LIAQKIVSVH LKDPALKELG KASDKQVQGP PPGPEASPEA
60 70 80 90 100
QPAQGVMEHA GQGDCKAAGE GPSPRRRGCA PESEPAADGD PGLSSPELCQ
110 120 130 140 150
LHLSICHECL ELENSTIDSV RSASAENIPD LPCDHSGVEG AAGELCPERK
160 170 180 190 200
GKRVNISGKA PNILLYVGSG SEEALGRLQQ VRSVLTDCVD TDSYTLYHLL
210 220 230 240 250
EDSALRDPWS DNCLLLVIAS RDPIPKDIQH KFMAYLSQGG KVLGLSSPFT
260 270 280 290 300
LGGFRVTRRD VLRNTVQNLV FSKADGTEVR LSVLSSGYVY EEGPSLGRLQ
310 320 330 340 350
GHLENEDKDK MIVHVPFGTL GGEAVLCQVH LELPPGASLV QTADDFNVLK
360 370 380 390 400
SSNVRRHEVL KEILTALGLS CDAPQVPALT PLYLLLAAEE TQDPFMQWLG
410 420 430 440 450
RHTDPEGIIK SSKLSLQFVS SYTSEAEITP SSMPVVTDPE AFSSEHFSLE
460 470 480 490 500
TYRQNLQTTR LGKVILFAEV TSTTMSLLDG LMFEMPQEMG LIAIAVRQTQ
510 520 530 540 550
GKGRGPNAWL SPVGCALSTL LVFIPLRSQL GQRIPFVQHL MSLAVVEAVR
560 570 580 590 600
SIPGYEDINL RVKWPNDIYY SDLMKIGGVL VNSTLMGETF YILIGCGFNV
610 620 630 640 650
TNSNPTICIN DLIEEHNKQH GAGLKPLRAD CLIARAVTVL EKLIDRFQDQ
660 670 680 690 700
GPDGVLPLYY KYWVHGGQQV RLGSTEGPQA SIVGLDDSGF LQVHQEDGGV
710 720
VTVHPDGNSF DMLRNLIVPK RQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BPG4 | A0A2I3BPG4_MOUSE | Biotin--protein ligase | Hlcs | 869 | Annotation score: | ||
Q3TG10 | Q3TG10_MOUSE | Biotin--protein ligase | Hlcs | 327 | Annotation score: | ||
A0A2I3BQG1 | A0A2I3BQG1_MOUSE | Biotin--protein ligase | Hlcs | 107 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB066227 mRNA Translation: BAB68213.1 BC050090 mRNA Translation: AAH50090.1 |
CCDSi | CCDS28346.1 |
RefSeqi | NP_631884.1, NM_139145.4 XP_006522918.1, XM_006522855.2 XP_006522919.1, XM_006522856.2 |
Genome annotation databases
Ensembli | ENSMUST00000099512; ENSMUSP00000097112; ENSMUSG00000040820 ENSMUST00000163193; ENSMUSP00000130981; ENSMUSG00000040820 |
GeneIDi | 110948 |
KEGGi | mmu:110948 |
UCSCi | uc008aag.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB066227 mRNA Translation: BAB68213.1 BC050090 mRNA Translation: AAH50090.1 |
CCDSi | CCDS28346.1 |
RefSeqi | NP_631884.1, NM_139145.4 XP_006522918.1, XM_006522855.2 XP_006522919.1, XM_006522856.2 |
3D structure databases
AlphaFoldDBi | Q920N2 |
SMRi | Q920N2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 226044, 1 interactor |
STRINGi | 10090.ENSMUSP00000130981 |
PTM databases
iPTMneti | Q920N2 |
PhosphoSitePlusi | Q920N2 |
Proteomic databases
MaxQBi | Q920N2 |
PaxDbi | Q920N2 |
PRIDEi | Q920N2 |
ProteomicsDBi | 265457 |
Protocols and materials databases
Antibodypediai | 8381, 158 antibodies from 23 providers |
DNASUi | 110948 |
Genome annotation databases
Ensembli | ENSMUST00000099512; ENSMUSP00000097112; ENSMUSG00000040820 ENSMUST00000163193; ENSMUSP00000130981; ENSMUSG00000040820 |
GeneIDi | 110948 |
KEGGi | mmu:110948 |
UCSCi | uc008aag.1, mouse |
Organism-specific databases
CTDi | 3141 |
MGIi | MGI:894646, Hlcs |
VEuPathDBi | HostDB:ENSMUSG00000040820 |
Phylogenomic databases
eggNOGi | KOG1536, Eukaryota |
GeneTreei | ENSGT00390000002960 |
HOGENOMi | CLU_006150_2_0_1 |
InParanoidi | Q920N2 |
OrthoDBi | 1392751at2759 |
PhylomeDBi | Q920N2 |
TreeFami | TF105860 |
Enzyme and pathway databases
Reactomei | R-MMU-196780, Biotin transport and metabolism |
Miscellaneous databases
BioGRID-ORCSi | 110948, 11 hits in 80 CRISPR screens |
PROi | PR:Q920N2 |
RNActi | Q920N2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040820, Expressed in lens of camera-type eye and 232 other tissues |
ExpressionAtlasi | Q920N2, baseline and differential |
Genevisiblei | Q920N2, MM |
Family and domain databases
CDDi | cd16442, BPL, 1 hit |
Gene3Di | 3.30.930.10, 1 hit |
InterProi | View protein in InterPro IPR045864, aa-tRNA-synth_II/BPL/LPL IPR019197, Biotin-prot_ligase_N IPR004408, Biotin_CoA_COase_ligase IPR003142, BPL_C IPR004143, BPL_LPL_catalytic |
Pfami | View protein in Pfam PF02237, BPL_C, 1 hit PF03099, BPL_LplA_LipB, 1 hit PF09825, BPL_N, 1 hit |
SUPFAMi | SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00121, birA_ligase, 1 hit |
PROSITEi | View protein in PROSITE PS51733, BPL_LPL_CATALYTIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BPL1_MOUSE | |
Accessioni | Q920N2Primary (citable) accession number: Q920N2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2002 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families