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UniProtKB - Q92050 (WNT5B_DANRE)
Protein
Protein Wnt-5b
Gene
wnt5b
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Required during embryogenesis for extension of the primary anterior-posterior axis. Regulates convergent extension movements and hypaxial myogenesis during gastrulation via activation of non-canonical Wnt signaling (PubMed:22406073).
By similarity1 PublicationGO - Molecular functioni
- cytokine activity Source: GO_Central
- frizzled binding Source: GO_Central
- signaling receptor binding Source: ZFIN
GO - Biological processi
- ameboidal-type cell migration Source: ZFIN
- angiogenesis Source: ZFIN
- canonical Wnt signaling pathway Source: GO_Central
- cartilage morphogenesis Source: ZFIN
- cell fate commitment Source: GO_Central
- cell migration involved in gastrulation Source: ZFIN
- chondrocyte differentiation Source: ZFIN
- convergent extension Source: ZFIN
- convergent extension involved in axis elongation Source: ZFIN
- convergent extension involved in gastrulation Source: ZFIN
- dorsal convergence Source: ZFIN
- embryonic body morphogenesis Source: ZFIN
- embryonic cranial skeleton morphogenesis Source: ZFIN
- embryonic viscerocranium morphogenesis Source: ZFIN
- endochondral ossification Source: ZFIN
- endocrine pancreas development Source: ZFIN
- fin regeneration Source: ZFIN
- lymphangiogenesis Source: ZFIN
- midbrain-hindbrain boundary morphogenesis Source: ZFIN
- muscle cell differentiation Source: UniProtKB
- neuromast development Source: ZFIN
- neuron differentiation Source: GO_Central
- ossification Source: ZFIN
- otolith development Source: ZFIN
- positive regulation of convergent extension involved in gastrulation Source: UniProtKB
- positive regulation of non-canonical Wnt signaling pathway Source: UniProtKB
- post-anal tail morphogenesis Source: ZFIN
- regulation of Wnt signaling pathway, calcium modulating pathway Source: ZFIN
- release of sequestered calcium ion into cytosol Source: ZFIN
- skeletal system development Source: ZFIN
- somite development Source: ZFIN
- thrombocyte differentiation Source: ZFIN
- unidimensional cell growth Source: ZFIN
- Wnt signaling pathway, calcium modulating pathway Source: ZFIN
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-DRE-3238698, WNT ligand biogenesis and trafficking R-DRE-4086400, PCP/CE pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Wnt-5b |
Gene namesi | Name:wnt5b Synonyms:wnt-5, wnt5, wnt5a ORF Names:si:ch73-211c7.1 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-980526-87, wnt5b |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Disruption phenotypei
Morpholino knockdown results in convergent extension movement defects during gastrulation and an absence of myod1 expression in the majority of hypaxial muscle precursor cells.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000041438 | 22 – 363 | Protein Wnt-5bAdd BLAST | 342 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 87 ↔ 98 | By similarity | ||
Glycosylationi | 97 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 137 ↔ 145 | By similarity | ||
Disulfide bondi | 147 ↔ 165 | By similarity | ||
Disulfide bondi | 221 ↔ 235 | By similarity | ||
Disulfide bondi | 223 ↔ 230 | By similarity | ||
Lipidationi | 227 | O-palmitoleoyl serine; by PORCNBy similarity | 1 | |
Disulfide bondi | 292 ↔ 323 | By similarity | ||
Glycosylationi | 295 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 308 ↔ 318 | By similarity | ||
Glycosylationi | 309 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 322 ↔ 362 | By similarity | ||
Disulfide bondi | 338 ↔ 353 | By similarity | ||
Disulfide bondi | 340 ↔ 350 | By similarity | ||
Disulfide bondi | 345 ↔ 346 | By similarity |
Post-translational modificationi
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, LipoproteinProteomic databases
PaxDbi | Q92050 |
Expressioni
Developmental stagei
Expressed both maternally and zygotically (Ref. 1). Expressed in the germ ring at the blastoderm margin during the shield stage of gastrulation, expression is higher dorsally than ventrally (PubMed:22406073). Expressed at the posterior dorsal midline at the four-somite stage and in the pectoral fin bud 48 hours post-fertilization (PubMed:22406073). Zygotic expression begins during somitogenesis and is confined to the mesenchyme of the developing tailbud, the posterior 4-5 somites and the ventrolateral mesenchyme of the head (Ref. 1). In later stages, expressed in the pharyngeal arch mesenchyme and pectoral finbuds (Ref. 1). Not detected in mature chondrocytes (Ref. 1).2 Publications
Gene expression databases
Bgeei | ENSDARG00000102464, Expressed in gastrula and 43 other tissues |
ExpressionAtlasi | Q92050, baseline |
Interactioni
GO - Molecular functioni
- cytokine activity Source: GO_Central
- frizzled binding Source: GO_Central
- signaling receptor binding Source: ZFIN
Protein-protein interaction databases
BioGRIDi | 78343, 6 interactors |
STRINGi | 7955.ENSDARP00000106303 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Wnt family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000157617 |
HOGENOMi | CLU_033039_0_1_1 |
InParanoidi | Q92050 |
OMAi | EHARMLM |
PhylomeDBi | Q92050 |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR026537, Wnt5b IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit PTHR12027:SF87, PTHR12027:SF87, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q92050-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVRMNQGHL LLAVTLIVCN SQLLVVANSW WSLAMNPIQR PEMYIIGAQP
60 70 80 90 100
LCSQLTGLSQ GQRKLCQLYQ DHMVYIGEGA KTGIKECQYQ FRQRRWNCST
110 120 130 140 150
VDNTSVFGRV MHIGSRETAF TYAVSAAGVV NAVSRACREG ELSTCGCSRA
160 170 180 190 200
ARPRDLPRDW LWGGCGDNVN YGYRFAREFV DAREREKNYP RGSVEHARTL
210 220 230 240 250
MNLQNNEAGR MAVYNLANVA CKCHGVSGSC SLKTCWLQLA DFRRVGEFLK
260 270 280 290 300
EKYDSAAAMR INRRGKLELV NNRFNPPTGE DLVYIDPSPD YCLRNETTGS
310 320 330 340 350
LGTQGRLCNK TSEGMDGCEL MCCGRGYDQF KTYKHERCHC KFHWCCYVKC
360
KRCTSLVDQF VCK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51268 mRNA Translation: AAA96519.1 CT025936 Genomic DNA Translation: CAN88522.1 |
RefSeqi | NP_571012.1, NM_130937.1 XP_005164595.1, XM_005164538.3 XP_005164596.1, XM_005164539.3 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51268 mRNA Translation: AAA96519.1 CT025936 Genomic DNA Translation: CAN88522.1 |
RefSeqi | NP_571012.1, NM_130937.1 XP_005164595.1, XM_005164538.3 XP_005164596.1, XM_005164539.3 |
3D structure databases
AlphaFoldDBi | Q92050 |
SMRi | Q92050 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 78343, 6 interactors |
STRINGi | 7955.ENSDARP00000106303 |
Proteomic databases
PaxDbi | Q92050 |
Genome annotation databases
Organism-specific databases
CTDi | 81029 |
ZFINi | ZDB-GENE-980526-87, wnt5b |
Phylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000157617 |
HOGENOMi | CLU_033039_0_1_1 |
InParanoidi | Q92050 |
OMAi | EHARMLM |
PhylomeDBi | Q92050 |
Enzyme and pathway databases
Reactomei | R-DRE-3238698, WNT ligand biogenesis and trafficking R-DRE-4086400, PCP/CE pathway |
Miscellaneous databases
PROi | PR:Q92050 |
Gene expression databases
Bgeei | ENSDARG00000102464, Expressed in gastrula and 43 other tissues |
ExpressionAtlasi | Q92050, baseline |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR026537, Wnt5b IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit PTHR12027:SF87, PTHR12027:SF87, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WNT5B_DANRE | |
Accessioni | Q92050Primary (citable) accession number: Q92050 Secondary accession number(s): A5WVR7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families