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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5

Gene

Smarca5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 211ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Helicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (EC:3.6.4.-)
Alternative name(s):
Sucrose nonfermenting protein 2 homolog
Short name:
mSnf2h
Gene namesi
Name:Smarca5
Synonyms:Snf2h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1935129 Smarca5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000743552 – 1051SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5Add BLAST1050

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei55PhosphothreonineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei112PhosphothreonineCombined sources1
Modified residuei115PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei439N6-acetyllysineCombined sources1
Cross-linki643Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki646Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki693Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki721Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki734Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei754PhosphoserineBy similarity1
Cross-linki965Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91ZW3
MaxQBiQ91ZW3
PaxDbiQ91ZW3
PeptideAtlasiQ91ZW3
PRIDEiQ91ZW3

PTM databases

iPTMnetiQ91ZW3
PhosphoSitePlusiQ91ZW3

Expressioni

Tissue specificityi

Ubiquitously expressed.

Developmental stagei

Expressed in CD34-positive erythrocyte progenitor cells. Down-regulated upon differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000031715 Expressed in 312 organ(s), highest expression level in cumulus cell
GenevisibleiQ91ZW3 MM

Interactioni

Subunit structurei

Catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. ACF/WCRF contains BAZ1A; CHRAC contains BAZ1A, CHRAC1, and POLE3; RSF contains HBXAP; WICH contains BAZ1B/WSTF. SMARCA5 is the catalytic subunit of the NoRC chromatin-remodeling complex, which also contains BAZ2A/TIP5. The BAZ2A/TIP5 subunit of NoRC also interacts with DNMT1, DNMT3B and HDAC1, which allows NoRC to actively suppress rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation. Catalytic subunit of SMARCA5/cohesin/NuRD complexes. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Interacts with MYO1C. Interacts with BEND3 (By similarity).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220300, 17 interactors
ComplexPortaliCPX-1133 B-WICH chromatin remodelling complex
CPX-424 NoRC complex
CPX-444 ACF complex
CPX-463 RSF complex
CPX-841 WICH chromatin remodelling complex
CPX-858 CHRAC chromatin remodeling complex
CORUMiQ91ZW3
DIPiDIP-36073N
IntActiQ91ZW3, 18 interactors
MINTiQ91ZW3
STRINGi10090.ENSMUSP00000044361

Structurei

3D structure databases

ProteinModelPortaliQ91ZW3
SMRiQ91ZW3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 356Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini486 – 637Helicase C-terminalPROSITE-ProRule annotationAdd BLAST152
Domaini839 – 891SANT 1PROSITE-ProRule annotationAdd BLAST53
Domaini942 – 1006SANT 2PROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi307 – 310DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 13Poly-Pro7

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0385 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00900000140963
HOGENOMiHOG000192862
HOVERGENiHBG056329
InParanoidiQ91ZW3
KOiK11654
OMAiMAFTEWI
OrthoDBiEOG091G01GJ
PhylomeDBiQ91ZW3
TreeFamiTF300674

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00167 SANT, 2 hits
Gene3Di1.10.1040.30, 1 hit
3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR020838 DBINO
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR009057 Homeobox-like_sf
IPR029915 ISWI
IPR015194 ISWI_HAND-dom
IPR036306 ISWI_HAND-dom_sf
IPR027417 P-loop_NTPase
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR015195 SLIDE
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF887 PTHR10799:SF887, 1 hit
PfamiView protein in Pfam
PF13892 DBINO, 1 hit
PF09110 HAND, 1 hit
PF00271 Helicase_C, 1 hit
PF09111 SLIDE, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00717 SANT, 2 hits
SUPFAMiSSF101224 SSF101224, 1 hit
SSF46689 SSF46689, 2 hits
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51293 SANT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91ZW3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSAVEPPPP PPPESAPSKP SAAGAGGSSS GNKGGPEGGA APAAPCAAGS
60 70 80 90 100
GPADTEMEEV FDHGSPGKQK EIQEPDPTYE EKMQTDRANR FEYLLKQTEL
110 120 130 140 150
FAHFIQPAAQ KTPTSPLKMK PGRPRVKKDE KQNLLSVGDY RHRRTEQEED
160 170 180 190 200
EELLTESSKA TNVCTRFEDS PSYVKWGKLR DYQVRGLNWL ISLYENGING
210 220 230 240 250
ILADEMGLGK TLQTISLLGY MKHYRNIPGP HMVLVPKSTL HNWMSEFKKW
260 270 280 290 300
VPTLRSVCLI GDKEQRAAFV RDVLLPGEWD VCVTSYEMLI KEKSVFKKFN
310 320 330 340 350
WRYLVIDEAH RIKNEKSKLS EIVREFKTTN RLLLTGTPLQ NNLHELWSLL
360 370 380 390 400
NFLLPDVFNS ADDFDSWFDT NNCLGDQKLV ERLHMVLRPF LLRRIKADVE
410 420 430 440 450
KSLPPKKEVK IYVGLSKMQR EWYTRILMKD IDILNSAGKM DKMRLLNILM
460 470 480 490 500
QLRKCCNHPY LFDGAEPGPP YTTDMHLVTN SGKMVVLDKL LPKLKEQGSR
510 520 530 540 550
VLIFSQMTRV LDILEDYCMW RNYEYCRLDG QTPHDERQDS INAYNEPNST
560 570 580 590 600
KFVFMLSTRA GGLGINLATA DVVILYDSDW NPQVDLQAMD RAHRIGQTKT
610 620 630 640 650
VRVFRFITDN TVEERIVERA EMKLRLDSIV IQQGRLVDQN LNKIGKDEML
660 670 680 690 700
QMIRHGATHV FASKESEITD EDIDGILERG AKKTAEMNEK LSKMGESSLR
710 720 730 740 750
NFTMDTESSV YNFEGEDYRE KQKIAFTEWI EPPKRERKAN YAVDAYFREA
760 770 780 790 800
LRVSEPKAPK APRPPKQPNV QDFQFFPPRL FELLEKEILY YRKTIGYKVP
810 820 830 840 850
RSPDLPNAAQ AQKEEQLKID EAEPLNDEEL EEKEKLLTQG FTNWNKRDFN
860 870 880 890 900
QFIKANEKWG RDDIENIARE VEGKTPEEVI EYSAVFWERC NELQDIEKIM
910 920 930 940 950
AQIERGEARI QRRISIKKAL DTKIGRYKAP FHQLRISYGT NKGKNYTEEE
960 970 980 990 1000
DRFLICMLHK LGFDKENVYD ELRQCIRNSP QFRFDWFLKS RTAMELQRRC
1010 1020 1030 1040 1050
NTLITLIERE NMELEEKEKA EKKKRGPKPS TQKRKMDGAP DGRGRKKKLK

L
Length:1,051
Mass (Da):121,627
Last modified:December 1, 2001 - v1
Checksum:iC6CB69E7FADE73FC
GO

Sequence cautioni

The sequence AAH21922 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44 – 45AP → GS in AAK52454 (PubMed:11359880).Curated2
Sequence conflicti143R → C in AAK52454 (PubMed:11359880).Curated1
Sequence conflicti157S → R in AAK52454 (PubMed:11359880).Curated1
Sequence conflicti249K → R in AAK52454 (PubMed:11359880).Curated1
Sequence conflicti318K → N in AAK52454 (PubMed:11359880).Curated1
Sequence conflicti373C → S in AAK52454 (PubMed:11359880).Curated1
Sequence conflicti498G → S in AAK52454 (PubMed:11359880).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375046 mRNA Translation: AAL25793.1
AF325921 mRNA Translation: AAK52454.1
BC021922 mRNA Translation: AAH21922.1 Different initiation.
BC053069 mRNA Translation: AAH53069.1
AK039811 mRNA Translation: BAC30458.1
AK052320 mRNA Translation: BAC34934.2
CCDSiCCDS22442.1
RefSeqiNP_444354.2, NM_053124.2
UniGeneiMm.246803
Mm.474173

Genome annotation databases

EnsembliENSMUST00000043359; ENSMUSP00000044361; ENSMUSG00000031715
GeneIDi93762
KEGGimmu:93762
UCSCiuc009mja.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375046 mRNA Translation: AAL25793.1
AF325921 mRNA Translation: AAK52454.1
BC021922 mRNA Translation: AAH21922.1 Different initiation.
BC053069 mRNA Translation: AAH53069.1
AK039811 mRNA Translation: BAC30458.1
AK052320 mRNA Translation: BAC34934.2
CCDSiCCDS22442.1
RefSeqiNP_444354.2, NM_053124.2
UniGeneiMm.246803
Mm.474173

3D structure databases

ProteinModelPortaliQ91ZW3
SMRiQ91ZW3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220300, 17 interactors
ComplexPortaliCPX-1133 B-WICH chromatin remodelling complex
CPX-424 NoRC complex
CPX-444 ACF complex
CPX-463 RSF complex
CPX-841 WICH chromatin remodelling complex
CPX-858 CHRAC chromatin remodeling complex
CORUMiQ91ZW3
DIPiDIP-36073N
IntActiQ91ZW3, 18 interactors
MINTiQ91ZW3
STRINGi10090.ENSMUSP00000044361

PTM databases

iPTMnetiQ91ZW3
PhosphoSitePlusiQ91ZW3

Proteomic databases

EPDiQ91ZW3
MaxQBiQ91ZW3
PaxDbiQ91ZW3
PeptideAtlasiQ91ZW3
PRIDEiQ91ZW3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043359; ENSMUSP00000044361; ENSMUSG00000031715
GeneIDi93762
KEGGimmu:93762
UCSCiuc009mja.2 mouse

Organism-specific databases

CTDi8467
MGIiMGI:1935129 Smarca5

Phylogenomic databases

eggNOGiKOG0385 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00900000140963
HOGENOMiHOG000192862
HOVERGENiHBG056329
InParanoidiQ91ZW3
KOiK11654
OMAiMAFTEWI
OrthoDBiEOG091G01GJ
PhylomeDBiQ91ZW3
TreeFamiTF300674

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere

Miscellaneous databases

PROiPR:Q91ZW3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031715 Expressed in 312 organ(s), highest expression level in cumulus cell
GenevisibleiQ91ZW3 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00167 SANT, 2 hits
Gene3Di1.10.1040.30, 1 hit
3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR020838 DBINO
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR009057 Homeobox-like_sf
IPR029915 ISWI
IPR015194 ISWI_HAND-dom
IPR036306 ISWI_HAND-dom_sf
IPR027417 P-loop_NTPase
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR015195 SLIDE
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF887 PTHR10799:SF887, 1 hit
PfamiView protein in Pfam
PF13892 DBINO, 1 hit
PF09110 HAND, 1 hit
PF00271 Helicase_C, 1 hit
PF09111 SLIDE, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00717 SANT, 2 hits
SUPFAMiSSF101224 SSF101224, 1 hit
SSF46689 SSF46689, 2 hits
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51293 SANT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMCA5_MOUSE
AccessioniPrimary (citable) accession number: Q91ZW3
Secondary accession number(s): Q8C791
, Q8CA22, Q8VDG1, Q925M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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