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Protein

FYVE, RhoGEF and PH domain-containing protein 4

Gene

Fgd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates MAPK8 (By similarity). Plays a role in regulating the actin cytoskeleton and cell shape. Promotes the formation of lamellipodia.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri559 – 619FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Guanine-nucleotide releasing factor
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
FYVE, RhoGEF and PH domain-containing protein 4
Alternative name(s):
Actin filament-binding protein frabin
FGD1-related F-actin-binding protein
Gene namesi
Name:Fgd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2183747 Fgd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809481 – 766FYVE, RhoGEF and PH domain-containing protein 4Add BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei702PhosphoserineBy similarity1
Modified residuei716PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91ZT5
PeptideAtlasiQ91ZT5
PRIDEiQ91ZT5

PTM databases

iPTMnetiQ91ZT5
PhosphoSitePlusiQ91ZT5

Expressioni

Tissue specificityi

Detected in thymus, lung, heart, skeletal muscle, small intestine, liver, kidney, spleen and testis. Expressed in all parts of the brain and in the spinal cord at embryonic, postnatal, and adult stages. Levels of expression are lower in postnatal and adult tissues than in embryonic tissues.2 Publications

Gene expression databases

BgeeiENSMUSG00000022788 Expressed in 263 organ(s), highest expression level in brain
CleanExiMM_FGD4
ExpressionAtlasiQ91ZT5 baseline and differential
GenevisibleiQ91ZT5 MM

Interactioni

Subunit structurei

Homooligomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91ZT5, 2 interactors
STRINGi10090.ENSMUSP00000125174

Structurei

3D structure databases

ProteinModelPortaliQ91ZT5
SMRiQ91ZT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 393DHPROSITE-ProRule annotationAdd BLAST188
Domaini422 – 521PH 1PROSITE-ProRule annotationAdd BLAST100
Domaini643 – 740PH 2PROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 150Actin filament-bindingBy similarityAdd BLAST150

Domaini

The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri559 – 619FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4424 Eukaryota
ENOG410XRXV LUCA
GeneTreeiENSGT00920000148981
HOVERGENiHBG007506
InParanoidiQ91ZT5
KOiK05723
OrthoDBiEOG091G03FU
PhylomeDBiQ91ZT5
TreeFamiTF316247

Family and domain databases

CDDicd15791 PH1_FDG4, 1 hit
cd13236 PH2_FGD1-4, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 2 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR037742 FDG4_N_PH
IPR035941 FGD1-4_PH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF00169 PH, 2 hits
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00233 PH, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS50178 ZF_FYVE, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91ZT5-1) [UniParc]FASTAAdd to basket
Also known as: Frabin alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEESNPAPTS CTSKGKHSKV SDLISHFEGG SVLSSYIDLQ KDSTMNLNIP
60 70 80 90 100
QTLGQPGLTS SPPRKFLPQH SPQKQENDPD QTQGQHGCLA NGVVAAQNQM
110 120 130 140 150
ECEDEKETTL SPEMAIQTAA ASPDTHVLNG ERNETITDSA SSIANSHDEN
160 170 180 190 200
ASDSSCRTPG TDLGLPSKEG EPGMDAELQE RENGVNTMGL DTLDQHHEVK
210 220 230 240 250
ETNEQKLHKI ATELLLTERA YVSRLDLLDQ VFYCKLLEEA NRGSFPAEMV
260 270 280 290 300
NKIFSNISSI NAFHSKFLLP ELEKRMQEWE TTPRIGDILQ KLAPFLKMYG
310 320 330 340 350
EYVKGFDNAV ELVKTMTERV PQFKSVTEEI QKQKICGSLT LQHHMLEPIQ
360 370 380 390 400
RIPRYEMLLK DYLKKLSPDS PDWNDAKKSL EIISTAASHS NSAIRKMENL
410 420 430 440 450
KKLLEIYEML GEEEDIVNPS NELIKEGQIL KLAARNTSAQ ERYLFLFNNM
460 470 480 490 500
LLYCVPRFSL VGSKFTVRTR VGIDGMKIVE THNEEYPHTF QISGKERTLE
510 520 530 540 550
LQASSEQDKE EWIKALQESI DAFHQRHETF RNAIAKENDI PLEVSTAELG
560 570 580 590 600
KRAPRWIRDN EVTMCMKCKE SFNALTRRRH HCRACGHVVC WKCSDYKAQL
610 620 630 640 650
EYDGGRLNKV CKDCYQIISG FTDSEEKKRR GILEIESAEV SGNSEVCSFL
660 670 680 690 700
QYMEKSKPWQ KIWCVIPKQD PLVLYMYGAP QDVRAQATIP LLGYVVDDMP
710 720 730 740 750
KSADLPHSFK LTQSKSVHSF AADNEELKQK WLKIILLAVT GETPDGPSEH
760
LATLNNLPGP KKKSEC
Length:766
Mass (Da):86,541
Last modified:December 1, 2001 - v1
Checksum:i06BC1C01C6FCA274
GO
Isoform 2 (identifier: Q91ZT5-2) [UniParc]FASTAAdd to basket
Also known as: Frabin beta

The sequence of this isoform differs from the canonical sequence as follows:
     589-603: VCWKCSDYKAQLEYD → SEASSLSQLLEMVYR
     604-766: Missing.

Show »
Length:603
Mass (Da):68,210
Checksum:i645ED49AD97DC901
GO
Isoform 3 (identifier: Q91ZT5-3) [UniParc]FASTAAdd to basket
Also known as: Frabin gamma

The sequence of this isoform differs from the canonical sequence as follows:
     504-504: S → R
     505-766: Missing.

Show »
Length:504
Mass (Da):56,697
Checksum:i107EE4B4ECB43CE5
GO
Isoform 4 (identifier: Q91ZT5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     504-504: S → R
     505-766: Missing.

Note: No experimental confirmation available.
Show »
Length:460
Mass (Da):52,016
Checksum:iC1CF8B468DBA4718
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WGX0F8WGX0_MOUSE
FYVE, RhoGEF and PH domain-containi...
Fgd4
754Annotation score:
E0CZ69E0CZ69_MOUSE
FYVE, RhoGEF and PH domain-containi...
Fgd4
392Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36Y → C in BAC35703 (PubMed:16141072).Curated1
Sequence conflicti49I → V in BAC35703 (PubMed:16141072).Curated1
Sequence conflicti278E → K in BAE28995 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0130831 – 44Missing in isoform 4. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_013084504S → R in isoform 3 and isoform 4. 2 Publications1
Alternative sequenceiVSP_013085505 – 766Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST262
Alternative sequenceiVSP_013086589 – 603VCWKC…QLEYD → SEASSLSQLLEMVYR in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_013087604 – 766Missing in isoform 2. 1 PublicationAdd BLAST163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF402611 mRNA Translation: AAL05631.1
AF402612 mRNA Translation: AAL05632.1
AF402613 mRNA Translation: AAL05633.1
AK034504 mRNA Translation: BAC28733.1
AK054242 mRNA Translation: BAC35703.2
AK149626 mRNA Translation: BAE28995.1
BC129902 mRNA Translation: AAI29903.1
BC129903 mRNA Translation: AAI29904.1
CCDSiCCDS27986.1 [Q91ZT5-1]
RefSeqiNP_001288747.1, NM_001301818.1 [Q91ZT5-1]
NP_631978.1, NM_139232.3 [Q91ZT5-1]
XP_006522090.1, XM_006522027.3 [Q91ZT5-1]
XP_006522092.1, XM_006522029.2 [Q91ZT5-1]
UniGeneiMm.256131

Genome annotation databases

EnsembliENSMUST00000161188; ENSMUSP00000123763; ENSMUSG00000022788 [Q91ZT5-2]
ENSMUST00000161861; ENSMUSP00000125174; ENSMUSG00000022788 [Q91ZT5-1]
ENSMUST00000162671; ENSMUSP00000125736; ENSMUSG00000022788 [Q91ZT5-1]
GeneIDi224014
KEGGimmu:224014
UCSCiuc007yip.2 mouse [Q91ZT5-1]
uc007yis.1 mouse [Q91ZT5-2]
uc007yit.3 mouse [Q91ZT5-3]
uc007yiu.3 mouse [Q91ZT5-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF402611 mRNA Translation: AAL05631.1
AF402612 mRNA Translation: AAL05632.1
AF402613 mRNA Translation: AAL05633.1
AK034504 mRNA Translation: BAC28733.1
AK054242 mRNA Translation: BAC35703.2
AK149626 mRNA Translation: BAE28995.1
BC129902 mRNA Translation: AAI29903.1
BC129903 mRNA Translation: AAI29904.1
CCDSiCCDS27986.1 [Q91ZT5-1]
RefSeqiNP_001288747.1, NM_001301818.1 [Q91ZT5-1]
NP_631978.1, NM_139232.3 [Q91ZT5-1]
XP_006522090.1, XM_006522027.3 [Q91ZT5-1]
XP_006522092.1, XM_006522029.2 [Q91ZT5-1]
UniGeneiMm.256131

3D structure databases

ProteinModelPortaliQ91ZT5
SMRiQ91ZT5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91ZT5, 2 interactors
STRINGi10090.ENSMUSP00000125174

PTM databases

iPTMnetiQ91ZT5
PhosphoSitePlusiQ91ZT5

Proteomic databases

PaxDbiQ91ZT5
PeptideAtlasiQ91ZT5
PRIDEiQ91ZT5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000161188; ENSMUSP00000123763; ENSMUSG00000022788 [Q91ZT5-2]
ENSMUST00000161861; ENSMUSP00000125174; ENSMUSG00000022788 [Q91ZT5-1]
ENSMUST00000162671; ENSMUSP00000125736; ENSMUSG00000022788 [Q91ZT5-1]
GeneIDi224014
KEGGimmu:224014
UCSCiuc007yip.2 mouse [Q91ZT5-1]
uc007yis.1 mouse [Q91ZT5-2]
uc007yit.3 mouse [Q91ZT5-3]
uc007yiu.3 mouse [Q91ZT5-4]

Organism-specific databases

CTDi121512
MGIiMGI:2183747 Fgd4

Phylogenomic databases

eggNOGiKOG4424 Eukaryota
ENOG410XRXV LUCA
GeneTreeiENSGT00920000148981
HOVERGENiHBG007506
InParanoidiQ91ZT5
KOiK05723
OrthoDBiEOG091G03FU
PhylomeDBiQ91ZT5
TreeFamiTF316247

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Miscellaneous databases

PROiPR:Q91ZT5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022788 Expressed in 263 organ(s), highest expression level in brain
CleanExiMM_FGD4
ExpressionAtlasiQ91ZT5 baseline and differential
GenevisibleiQ91ZT5 MM

Family and domain databases

CDDicd15791 PH1_FDG4, 1 hit
cd13236 PH2_FGD1-4, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 2 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR037742 FDG4_N_PH
IPR035941 FGD1-4_PH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF00169 PH, 2 hits
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00233 PH, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS50178 ZF_FYVE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFGD4_MOUSE
AccessioniPrimary (citable) accession number: Q91ZT5
Secondary accession number(s): A1L355
, Q3UEB6, Q8BW60, Q8BZI7, Q91ZT3, Q91ZT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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