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Protein

Serine/threonine-protein kinase Nek8

Gene

Nek8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • determination of left/right symmetry Source: MGI
  • heart development Source: MGI
  • regulation of hippo signaling Source: MGI

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek8 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 8
Short name:
NimA-related protein kinase 8
Gene namesi
Name:Nek8
Synonyms:Jck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1890646 Nek8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

Defects in Nek8 are the cause of autosomal recessive juvenile polycystic kidney disease (ARJPKD).

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33K → M: Results in enlarged multinucleated cells. 1 Publication1
Mutagenesisi336L → F: Shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 Publication1
Mutagenesisi431H → Y: Shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 Publication1
Mutagenesisi503A → P: Shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number. 1 Publication1

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864331 – 698Serine/threonine-protein kinase Nek8Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91ZR4
PaxDbiQ91ZR4
PRIDEiQ91ZR4

PTM databases

PhosphoSitePlusiQ91ZR4

Expressioni

Tissue specificityi

Kidney, liver, and testis.

Gene expression databases

BgeeiENSMUSG00000017405 Expressed in 159 organ(s), highest expression level in membranous labyrinth
ExpressionAtlasiQ91ZR4 baseline and differential
GenevisibleiQ91ZR4 MM

Interactioni

Subunit structurei

Interacts with PKD2; may regulate PKD2 targeting to the cilium. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme (By similarity). Interacts with ANKS3 (PubMed:25671767).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
InvsO890192EBI-4282339,EBI-4281337

Protein-protein interaction databases

BioGridi228327, 2 interactors
CORUMiQ91ZR4
IntActiQ91ZR4, 1 interactor
MINTiQ91ZR4
STRINGi10090.ENSMUSP00000017549

Structurei

3D structure databases

ProteinModelPortaliQ91ZR4
SMRiQ91ZR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 258Protein kinasePROSITE-ProRule annotationAdd BLAST255
Repeati416 – 467RCC1 1Add BLAST52
Repeati468 – 519RCC1 2Add BLAST52
Repeati520 – 585RCC1 3Add BLAST66
Repeati586 – 637RCC1 4Add BLAST52
Repeati638 – 690RCC1 5Add BLAST53

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0589 Eukaryota
ENOG410Y7JF LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000006908
HOVERGENiHBG098799
InParanoidiQ91ZR4
KOiK20877
OMAiSAYAWGE
OrthoDBiEOG091G0GU4
PhylomeDBiQ91ZR4
TreeFamiTF106472

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00415 RCC1, 4 hits
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF50985 SSF50985, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50012 RCC1_3, 5 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q91ZR4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKYERIRVV GRGAFGIVHL CLRKADQKLV ILKQIPVEQM TKEERQAAQN
60 70 80 90 100
ECQVLKLLNH PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL
110 120 130 140 150
EEETILHFFV QILLALHHVH THLILHRDLK TQNILLDKHR MVVKIGDFGI
160 170 180 190 200
SKILSSKSKA YTVVGTPCYI SPELCEGKPY NQKSDIWALG CVLYELASLK
210 220 230 240 250
RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS LEPAQRPPLS
260 270 280 290 300
HIMAQPLCIR ALLNIHTDVG SVRMRRAEKS LTPGPPIASG STGSRATSAR
310 320 330 340 350
CRGVPRGPVR PAIPPPLSSV YAWGGGLSSP LRLPMLNTEV VQVAAGRTQK
360 370 380 390 400
AGVTRSGRLI LWEAPPLGAG GGTLLPGAVE LPQPQFVSRF LEGQSGVTIK
410 420 430 440 450
HVACGDLFTA CLTDRGIIMT FGSGSNGCLG HGNLTDISQP TIVEALLGYE
460 470 480 490 500
MVQVACGASH VLALSTDGEL FAWGRGDGGR LGLGTRESHN CPQQVPVAPG
510 520 530 540 550
QEAQRVVCGI DSSMILTSPG RVLACGSNRF NKLGLDHLSL DEEPVPYQQV
560 570 580 590 600
EEALSFTPLG SAPLDQEPLL CVDLGTAHSA AITASGDCYT FGSNQHGQLG
610 620 630 640 650
TSSRRVSRAP CRVQGLEGIK MVMVACGDAF TVAVGAEGEV YSWGKGTRGR
660 670 680 690
LGRRDEDAGL PRPVQLDETH PYMVTSVSCC HGNTLLAVRS VTDEPVPP
Length:698
Mass (Da):75,265
Last modified:December 1, 2001 - v1
Checksum:i686B29A8CF180E94
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q258E9Q258_MOUSE
Serine/threonine-protein kinase Nek...
Nek8
103Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326G → D in BAE32535 (PubMed:16141072).Curated1
Sequence conflicti528N → D in BAE32535 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti448G → V in ARJPKD. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407579 mRNA Translation: AAL09675.1
AK154358 mRNA Translation: BAE32535.1
BC070457 mRNA Translation: AAH70457.1
CCDSiCCDS25091.1
RefSeqiNP_543125.1, NM_080849.3
UniGeneiMm.23788

Genome annotation databases

EnsembliENSMUST00000017549; ENSMUSP00000017549; ENSMUSG00000017405
GeneIDi140859
KEGGimmu:140859
UCSCiuc007kih.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407579 mRNA Translation: AAL09675.1
AK154358 mRNA Translation: BAE32535.1
BC070457 mRNA Translation: AAH70457.1
CCDSiCCDS25091.1
RefSeqiNP_543125.1, NM_080849.3
UniGeneiMm.23788

3D structure databases

ProteinModelPortaliQ91ZR4
SMRiQ91ZR4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228327, 2 interactors
CORUMiQ91ZR4
IntActiQ91ZR4, 1 interactor
MINTiQ91ZR4
STRINGi10090.ENSMUSP00000017549

PTM databases

PhosphoSitePlusiQ91ZR4

Proteomic databases

MaxQBiQ91ZR4
PaxDbiQ91ZR4
PRIDEiQ91ZR4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017549; ENSMUSP00000017549; ENSMUSG00000017405
GeneIDi140859
KEGGimmu:140859
UCSCiuc007kih.2 mouse

Organism-specific databases

CTDi284086
MGIiMGI:1890646 Nek8

Phylogenomic databases

eggNOGiKOG0589 Eukaryota
ENOG410Y7JF LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000006908
HOVERGENiHBG098799
InParanoidiQ91ZR4
KOiK20877
OMAiSAYAWGE
OrthoDBiEOG091G0GU4
PhylomeDBiQ91ZR4
TreeFamiTF106472

Miscellaneous databases

PROiPR:Q91ZR4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017405 Expressed in 159 organ(s), highest expression level in membranous labyrinth
ExpressionAtlasiQ91ZR4 baseline and differential
GenevisibleiQ91ZR4 MM

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00415 RCC1, 4 hits
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF50985 SSF50985, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50012 RCC1_3, 5 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNEK8_MOUSE
AccessioniPrimary (citable) accession number: Q91ZR4
Secondary accession number(s): Q3U498, Q9D685
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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