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Protein

N-terminal EF-hand calcium-binding protein 2

Gene

Necab2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi76 – 871PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi110 – 1212PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 2
Short name:
EF-hand calcium-binding protein 2
Alternative name(s):
Neuronal calcium-binding protein 2
Gene namesi
Name:Necab2
Synonyms:Efcbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2152211 Necab2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002826141 – 389N-terminal EF-hand calcium-binding protein 2Add BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Omega-N-methylarginineCombined sources1
Modified residuei42Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ91ZP9
PRIDEiQ91ZP9

PTM databases

iPTMnetiQ91ZP9
PhosphoSitePlusiQ91ZP9

Expressioni

Tissue specificityi

Expressed in the iris, in the ciliary margin of the retina and in the inner portion of the neural retina. Expressed in the spinal dorsal horn with especially strong expression in lamina IIi; found in excitory synaptic boutons (at protein level).2 Publications

Developmental stagei

Expressed in retina, retinal pigmented epithelium, Rathke's pouch, corneal epithelium, the infundibulum and olfactory placodes at 10.5 dpc (at protein level). Expressed in the inner region of the neural retina, including the ganglion cell layer at 17.5 dpc (at protein level). Expressed in the optic sulcus and in the pre-tectum at 8.5 dpc. Expressed in the optic vesicle, in the midline position in the roof of the midbrain and in the pre-tectum at 9.0-9.5 dpc. Expressed in the olfactory placodes at 10.5 dpc. Expressed in retinal-pigmented epithelium and in the neural retina, with strong expression in the ciliary margin at 12.5-13.5 dpc.1 Publication

Inductioni

Up-regulated by PAX6.1 Publication

Gene expression databases

BgeeiENSMUSG00000031837 Expressed in 195 organ(s), highest expression level in habenula
CleanExiMM_NECAB2
GenevisibleiQ91ZP9 MM

Interactioni

Subunit structurei

Interacts (calcium-dependent) with ADORA2A and GRM5.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ91ZP9
SMRiQ91ZP9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 98EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini97 – 132EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini289 – 377ABMAdd BLAST89

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili173 – 198Sequence analysisAdd BLAST26

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IH3U Eukaryota
ENOG4111GJU LUCA
GeneTreeiENSGT00390000009734
HOGENOMiHOG000008120
HOVERGENiHBG050433
InParanoidiQ91ZP9
OMAiRDCFHVT
OrthoDBiEOG091G0DWL
PhylomeDBiQ91ZP9
TreeFamiTF331029

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR007138 ABM_dom
IPR011008 Dimeric_a/b-barrel
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR039862 NECAB1/2/3
PANTHERiPTHR12178 PTHR12178, 1 hit
PfamiView protein in Pfam
PF03992 ABM, 1 hit
PF13202 EF-hand_5, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS51725 ABM, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q91ZP9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCERAARLCR AGAHRLLREP PPQGRALGGL LRWVGARMGE PRAPLVPDIP
60 70 80 90 100
SADPGPGPAA SRGGTAVILD IFRRADKNDD GKLSLEEFQL FFADGVLNEK
110 120 130 140 150
ELEGLFHTID SDNTNHVDTK ELCDYFVEHM GDYEDVLASL ETLNHSVLKA
160 170 180 190 200
MGYTKKVYEG GSNVDQFVTR FLLKETANQI QSLLSSVESA VEAIEEQTSQ
210 220 230 240 250
IRQDHCKPSH AVNESRYGGP TPPYIPNHKL VAPEPMKSLP VATGEPKEDG
260 270 280 290 300
LEGQISRLAE LIGRLESKTL SFDLQQRLSD EEGTNMHLQL VRQEMAVCPE
310 320 330 340 350
QLSEFLDSLR QYLRSTAEER NCFHVAAVRM ADGLTFVIYE FWETEEEWKR
360 370 380
HLQSPVCKAF RHVKVDTLSQ PEALSQISVP AAWCTSGRD
Length:389
Mass (Da):43,441
Last modified:December 1, 2001 - v1
Checksum:i493F29BAFFD864EA
GO
Isoform 2 (identifier: Q91ZP9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: Missing.

Show »
Length:260
Mass (Da):29,297
Checksum:i86082FF5A60E300D
GO

Sequence cautioni

The sequence BAC38589 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0242151 – 129Missing in isoform 2. 1 PublicationAdd BLAST129

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411253 mRNA Translation: AAL04151.1
BC115857 mRNA Translation: AAI15858.1
BC115963 mRNA Translation: AAI15964.1
AK082727 mRNA Translation: BAC38589.1 Different initiation.
CCDSiCCDS22705.1 [Q91ZP9-1]
RefSeqiNP_473436.1, NM_054095.2 [Q91ZP9-1]
XP_017168015.1, XM_017312526.1 [Q91ZP9-2]
UniGeneiMm.356184

Genome annotation databases

EnsembliENSMUST00000098363; ENSMUSP00000095966; ENSMUSG00000031837 [Q91ZP9-1]
GeneIDi117148
KEGGimmu:117148
UCSCiuc009npq.1 mouse [Q91ZP9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411253 mRNA Translation: AAL04151.1
BC115857 mRNA Translation: AAI15858.1
BC115963 mRNA Translation: AAI15964.1
AK082727 mRNA Translation: BAC38589.1 Different initiation.
CCDSiCCDS22705.1 [Q91ZP9-1]
RefSeqiNP_473436.1, NM_054095.2 [Q91ZP9-1]
XP_017168015.1, XM_017312526.1 [Q91ZP9-2]
UniGeneiMm.356184

3D structure databases

ProteinModelPortaliQ91ZP9
SMRiQ91ZP9
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ91ZP9
PhosphoSitePlusiQ91ZP9

Proteomic databases

PaxDbiQ91ZP9
PRIDEiQ91ZP9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098363; ENSMUSP00000095966; ENSMUSG00000031837 [Q91ZP9-1]
GeneIDi117148
KEGGimmu:117148
UCSCiuc009npq.1 mouse [Q91ZP9-1]

Organism-specific databases

CTDi54550
MGIiMGI:2152211 Necab2

Phylogenomic databases

eggNOGiENOG410IH3U Eukaryota
ENOG4111GJU LUCA
GeneTreeiENSGT00390000009734
HOGENOMiHOG000008120
HOVERGENiHBG050433
InParanoidiQ91ZP9
OMAiRDCFHVT
OrthoDBiEOG091G0DWL
PhylomeDBiQ91ZP9
TreeFamiTF331029

Miscellaneous databases

PROiPR:Q91ZP9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031837 Expressed in 195 organ(s), highest expression level in habenula
CleanExiMM_NECAB2
GenevisibleiQ91ZP9 MM

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR007138 ABM_dom
IPR011008 Dimeric_a/b-barrel
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR039862 NECAB1/2/3
PANTHERiPTHR12178 PTHR12178, 1 hit
PfamiView protein in Pfam
PF03992 ABM, 1 hit
PF13202 EF-hand_5, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS51725 ABM, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNECA2_MOUSE
AccessioniPrimary (citable) accession number: Q91ZP9
Secondary accession number(s): Q1LZI4, Q8C492
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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