UniProtKB - Q91ZI8 (IOD3_MOUSE)
Thyroxine 5-deiodinase
Dio3
Functioni
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
By similarityCaution
Catalytic activityi
- EC:1.21.99.3By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 170 | 1 |
GO - Molecular functioni
- thyroxine 5'-deiodinase activity Source: MGI
- thyroxine 5-deiodinase activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: MGI
- brown fat cell proliferation Source: Ensembl
- hormone biosynthetic process Source: UniProtKB-KW
- positive regulation of multicellular organism growth Source: MGI
- response to hypoxia Source: Ensembl
- retinal cone cell apoptotic process Source: MGI
- retinal cone cell development Source: MGI
- thyroid hormone catabolic process Source: MGI
- thyroid hormone metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Thyroid hormones biosynthesis |
Enzyme and pathway databases
BRENDAi | 1.21.99.3, 3474 |
Reactomei | R-MMU-350864, Regulation of thyroid hormone activity |
Names & Taxonomyi
Protein namesi | Recommended name: Thyroxine 5-deiodinase (EC:1.21.99.3)Alternative name(s): 5DIII DIOIII Type 3 DI Type III iodothyronine deiodinase |
Gene namesi | Name:Dio3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1306782, Dio3 |
VEuPathDBi | HostDB:ENSMUSG00000075707 |
Subcellular locationi
Endosome
- Endosome membrane By similarity; Single-pass type II membrane protein By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Endosome
- endosome membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 44 | CytoplasmicSequence analysisAdd BLAST | 44 | |
Transmembranei | 45 – 67 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 68 – 304 | ExtracellularSequence analysisAdd BLAST | 237 |
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154324 | 1 – 304 | Thyroxine 5-deiodinaseAdd BLAST | 304 |
Proteomic databases
PaxDbi | Q91ZI8 |
PRIDEi | Q91ZI8 |
PTM databases
PhosphoSitePlusi | Q91ZI8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000075707, Expressed in placenta and 82 other tissues |
ExpressionAtlasi | Q91ZI8, baseline and differential |
Interactioni
Subunit structurei
Homodimer. May undergo minor heretodimerization with DIO1 and DIO2 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000133920 |
Miscellaneous databases
RNActi | Q91ZI8, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q91ZI8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 77 – 99 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S5FA, Eukaryota |
GeneTreei | ENSGT00940000154482 |
HOGENOMi | CLU_089345_0_0_1 |
InParanoidi | Q91ZI8 |
OMAi | GSCSXPP |
OrthoDBi | 977776at2759 |
PhylomeDBi | Q91ZI8 |
TreeFami | TF329721 |
Family and domain databases
InterProi | View protein in InterPro IPR000643, Iodothyronine_deiodinase IPR008261, Iodothyronine_deiodinase_AS IPR027252, Iodothyronine_deiodinase_I/III IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11781, PTHR11781, 1 hit |
Pfami | View protein in Pfam PF00837, T4_deiodinase, 1 hit |
PIRSFi | PIRSF001330, IOD, 1 hit PIRSF500144, IODI_III, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS01205, T4_DEIODINASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPRQAASRLV VGEGEGPPGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP
60 70 80 90 100
RFLGTAFMLW LLDFLCIRKH FLRRRHPDHP EPEVELNSEG EEMPPDDPPI
110 120 130 140 150
CVSDDNRLCT LASLKAVWHG QKLDFFKQAH EGGPAPNSEV VRPDGFQSQR
160 170 180 190 200
ILDYAQGTRP LVLNFGSCTU PPFMARMSAF QRLVTKYQRD VDFLIIYIEE
210 220 230 240 250
AHPSDGWVTT DSPYVIPQHR SLEDRVSAAR VLQQGAPGCA LVLDTMANSS
260 270 280 290 300
SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGTR
PHRF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketK3W4Q2 | K3W4Q2_MOUSE | Iodothyronine deiodinase | Dio3 | 131 | Annotation score: |
Sequence cautioni
Non-standard residue
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-standard residuei | 170 | Selenocysteine | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF426023 Genomic DNA Translation: AAL23960.1 Different initiation. AK140797 mRNA Translation: BAE24483.1 Different initiation. AK146060 mRNA Translation: BAE26869.1 Different initiation. AL591207 Genomic DNA No translation available. BC106847 mRNA Translation: AAI06848.1 Different initiation. BC106848 mRNA Translation: AAI06849.1 Different initiation. |
CCDSi | CCDS26171.2 |
RefSeqi | NP_742117.2, NM_172119.2 |
Genome annotation databases
Ensembli | ENSMUST00000173014; ENSMUSP00000133920; ENSMUSG00000075707 |
GeneIDi | 107585 |
KEGGi | mmu:107585 |
UCSCi | uc007pbi.1, mouse |
Keywords - Coding sequence diversityi
SelenocysteineSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF426023 Genomic DNA Translation: AAL23960.1 Different initiation. AK140797 mRNA Translation: BAE24483.1 Different initiation. AK146060 mRNA Translation: BAE26869.1 Different initiation. AL591207 Genomic DNA No translation available. BC106847 mRNA Translation: AAI06848.1 Different initiation. BC106848 mRNA Translation: AAI06849.1 Different initiation. |
CCDSi | CCDS26171.2 |
RefSeqi | NP_742117.2, NM_172119.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4TR3 | X-ray | 1.90 | A | 120-304 | [»] | |
4TR4 | X-ray | 1.93 | A | 120-304 | [»] | |
SMRi | Q91ZI8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000133920 |
PTM databases
PhosphoSitePlusi | Q91ZI8 |
Proteomic databases
PaxDbi | Q91ZI8 |
PRIDEi | Q91ZI8 |
Protocols and materials databases
Antibodypediai | 56330, 314 antibodies from 26 providers |
DNASUi | 107585 |
Genome annotation databases
Ensembli | ENSMUST00000173014; ENSMUSP00000133920; ENSMUSG00000075707 |
GeneIDi | 107585 |
KEGGi | mmu:107585 |
UCSCi | uc007pbi.1, mouse |
Organism-specific databases
CTDi | 1735 |
MGIi | MGI:1306782, Dio3 |
VEuPathDBi | HostDB:ENSMUSG00000075707 |
Phylogenomic databases
eggNOGi | ENOG502S5FA, Eukaryota |
GeneTreei | ENSGT00940000154482 |
HOGENOMi | CLU_089345_0_0_1 |
InParanoidi | Q91ZI8 |
OMAi | GSCSXPP |
OrthoDBi | 977776at2759 |
PhylomeDBi | Q91ZI8 |
TreeFami | TF329721 |
Enzyme and pathway databases
BRENDAi | 1.21.99.3, 3474 |
Reactomei | R-MMU-350864, Regulation of thyroid hormone activity |
Miscellaneous databases
BioGRID-ORCSi | 107585, 1 hit in 65 CRISPR screens |
PROi | PR:Q91ZI8 |
RNActi | Q91ZI8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000075707, Expressed in placenta and 82 other tissues |
ExpressionAtlasi | Q91ZI8, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR000643, Iodothyronine_deiodinase IPR008261, Iodothyronine_deiodinase_AS IPR027252, Iodothyronine_deiodinase_I/III IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11781, PTHR11781, 1 hit |
Pfami | View protein in Pfam PF00837, T4_deiodinase, 1 hit |
PIRSFi | PIRSF001330, IOD, 1 hit PIRSF500144, IODI_III, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS01205, T4_DEIODINASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IOD3_MOUSE | |
Accessioni | Q91ZI8Primary (citable) accession number: Q91ZI8 Secondary accession number(s): G3UY28, Q3UKD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2002 |
Last sequence update: | May 14, 2014 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families