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UniProtKB - Q91ZF0 (DJC24_MOUSE)
Protein
DnaJ homolog subfamily C member 24
Gene
Dnajc24
Organism
Mus musculus (Mouse)
Status
Functioni
The iron-bound form is redox-active and can function as electron carrier (By similarity).
Stimulates the ATPase activity of several Hsp70-type chaperones. This ability is enhanced by iron-binding. Plays a role in the diphthamide biosynthesis, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).
By similarity2 Publications: peptidyl-diphthamide biosynthesis Pathwayi
This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 114 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 116 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 135 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 138 | ZincPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATPase activator activity Source: UniProtKB
- ferrous iron binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: UniProtKB-UniPathway
- positive regulation of ATP-dependent activity Source: UniProtKB
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5358493, Synthesis of diphthamide-EEF2 |
UniPathwayi | UPA00559 |
Names & Taxonomyi
Protein namesi | Recommended name: DnaJ homolog subfamily C member 24Alternative name(s): CSL-type zinc finger-containing protein 3 Diphthamide biosynthesis protein 4 J domain protein DjC7 |
Gene namesi | Name:Dnajc24 Synonyms:Dph4, Zcsl3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919522, Dnajc24 |
VEuPathDBi | HostDB:ENSMUSG00000027166 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
High embryonic lethality at 14.5 dpc. As early as 10.5 dpc, embryos are smaller than their wild-type littermates. Embryos that survive long enough to initiate digit formation show one or more additional preaxial digits.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082623 | 1 – 148 | DnaJ homolog subfamily C member 24Add BLAST | 148 |
Proteomic databases
EPDi | Q91ZF0 |
MaxQBi | Q91ZF0 |
PaxDbi | Q91ZF0 |
PRIDEi | Q91ZF0 |
ProteomicsDBi | 279714 |
PTM databases
PhosphoSitePlusi | Q91ZF0 |
Expressioni
Tissue specificityi
Detected in heart, brain, spleen, lung, liver, kidney and testis.1 Publication
Gene expression databases
ExpressionAtlasi | Q91ZF0, baseline and differential |
Interactioni
Subunit structurei
Monomer and homooligomer. Iron binding promotes oligomerization (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099615 |
Miscellaneous databases
RNActi | Q91ZF0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q91ZF0 |
BMRBi | Q91ZF0 |
SMRi | Q91ZF0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q91ZF0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 81 | JPROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 92 – 147 | DPH-type MBPROSITE-ProRule annotationAdd BLAST | 56 |
Domaini
The DPH-type metal-binding (MB) domain can bind either zinc or iron ions.PROSITE-ProRule annotation
Sequence similaritiesi
Belongs to the DPH4 family.Curated
Phylogenomic databases
eggNOGi | KOG0715, Eukaryota |
GeneTreei | ENSGT00390000005430 |
InParanoidi | Q91ZF0 |
OMAi | SWPVDAC |
OrthoDBi | 1580122at2759 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit 3.10.660.10, 1 hit |
InterProi | View protein in InterPro IPR007872, DHP_MB_dom IPR036671, DHP_MB_sf IPR001623, DnaJ_domain IPR042978, DNAJC24 IPR036869, J_dom_sf |
PANTHERi | PTHR45255, PTHR45255, 1 hit |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF05207, zf-CSL, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF144217, SSF144217, 1 hit SSF46565, SSF46565, 1 hit |
PROSITEi | View protein in PROSITE PS50076, DNAJ_2, 1 hit PS51074, DPH_MB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q91ZF0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALEQTLKKD WYSILGADPS ANMSDLKQKY QKLILLYHPD KQSADVPAGT
60 70 80 90 100
MEECMQKFIE IDQAWKILGN EETKKKYDLQ RHEDELRNVG PVDAQVRLEE
110 120 130 140
MSWNQGDESF FLSCRCGGKY TVSKDEAQEA TLISCDACSL IVELLHQS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7CGC9 | F7CGC9_MOUSE | DnaJ homolog subfamily C member 24 | Dnajc24 | 116 | Annotation score: |
Sequence cautioni
The sequence AAK21968 differs from that shown. A nucleotide mismatch versus the mouse genome results in the formation of an upstream ATG start codon.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | S → F in AAK21968 (PubMed:11595173).Curated | 1 | |
Sequence conflicti | 146 | H → Q in AAK21968 (PubMed:11595173).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028460 mRNA Translation: AAK21968.1 Sequence problems. AK006521 mRNA Translation: BAB24631.1 AL590380 Genomic DNA No translation available. AL928773 Genomic DNA No translation available. BX649381 Genomic DNA No translation available. BC030072 mRNA Translation: AAH30072.1 BC091774 mRNA Translation: AAH91774.1 |
CCDSi | CCDS16501.1 |
RefSeqi | NP_081268.1, NM_026992.3 |
Genome annotation databases
Ensembli | ENSMUST00000102555; ENSMUSP00000099615; ENSMUSG00000027166 |
GeneIDi | 99349 |
KEGGi | mmu:99349 |
UCSCi | uc008llf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY028460 mRNA Translation: AAK21968.1 Sequence problems. AK006521 mRNA Translation: BAB24631.1 AL590380 Genomic DNA No translation available. AL928773 Genomic DNA No translation available. BX649381 Genomic DNA No translation available. BC030072 mRNA Translation: AAH30072.1 BC091774 mRNA Translation: AAH91774.1 |
CCDSi | CCDS16501.1 |
RefSeqi | NP_081268.1, NM_026992.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WJZ | NMR | - | A | 1-81 | [»] | |
AlphaFoldDBi | Q91ZF0 | |||||
BMRBi | Q91ZF0 | |||||
SMRi | Q91ZF0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099615 |
PTM databases
PhosphoSitePlusi | Q91ZF0 |
Proteomic databases
EPDi | Q91ZF0 |
MaxQBi | Q91ZF0 |
PaxDbi | Q91ZF0 |
PRIDEi | Q91ZF0 |
ProteomicsDBi | 279714 |
Protocols and materials databases
Antibodypediai | 12795, 110 antibodies from 22 providers |
DNASUi | 99349 |
Genome annotation databases
Ensembli | ENSMUST00000102555; ENSMUSP00000099615; ENSMUSG00000027166 |
GeneIDi | 99349 |
KEGGi | mmu:99349 |
UCSCi | uc008llf.2, mouse |
Organism-specific databases
CTDi | 120526 |
MGIi | MGI:1919522, Dnajc24 |
VEuPathDBi | HostDB:ENSMUSG00000027166 |
Phylogenomic databases
eggNOGi | KOG0715, Eukaryota |
GeneTreei | ENSGT00390000005430 |
InParanoidi | Q91ZF0 |
OMAi | SWPVDAC |
OrthoDBi | 1580122at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00559 |
Reactomei | R-MMU-5358493, Synthesis of diphthamide-EEF2 |
Miscellaneous databases
BioGRID-ORCSi | 99349, 7 hits in 73 CRISPR screens |
ChiTaRSi | Dnajc24, mouse |
EvolutionaryTracei | Q91ZF0 |
PROi | PR:Q91ZF0 |
RNActi | Q91ZF0, protein |
SOURCEi | Search... |
Gene expression databases
ExpressionAtlasi | Q91ZF0, baseline and differential |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit |
Gene3Di | 1.10.287.110, 1 hit 3.10.660.10, 1 hit |
InterProi | View protein in InterPro IPR007872, DHP_MB_dom IPR036671, DHP_MB_sf IPR001623, DnaJ_domain IPR042978, DNAJC24 IPR036869, J_dom_sf |
PANTHERi | PTHR45255, PTHR45255, 1 hit |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF05207, zf-CSL, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF144217, SSF144217, 1 hit SSF46565, SSF46565, 1 hit |
PROSITEi | View protein in PROSITE PS50076, DNAJ_2, 1 hit PS51074, DPH_MB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DJC24_MOUSE | |
Accessioni | Q91ZF0Primary (citable) accession number: Q91ZF0 Secondary accession number(s): Q9D9S7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2005 |
Last sequence update: | September 12, 2018 | |
Last modified: | May 25, 2022 | |
This is version 145 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families