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Protein

ELL-associated factor 2

Gene

Eaf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities (By similarity). Acts as a transcriptional transactivator of TCEA1 elongation activity.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
ELL-associated factor 2
Alternative name(s):
Ehrlich S-II transcriptional activator factor
Testosterone-regulated apoptosis inducer and tumor suppressor protein
Gene namesi
Name:Eaf2
Synonyms:Festa, Traits
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2146616 Eaf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001303381 – 262ELL-associated factor 2Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei146PhosphoserineBy similarity1
Modified residuei151PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91ZD6
PRIDEiQ91ZD6

PTM databases

iPTMnetiQ91ZD6
PhosphoSitePlusiQ91ZD6

Expressioni

Tissue specificityi

Isoform 1 is expressed in ovary, uterus, mammary glands, brain, spleen, liver, lung, thymus, kidney, skeletal muscle, skin and testis. Isoform 2 is expressed in kidney.2 Publications

Developmental stagei

Expressed in brain and spinal cord at 10 dpc. Expressed in brain, spinal cord, cranial and spinal ganglia, lens, retina, cochlea, olfactory epithelium and pituitary at 12 dpc. Expressed in intestine, bladder endothelium, retinal ganglion cells, nephrons, bronchial epithelium, secretory epithelium of submandibular glands, tubular epithelium of the epididymis, ectodermal invaginations of mammary buds and vibrissae follicles, incisors and molars at 15 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000022838 Expressed in 132 organ(s), highest expression level in brain
CleanExiMM_EAF2
ExpressionAtlasiQ91ZD6 baseline and differential
GenevisibleiQ91ZD6 MM

Interactioni

Subunit structurei

Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL and ELL2 (By similarity). Isoform 1 and isoform 2 interact with TCEA1.By similarity1 Publication

Protein-protein interaction databases

BioGridi223046, 1 interactor
IntActiQ91ZD6, 1 interactor
STRINGi10090.ENSMUSP00000110477

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 104Necessary for interaction with ELLBy similarityAdd BLAST88
Regioni177 – 262Necessary for transactivation activityBy similarityAdd BLAST86
Regioni248 – 262Necessary for interaction with TCEA1 and transactivation activity1 PublicationAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi126 – 130Poly-Gln5
Compositional biasi174 – 219Ser-richAdd BLAST46

Sequence similaritiesi

Belongs to the EAF family.Curated

Phylogenomic databases

eggNOGiKOG4795 Eukaryota
ENOG4111KQJ LUCA
GeneTreeiENSGT00390000017724
HOGENOMiHOG000070137
HOVERGENiHBG054898
InParanoidiQ91ZD6
KOiK15186
OMAiECILIVN
OrthoDBiEOG091G0MMP
PhylomeDBiQ91ZD6
TreeFamiTF320864

Family and domain databases

InterProiView protein in InterPro
IPR027093 EAF_fam
IPR019194 Tscrpt_elong_fac_Eaf_N
PANTHERiPTHR15970 PTHR15970, 1 hit
PfamiView protein in Pfam
PF09816 EAF, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91ZD6-1) [UniParc]FASTAAdd to basket
Also known as: FESTA-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGPAGLAYL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSCEGN
60 70 80 90 100
LEVGKGEQVT ITLPNIEGST PPVTVFKGSK RPYLKECILI INHDTGECRL
110 120 130 140 150
EKLSSNITVK KTRVEGSSRI QYRLEQQQQQ MWNLPRTSNL VQHSPSEEKM
160 170 180 190 200
SPTSLMDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDD
210 220 230 240 250
DQFSPLGPRK YSSEHPSMSA GPQYRTSEAD ATCHRLQDHS TLLMSTLRSD
260
LQLSESESDS ED
Length:262
Mass (Da):29,187
Last modified:December 1, 2001 - v1
Checksum:i5D072D84816B766A
GO
Isoform 2 (identifier: Q91ZD6-2) [UniParc]FASTAAdd to basket
Also known as: FESTA-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Show »
Length:132
Mass (Da):14,534
Checksum:iEB80830042DBD1F2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K4DI60K4DI60_MOUSE
ELL associated factor 2, isoform CR...
Eaf2 mCG_128244
195Annotation score:

Sequence cautioni

The sequence AAH04721 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC77525 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153111 – 130Missing in isoform 2. 2 PublicationsAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049021 mRNA Translation: AAL12224.1
AY034479 mRNA Translation: AAK59701.1
AB081298 mRNA Translation: BAC77525.1 Different initiation.
BC004721 mRNA Translation: AAH04721.1 Different initiation.
BC056626 mRNA Translation: AAH56626.1
CCDSiCCDS37335.1 [Q91ZD6-2]
CCDS49843.1 [Q91ZD6-1]
RefSeqiNP_001106872.1, NM_001113401.1 [Q91ZD6-1]
NP_001106876.1, NM_001113405.1 [Q91ZD6-2]
NP_598872.1, NM_134111.2 [Q91ZD6-2]
XP_006521746.1, XM_006521683.1 [Q91ZD6-2]
XP_006521747.1, XM_006521684.3 [Q91ZD6-2]
XP_006521748.1, XM_006521685.3 [Q91ZD6-2]
XP_006521749.1, XM_006521686.2
UniGeneiMm.432078

Genome annotation databases

EnsembliENSMUST00000075946; ENSMUSP00000075331; ENSMUSG00000022838 [Q91ZD6-2]
ENSMUST00000114825; ENSMUSP00000110473; ENSMUSG00000022838 [Q91ZD6-2]
ENSMUST00000114829; ENSMUSP00000110477; ENSMUSG00000022838 [Q91ZD6-1]
GeneIDi106389
KEGGimmu:106389
UCSCiuc007zcx.1 mouse [Q91ZD6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049021 mRNA Translation: AAL12224.1
AY034479 mRNA Translation: AAK59701.1
AB081298 mRNA Translation: BAC77525.1 Different initiation.
BC004721 mRNA Translation: AAH04721.1 Different initiation.
BC056626 mRNA Translation: AAH56626.1
CCDSiCCDS37335.1 [Q91ZD6-2]
CCDS49843.1 [Q91ZD6-1]
RefSeqiNP_001106872.1, NM_001113401.1 [Q91ZD6-1]
NP_001106876.1, NM_001113405.1 [Q91ZD6-2]
NP_598872.1, NM_134111.2 [Q91ZD6-2]
XP_006521746.1, XM_006521683.1 [Q91ZD6-2]
XP_006521747.1, XM_006521684.3 [Q91ZD6-2]
XP_006521748.1, XM_006521685.3 [Q91ZD6-2]
XP_006521749.1, XM_006521686.2
UniGeneiMm.432078

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223046, 1 interactor
IntActiQ91ZD6, 1 interactor
STRINGi10090.ENSMUSP00000110477

PTM databases

iPTMnetiQ91ZD6
PhosphoSitePlusiQ91ZD6

Proteomic databases

PaxDbiQ91ZD6
PRIDEiQ91ZD6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075946; ENSMUSP00000075331; ENSMUSG00000022838 [Q91ZD6-2]
ENSMUST00000114825; ENSMUSP00000110473; ENSMUSG00000022838 [Q91ZD6-2]
ENSMUST00000114829; ENSMUSP00000110477; ENSMUSG00000022838 [Q91ZD6-1]
GeneIDi106389
KEGGimmu:106389
UCSCiuc007zcx.1 mouse [Q91ZD6-1]

Organism-specific databases

CTDi55840
MGIiMGI:2146616 Eaf2

Phylogenomic databases

eggNOGiKOG4795 Eukaryota
ENOG4111KQJ LUCA
GeneTreeiENSGT00390000017724
HOGENOMiHOG000070137
HOVERGENiHBG054898
InParanoidiQ91ZD6
KOiK15186
OMAiECILIVN
OrthoDBiEOG091G0MMP
PhylomeDBiQ91ZD6
TreeFamiTF320864

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiEaf2 mouse
PROiPR:Q91ZD6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022838 Expressed in 132 organ(s), highest expression level in brain
CleanExiMM_EAF2
ExpressionAtlasiQ91ZD6 baseline and differential
GenevisibleiQ91ZD6 MM

Family and domain databases

InterProiView protein in InterPro
IPR027093 EAF_fam
IPR019194 Tscrpt_elong_fac_Eaf_N
PANTHERiPTHR15970 PTHR15970, 1 hit
PfamiView protein in Pfam
PF09816 EAF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEAF2_MOUSE
AccessioniPrimary (citable) accession number: Q91ZD6
Secondary accession number(s): Q7TN80, Q99KD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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