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Protein

Notch-regulated ankyrin repeat-containing protein

Gene

Nrarp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Downstream effector of Notch signaling. Involved in the regulation of liver cancer cells self-renewal (By similarity). Involved in the regulation of canonical Wnt signaling by stabilizing LEF1 (By similarity). Involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. Proposed to integrate endothelial Notch and Wnt signaling to control stalk cell proliferation and to stablilize new endothelial connections during angiogenesis (PubMed:19154719). During somitogenesis involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae. Required for proper anterior-posterior somite patterning. Proposed to function in a negative feedback loop to destabilize Notch 1 intracellular domain (NICD) and downregulate the Notch signal, preventing expansion of the Notch signal into the anterior somite domain (PubMed:21795391, PubMed:21998026).By similarity1 Publication4 Publications

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Notch-regulated ankyrin repeat-containing protein
Gene namesi
Name:Nrarp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914372 Nrarp

Pathology & Biotechi

Disruption phenotypei

Axial skeletal defects in newborn mice such as rib fusions, fused vertebral bodies and fused pedicles of the vertebrae; defects are influenced by the strain-specific genetic background. Embryos exhibit expansion and fusion of myotomes, dorsal root ganglia fusions and defects in projection of the spinal nerves. Embryos show increased level of Notch 1 intracellular domain (NICD) expression in presomitic mesoderm and somites (PubMed:21998026). In contrast, fewer somites and vertebrae found in -/- are linked to a longer segmentation clock period (PubMed:21795391). Defects in the radial expansion of the vascular plexus from the optic nerve head to the periphery, reduction of retinal vessel density; however, most of the defects in angiogenesis resolve over time (PubMed:19154719).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003250801 – 114Notch-regulated ankyrin repeat-containing proteinAdd BLAST114

Proteomic databases

PaxDbiQ91ZA8
PRIDEiQ91ZA8

Expressioni

Tissue specificityi

Expressed at high levels in the brain, heart, colon, kidney, liver, lung and small intestine. Expressed in retina, most prominent in endothelial cells at the migrating point of the vasculature behind leading tip cells. Expressed in testis.3 Publications

Developmental stagei

During embryogenesis, expressed in several tissues in which cellular differentiation is regulated by the notch signaling pathway. At E10.5 expressed in intersomatic vessels and in vessels of the limb buds with strongest expression at vascular branch points. Cyclic expression between 8.5 dpc and 10.5 dpc somitogenesis is dependent on DLL3.3 Publications

Inductioni

By Notch signaling via a CBF1/Su(H)/Lag-1 (CSL)-dependent pathway.1 Publication

Gene expression databases

BgeeiENSMUSG00000078202 Expressed in 273 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_NRARP
GenevisibleiQ91ZA8 MM

Interactioni

Subunit structurei

Interacts with LEF1.1 Publication

Protein-protein interaction databases

IntActiQ91ZA8, 1 interactor
STRINGi10090.ENSMUSP00000100615

Structurei

3D structure databases

ProteinModelPortaliQ91ZA8
SMRiQ91ZA8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 79ANK 1Add BLAST30
Repeati83 – 112ANK 2Add BLAST30

Sequence similaritiesi

Belongs to the NRARP family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0505 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00760000119141
HOGENOMiHOG000082661
HOVERGENiHBG097373
InParanoidiQ91ZA8
OMAiHQDIALY
OrthoDBiEOG091G0WZR
PhylomeDBiQ91ZA8

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q91ZA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQAELSTCS APQTQRIFQE AVRKGNTQEL QSLLQNMTNC EFNVNSFGPE
60 70 80 90 100
GQTALHQSVI DGNLELVKLL VKFGADIRLA NRDGWSALHI AAFGGHQDIV
110
LYLITKAKYA ASGR
Length:114
Mass (Da):12,492
Last modified:December 1, 2001 - v1
Checksum:iC8B84B648DB47F4F
GO
Isoform 2 (identifier: Q91ZA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-80: LA → RS
     81-114: Missing.

Show »
Length:80
Mass (Da):8,868
Checksum:i737E8265B85E9A60
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03238079 – 80LA → RS in isoform 2. 1 Publication2
Alternative sequenceiVSP_03238181 – 114Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY046077 mRNA Translation: AAL05944.1
AK012426 mRNA Translation: BAB28231.1 Sequence problems.
AL732309 Genomic DNA No translation available.
BC048088 mRNA Translation: AAH48088.1
BC069891 mRNA Translation: AAH69891.1
CCDSiCCDS38066.1 [Q91ZA8-1]
RefSeqiNP_080256.2, NM_025980.2 [Q91ZA8-1]
UniGeneiMm.46539

Genome annotation databases

EnsembliENSMUST00000104999; ENSMUSP00000100615; ENSMUSG00000078202 [Q91ZA8-1]
GeneIDi67122
KEGGimmu:67122
UCSCiuc008iqk.1 mouse [Q91ZA8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY046077 mRNA Translation: AAL05944.1
AK012426 mRNA Translation: BAB28231.1 Sequence problems.
AL732309 Genomic DNA No translation available.
BC048088 mRNA Translation: AAH48088.1
BC069891 mRNA Translation: AAH69891.1
CCDSiCCDS38066.1 [Q91ZA8-1]
RefSeqiNP_080256.2, NM_025980.2 [Q91ZA8-1]
UniGeneiMm.46539

3D structure databases

ProteinModelPortaliQ91ZA8
SMRiQ91ZA8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91ZA8, 1 interactor
STRINGi10090.ENSMUSP00000100615

Proteomic databases

PaxDbiQ91ZA8
PRIDEiQ91ZA8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000104999; ENSMUSP00000100615; ENSMUSG00000078202 [Q91ZA8-1]
GeneIDi67122
KEGGimmu:67122
UCSCiuc008iqk.1 mouse [Q91ZA8-1]

Organism-specific databases

CTDi441478
MGIiMGI:1914372 Nrarp

Phylogenomic databases

eggNOGiKOG0505 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00760000119141
HOGENOMiHOG000082661
HOVERGENiHBG097373
InParanoidiQ91ZA8
OMAiHQDIALY
OrthoDBiEOG091G0WZR
PhylomeDBiQ91ZA8

Miscellaneous databases

PROiPR:Q91ZA8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078202 Expressed in 273 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_NRARP
GenevisibleiQ91ZA8 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNRARP_MOUSE
AccessioniPrimary (citable) accession number: Q91ZA8
Secondary accession number(s): Q80UV7, Q9CZM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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