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Protein

SLIT-ROBO Rho GTPase-activating protein 2

Gene

Srgap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. May play a role for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processNeurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle
R-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
SLIT-ROBO Rho GTPase-activating protein 2
Short name:
srGAP2
Alternative name(s):
Formin-binding protein 2
Formin-binding protein 27
Short name:
FBP-27
Gene namesi
Name:Srgap2
Synonyms:Fbp27, Fnbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:109605 Srgap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice are viable and show no abnormality of cortical lamination. However, a delay in dendritic spine maturation coupled to an increase in spine neck and spine density is observed.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi527R → L: Unable to stimulate RAC1 GTPase activity and to induce neurite branching. No effect on filopodia biogenesis and neurite outgrowth. 1 Publication1
Mutagenesisi765W → A: Loss of the ability to induce filopodia and to initiate neurite outgrowht. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567681 – 1071SLIT-ROBO Rho GTPase-activating protein 2Add BLAST1071

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei206PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei500PhosphoserineCombined sources1
Modified residuei691PhosphoserineBy similarity1
Modified residuei695PhosphoserineCombined sources1
Modified residuei724PhosphoserineBy similarity1
Modified residuei795PhosphoserineBy similarity1
Modified residuei916PhosphoserineBy similarity1
Modified residuei927Omega-N-methylated arginine; by PRMT5By similarity1
Modified residuei930PhosphoserineBy similarity1
Modified residuei990PhosphoserineCombined sources1
Modified residuei994PhosphoserineBy similarity1
Modified residuei1013PhosphoserineBy similarity1
Modified residuei1027PhosphoserineBy similarity1

Post-translational modificationi

Methylation at Arg-927 is required for the stimulation of cell migration, dimerization and localization at the plasma membrane protrusions.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ91Z67
MaxQBiQ91Z67
PaxDbiQ91Z67
PeptideAtlasiQ91Z67
PRIDEiQ91Z67

PTM databases

iPTMnetiQ91Z67
PhosphoSitePlusiQ91Z67

Expressioni

Developmental stagei

Expressed throughout cortical development culminating at P1. Expression is reduced but still present in the adult cortex. Expressed in the cortical wall both in neuronal progenitors in the ventricular zone and post-mitotic neurons in the cortical plate (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026425 Expressed in 281 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SRGAP2
ExpressionAtlasiQ91Z67 baseline and differential
GenevisibleiQ91Z67 MM

Interactioni

Subunit structurei

Homodimer. Interacts with FASLG (By similarity). Interacts with PRMT5 (By similarity). Probably interacts with ROBO1 and ROBO2. Interacts with RAC1; specifically stimulates RAC1 GTPase activity. Interacts (via SH3 domain) with FMNL1 (activated by RAC1); regulates the actin filament severing activity of FMNL1. Interacts (via SH3 domain) with FMNL3. Interacts (via SH3 domain) with GPHN.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199721, 5 interactors
IntActiQ91Z67, 2 interactors
STRINGi10090.ENSMUSP00000095195

Structurei

3D structure databases

ProteinModelPortaliQ91Z67
SMRiQ91Z67
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 325F-BARPROSITE-ProRule annotationAdd BLAST304
Domaini489 – 679Rho-GAPPROSITE-ProRule annotationAdd BLAST191
Domaini728 – 787SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili363 – 401Sequence analysisAdd BLAST39
Coiled coili940 – 968Sequence analysisAdd BLAST29

Domaini

The F-BAR domain mediates oligomerization, binds membranes, and induces plasma membrane protrusions.

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3565 Eukaryota
ENOG4110QCJ LUCA
GeneTreeiENSGT00760000118863
HOGENOMiHOG000039980
HOVERGENiHBG051637
InParanoidiQ91Z67
KOiK07526
OrthoDBiEOG091G0113
PhylomeDBiQ91Z67
TreeFamiTF315892

Family and domain databases

CDDicd11955 SH3_srGAP1-3, 1 hit
Gene3Di1.10.555.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035648 srGAP1/2/3_SH3
IPR030252 srGAP2
PANTHERiPTHR14166:SF6 PTHR14166:SF6, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00620 RhoGAP, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00324 RhoGAP, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50238 RHOGAP, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q91Z67-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSPAKFKKD KEIIAEYDTQ VKEIRAQLTE QMKCLDQQCE LRVQLLQDLQ
60 70 80 90 100
DFFRKKAEIE MDYSRNLEKL AERFLAKTRS TKDQQFKKDQ NVLSPVNCWN
110 120 130 140 150
LLLNQVKRES RDHTTLSDIY LNNIIPRFVQ VSEDSGRLFK KSKEVGQQLQ
160 170 180 190 200
DDLMKVLNEL YSVMKTYHMY NADSISAQSK LKEAEKQEEK QIGKSVKQED
210 220 230 240 250
RQTPRSPDST ANVRIEEKHV RRSSVKKIEK MKEKRQAKYT ENKLKAIKAR
260 270 280 290 300
NEYLLALEAT NASVFKYYIH DLSDIIDQCC DLGYHASLNR ALRTFLSAEL
310 320 330 340 350
NLEQSKHEGL DAIENAVENL DATSDKQRLM EMYNNVFCPP MKFEFQPHMG
360 370 380 390 400
DMASQLCAQQ PVQSELVQRC QQLQSRLSTL KIENEEVKKT MEATLQTIQD
410 420 430 440 450
IVTVEDFDVS DCFQYSNSME SVKSTVSETF MSKPSIAKRR ANQQETEQFY
460 470 480 490 500
FTKMKEYLEG RNLITKLQAK HDLLQKTLGE SQRTDCSLAR RSSTVRKQDS
510 520 530 540 550
SQAIPLVVES CIRFISRHGL QHEGIFRVSG SQVEVNDIKN AFERGEDPLA
560 570 580 590 600
GDQNDHDMDS IAGVLKLYFR GLEHPLFPKD IFHDLIACVT MDNLQERAVH
610 620 630 640 650
IRKVLLVLPK PTLIIMRYLF AFLNHLSQFS EENMMDPYNL AICFGPSLMS
660 670 680 690 700
VPEGHDQVSC QAHVNELIKT IIIQHENIFP NPRELEGPIY SRGGSMEDYC
710 720 730 740 750
DSTHGETTSA EDSTQDVTAE HHTSDDECEP IEAIAKFDYV GRTARELSFK
760 770 780 790 800
KGASLLLYQR ASDDWWEGRH NGIDGLIPHQ YIVVQDTEDG VVERSSPKSE
810 820 830 840 850
IEVMSEPPEE KVTARTGASC PSGGHVADIY LANINKQRKR PESGSIRKAF
860 870 880 890 900
RSDSHGLGSS LTDSSSLGVG ASCRPSSQPI MSQNLPKEGP DKCSISGHGS
910 920 930 940 950
LNSISRHSSL KNRMDSPQIR KTATAGRSKS FNNHRPMDPE VIAQDIEATM
960 970 980 990 1000
NSALNELQEL ERQSSAKHTP DVVLDTLEPL KTSPVVAPTS EPSSPLHTQL
1010 1020 1030 1040 1050
LKDPEPAFQR SASTAGDIAC AFRPVKSVKM AAPVKPPATR PKPTVFPKTN
1060 1070
ATSPGVNSSA SPQATDKSCT V
Length:1,071
Mass (Da):120,798
Last modified:January 9, 2007 - v2
Checksum:i9093C63476BA605D
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WNM1A0A087WNM1_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
858Annotation score:
A0A087WSQ1A0A087WSQ1_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
649Annotation score:
A0A087WS59A0A087WS59_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
451Annotation score:
A0A087WRV4A0A087WRV4_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
110Annotation score:
A0A087WNR5A0A087WNR5_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
55Annotation score:
A0A087WS73A0A087WS73_MOUSE
SLIT-ROBO Rho GTPase-activating pro...
Srgap2
267Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti598A → V in AAL27032 (PubMed:11672528).Curated1
Sequence conflicti612T → S in AAL27032 (PubMed:11672528).Curated1
Sequence conflicti662A → S in AAL27032 (PubMed:11672528).Curated1
Sequence conflicti737F → C in AAL27032 (PubMed:11672528).Curated1
Sequence conflicti765W → L in AAL27032 (PubMed:11672528).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109299 Genomic DNA No translation available.
AC120217 Genomic DNA No translation available.
AC165436 Genomic DNA No translation available.
BC151081 mRNA Translation: AAI51082.1
BC151082 mRNA Translation: AAI51083.1
BC158055 mRNA Translation: AAI58056.1
BC172152 mRNA Translation: AAI72152.1
AY057900 mRNA Translation: AAL27032.1
AK132220 mRNA Translation: BAE21041.1
U40752 mRNA Translation: AAC52480.1
CCDSiCCDS48355.1
PIRiS64712
RefSeqiNP_001074480.2, NM_001081011.2
XP_006529197.1, XM_006529134.3
UniGeneiMm.276259

Genome annotation databases

EnsembliENSMUST00000097588; ENSMUSP00000095195; ENSMUSG00000026425
GeneIDi14270
KEGGimmu:14270
UCSCiuc007cng.2 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

Branching out - Issue 143 of October 2012

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109299 Genomic DNA No translation available.
AC120217 Genomic DNA No translation available.
AC165436 Genomic DNA No translation available.
BC151081 mRNA Translation: AAI51082.1
BC151082 mRNA Translation: AAI51083.1
BC158055 mRNA Translation: AAI58056.1
BC172152 mRNA Translation: AAI72152.1
AY057900 mRNA Translation: AAL27032.1
AK132220 mRNA Translation: BAE21041.1
U40752 mRNA Translation: AAC52480.1
CCDSiCCDS48355.1
PIRiS64712
RefSeqiNP_001074480.2, NM_001081011.2
XP_006529197.1, XM_006529134.3
UniGeneiMm.276259

3D structure databases

ProteinModelPortaliQ91Z67
SMRiQ91Z67
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199721, 5 interactors
IntActiQ91Z67, 2 interactors
STRINGi10090.ENSMUSP00000095195

PTM databases

iPTMnetiQ91Z67
PhosphoSitePlusiQ91Z67

Proteomic databases

EPDiQ91Z67
MaxQBiQ91Z67
PaxDbiQ91Z67
PeptideAtlasiQ91Z67
PRIDEiQ91Z67

Protocols and materials databases

DNASUi14270
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097588; ENSMUSP00000095195; ENSMUSG00000026425
GeneIDi14270
KEGGimmu:14270
UCSCiuc007cng.2 mouse

Organism-specific databases

CTDi23380
MGIiMGI:109605 Srgap2

Phylogenomic databases

eggNOGiKOG3565 Eukaryota
ENOG4110QCJ LUCA
GeneTreeiENSGT00760000118863
HOGENOMiHOG000039980
HOVERGENiHBG051637
InParanoidiQ91Z67
KOiK07526
OrthoDBiEOG091G0113
PhylomeDBiQ91Z67
TreeFamiTF315892

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle
R-MMU-5663220 RHO GTPases Activate Formins

Miscellaneous databases

ChiTaRSiSrgap2 mouse
PROiPR:Q91Z67
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026425 Expressed in 281 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SRGAP2
ExpressionAtlasiQ91Z67 baseline and differential
GenevisibleiQ91Z67 MM

Family and domain databases

CDDicd11955 SH3_srGAP1-3, 1 hit
Gene3Di1.10.555.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035648 srGAP1/2/3_SH3
IPR030252 srGAP2
PANTHERiPTHR14166:SF6 PTHR14166:SF6, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00620 RhoGAP, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00324 RhoGAP, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50238 RHOGAP, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRGP2_MOUSE
AccessioniPrimary (citable) accession number: Q91Z67
Secondary accession number(s): B2RY13, Q3V1V8, Q61054
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 9, 2007
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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