UniProtKB - Q91YX0 (THMS2_MOUSE)
Protein
Protein THEMIS2
Gene
Themis2
Organism
Mus musculus (Mouse)
Status
Functioni
May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production.1 Publication
GO - Biological processi
- inflammatory response Source: UniProtKB-KW
- T cell receptor signaling pathway Source: MGI
Keywordsi
Biological process | Immunity, Inflammatory response |
Names & Taxonomyi
Protein namesi | Recommended name: Protein THEMIS2Alternative name(s): Induced by contact to basement membrane 1 protein Short name: Protein ICB-1 Thymocyte-expressed molecule involved in selection protein 2 |
Gene namesi | Name:Themis2 Synonyms:Icb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2446213, Themis2 |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 552 | P → A: Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-555. Partial loss of enhancement of LPS-induced TNF production; when associated with A-555. | 1 | |
Mutagenesisi | 555 | P → A: Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-552. Partial loss of enhancement of LPS-induced TNF production; when associated with A-552. | 1 | |
Mutagenesisi | 660 | Y → F: Loss of phosphorylation. Loss of LYN-binding. Slight decrease in GRB2-binding. No effect on VAV-binding. Partial loss of enhancement of LPS-induced TNF production. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084144 | 1 – 663 | Protein THEMIS2Add BLAST | 663 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 596 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 660 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Phosphorylation at Tyr-660 is induced by LPS.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q91YX0 |
jPOSTi | Q91YX0 |
MaxQBi | Q91YX0 |
PaxDbi | Q91YX0 |
PeptideAtlasi | Q91YX0 |
PRIDEi | Q91YX0 |
PTM databases
iPTMneti | Q91YX0 |
PhosphoSitePlusi | Q91YX0 |
Expressioni
Tissue specificityi
Restricted to B-cells, macrophages and dendritic cells. Down-regulated in splenocytes of mice developing arthritis in a collagen-induced model, not in those of mice failing to develop the disease. Transiently down-regulated in splenocytes of mice infected with influenza virus.1 Publication
Developmental stagei
Up-regulated during the differentiation of bone marrow precursors into macrophages.1 Publication
Inductioni
Up-regulated by pro-inflammatory stimuli, such as IFNG (at protein level). Down-regulated by anti-inflammatory stimuli, such as TGFB1 and dexamethasone (at protein level).
Gene expression databases
Bgeei | ENSMUSG00000037731, Expressed in granulocyte and 160 other tissues |
Genevisiblei | Q91YX0, MM |
Interactioni
Subunit structurei
When phosphorylated, interacts with LYN.
Interacts with VAV1 and GRB2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 231027, 1 interactor |
STRINGi | 10090.ENSMUSP00000036945 |
Miscellaneous databases
RNActi | Q91YX0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 237 | CABIT 1Add BLAST | 236 | |
Regioni | 238 – 515 | CABIT 2Add BLAST | 278 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 403 – 419 | Glu-richAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the themis family.Curated
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
HOGENOMi | CLU_022319_0_0_1 |
InParanoidi | Q91YX0 |
OMAi | EIPPQWL |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q91YX0 |
TreeFami | TF333479 |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
i Sequence
Sequence statusi: Complete.
Q91YX0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPVPLQDFV SGLDPTSLPR VLRVCSGVYF EGSVYELFGN ECCLSTGDLI
60 70 80 90 100
KVTHVQLQKV VCEYPETGQT LELNPNFTGL FSPLTSLRSY RTLEDLVSAM
110 120 130 140 150
PQNSTRWPIY FKSTQRIVTK ASVVPEDQPL RLEAVEIHHG IRYARCVQVS
160 170 180 190 200
KTKELLHLPL SQKGPFWRCK PSAPQTLHQI LQDPALKDLT LSCPSLPWNS
210 220 230 240 250
VILKPQYMLQ AIMHMRSSIV KIPSTLEVEV EDVTASSQHI HFFKPLRLSE
260 270 280 290 300
VLAGGGPFPL TTEILEVPEG PPVFLSPWVS FLRKGQRLCI YGPASPSWRV
310 320 330 340 350
VASSKSRKVP RYFMLSGAYQ GKLKRRPREF STAYDLLGAL QPGRPLRVVA
360 370 380 390 400
TKDCDGNEEE NPDFSFLAVG DRLEVLRSGQ VCGTKGQDID VLVCQRLSEQ
410 420 430 440 450
SGEEEEDLEE IEDEAEDKEQ ILLPLYLSGS FVEEVNDSRR YNLVDLTAQY
460 470 480 490 500
SLPCEVKVVT KDTRHPTDPL ASFPGLRLEE KLTEPFLVVS LDSQPEMCFE
510 520 530 540 550
IPPRWLDLTV VEAEGQPAQV ARPLSIAPVE ELSEAFYYSL RKLPASESQA
560 570 580 590 600
PPPRPPKSQG INKKQQNIQS CKESSVKPQV VEPQKSCPQP QLKAKTLEAL
610 620 630 640 650
PKNKSNVYSK ISVHKKDRKP NPQTQNSVLS MKPKTSSSLG KHSTMESHLL
660
PDPDMDDHDY EEI
Sequence cautioni
The sequence AAH13712 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK150471 mRNA Translation: BAE29588.1 AK153536 mRNA Translation: BAE32073.1 AK155536 mRNA Translation: BAE33313.1 AK171009 mRNA Translation: BAE42180.1 AL627130 Genomic DNA No translation available. AL671190 Genomic DNA No translation available. BC013712 mRNA Translation: AAH13712.1 Different initiation. BC151122 mRNA Translation: AAI51123.1 BC151126 mRNA Translation: AAI51127.1 |
CCDSi | CCDS18735.1 |
RefSeqi | NP_001028480.1, NM_001033308.2 |
Genome annotation databases
Ensembli | ENSMUST00000045154; ENSMUSP00000036945; ENSMUSG00000037731 |
GeneIDi | 230787 |
KEGGi | mmu:230787 |
UCSCi | uc008vbv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK150471 mRNA Translation: BAE29588.1 AK153536 mRNA Translation: BAE32073.1 AK155536 mRNA Translation: BAE33313.1 AK171009 mRNA Translation: BAE42180.1 AL627130 Genomic DNA No translation available. AL671190 Genomic DNA No translation available. BC013712 mRNA Translation: AAH13712.1 Different initiation. BC151122 mRNA Translation: AAI51123.1 BC151126 mRNA Translation: AAI51127.1 |
CCDSi | CCDS18735.1 |
RefSeqi | NP_001028480.1, NM_001033308.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 231027, 1 interactor |
STRINGi | 10090.ENSMUSP00000036945 |
PTM databases
iPTMneti | Q91YX0 |
PhosphoSitePlusi | Q91YX0 |
Proteomic databases
EPDi | Q91YX0 |
jPOSTi | Q91YX0 |
MaxQBi | Q91YX0 |
PaxDbi | Q91YX0 |
PeptideAtlasi | Q91YX0 |
PRIDEi | Q91YX0 |
Protocols and materials databases
Antibodypediai | 30867, 98 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000045154; ENSMUSP00000036945; ENSMUSG00000037731 |
GeneIDi | 230787 |
KEGGi | mmu:230787 |
UCSCi | uc008vbv.1, mouse |
Organism-specific databases
CTDi | 9473 |
MGIi | MGI:2446213, Themis2 |
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
HOGENOMi | CLU_022319_0_0_1 |
InParanoidi | Q91YX0 |
OMAi | EIPPQWL |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q91YX0 |
TreeFami | TF333479 |
Miscellaneous databases
BioGRID-ORCSi | 230787, 1 hit in 18 CRISPR screens |
ChiTaRSi | Themis2, mouse |
PROi | PR:Q91YX0 |
RNActi | Q91YX0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037731, Expressed in granulocyte and 160 other tissues |
Genevisiblei | Q91YX0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THMS2_MOUSE | |
Accessioni | Q91YX0Primary (citable) accession number: Q91YX0 Secondary accession number(s): Q3U5K5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 100 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families