Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

YTH domain-containing family protein 2

Gene

Ythdf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability (PubMed:28867294). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing (PubMed:28867294). Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation (By similarity). Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (PubMed:28867294). Also involved in haematopoietic stem cells specification: acts by binding to m6A-containing mRNAs, leading to decrease Notch dignaling and promote endothelial to haematopoietic transition (By similarity). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei422N6-methyladenosineBy similarity1
Binding sitei462N6-methyladenosineBy similarity1
Binding sitei486N6-methyladenosineBy similarity1
Binding sitei491N6-methyladenosineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processDifferentiation, Oogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
YTH domain-containing family protein 2Curated
Gene namesi
Name:Ythdf2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444233 Ythdf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Female-specific infertility despite successful ovulation (PubMed:28867294). Corpora lutea is present in ovaries, indicating that ovulation has occurred (PubMed:28867294). Defects during oocyte maturation are probably caused by the deregulation of some transcripts, leading to an arrest prior to or at the two-cell stage, with various cytokinesis defects observed in the two-cell embryos.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004255442 – 579YTH domain-containing family protein 2Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei22PhosphoserineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei196PhosphoserineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei394PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ91YT7
MaxQBiQ91YT7
PaxDbiQ91YT7
PeptideAtlasiQ91YT7
PRIDEiQ91YT7

PTM databases

iPTMnetiQ91YT7
PhosphoSitePlusiQ91YT7

Expressioni

Tissue specificityi

Widely expressed, with highest expression in testis.1 Publication

Developmental stagei

Expressed in the germline during gametogenesis: expressed at all stages of spermatogenesis, with elevated expression observed in pachytene spermatocytes (PubMed:28867294). During oogenesis, expressed at all stages of folliculogenesis: expressed both in the oocyte and in somatic granulosa cells (PubMed:28867294). Also expressed during oocyte maturation, with abundant expression in germinal vesicle as well as in meiosis II oocytes (PubMed:28867294).1 Publication

Gene expression databases

BgeeiENSMUSG00000040025
ExpressionAtlasiQ91YT7 baseline and differential
GenevisibleiQ91YT7 MM

Interactioni

Subunit structurei

Interacts with YTHDF3.By similarity

Protein-protein interaction databases

BioGridi229450, 2 interactors
IntActiQ91YT7, 2 interactors
STRINGi10090.ENSMUSP00000120414

Structurei

3D structure databases

ProteinModelPortaliQ91YT7
SMRiQ91YT7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini410 – 544YTHPROSITE-ProRule annotationAdd BLAST135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 384Localization to mRNA processing bodies (P-bodies)By similarityAdd BLAST383
Regioni385 – 579Interaction with m6A-containing mRNAsBy similarityAdd BLAST195
Regioni416 – 418N6-methyladenosine bindingBy similarity3
Regioni432 – 433N6-methyladenosine bindingBy similarity2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi290 – 347Gln-richAdd BLAST58

Sequence similaritiesi

Belongs to the YTHDF2 family.Curated

Phylogenomic databases

eggNOGiKOG1901 Eukaryota
ENOG410YABQ LUCA
GeneTreeiENSGT00390000017549
HOVERGENiHBG060315
InParanoidiQ91YT7
KOiK20102
OMAiKDFDWNP
OrthoDBiEOG091G0D17
PhylomeDBiQ91YT7
TreeFamiTF323736

Family and domain databases

InterProiView protein in InterPro
IPR007275 YTH_domain
PfamiView protein in Pfam
PF04146 YTH, 1 hit
PROSITEiView protein in PROSITE
PS50882 YTH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASSLLEQR PKGQGNKVQN GSVHQKDGLN DDDFEPYLSP QARPNNAYTA
60 70 80 90 100
MSDSYLPSYY SPSIGFSYSL GEAAWSTGGD TAMPYLTSYG QLSNGEPHFL
110 120 130 140 150
PDAMFGQPGA LGSTPFLGQH GFNFFPSGID FSAWGNNSSQ GQSTQSSGYS
160 170 180 190 200
SNYAYAPSSL GGAMIDGQSA FANETLNKAP GMNTIDQGMA ALKLGSTEVA
210 220 230 240 250
SSVPKVVGSA VGSGSITSNI VASSSLPPAT IAPPKPASWA DIASKPAKQQ
260 270 280 290 300
PKLKTKNGIA GSSLPPPPIK HNMDIGTWDN KGPVAKAPSQ ALVQNIGQPT
310 320 330 340 350
QGSPQPVGQQ ANNSPPVAQA SVGQQTQPLP PPPPQPAQLS VQQQAAQPTR
360 370 380 390 400
WVAPRNRGSG FGHNGVDGNG VGQSQAGSGS TPSEPHPVLE KLRSINNYNP
410 420 430 440 450
KDFDWNLKHG RVFIIKSYSE DDIHRSIKYN IWCSTEHGNK RLDAAYRSMN
460 470 480 490 500
GKGPVYLLFS VNGSGHFCGV AEMKSAVDYN TCAGVWSQDK WKGRFDVRWI
510 520 530 540 550
FVKDVPNSQL RHIRLENNEN KPVTNSRDTQ EVPLEKAKQV LKIIASYKHT
560 570
TSIFDDFSHY EKRQEEEESV KKERQGRGK
Length:579
Mass (Da):62,280
Last modified:December 1, 2001 - v1
Checksum:i9D79DF630593C7AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82A → P in AAH28994 (PubMed:15489334).Curated1
Sequence conflicti165I → V in BAE35173 (PubMed:16141072).Curated1
Sequence conflicti210A → V in BAE35173 (PubMed:16141072).Curated1
Sequence conflicti309Q → R in BAC28785 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031616 mRNA Translation: BAC27480.1
AK034655 mRNA Translation: BAC28785.1
AK083882 mRNA Translation: BAC39048.1
AK150350 mRNA Translation: BAE29487.1
AK150669 mRNA Translation: BAE29751.1
AK151392 mRNA Translation: BAE30361.1
AK151862 mRNA Translation: BAE30751.1
AK159548 mRNA Translation: BAE35173.1
BX537301 Genomic DNA No translation available.
CH466552 Genomic DNA Translation: EDL30120.1
BC014797 mRNA Translation: AAH14797.1
BC028994 mRNA Translation: AAH28994.1
CCDSiCCDS18719.1
RefSeqiNP_663368.3, NM_145393.4
UniGeneiMm.23998
Mm.460463

Genome annotation databases

EnsembliENSMUST00000152796; ENSMUSP00000120414; ENSMUSG00000040025
GeneIDi213541
KEGGimmu:213541
UCSCiuc008vas.2 mouse

Similar proteinsi

Entry informationi

Entry nameiYTHD2_MOUSE
AccessioniPrimary (citable) accession number: Q91YT7
Secondary accession number(s): Q3TWU3, Q8BM70, Q8K325
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health