UniProtKB - Q91YR1 (TWF1_MOUSE)
Twinfilin-1
Twf1
Functioni
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: GO_Central
- actin monomer binding Source: BHF-UCL
- ATP binding Source: MGI
- phosphatidylinositol-4,5-bisphosphate binding Source: BHF-UCL
- protein-containing complex binding Source: MGI
- protein tyrosine kinase activity Source: UniProtKB
GO - Biological processi
- actin filament depolymerization Source: GO_Central
- barbed-end actin filament capping Source: BHF-UCL
- negative regulation of actin filament polymerization Source: BHF-UCL
- positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
- positive regulation of neuron projection development Source: GO_Central
- regulation of actin phosphorylation Source: MGI
- regulation of lamellipodium assembly Source: GO_Central
- sequestering of actin monomers Source: BHF-UCL
Keywordsi
Molecular function | Actin-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Twinfilin-1Alternative name(s): Protein A6 |
Gene namesi | Name:Twf1 Synonyms:Ptk9 |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1100520, Twf1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts.
Cytoskeleton
- actin cytoskeleton Source: UniProtKB
- actin filament Source: GO_Central
Cytosol
- cytosol Source: MGI
Other locations
- cell-cell junction Source: BHF-UCL
- cytoplasm Source: GO_Central
- filopodium Source: BHF-UCL
- myofibril Source: BHF-UCL
- perinuclear region of cytoplasm Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000214951 | 2 – 350 | Twinfilin-1Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 | |
Modified residuei | 309 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 349 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q91YR1 |
jPOSTi | Q91YR1 |
MaxQBi | Q91YR1 |
PaxDbi | Q91YR1 |
PeptideAtlasi | Q91YR1 |
PRIDEi | Q91YR1 |
PTM databases
iPTMneti | Q91YR1 |
PhosphoSitePlusi | Q91YR1 |
SwissPalmi | Q91YR1 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022451, Expressed in utricle of membranous labyrinth and 311 other tissues |
ExpressionAtlasi | Q91YR1, baseline and differential |
Genevisiblei | Q91YR1, MM |
Interactioni
Subunit structurei
Interacts with G-actin; ADP-actin form and capping protein (CP) (PubMed:12807912, PubMed:15282541, PubMed:16511569). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2 (PubMed:12807912). When bound to PI(4,5)P2, it is down-regulated (PubMed:12807912).
Interacts with ACTG1 (By similarity).
By similarity4 PublicationsBinary interactionsi
Q91YR1
With | #Exp. | IntAct |
---|---|---|
ACTA1 [P68135] from Oryctolagus cuniculus. | 2 | EBI-527441,EBI-367540 |
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: GO_Central
- actin monomer binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 202468, 11 interactors |
DIPi | DIP-33923N |
IntActi | Q91YR1, 6 interactors |
MINTi | Q91YR1 |
STRINGi | 10090.ENSMUSP00000023087 |
Miscellaneous databases
RNActi | Q91YR1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q91YR1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q91YR1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 139 | ADF-H 1PROSITE-ProRule annotationAdd BLAST | 138 | |
Domaini | 175 – 313 | ADF-H 2PROSITE-ProRule annotationAdd BLAST | 139 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1747, Eukaryota |
GeneTreei | ENSGT00530000063868 |
HOGENOMi | CLU_031995_1_0_1 |
InParanoidi | Q91YR1 |
OMAi | LFIYTCP |
OrthoDBi | 1578109at2759 |
PhylomeDBi | Q91YR1 |
TreeFami | TF352598 |
Family and domain databases
Gene3Di | 3.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR028458, Twinfilin |
PANTHERi | PTHR13759, PTHR13759, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 2 hits |
SMARTi | View protein in SMART SM00102, ADF, 2 hits |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVVG SCSPPSDSWE
60 70 80 90 100
QDYDSFVLPL LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML
110 120 130 140 150
YAATRATLKK EFGGGHIKDE VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA
160 170 180 190 200
EEELRQIKIN EVQTDVSVDT KHQTLQGVAF PISRDAFQAL EKLSKKQLNY
210 220 230 240 250
VQLEIDIKNE TIILANTENT ELRDLPKRIP KDSARYHFFL YKHSHEGDYL
260 270 280 290 300
ESVVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMD VIRKIEIDNG
310 320 330 340 350
DELTADFLYD EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAEAEATTD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z2H0 | D3Z2H0_MOUSE | Twinfilin-1 | Twf1 | 114 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 345 | A → R in AAB66592 (PubMed:9249064).Curated | 1 | |
Sequence conflicti | 345 | A → R in AAP31404 (PubMed:12807912).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 51 | Q → H1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82324 mRNA Translation: AAB66592.1 AY267188 mRNA Translation: AAP31404.1 AK147990 mRNA Translation: BAE28272.1 AK150852 mRNA Translation: BAE29908.1 BC015081 mRNA Translation: AAH15081.1 BC094034 mRNA Translation: AAH94034.1 |
CCDSi | CCDS27773.1 |
RefSeqi | NP_032997.3, NM_008971.4 |
Genome annotation databases
Ensembli | ENSMUST00000023087; ENSMUSP00000023087; ENSMUSG00000022451 |
GeneIDi | 19230 |
KEGGi | mmu:19230 |
UCSCi | uc007xjl.1, mouse |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Molecular embrace - Issue 73 of August 2006 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82324 mRNA Translation: AAB66592.1 AY267188 mRNA Translation: AAP31404.1 AK147990 mRNA Translation: BAE28272.1 AK150852 mRNA Translation: BAE29908.1 BC015081 mRNA Translation: AAH15081.1 BC094034 mRNA Translation: AAH94034.1 |
CCDSi | CCDS27773.1 |
RefSeqi | NP_032997.3, NM_008971.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M4J | X-ray | 1.60 | A/B | 1-142 | [»] | |
2D8B | NMR | - | A | 161-313 | [»] | |
2HD7 | NMR | - | A | 176-316 | [»] | |
3DAW | X-ray | 2.55 | B | 167-322 | [»] | |
6RSW | X-ray | 1.95 | B | 176-316 | [»] | |
SMRi | Q91YR1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202468, 11 interactors |
DIPi | DIP-33923N |
IntActi | Q91YR1, 6 interactors |
MINTi | Q91YR1 |
STRINGi | 10090.ENSMUSP00000023087 |
PTM databases
iPTMneti | Q91YR1 |
PhosphoSitePlusi | Q91YR1 |
SwissPalmi | Q91YR1 |
Proteomic databases
EPDi | Q91YR1 |
jPOSTi | Q91YR1 |
MaxQBi | Q91YR1 |
PaxDbi | Q91YR1 |
PeptideAtlasi | Q91YR1 |
PRIDEi | Q91YR1 |
Protocols and materials databases
Antibodypediai | 13271, 343 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023087; ENSMUSP00000023087; ENSMUSG00000022451 |
GeneIDi | 19230 |
KEGGi | mmu:19230 |
UCSCi | uc007xjl.1, mouse |
Organism-specific databases
CTDi | 5756 |
MGIi | MGI:1100520, Twf1 |
Phylogenomic databases
eggNOGi | KOG1747, Eukaryota |
GeneTreei | ENSGT00530000063868 |
HOGENOMi | CLU_031995_1_0_1 |
InParanoidi | Q91YR1 |
OMAi | LFIYTCP |
OrthoDBi | 1578109at2759 |
PhylomeDBi | Q91YR1 |
TreeFami | TF352598 |
Miscellaneous databases
BioGRID-ORCSi | 19230, 0 hits in 17 CRISPR screens |
ChiTaRSi | Twf1, mouse |
EvolutionaryTracei | Q91YR1 |
PROi | PR:Q91YR1 |
RNActi | Q91YR1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022451, Expressed in utricle of membranous labyrinth and 311 other tissues |
ExpressionAtlasi | Q91YR1, baseline and differential |
Genevisiblei | Q91YR1, MM |
Family and domain databases
Gene3Di | 3.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR028458, Twinfilin |
PANTHERi | PTHR13759, PTHR13759, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 2 hits |
SMARTi | View protein in SMART SM00102, ADF, 2 hits |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TWF1_MOUSE | |
Accessioni | Q91YR1Primary (citable) accession number: Q91YR1 Secondary accession number(s): O09132, Q52L77, Q80X09 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | April 4, 2006 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries