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Protein

Twinfilin-1

Gene

Twf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.4 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: GO_Central
  • actin monomer binding Source: BHF-UCL
  • ATP binding Source: MGI
  • phosphatidylinositol-4,5-bisphosphate binding Source: BHF-UCL
  • protein-containing complex binding Source: MGI
  • protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-1
Alternative name(s):
Protein A6
Gene namesi
Name:Twf1
Synonyms:Ptk9
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1100520 Twf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002149512 – 350Twinfilin-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei309PhosphotyrosineCombined sources1
Modified residuei349PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on serine and threonine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ91YR1
MaxQBiQ91YR1
PaxDbiQ91YR1
PeptideAtlasiQ91YR1
PRIDEiQ91YR1

PTM databases

iPTMnetiQ91YR1
PhosphoSitePlusiQ91YR1

Expressioni

Tissue specificityi

Widely expressed with highest levels in brain, liver and kidney. Also expressed in heart, lung and testis. Not detected in spleen or skeletal muscle.3 Publications

Developmental stagei

Expression was widespread throughout the embryonic stages analyzed; E10.5, E12.5, E14.5 and E18.5. At E14.5, strongest expression was observed in the developing central and peripheral nervous system (CNS and PNS, respectively) and in the olfactory sensory epithelium. In the CNS, the proliferating neuronal precursors in the ventricular zone expressed it more than the postmitotic neurons. At E18.5, highest expression levels were detected in the mechanosensory hair cells of the inner ear and in the differentiated keratinocytes of the skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000022451 Expressed in 297 organ(s), highest expression level in utricle of membranous labyrinth
ExpressionAtlasiQ91YR1 baseline and differential
GenevisibleiQ91YR1 MM

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form and capping protein (CP) (PubMed:12807912, PubMed:15282541, PubMed:16511569). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2 (PubMed:12807912). When bound to PI(4,5)P2, it is down-regulated (PubMed:12807912). Interacts with ACTG1 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681352EBI-527441,EBI-367540From Oryctolagus cuniculus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202468, 2 interactors
DIPiDIP-33923N
IntActiQ91YR1, 6 interactors
MINTiQ91YR1
STRINGi10090.ENSMUSP00000023087

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ91YR1
SMRiQ91YR1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YR1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 139ADF-H 1PROSITE-ProRule annotationAdd BLAST138
Domaini175 – 313ADF-H 2PROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1747 Eukaryota
ENOG410XS1J LUCA
GeneTreeiENSGT00530000063868
HOGENOMiHOG000168296
HOVERGENiHBG000848
InParanoidiQ91YR1
KOiK08870
OMAiYDYLQFR
OrthoDBiEOG091G0BWK
PhylomeDBiQ91YR1
TreeFamiTF352598

Family and domain databases

Gene3Di3.40.20.10, 2 hits
InterProiView protein in InterPro
IPR002108 ADF-H
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR028458 Twinfilin
PANTHERiPTHR13759 PTHR13759, 1 hit
PfamiView protein in Pfam
PF00241 Cofilin_ADF, 2 hits
SMARTiView protein in SMART
SM00102 ADF, 2 hits
PROSITEiView protein in PROSITE
PS51263 ADF_H, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q91YR1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVVG SCSPPSDSWE
60 70 80 90 100
QDYDSFVLPL LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML
110 120 130 140 150
YAATRATLKK EFGGGHIKDE VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA
160 170 180 190 200
EEELRQIKIN EVQTDVSVDT KHQTLQGVAF PISRDAFQAL EKLSKKQLNY
210 220 230 240 250
VQLEIDIKNE TIILANTENT ELRDLPKRIP KDSARYHFFL YKHSHEGDYL
260 270 280 290 300
ESVVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMD VIRKIEIDNG
310 320 330 340 350
DELTADFLYD EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAEAEATTD
Length:350
Mass (Da):40,079
Last modified:April 4, 2006 - v2
Checksum:i643CBBA18D38D4E0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z2H0D3Z2H0_MOUSE
Twinfilin-1
Twf1
114Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti345A → R in AAB66592 (PubMed:9249064).Curated1
Sequence conflicti345A → R in AAP31404 (PubMed:12807912).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti51Q → H1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82324 mRNA Translation: AAB66592.1
AY267188 mRNA Translation: AAP31404.1
AK147990 mRNA Translation: BAE28272.1
AK150852 mRNA Translation: BAE29908.1
BC015081 mRNA Translation: AAH15081.1
BC094034 mRNA Translation: AAH94034.1
CCDSiCCDS27773.1
RefSeqiNP_032997.3, NM_008971.4
UniGeneiMm.490250

Genome annotation databases

EnsembliENSMUST00000023087; ENSMUSP00000023087; ENSMUSG00000022451
GeneIDi19230
KEGGimmu:19230
UCSCiuc007xjl.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82324 mRNA Translation: AAB66592.1
AY267188 mRNA Translation: AAP31404.1
AK147990 mRNA Translation: BAE28272.1
AK150852 mRNA Translation: BAE29908.1
BC015081 mRNA Translation: AAH15081.1
BC094034 mRNA Translation: AAH94034.1
CCDSiCCDS27773.1
RefSeqiNP_032997.3, NM_008971.4
UniGeneiMm.490250

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M4JX-ray1.60A/B1-142[»]
2D8BNMR-A161-313[»]
2HD7NMR-A176-316[»]
3DAWX-ray2.55B167-322[»]
ProteinModelPortaliQ91YR1
SMRiQ91YR1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202468, 2 interactors
DIPiDIP-33923N
IntActiQ91YR1, 6 interactors
MINTiQ91YR1
STRINGi10090.ENSMUSP00000023087

PTM databases

iPTMnetiQ91YR1
PhosphoSitePlusiQ91YR1

Proteomic databases

EPDiQ91YR1
MaxQBiQ91YR1
PaxDbiQ91YR1
PeptideAtlasiQ91YR1
PRIDEiQ91YR1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023087; ENSMUSP00000023087; ENSMUSG00000022451
GeneIDi19230
KEGGimmu:19230
UCSCiuc007xjl.1 mouse

Organism-specific databases

CTDi5756
MGIiMGI:1100520 Twf1

Phylogenomic databases

eggNOGiKOG1747 Eukaryota
ENOG410XS1J LUCA
GeneTreeiENSGT00530000063868
HOGENOMiHOG000168296
HOVERGENiHBG000848
InParanoidiQ91YR1
KOiK08870
OMAiYDYLQFR
OrthoDBiEOG091G0BWK
PhylomeDBiQ91YR1
TreeFamiTF352598

Miscellaneous databases

ChiTaRSiTwf1 mouse
EvolutionaryTraceiQ91YR1
PROiPR:Q91YR1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022451 Expressed in 297 organ(s), highest expression level in utricle of membranous labyrinth
ExpressionAtlasiQ91YR1 baseline and differential
GenevisibleiQ91YR1 MM

Family and domain databases

Gene3Di3.40.20.10, 2 hits
InterProiView protein in InterPro
IPR002108 ADF-H
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR028458 Twinfilin
PANTHERiPTHR13759 PTHR13759, 1 hit
PfamiView protein in Pfam
PF00241 Cofilin_ADF, 2 hits
SMARTiView protein in SMART
SM00102 ADF, 2 hits
PROSITEiView protein in PROSITE
PS51263 ADF_H, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTWF1_MOUSE
AccessioniPrimary (citable) accession number: Q91YR1
Secondary accession number(s): O09132, Q52L77, Q80X09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: April 4, 2006
Last modified: November 7, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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Main funding by: National Institutes of Health

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