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Protein

Argininosuccinate lyase

Gene

Asl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.

Activity regulationi

Enzyme activity is regulated by acetylation.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (Ass1)
  3. Argininosuccinate lyase (Asl)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-arginine and fumarate from (N(omega)-L-arginino)succinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate lyase (Asl)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine and fumarate from (N(omega)-L-arginino)succinate, the pathway urea cycle and in Nitrogen metabolism.

GO - Molecular functioni

GO - Biological processi

  • ammonia assimilation cycle Source: MGI
  • arginine biosynthetic process via ornithine Source: GO_Central
  • argininosuccinate metabolic process Source: MGI
  • cellular amino acid metabolic process Source: MGI
  • liver development Source: MGI
  • locomotory behavior Source: MGI
  • post-embryonic development Source: MGI
  • urea cycle Source: MGI

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Enzyme and pathway databases

ReactomeiR-MMU-70635 Urea cycle
UniPathwayi
UPA00068;UER00114

UPA00158;UER00273

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate lyase (EC:4.3.2.1)
Short name:
ASAL
Alternative name(s):
Arginosuccinase
Gene namesi
Name:Asl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88084 Asl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001377142 – 464Argininosuccinate lyaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7N6-acetyllysineCombined sources1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei288N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TCA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91YI0
MaxQBiQ91YI0
PaxDbiQ91YI0
PRIDEiQ91YI0

PTM databases

CarbonylDBiQ91YI0
iPTMnetiQ91YI0
PhosphoSitePlusiQ91YI0

Expressioni

Gene expression databases

BgeeiENSMUSG00000025533 Expressed in 298 organ(s), highest expression level in liver
CleanExiMM_ASL
ExpressionAtlasiQ91YI0 baseline and differential
GenevisibleiQ91YI0 MM

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi225136, 1 interactor
IntActiQ91YI0, 2 interactors
MINTiQ91YI0
STRINGi10090.ENSMUSP00000123799

Structurei

3D structure databases

ProteinModelPortaliQ91YI0
SMRiQ91YI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1316 Eukaryota
COG0165 LUCA
GeneTreeiENSGT00390000014045
HOGENOMiHOG000242744
HOVERGENiHBG004281
InParanoidiQ91YI0
KOiK01755
OMAiKKNPDVF
OrthoDBiEOG091G0AJA
PhylomeDBiQ91YI0
TreeFamiTF300656

Family and domain databases

CDDicd01359 Argininosuccinate_lyase, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00006 Arg_succ_lyase, 1 hit
InterProiView protein in InterPro
IPR029419 Arg_succ_lyase_C
IPR009049 Argininosuccinate_lyase
IPR024083 Fumarase/histidase_N
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR43814 PTHR43814, 1 hit
PfamiView protein in Pfam
PF14698 ASL_C2, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00838 argH, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q91YI0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASESGKLWG GRFVGAVDPI MEKFNSSISY DRHLWNVDVQ GSKAYSRGLE
60 70 80 90 100
KAGLLTKAEM QQILQGLDKV AEEWAQGTFK LHPNDEDIHT ANERRLKELI
110 120 130 140 150
GEAAGKLHTG RSRNDQVVTD LRLWMRQTCS KLSALLRVLI GTMVDRAEAE
160 170 180 190 200
RDVLFPGYTH LQRAQPIRWS HWILSHAVAL TRDSERLLEV QKRINVLPLG
210 220 230 240 250
SGAIAGNPLG VDRELLRAEL NFGAITLNSM DATSERDFVA EFLFWASLCM
260 270 280 290 300
THLSRMAEDL ILYGTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA
310 320 330 340 350
GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMI AVLQVATGVI
360 370 380 390 400
STLQIHRENM KQALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET
410 420 430 440 450
KGVALNLLSL QELQTISPLF SGDVSHVWDY SHSVEQYSAL GGTAKSSVEW
460
QIRQVRALLQ AQEP
Length:464
Mass (Da):51,739
Last modified:December 1, 2001 - v1
Checksum:iA78ABC52A9285A59
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CY49E0CY49_MOUSE
Argininosuccinate lyase
Asl
262Annotation score:
F7D439F7D439_MOUSE
Argininosuccinate lyase
Asl
267Annotation score:
E0CYV3E0CYV3_MOUSE
Argininosuccinate lyase
Asl
74Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076453 mRNA Translation: BAC36348.1
AK140160 mRNA Translation: BAE24262.1
AK148764 mRNA Translation: BAE28659.1
AK168739 mRNA Translation: BAE40580.1
BC016670 mRNA Translation: AAH16670.1
CCDSiCCDS51655.1
RefSeqiNP_598529.1, NM_133768.5
XP_006504404.1, XM_006504341.3
UniGeneiMm.23869

Genome annotation databases

EnsembliENSMUST00000159619; ENSMUSP00000123799; ENSMUSG00000025533
ENSMUST00000160129; ENSMUSP00000124579; ENSMUSG00000025533
ENSMUST00000161094; ENSMUSP00000124274; ENSMUSG00000025533
GeneIDi109900
KEGGimmu:109900
UCSCiuc008zty.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076453 mRNA Translation: BAC36348.1
AK140160 mRNA Translation: BAE24262.1
AK148764 mRNA Translation: BAE28659.1
AK168739 mRNA Translation: BAE40580.1
BC016670 mRNA Translation: AAH16670.1
CCDSiCCDS51655.1
RefSeqiNP_598529.1, NM_133768.5
XP_006504404.1, XM_006504341.3
UniGeneiMm.23869

3D structure databases

ProteinModelPortaliQ91YI0
SMRiQ91YI0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225136, 1 interactor
IntActiQ91YI0, 2 interactors
MINTiQ91YI0
STRINGi10090.ENSMUSP00000123799

PTM databases

CarbonylDBiQ91YI0
iPTMnetiQ91YI0
PhosphoSitePlusiQ91YI0

Proteomic databases

EPDiQ91YI0
MaxQBiQ91YI0
PaxDbiQ91YI0
PRIDEiQ91YI0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000159619; ENSMUSP00000123799; ENSMUSG00000025533
ENSMUST00000160129; ENSMUSP00000124579; ENSMUSG00000025533
ENSMUST00000161094; ENSMUSP00000124274; ENSMUSG00000025533
GeneIDi109900
KEGGimmu:109900
UCSCiuc008zty.1 mouse

Organism-specific databases

CTDi435
MGIiMGI:88084 Asl

Phylogenomic databases

eggNOGiKOG1316 Eukaryota
COG0165 LUCA
GeneTreeiENSGT00390000014045
HOGENOMiHOG000242744
HOVERGENiHBG004281
InParanoidiQ91YI0
KOiK01755
OMAiKKNPDVF
OrthoDBiEOG091G0AJA
PhylomeDBiQ91YI0
TreeFamiTF300656

Enzyme and pathway databases

UniPathwayi
UPA00068;UER00114

UPA00158;UER00273

ReactomeiR-MMU-70635 Urea cycle

Miscellaneous databases

PROiPR:Q91YI0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025533 Expressed in 298 organ(s), highest expression level in liver
CleanExiMM_ASL
ExpressionAtlasiQ91YI0 baseline and differential
GenevisibleiQ91YI0 MM

Family and domain databases

CDDicd01359 Argininosuccinate_lyase, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00006 Arg_succ_lyase, 1 hit
InterProiView protein in InterPro
IPR029419 Arg_succ_lyase_C
IPR009049 Argininosuccinate_lyase
IPR024083 Fumarase/histidase_N
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR43814 PTHR43814, 1 hit
PfamiView protein in Pfam
PF14698 ASL_C2, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00838 argH, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARLY_MOUSE
AccessioniPrimary (citable) accession number: Q91YI0
Secondary accession number(s): Q3UFA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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