UniProtKB - Q91YE9 (5NT1B_MOUSE)

BLAST

>sp|Q91YE9|5NT1B_MOUSE Cytosolic 5'-nucleotidase 1B OS=Mus musculus OX=10090 GN=Nt5c1b PE=2 SV=2
MSQTSLKHKKKNEPGMRYSKESLDAEKRKDSDKTGARLSTQGSQELPLHNTDSRGYVVRN
QWSRTSRSPSTGAPSVDEPRSRNTAIKVEAPNSSTTSRTSSASPSQHETSPPPQTSEKSS
IQQTPQNRPITQLESQPPTPPETEPNSRRTSAKMYTGSDPWAHRENREPRDLQLRDYAYS
CDSREGMPKTREYPRTPPTEWKPYAQRRLQYGTSVDMEPEYISDGPQQRQRQQTEEDEVD
EAYWTSVSMLYEKIPSCARPRPPKPKHAITIAVSSRALFNMVDDRKIYEEEGLEKYMEYQ
LTNENVILTPGPAFRFVKALQHVNSRLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNH
YGLLIDRFCLTGGKSPIGYLKAYLTNLYLSADSEKVQEAIKEGIASATMYAGAKDMAYCD
TQLRVAFDGDAVLFSDESEHIAKDHGLDKFFQHETLFENKPLAQGPLKSFLEDLGKLQKK
FYAKDERLLCPIRTYLVTARSAASSGARVLKTLRRWGLEIDEALFLAGAPKGPILVKIRP
HIFFDDQMFHIESAQKFGTITAHVPYGIAQKRN

Align

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History
Entry version 113 (07 Nov 2018)
Sequence version 2 (28 Jun 2011)
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Protein

Cytosolic 5'-nucleotidase 1B

Gene

Nt5c1b

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at transcript levelⁱ

Functionⁱ

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.1 Publication

Catalytic activityⁱ

A 5'-ribonucleotide + H₂O = a ribonucleoside + phosphate.

Cofactorⁱ

Mg²⁺

Activity regulationⁱ

Activated by ADP.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	428	NucleophileBy similarity		1

GO - Molecular functionⁱ

5'-nucleotidase activity
Source: MGI
Inferred from direct assayⁱ
- 11690631
magnesium ion binding Source: InterPro
nucleotide binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenosine metabolic process Source: MGI
nucleoside metabolic process
Source: MGI
Inferred from direct assayⁱ
- 11690631
nucleotide metabolic process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Nucleotide metabolism
Ligand	Magnesium

Enzyme and pathway databases

Reactomeⁱ	R-MMU-74259 Purine catabolism
SABIO-RKⁱ	Q91YE9

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5) Short name: cN1B Alternative name(s): Autoimmune infertility-related protein Cytosolic 5'-nucleotidase IB Short name: cN-IB
Gene namesⁱ	Name:Nt5c1b Synonyms:Airp
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 12

Organism-specific databases

MGIⁱ	MGI:1918131 Nt5c1b

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000144797	1 – 573	Cytosolic 5'-nucleotidase 1BAdd BLAST		573

Proteomic databases

PaxDbⁱ	Q91YE9
PRIDEⁱ	Q91YE9

PTM databases

iPTMnetⁱ	Q91YE9
PhosphoSitePlusⁱ	Q91YE9

Expressionⁱ

Tissue specificityⁱ

Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000020622 Expressed in 20 organ(s), highest expression level in seminiferous tubule of testis
CleanExⁱ	MM_NT5C1B
ExpressionAtlasⁱ	Q91YE9 baseline and differential
Genevisibleⁱ	Q91YE9 MM

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000002456

STRINGⁱ

10090.ENSMUSP00000002456

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q91YE9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410IE01 Eukaryota ENOG410XPRD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017767
HOGENOMⁱ	HOG000243802
HOVERGENⁱ	HBG050426
InParanoidⁱ	Q91YE9
KEGG Orthology (KO) More...KOⁱ	K01081
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EZE
TreeFamⁱ	TF329831

Family and domain databases

InterProⁱ	View protein in InterPro IPR010394 5-nucleotidase
PANTHERⁱ	PTHR31367 PTHR31367, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06189 5-nucleotidase, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 17 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q91YE9-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN 
        60         70         80         90        100
TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS 
       110        120        130        140        150
SASPSQHETS PPPQTSEKSS IQQTPQNRPI TQLESQPPTP PETEPNSRRT 
       160        170        180        190        200
SAKMYTGSDP WAHRENREPR DLQLRDYAYS CDSREGMPKT REYPRTPPTE 
       210        220        230        240        250
WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD EAYWTSVSML 
       260        270        280        290        300
YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ 
       310        320        330        340        350
LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV 
       360        370        380        390        400
GVRLINSVNH YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI 
       410        420        430        440        450
KEGIASATMY AGAKDMAYCD TQLRVAFDGD AVLFSDESEH IAKDHGLDKF 
       460        470        480        490        500
FQHETLFENK PLAQGPLKSF LEDLGKLQKK FYAKDERLLC PIRTYLVTAR 
       510        520        530        540        550
SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP HIFFDDQMFH 
       560        570 
IESAQKFGTI TAHVPYGIAQ KRN

Length:573

Mass (Da):65,002

Last modified:June 28, 2011 - v2

Checksum:ⁱ948868F505D6B965

Isoform 2 (identifier: Q91YE9-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
88-89: Missing.
247-262: Missing.

« Hide

        10         20         30         40         50
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN 
        60         70         80         90        100
TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKAPN SSTTSRTSSA 
       110        120        130        140        150
SPSQHETSPP PQTSEKSSIQ QTPQNRPITQ LESQPPTPPE TEPNSRRTSA 
       160        170        180        190        200
KMYTGSDPWA HRENREPRDL QLRDYAYSCD SREGMPKTRE YPRTPPTEWK 
       210        220        230        240        250
PYAQRRLQYG TSVDMEPEYI SDGPQQRQRQ QTEEDEVDEA YWTSPKPKHA 
       260        270        280        290        300
ITIAVSSRAL FNMVDDRKIY EEEGLEKYME YQLTNENVIL TPGPAFRFVK 
       310        320        330        340        350
ALQHVNSRLR DLYPDEQDLF DIVLMTNNHA QVGVRLINSV NHYGLLIDRF 
       360        370        380        390        400
CLTGGKSPIG YLKAYLTNLY LSADSEKVQE AIKEGIASAT MYAGAKDMAY 
       410        420        430        440        450
CDTQLRVAFD GDAVLFSDES EHIAKDHGLD KFFQHETLFE NKPLAQGPLK 
       460        470        480        490        500
SFLEDLGKLQ KKFYAKDERL LCPIRTYLVT ARSAASSGAR VLKTLRRWGL 
       510        520        530        540        550
EIDEALFLAG APKGPILVKI RPHIFFDDQM FHIESAQKFG TITAHVPYGI 

AQKRN

Note: No experimental confirmation available.

Show »

Length:555

Mass (Da):62,945

Checksum:ⁱ99417F747816E36C

Computationally mapped potential isoform sequencesⁱ

There are 17 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name	Protein names	Gene names	Length	Annotation

	A0A1W2P7K9	A0A1W2P7K9_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	615	Annotation score:
	A0A1W2P6X8	A0A1W2P6X8_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	631	Annotation score:
	A0A1W2P7E9	A0A1W2P7E9_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	571	Annotation score:
	D6REF4	D6REF4_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	474	Annotation score:
	D3Z145	D3Z145_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	271	Annotation score:
	A0A1Y7VJB2	A0A1Y7VJB2_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	452	Annotation score:
	A0A1W2P782	A0A1W2P782_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	430	Annotation score:
	A0A1Y7VK90	A0A1Y7VK90_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	557	Annotation score:
	A0A1W2P7S7	A0A1W2P7S7_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	495	Annotation score:
	A0A1W2P7V4	A0A1W2P7V4_MOUSE	Cytosolic 5'-nucleotidase 1B Cytosolic 5'-nucleotidase 1B	Nt5c1b	391	Annotation score:
There are more potential isoformsShow all

Sequence cautionⁱ

The sequence AAK39109 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	103	S → F in AAK39109 (Ref. 2) Curated	1
Sequence conflictⁱ	121	I → T in CAC44364 (PubMed:11690631).Curated	1
Sequence conflictⁱ	121	I → T in AAK39109 (Ref. 2) Curated	1
Sequence conflictⁱ	162	A → V in CAC44364 (PubMed:11690631).Curated	1
Sequence conflictⁱ	185	E → Q in CAC44364 (PubMed:11690631).Curated	1
Sequence conflictⁱ	228	Q → H in CAC44364 (PubMed:11690631).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_010204	88 – 89	Missing in isoform 2. 1 Publication		2
Alternative sequenceⁱVSP_010205	247 – 262	Missing in isoform 2. 1 PublicationAdd BLAST		16

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ295253 mRNA Translation: CAC44364.1 AF356186 mRNA Translation: AAK39109.1 Different initiation. AC157570 Genomic DNA No translation available.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25810.1 [Q91YE9-1] CCDS79106.1 [Q91YE9-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_081864.2, NM_027588.3 [Q91YE9-1]
UniGeneⁱ	Mm.272843

Genome annotation databases

Ensemblⁱ	ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1] ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2]
GeneIDⁱ	70881
KEGGⁱ	mmu:70881
UCSC genome browser More...UCSCⁱ	uc007nap.2 mouse [Q91YE9-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q91YE9	Cytosolic 5'-nucleotidase 1B		MOUSE		615	UniRef90_Q91YE9
UPI000A31A1A4		MUSCR		615
UPI000A30B288		MUSPA		555
Cytosolic 5'-nucleotidase 1B		MOUSE		571
Cytosolic 5'-nucleotidase 1B		MOUSE		557
+6

Protein	Similar proteins		Species	Score	Length	Source
Q91YE9	Cytosolic 5'-nucleotidase 1B		HUMAN		610	UniRef50_Q91YE9
Cytosolic 5'-nucleotidase 1B		MOUSE		615
cytosolic 5'-nucleotidase 1B-like isoform X2		ERIEU		625
Uncharacterized protein		PROCO		546
Cytosolic 5'-nucleotidase 1B (Fragment)		TUPCH		630
+343

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ295253 mRNA Translation: CAC44364.1 AF356186 mRNA Translation: AAK39109.1 Different initiation. AC157570 Genomic DNA No translation available.
CCDSⁱ	CCDS25810.1 [Q91YE9-1] CCDS79106.1 [Q91YE9-2]
RefSeqⁱ	NP_081864.2, NM_027588.3 [Q91YE9-1]
UniGeneⁱ	Mm.272843

3D structure databases

ProteinModelPortalⁱ	Q91YE9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000002456

STRINGⁱ

10090.ENSMUSP00000002456

PTM databases

iPTMnetⁱ	Q91YE9
PhosphoSitePlusⁱ	Q91YE9

Proteomic databases

PaxDbⁱ	Q91YE9
PRIDEⁱ	Q91YE9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1] ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2]
GeneIDⁱ	70881
KEGGⁱ	mmu:70881
UCSCⁱ	uc007nap.2 mouse [Q91YE9-1]

Organism-specific databases

CTDⁱ	93034
MGIⁱ	MGI:1918131 Nt5c1b

Phylogenomic databases

eggNOGⁱ	ENOG410IE01 Eukaryota ENOG410XPRD LUCA
GeneTreeⁱ	ENSGT00390000017767
HOGENOMⁱ	HOG000243802
HOVERGENⁱ	HBG050426
InParanoidⁱ	Q91YE9
KOⁱ	K01081
OrthoDBⁱ	EOG091G0EZE
TreeFamⁱ	TF329831

Enzyme and pathway databases

Reactomeⁱ	R-MMU-74259 Purine catabolism
SABIO-RKⁱ	Q91YE9

Miscellaneous databases

ChiTaRSⁱ	Nt5c1b mouse
Protein Ontology More...PROⁱ	PR:Q91YE9
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000020622 Expressed in 20 organ(s), highest expression level in seminiferous tubule of testis
CleanExⁱ	MM_NT5C1B
ExpressionAtlasⁱ	Q91YE9 baseline and differential
Genevisibleⁱ	Q91YE9 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR010394 5-nucleotidase
PANTHERⁱ	PTHR31367 PTHR31367, 1 hit
Pfamⁱ	View protein in Pfam PF06189 5-nucleotidase, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	5NT1B_MOUSE
Accessionⁱ	Q91YE9Primary (citable) accession number: Q91YE9 Secondary accession number(s): E9QNH2, Q91Y48
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
	Last sequence update:	June 28, 2011
	Last modified:	November 7, 2018
	This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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Supporting data

UniProtKB - Q91YE9 (5NT1B_MOUSE)

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Cytosolic 5'-nucleotidase 1B

Nt5c1b

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ