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Protein

Cytosolic 5'-nucleotidase 1B

Gene

Nt5c1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Activity regulationi

Activated by ADP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei428NucleophileBy similarity1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: MGI
  • magnesium ion binding Source: InterPro
  • nucleotide binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-MMU-74259 Purine catabolism
SABIO-RKiQ91YE9

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)
Short name:
cN1B
Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name:
cN-IB
Gene namesi
Name:Nt5c1b
Synonyms:Airp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1918131 Nt5c1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447971 – 573Cytosolic 5'-nucleotidase 1BAdd BLAST573

Proteomic databases

PaxDbiQ91YE9
PRIDEiQ91YE9

PTM databases

iPTMnetiQ91YE9
PhosphoSitePlusiQ91YE9

Expressioni

Tissue specificityi

Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000020622 Expressed in 20 organ(s), highest expression level in seminiferous tubule of testis
CleanExiMM_NT5C1B
ExpressionAtlasiQ91YE9 baseline and differential
GenevisibleiQ91YE9 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002456

Structurei

3D structure databases

ProteinModelPortaliQ91YE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGiENOG410IE01 Eukaryota
ENOG410XPRD LUCA
GeneTreeiENSGT00390000017767
HOGENOMiHOG000243802
HOVERGENiHBG050426
InParanoidiQ91YE9
KOiK01081
OMAiPKPKHAI
OrthoDBiEOG091G0EZE
TreeFamiTF329831

Family and domain databases

InterProiView protein in InterPro
IPR010394 5-nucleotidase
PANTHERiPTHR31367 PTHR31367, 1 hit
PfamiView protein in Pfam
PF06189 5-nucleotidase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 17 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91YE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN
60 70 80 90 100
TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS
110 120 130 140 150
SASPSQHETS PPPQTSEKSS IQQTPQNRPI TQLESQPPTP PETEPNSRRT
160 170 180 190 200
SAKMYTGSDP WAHRENREPR DLQLRDYAYS CDSREGMPKT REYPRTPPTE
210 220 230 240 250
WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD EAYWTSVSML
260 270 280 290 300
YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ
310 320 330 340 350
LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV
360 370 380 390 400
GVRLINSVNH YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI
410 420 430 440 450
KEGIASATMY AGAKDMAYCD TQLRVAFDGD AVLFSDESEH IAKDHGLDKF
460 470 480 490 500
FQHETLFENK PLAQGPLKSF LEDLGKLQKK FYAKDERLLC PIRTYLVTAR
510 520 530 540 550
SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP HIFFDDQMFH
560 570
IESAQKFGTI TAHVPYGIAQ KRN
Length:573
Mass (Da):65,002
Last modified:June 28, 2011 - v2
Checksum:i948868F505D6B965
GO
Isoform 2 (identifier: Q91YE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: Missing.
     247-262: Missing.

Note: No experimental confirmation available.
Show »
Length:555
Mass (Da):62,945
Checksum:i99417F747816E36C
GO

Computationally mapped potential isoform sequencesi

There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P6X8A0A1W2P6X8_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
631Annotation score:
A0A1W2P7K9A0A1W2P7K9_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
615Annotation score:
A0A1W2P7E9A0A1W2P7E9_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
571Annotation score:
D6REF4D6REF4_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
474Annotation score:
D3Z145D3Z145_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
271Annotation score:
A0A1Y7VK90A0A1Y7VK90_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
557Annotation score:
A0A1Y7VJB2A0A1Y7VJB2_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
452Annotation score:
A0A1W2P782A0A1W2P782_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
430Annotation score:
A0A1W2P7V4A0A1W2P7V4_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
391Annotation score:
A0A1W2P7S7A0A1W2P7S7_MOUSE
Cytosolic 5'-nucleotidase 1B
Nt5c1b
495Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence AAK39109 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103S → F in AAK39109 (Ref. 2) Curated1
Sequence conflicti121I → T in CAC44364 (PubMed:11690631).Curated1
Sequence conflicti121I → T in AAK39109 (Ref. 2) Curated1
Sequence conflicti162A → V in CAC44364 (PubMed:11690631).Curated1
Sequence conflicti185E → Q in CAC44364 (PubMed:11690631).Curated1
Sequence conflicti228Q → H in CAC44364 (PubMed:11690631).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01020488 – 89Missing in isoform 2. 1 Publication2
Alternative sequenceiVSP_010205247 – 262Missing in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295253 mRNA Translation: CAC44364.1
AF356186 mRNA Translation: AAK39109.1 Different initiation.
AC157570 Genomic DNA No translation available.
CCDSiCCDS25810.1 [Q91YE9-1]
CCDS79106.1 [Q91YE9-2]
RefSeqiNP_081864.2, NM_027588.3 [Q91YE9-1]
UniGeneiMm.272843

Genome annotation databases

EnsembliENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1]
ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2]
GeneIDi70881
KEGGimmu:70881
UCSCiuc007nap.2 mouse [Q91YE9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295253 mRNA Translation: CAC44364.1
AF356186 mRNA Translation: AAK39109.1 Different initiation.
AC157570 Genomic DNA No translation available.
CCDSiCCDS25810.1 [Q91YE9-1]
CCDS79106.1 [Q91YE9-2]
RefSeqiNP_081864.2, NM_027588.3 [Q91YE9-1]
UniGeneiMm.272843

3D structure databases

ProteinModelPortaliQ91YE9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002456

PTM databases

iPTMnetiQ91YE9
PhosphoSitePlusiQ91YE9

Proteomic databases

PaxDbiQ91YE9
PRIDEiQ91YE9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1]
ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2]
GeneIDi70881
KEGGimmu:70881
UCSCiuc007nap.2 mouse [Q91YE9-1]

Organism-specific databases

CTDi93034
MGIiMGI:1918131 Nt5c1b

Phylogenomic databases

eggNOGiENOG410IE01 Eukaryota
ENOG410XPRD LUCA
GeneTreeiENSGT00390000017767
HOGENOMiHOG000243802
HOVERGENiHBG050426
InParanoidiQ91YE9
KOiK01081
OMAiPKPKHAI
OrthoDBiEOG091G0EZE
TreeFamiTF329831

Enzyme and pathway databases

ReactomeiR-MMU-74259 Purine catabolism
SABIO-RKiQ91YE9

Miscellaneous databases

ChiTaRSiNt5c1b mouse
PROiPR:Q91YE9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020622 Expressed in 20 organ(s), highest expression level in seminiferous tubule of testis
CleanExiMM_NT5C1B
ExpressionAtlasiQ91YE9 baseline and differential
GenevisibleiQ91YE9 MM

Family and domain databases

InterProiView protein in InterPro
IPR010394 5-nucleotidase
PANTHERiPTHR31367 PTHR31367, 1 hit
PfamiView protein in Pfam
PF06189 5-nucleotidase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5NT1B_MOUSE
AccessioniPrimary (citable) accession number: Q91YE9
Secondary accession number(s): E9QNH2, Q91Y48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 28, 2011
Last modified: September 12, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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