UniProtKB - Q91YE9 (5NT1B_MOUSE)
Protein
Cytosolic 5'-nucleotidase 1B
Gene
Nt5c1b
Organism
Mus musculus (Mouse)
Status
Functioni
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.1 Publication
Catalytic activityi
- EC:3.1.3.5
Cofactori
Activity regulationi
Activated by ADP.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 428 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- 5'-nucleotidase activity Source: MGI
- magnesium ion binding Source: InterPro
- nucleotide binding Source: InterPro
GO - Biological processi
- adenosine metabolic process Source: MGI
- nucleoside metabolic process Source: MGI
- nucleotide metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium |
Enzyme and pathway databases
Reactomei | R-MMU-74259, Purine catabolism |
SABIO-RKi | Q91YE9 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)Short name: cN1B Alternative name(s): Autoimmune infertility-related protein Cytosolic 5'-nucleotidase IB Short name: cN-IB |
Gene namesi | Name:Nt5c1b Synonyms:Airp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918131, Nt5c1b |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144797 | 1 – 573 | Cytosolic 5'-nucleotidase 1BAdd BLAST | 573 |
Proteomic databases
PaxDbi | Q91YE9 |
PRIDEi | Q91YE9 |
PTM databases
iPTMneti | Q91YE9 |
PhosphoSitePlusi | Q91YE9 |
Expressioni
Tissue specificityi
Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000020622, Expressed in spermatid and 44 other tissues |
ExpressionAtlasi | Q91YE9, baseline and differential |
Genevisiblei | Q91YE9, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000002456 |
Miscellaneous databases
RNActi | Q91YE9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the 5'-nucleotidase type 3 family.Curated
Phylogenomic databases
eggNOGi | ENOG502QRJF, Eukaryota |
GeneTreei | ENSGT00390000017767 |
HOGENOMi | CLU_035579_0_0_1 |
InParanoidi | Q91YE9 |
TreeFami | TF329831 |
Family and domain databases
InterProi | View protein in InterPro IPR010394, 5-nucleotidase |
PANTHERi | PTHR31367, PTHR31367, 1 hit |
Pfami | View protein in Pfam PF06189, 5-nucleotidase, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 17 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q91YE9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN
60 70 80 90 100
TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS
110 120 130 140 150
SASPSQHETS PPPQTSEKSS IQQTPQNRPI TQLESQPPTP PETEPNSRRT
160 170 180 190 200
SAKMYTGSDP WAHRENREPR DLQLRDYAYS CDSREGMPKT REYPRTPPTE
210 220 230 240 250
WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD EAYWTSVSML
260 270 280 290 300
YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ
310 320 330 340 350
LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV
360 370 380 390 400
GVRLINSVNH YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI
410 420 430 440 450
KEGIASATMY AGAKDMAYCD TQLRVAFDGD AVLFSDESEH IAKDHGLDKF
460 470 480 490 500
FQHETLFENK PLAQGPLKSF LEDLGKLQKK FYAKDERLLC PIRTYLVTAR
510 520 530 540 550
SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP HIFFDDQMFH
560 570
IESAQKFGTI TAHVPYGIAQ KRN
Computationally mapped potential isoform sequencesi
There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P6X8 | A0A1W2P6X8_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 631 | Annotation score: | ||
A0A1W2P7K9 | A0A1W2P7K9_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 615 | Annotation score: | ||
A0A1W2P7E9 | A0A1W2P7E9_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 571 | Annotation score: | ||
A0A1W2P782 | A0A1W2P782_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 430 | Annotation score: | ||
A0A1Y7VK90 | A0A1Y7VK90_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 557 | Annotation score: | ||
D3Z145 | D3Z145_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 271 | Annotation score: | ||
D6REF4 | D6REF4_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 474 | Annotation score: | ||
A0A1W2P7S7 | A0A1W2P7S7_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 495 | Annotation score: | ||
A0A1W2P7V4 | A0A1W2P7V4_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 391 | Annotation score: | ||
A0A1Y7VJB2 | A0A1Y7VJB2_MOUSE | Cytosolic 5'-nucleotidase 1B | Nt5c1b | 452 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
The sequence AAK39109 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | S → F in AAK39109 (Ref. 2) Curated | 1 | |
Sequence conflicti | 121 | I → T in CAC44364 (PubMed:11690631).Curated | 1 | |
Sequence conflicti | 121 | I → T in AAK39109 (Ref. 2) Curated | 1 | |
Sequence conflicti | 162 | A → V in CAC44364 (PubMed:11690631).Curated | 1 | |
Sequence conflicti | 185 | E → Q in CAC44364 (PubMed:11690631).Curated | 1 | |
Sequence conflicti | 228 | Q → H in CAC44364 (PubMed:11690631).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010204 | 88 – 89 | Missing in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_010205 | 247 – 262 | Missing in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ295253 mRNA Translation: CAC44364.1 AF356186 mRNA Translation: AAK39109.1 Different initiation. AC157570 Genomic DNA No translation available. |
CCDSi | CCDS25810.1 [Q91YE9-1] CCDS79106.1 [Q91YE9-2] |
RefSeqi | NP_081864.2, NM_027588.3 [Q91YE9-1] |
Genome annotation databases
Ensembli | ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1] ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2] |
GeneIDi | 70881 |
KEGGi | mmu:70881 |
UCSCi | uc007nap.2, mouse [Q91YE9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ295253 mRNA Translation: CAC44364.1 AF356186 mRNA Translation: AAK39109.1 Different initiation. AC157570 Genomic DNA No translation available. |
CCDSi | CCDS25810.1 [Q91YE9-1] CCDS79106.1 [Q91YE9-2] |
RefSeqi | NP_081864.2, NM_027588.3 [Q91YE9-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000002456 |
PTM databases
iPTMneti | Q91YE9 |
PhosphoSitePlusi | Q91YE9 |
Proteomic databases
PaxDbi | Q91YE9 |
PRIDEi | Q91YE9 |
Genome annotation databases
Ensembli | ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622 [Q91YE9-1] ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622 [Q91YE9-2] |
GeneIDi | 70881 |
KEGGi | mmu:70881 |
UCSCi | uc007nap.2, mouse [Q91YE9-1] |
Organism-specific databases
CTDi | 93034 |
MGIi | MGI:1918131, Nt5c1b |
Phylogenomic databases
eggNOGi | ENOG502QRJF, Eukaryota |
GeneTreei | ENSGT00390000017767 |
HOGENOMi | CLU_035579_0_0_1 |
InParanoidi | Q91YE9 |
TreeFami | TF329831 |
Enzyme and pathway databases
Reactomei | R-MMU-74259, Purine catabolism |
SABIO-RKi | Q91YE9 |
Miscellaneous databases
BioGRID-ORCSi | 70881, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nt5c1b, mouse |
PROi | PR:Q91YE9 |
RNActi | Q91YE9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020622, Expressed in spermatid and 44 other tissues |
ExpressionAtlasi | Q91YE9, baseline and differential |
Genevisiblei | Q91YE9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR010394, 5-nucleotidase |
PANTHERi | PTHR31367, PTHR31367, 1 hit |
Pfami | View protein in Pfam PF06189, 5-nucleotidase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5NT1B_MOUSE | |
Accessioni | Q91YE9Primary (citable) accession number: Q91YE9 Secondary accession number(s): E9QNH2, Q91Y48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | June 28, 2011 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families