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Protein

Synaptopodin-2

Gene

Synpo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has an actin-binding and actin-bundling activity (PubMed:11673475). Can induce the formation of F-actin networks. At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines. Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension; may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes. Involved in regulation of cell migration. May be a tumor suppressor (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptopodin-2
Alternative name(s):
Myopodin
Gene namesi
Name:Synpo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2153070 Synpo2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi555S → A: Impairs interaction with YWHAB. 1 Publication1
Mutagenesisi602T → A: Impairs interaction with YWHAB. 1 Publication1

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001876741 – 1087Synaptopodin-2Add BLAST1087

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphoserineCombined sources1
Modified residuei319PhosphoserineBy similarity1
Modified residuei320PhosphoserineBy similarity1
Modified residuei323PhosphothreonineBy similarity1
Modified residuei540PhosphoserineBy similarity1
Modified residuei541PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei555Phosphoserine; by PKA1 Publication1
Modified residuei596PhosphoserineCombined sources1
Modified residuei602Phosphothreonine; by PKA and CaMK21 Publication1
Modified residuei603PhosphoserineCombined sources1
Modified residuei614PhosphotyrosineBy similarity1
Modified residuei618PhosphoserineCombined sources1
Modified residuei697PhosphoserineBy similarity1
Modified residuei719PhosphoserineBy similarity1
Modified residuei744PhosphothreonineBy similarity1
Modified residuei767PhosphoserineCombined sources1
Modified residuei771PhosphoserineCombined sources1
Modified residuei895PhosphoserineCombined sources1
Modified residuei899PhosphoserineCombined sources1
Modified residuei903PhosphoserineBy similarity1
Modified residuei1008PhosphoserineBy similarity1
Modified residuei1050PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PKA, and by CaMK2 at multiple sites. Dephosphorylated by calcineurin at Ser-555 and Thr-602; abrogating interaction with YWHAB and impairing nuclear import.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91YE8
PRIDEiQ91YE8

PTM databases

iPTMnetiQ91YE8
PhosphoSitePlusiQ91YE8

Expressioni

Tissue specificityi

Expressed in skeletal muscle, heart, colon, stomach, uterus and lung. Expression is restricted to muscle cell layers in colon, uterus and stomach.1 Publication

Gene expression databases

BgeeiENSMUSG00000050315 Expressed in 201 organ(s), highest expression level in gastrocnemius
CleanExiMM_SYNPO2
ExpressionAtlasiQ91YE8 baseline and differential
GenevisibleiQ91YE8 MM

Interactioni

Subunit structurei

May self-associate in muscle cells under oxidative stress (By similarity). Binds F-actin (PubMed:11673475). Interacts with ACTN2; ACTN2 is proposed to anchor SYOP2 at Z lines in mature myocytes. Interacts with AKAP6, PPP3CA and CAMK2A. Interacts (phosphorylated form) with YWHAB; YWHAB competes with ACTN2 for interaction with SYNPO2 (PubMed:15883195, PubMed:17923693). Interacts with KPNA2; mediating nuclear import of SYNOP2; dependent on interaction with YWHAB (PubMed:15883195). Interacts with IPO13; may be implicated in SYNOP2 nuclear import (By similarity). Interacts with ZYX, FLNC, ILK. Interacts with BAG3 (via WW 1 domain). May associate with the CASA complex consisting of HSPA8, HSPB8 and BAG3. Interacts with VPS18 (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91YE8, 3 interactors
MINTiQ91YE8
STRINGi10090.ENSMUSP00000102034

Structurei

3D structure databases

ProteinModelPortaliQ91YE8
SMRiQ91YE8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 88PDZPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni551 – 557Interaction with YWHAB1 Publication7
Regioni599 – 804Interaction with YWHAB1 PublicationAdd BLAST206
Regioni656 – 917Interaction with ACTN2By similarityAdd BLAST262
Regioni656 – 909F-actin bundling activityBy similarityAdd BLAST254
Regioni656 – 796F-actin bindingBy similarityAdd BLAST141
Regioni740 – 893Actin binding1 PublicationAdd BLAST154
Regioni803 – 1087Interaction with FLNCBy similarityAdd BLAST285
Regioni894 – 1087Interaction with ACTN2By similarityAdd BLAST194
Regioni993 – 1012Interaction with ZYXBy similarityAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi388 – 396Nuclear localization signal1 Publication9
Motifi611 – 614PPPY motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi390 – 395Poly-Arg6
Compositional biasi406 – 415Poly-Glu10
Compositional biasi591 – 650Pro-richAdd BLAST60
Compositional biasi745 – 859Pro-richAdd BLAST115

Domaini

The PPPY motif interacts with the WW domain 1 of BAG3.By similarity

Sequence similaritiesi

Belongs to the synaptopodin family.Curated

Phylogenomic databases

eggNOGiENOG410IIQE Eukaryota
ENOG410ZJ02 LUCA
GeneTreeiENSGT00530000063754
HOGENOMiHOG000261625
HOVERGENiHBG079226
InParanoidiQ91YE8
PhylomeDBiQ91YE8

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91YE8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGTGDFICIS MTGGAPWGFR LQGGKEEQQP LQVAKIRSQS KASGSGLREG
60 70 80 90 100
DEVVSINGNP CADLTYPEVI KLMEGITDSL HLLVKRPSSG TSETLDSESE
110 120 130 140 150
TTNHQHLTHE GPMESTTLQI QQATETQSED FFLAPVQTKV PLTEDQSNAW
160 170 180 190 200
GYAECPKEEQ APPMLGSQEG HLVEEVILRQ KAEAGQPGHV VELQLSLSKE
210 220 230 240 250
RHQCTSGPIV TLQGNDKSTS PDPDWSSQLE RTVHINSIPA PEKADTSLTS
260 270 280 290 300
STSSGRELRV IQGRDPGGAG LPQVEVILDC SDRLKAEECR LQTGRGCVAS
310 320 330 340 350
PVEGGRSEAP PSLVSFAVSS EGTEHGEDQR SGKDQSRPHK HRARHARLRR
360 370 380 390 400
SESLSEKQVK EAKSKCKSIA LLLTDAPNPN SKGVLMFKKR RRRARKYTLV
410 420 430 440 450
SYGTGELERE EEEEEDQEAG DKDEISEVAF LGTSESEVDE ELLSDVDDNT
460 470 480 490 500
QVVNFDWDSG LVDIEKRLNR GDKMEMLPDT TGKGALMFAK RRERMEQFTA
510 520 530 540 550
QNEEEKTGGM AGGGPDALQT DGLRTMTSYQ RKEESVRMQS SVSESSFQMG
560 570 580 590 600
RSLASVPQQN GFSGVSETAG AQRMFPMNRT AKPFLGSMNQ PAAPFSPTRS
610 620 630 640 650
VTSPISDFPA PPPYSAVSPP PEAFSRGVSS PVAGPAQPPP WPQPAPWSQP
660 670 680 690 700
AFYDSSEQIA SRDERIAVPA KRTGILQEAK RRGTTKPMFT FKETKVSPNP
710 720 730 740 750
ELLSLLQNAE GKRGTGGDSG PEEDYLSLGA EACNFMQSSA KQKTPPPVAP
760 770 780 790 800
KPAVKSPSSS QPVAPVSPVW SPGVAPAQRP AFSTSNPPNP PQVTAVSSIK
810 820 830 840 850
IAQPAAPPAR PASALNLAGP FKGPQAVVVS HNYTPKPSAP TPLVNAAPAG
860 870 880 890 900
AGGPSNELPG MSGKGAQLFA KRQSRMEKYV VDSDTVQAHT VRAQSPTPSL
910 920 930 940 950
PASWKYSSNV RAPPPVAYNP IHSPSYPLAA IKSQPPGAQA SKTSKKKGKK
960 970 980 990 1000
PLNTLDVMKH QPYQLNASLF TFQPPDSKDG LPQKSTVKVS SAPAMKQALP
1010 1020 1030 1040 1050
PRQANVGSPT NAQASSVYSV PAYTSQPNFF AAEATSPVSA SPVPVSVPTS
1060 1070 1080
PKQESTSTSY FVAPRPKFSA KKSGVTVQVW KPSVVEE
Note: No experimental confirmation available.
Length:1,087
Mass (Da):116,527
Last modified:August 31, 2004 - v2
Checksum:iBA2BAE2797947131
GO
Isoform 2 (identifier: Q91YE8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-330: Missing.
     331-347: SGKDQSRPHKHRARHAR → MIPCSHQFSTICVLSPG

Note: No experimental confirmation available.
Show »
Length:757
Mass (Da):80,908
Checksum:i8C1FAA539DF14B37
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q1U2E9Q1U2_MOUSE
Synaptopodin-2
Synpo2
1,257Annotation score:
D3YVV9D3YVV9_MOUSE
Synaptopodin-2
Synpo2
1,198Annotation score:
F6WWS1F6WWS1_MOUSE
Synaptopodin-2
Synpo2
216Annotation score:
A0A0G2JDM5A0A0G2JDM5_MOUSE
Synaptopodin-2
Synpo2
185Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti894Q → E in BAC37575 (PubMed:16141072).Curated1
Sequence conflicti1067K → E in BAC37575 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0114931 – 330Missing in isoform 2. CuratedAdd BLAST330
Alternative sequenceiVSP_011494331 – 347SGKDQ…ARHAR → MIPCSHQFSTICVLSPG in isoform 2. CuratedAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306625 mRNA Translation: CAC67798.1
AC121315 Genomic DNA No translation available.
AC121540 Genomic DNA No translation available.
AK079194 mRNA Translation: BAC37575.1
UniGeneiMm.34359
Mm.474733

Genome annotation databases

EnsembliENSMUST00000051443; ENSMUSP00000051570; ENSMUSG00000050315 [Q91YE8-2]
ENSMUST00000198584; ENSMUSP00000142508; ENSMUSG00000050315 [Q91YE8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ306625 mRNA Translation: CAC67798.1
AC121315 Genomic DNA No translation available.
AC121540 Genomic DNA No translation available.
AK079194 mRNA Translation: BAC37575.1
UniGeneiMm.34359
Mm.474733

3D structure databases

ProteinModelPortaliQ91YE8
SMRiQ91YE8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91YE8, 3 interactors
MINTiQ91YE8
STRINGi10090.ENSMUSP00000102034

PTM databases

iPTMnetiQ91YE8
PhosphoSitePlusiQ91YE8

Proteomic databases

PaxDbiQ91YE8
PRIDEiQ91YE8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051443; ENSMUSP00000051570; ENSMUSG00000050315 [Q91YE8-2]
ENSMUST00000198584; ENSMUSP00000142508; ENSMUSG00000050315 [Q91YE8-1]

Organism-specific databases

MGIiMGI:2153070 Synpo2

Phylogenomic databases

eggNOGiENOG410IIQE Eukaryota
ENOG410ZJ02 LUCA
GeneTreeiENSGT00530000063754
HOGENOMiHOG000261625
HOVERGENiHBG079226
InParanoidiQ91YE8
PhylomeDBiQ91YE8

Miscellaneous databases

ChiTaRSiSynpo2 mouse
PROiPR:Q91YE8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050315 Expressed in 201 organ(s), highest expression level in gastrocnemius
CleanExiMM_SYNPO2
ExpressionAtlasiQ91YE8 baseline and differential
GenevisibleiQ91YE8 MM

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYNP2_MOUSE
AccessioniPrimary (citable) accession number: Q91YE8
Secondary accession number(s): Q8C592
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: September 12, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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