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Protein

RNA-binding protein 5

Gene

Rbm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes production of a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes production of a catalytically active form of CASP2/Caspase-2 that induces apoptosis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri181 – 210RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri647 – 672C2H2-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processApoptosis, mRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 5
Alternative name(s):
Putative tumor suppressor LUCA15
RNA-binding motif protein 5
Gene namesi
Name:Rbm5
Synonyms:Luca15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933204 Rbm5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002530501 – 815RNA-binding protein 5Add BLAST815

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei59PhosphoserineBy similarity1
Modified residuei69PhosphoserineBy similarity1
Modified residuei72PhosphoserineBy similarity1
Modified residuei78PhosphoserineCombined sources1
Modified residuei444PhosphoserineBy similarity1
Modified residuei621PhosphoserineCombined sources1
Modified residuei624PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91YE7
PeptideAtlasiQ91YE7
PRIDEiQ91YE7

PTM databases

iPTMnetiQ91YE7
PhosphoSitePlusiQ91YE7

Expressioni

Gene expression databases

BgeeiENSMUSG00000032580 Expressed in 278 organ(s), highest expression level in rostral migratory stream
CleanExiMM_RBM5
ExpressionAtlasiQ91YE7 baseline and differential
GenevisibleiQ91YE7 MM

Interactioni

Subunit structurei

Component of the spliceosome A complex (also known as the prespliceosome). Appears to dissociate from the spliceosome upon formation of the spliceosome B complex (also known as the precatalytic spliceosome), in which the heterotrimeric U4/U6.U5 snRNPs are bound. Interacts with U2AF2; this interaction is direct. Also interacts with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200; these interactions may be indirect (By similarity).By similarity

Protein-protein interaction databases

BioGridi219933, 3 interactors
IntActiQ91YE7, 1 interactor
MINTiQ91YE7
STRINGi10090.ENSMUSP00000035199

Structurei

3D structure databases

ProteinModelPortaliQ91YE7
SMRiQ91YE7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YE7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 178RRM 1PROSITE-ProRule annotationAdd BLAST81
Domaini231 – 315RRM 2PROSITE-ProRule annotationAdd BLAST85
Domaini743 – 789G-patchPROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni321 – 809Required for interaction with U2AF2By similarityAdd BLAST489
Regioni452 – 535Sufficient for interaction with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200By similarityAdd BLAST84

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 71Arg-richAdd BLAST66
Compositional biasi462 – 497Tyr-richAdd BLAST36

Sequence similaritiesi

Belongs to the RBM5/RBM10 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri181 – 210RanBP2-typePROSITE-ProRule annotationAdd BLAST30
Zinc fingeri647 – 672C2H2-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0154 Eukaryota
ENOG410YNFQ LUCA
GeneTreeiENSGT00510000046476
HOGENOMiHOG000046285
HOVERGENiHBG000318
InParanoidiQ91YE7
KOiK13094
OMAiTADSYKD
OrthoDBiEOG091G01UT
PhylomeDBiQ91YE7
TreeFamiTF315789

Family and domain databases

CDDicd12752 RRM1_RBM5, 1 hit
cd12755 RRM2_RBM5, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR000467 G_patch_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR033094 RBM5
IPR034991 RBM5_RRM1
IPR034993 RBM5_RRM2
IPR000504 RRM_dom
IPR013087 Znf_C2H2_type
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PANTHERiPTHR13948:SF21 PTHR13948:SF21, 1 hit
PfamiView protein in Pfam
PF01585 G-patch, 1 hit
PF00076 RRM_1, 1 hit
PF00641 zf-RanBP, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00360 RRM, 2 hits
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54928 SSF54928, 2 hits
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS50102 RRM, 2 hits
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q91YE7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSDKRVSRT ERSGRYGSII DRDDRDERES RSRRRDSDYK RSSDDRRGDR
60 70 80 90 100
YDDYRDYDSP ERERERRNSD RSEDGYHSDG DYGEHDYRHD ISDERESKTI
110 120 130 140 150
MLRGLPITIT ESDIREMMES FEGPQPADVR LMKRKTGVSR GFAFVEFYHL
160 170 180 190 200
QDATSWMEAN QKKLVIQGKH IAMHYSNPRP KFEDWLCNKC CLNNFRKRLK
210 220 230 240 250
CFRCGADKFD SEQEVPPGTT ESAQSVDYYC DTIILRNIAP HTVVDSIMTA
260 270 280 290 300
LSPYASLAVN NIRLIKDKQT QQNRGFAFVQ LSSAMDASQL LQILQSLHPP
310 320 330 340 350
LKIDGKTIGV DFAKSARKDL VLPDGNRVSA FSVASTAIAA AQWSSTQSQS
360 370 380 390 400
GEGGSVDYSY MQPGQDGYTQ YTQYSQDYQQ FYQQQAGGLE SDTSATSGTT
410 420 430 440 450
VTTTSAAVVS QSPQLYNQTS NPPGSPTEEA QPSTSTSTQA PAASPTGVVP
460 470 480 490 500
GTKYAVPDTS TYQYDESSGY YYDPTTGLYY DPNSQYYYNS LTQQYLYWDG
510 520 530 540 550
EKETYVPAAE ASSNQQTGLP STKEGKEKKE KPKSKTAQQI AKDMERWAKS
560 570 580 590 600
LNKQKENFKN SFQPVNSLRE EERRESAAAD AGFALFEKKG ALAERQQLLP
610 620 630 640 650
ELVRNGDEEN PLKRGLVAAY SGDSDNEEEL VERLESEEEK LADWKKMACL
660 670 680 690 700
LCRRQFPNRD ALVRHQQLSD LHKQNMDIYR RSRLSEQELE ALELREREMK
710 720 730 740 750
YRDRAAERRE KYGIPEPPEP KRKKQFDAGT VNYEQPTKDG IDHSNIGNKM
760 770 780 790 800
LQAMGWREGS GLGRKCQGIT APIEAQVRLK GAGLGAKGSA YGLSGADSYK
810
DAVRKAMFAR FTEME
Length:815
Mass (Da):92,311
Last modified:December 1, 2001 - v1
Checksum:iBCDADD4A8FB4EAC9
GO
Isoform 2 (identifier: Q91YE7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-455: Missing.

Show »
Length:814
Mass (Da):92,240
Checksum:i86BD2751A6E3E1B3
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R273S4R273_MOUSE
RNA-binding protein 5
Rbm5
154Annotation score:
S4R1U7S4R1U7_MOUSE
RNA-binding protein 5
Rbm5
251Annotation score:
S4R1V5S4R1V5_MOUSE
RNA-binding protein 5
Rbm5
137Annotation score:
A0A0A6YX44A0A0A6YX44_MOUSE
RNA-binding protein 5
Rbm5
175Annotation score:
S4R184S4R184_MOUSE
RNA-binding protein 5
Rbm5
150Annotation score:
S4R1N4S4R1N4_MOUSE
RNA-binding protein 5
Rbm5
115Annotation score:
S4R2T4S4R2T4_MOUSE
RNA-binding protein 5
Rbm5
115Annotation score:
A0A0A6YY86A0A0A6YY86_MOUSE
RNA-binding protein 5
Rbm5
101Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272Q → K in BAE22042 (PubMed:16141072).Curated1
Sequence conflicti693E → G in BAE22042 (PubMed:16141072).Curated1
Sequence conflicti726F → L in BAE22042 (PubMed:16141072).Curated1
Sequence conflicti798S → P in BAE22042 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021003455Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309168 mRNA Translation: CAC69136.1
AK134183 mRNA Translation: BAE22042.1
AK146880 mRNA Translation: BAE27501.1
BC003988 mRNA Translation: AAH03988.1
BC023854 mRNA Translation: AAH23854.1
BC031899 mRNA Translation: AAH31899.1
BC043058 mRNA Translation: AAH43058.1
BC054729 mRNA Translation: AAH54729.1
CCDSiCCDS23506.1 [Q91YE7-1]
RefSeqiNP_683732.1, NM_148930.3 [Q91YE7-1]
XP_006511933.1, XM_006511870.3
XP_006511934.1, XM_006511871.3
XP_006511935.1, XM_006511872.2 [Q91YE7-1]
XP_011241223.1, XM_011242921.2
UniGeneiMm.259197

Genome annotation databases

EnsembliENSMUST00000035199; ENSMUSP00000035199; ENSMUSG00000032580 [Q91YE7-2]
ENSMUST00000182659; ENSMUSP00000138379; ENSMUSG00000032580 [Q91YE7-1]
GeneIDi83486
KEGGimmu:83486
UCSCiuc009rmv.2 mouse [Q91YE7-2]
uc009rmw.2 mouse [Q91YE7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309168 mRNA Translation: CAC69136.1
AK134183 mRNA Translation: BAE22042.1
AK146880 mRNA Translation: BAE27501.1
BC003988 mRNA Translation: AAH03988.1
BC023854 mRNA Translation: AAH23854.1
BC031899 mRNA Translation: AAH31899.1
BC043058 mRNA Translation: AAH43058.1
BC054729 mRNA Translation: AAH54729.1
CCDSiCCDS23506.1 [Q91YE7-1]
RefSeqiNP_683732.1, NM_148930.3 [Q91YE7-1]
XP_006511933.1, XM_006511870.3
XP_006511934.1, XM_006511871.3
XP_006511935.1, XM_006511872.2 [Q91YE7-1]
XP_011241223.1, XM_011242921.2
UniGeneiMm.259197

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CLVX-ray1.90C/M484-491[»]
2OL3X-ray2.90P484-491[»]
ProteinModelPortaliQ91YE7
SMRiQ91YE7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219933, 3 interactors
IntActiQ91YE7, 1 interactor
MINTiQ91YE7
STRINGi10090.ENSMUSP00000035199

PTM databases

iPTMnetiQ91YE7
PhosphoSitePlusiQ91YE7

Proteomic databases

PaxDbiQ91YE7
PeptideAtlasiQ91YE7
PRIDEiQ91YE7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035199; ENSMUSP00000035199; ENSMUSG00000032580 [Q91YE7-2]
ENSMUST00000182659; ENSMUSP00000138379; ENSMUSG00000032580 [Q91YE7-1]
GeneIDi83486
KEGGimmu:83486
UCSCiuc009rmv.2 mouse [Q91YE7-2]
uc009rmw.2 mouse [Q91YE7-1]

Organism-specific databases

CTDi10181
MGIiMGI:1933204 Rbm5

Phylogenomic databases

eggNOGiKOG0154 Eukaryota
ENOG410YNFQ LUCA
GeneTreeiENSGT00510000046476
HOGENOMiHOG000046285
HOVERGENiHBG000318
InParanoidiQ91YE7
KOiK13094
OMAiTADSYKD
OrthoDBiEOG091G01UT
PhylomeDBiQ91YE7
TreeFamiTF315789

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiRbm5 mouse
EvolutionaryTraceiQ91YE7
PROiPR:Q91YE7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032580 Expressed in 278 organ(s), highest expression level in rostral migratory stream
CleanExiMM_RBM5
ExpressionAtlasiQ91YE7 baseline and differential
GenevisibleiQ91YE7 MM

Family and domain databases

CDDicd12752 RRM1_RBM5, 1 hit
cd12755 RRM2_RBM5, 1 hit
Gene3Di3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR000467 G_patch_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR033094 RBM5
IPR034991 RBM5_RRM1
IPR034993 RBM5_RRM2
IPR000504 RRM_dom
IPR013087 Znf_C2H2_type
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PANTHERiPTHR13948:SF21 PTHR13948:SF21, 1 hit
PfamiView protein in Pfam
PF01585 G-patch, 1 hit
PF00076 RRM_1, 1 hit
PF00641 zf-RanBP, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00360 RRM, 2 hits
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54928 SSF54928, 2 hits
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS50102 RRM, 2 hits
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRBM5_MOUSE
AccessioniPrimary (citable) accession number: Q91YE7
Secondary accession number(s): Q3UZ19, Q99KV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: December 1, 2001
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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