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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (By similarity). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (PubMed:12871950). Plays a role in dendrite spine morphogenesis (PubMed:25851601).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-203641 NOSTRIN mediated eNOS trafficking
R-MMU-373753 Nephrin family interactions
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-418885 DCC mediated attractive signaling
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1920428 Wasl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253Y → E: No effect on phosphorylation. Protein preferentially localized in cytoplasm. 1 Publication1
Mutagenesisi253Y → F: Abolishes phosphorylation. Protein preferentially localized in nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001890012 – 501Neural Wiskott-Aldrich syndrome proteinAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei239Phosphoserine; by TNK2By similarity1
Modified residuei253Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication1
Modified residuei304Omega-N-methylarginineBy similarity1
Modified residuei480PhosphoserineCombined sources1
Modified residuei481PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-239, Tyr-253, Ser-480 and Ser-481 enhances actin polymerization activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ91YD9
PaxDbiQ91YD9
PRIDEiQ91YD9

PTM databases

iPTMnetiQ91YD9
PhosphoSitePlusiQ91YD9

Expressioni

Gene expression databases

BgeeiENSMUSG00000029684 Expressed in 276 organ(s), highest expression level in pituitary gland
CleanExiMM_WASL
ExpressionAtlasiQ91YD9 baseline and differential
GenevisibleiQ91YD9 MM

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42 (By similarity). Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP (PubMed:14506234). Interacts with SNX9 (By similarity). Interacts with the WW domains of PRPF40A/FBP11 (PubMed:14697212). Interacts with PTK2/FAK1 (PubMed:14676198). Interacts with PACSIN1, PACSIN2 and PACSIN3 (PubMed:11082044). Interacts with NOSTRIN. Binds to TNK2. Interacts with SNX33. Interacts with NONO (via second RRM domain); the interaction is direct. Component of a multiprotein complex with NONO and SFPQ; associates with the complex via direct interaction with NONO (By similarity).By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215819, 4 interactors
CORUMiQ91YD9
DIPiDIP-29788N
IntActiQ91YD9, 11 interactors
MINTiQ91YD9
STRINGi10090.ENSMUSP00000031695

Structurei

3D structure databases

ProteinModelPortaliQ91YD9
SMRiQ91YD9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YD9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 138WH1PROSITE-ProRule annotationAdd BLAST108
Domaini200 – 213CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini401 – 418WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini429 – 446WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 385Pro-richAdd BLAST112
Compositional biasi482 – 501Asp-richAdd BLAST20

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111H4S LUCA
GeneTreeiENSGT00730000110895
HOVERGENiHBG000222
InParanoidiQ91YD9
KOiK05747
OMAiKCQTLNT
OrthoDBiEOG091G0VCD
PhylomeDBiQ91YD9
TreeFamiTF316736

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR030214 N-WASP
IPR011993 PH-like_dom_sf
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF39 PTHR23202:SF39, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 2 hits
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 2 hits
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q91YD9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWAKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYGSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP PSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPAPSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFEDDDEWE

D
Length:501
Mass (Da):54,274
Last modified:December 1, 2001 - v1
Checksum:iF5ABF44DF9A9F716
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9CXQ9Q9CXQ9_MOUSE
Neural Wiskott-Aldrich syndrome pro...
Wasl
193Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA Translation: CAC69994.1
CCDSiCCDS19945.1
RefSeqiNP_082735.2, NM_028459.2
UniGeneiMm.1574

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684
GeneIDi73178
KEGGimmu:73178
UCSCiuc009bbv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA Translation: CAC69994.1
CCDSiCCDS19945.1
RefSeqiNP_082735.2, NM_028459.2
UniGeneiMm.1574

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3NX-ray7.00W397-474[»]
ProteinModelPortaliQ91YD9
SMRiQ91YD9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215819, 4 interactors
CORUMiQ91YD9
DIPiDIP-29788N
IntActiQ91YD9, 11 interactors
MINTiQ91YD9
STRINGi10090.ENSMUSP00000031695

PTM databases

iPTMnetiQ91YD9
PhosphoSitePlusiQ91YD9

Proteomic databases

MaxQBiQ91YD9
PaxDbiQ91YD9
PRIDEiQ91YD9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684
GeneIDi73178
KEGGimmu:73178
UCSCiuc009bbv.2 mouse

Organism-specific databases

CTDi8976
MGIiMGI:1920428 Wasl

Phylogenomic databases

eggNOGiKOG3671 Eukaryota
ENOG4111H4S LUCA
GeneTreeiENSGT00730000110895
HOVERGENiHBG000222
InParanoidiQ91YD9
KOiK05747
OMAiKCQTLNT
OrthoDBiEOG091G0VCD
PhylomeDBiQ91YD9
TreeFamiTF316736

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-203641 NOSTRIN mediated eNOS trafficking
R-MMU-373753 Nephrin family interactions
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-418885 DCC mediated attractive signaling
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiWasl mouse
EvolutionaryTraceiQ91YD9
PROiPR:Q91YD9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029684 Expressed in 276 organ(s), highest expression level in pituitary gland
CleanExiMM_WASL
ExpressionAtlasiQ91YD9 baseline and differential
GenevisibleiQ91YD9 MM

Family and domain databases

CDDicd00132 CRIB, 1 hit
cd01205 EVH1_WASP-like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.90.810.10, 2 hits
InterProiView protein in InterPro
IPR000095 CRIB_dom
IPR036936 CRIB_dom_sf
IPR030214 N-WASP
IPR011993 PH-like_dom_sf
IPR011026 WASP_C
IPR033927 WASPfam_EVH1
IPR000697 WH1/EVH1_dom
IPR003124 WH2_dom
PANTHERiPTHR23202:SF39 PTHR23202:SF39, 1 hit
PfamiView protein in Pfam
PF00786 PBD, 1 hit
PF00568 WH1, 1 hit
PF02205 WH2, 2 hits
SMARTiView protein in SMART
SM00285 PBD, 1 hit
SM00461 WH1, 1 hit
SM00246 WH2, 2 hits
SUPFAMiSSF47912 SSF47912, 2 hits
PROSITEiView protein in PROSITE
PS50108 CRIB, 1 hit
PS50229 WH1, 1 hit
PS51082 WH2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiWASL_MOUSE
AccessioniPrimary (citable) accession number: Q91YD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: October 10, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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