UniProtKB - Q91YD3 (DCP1A_MOUSE)
mRNA-decapping enzyme 1A
Dcp1a
Functioni
Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity).
Essential for embryonic development (PubMed:11836524).
By similarity1 PublicationCaution
Catalytic activityi
- a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + 2 H+ + N7-methyl-GDPBy similarityEC:3.6.1.62By similarityThis reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- enzyme activator activity Source: InterPro
- hydrolase activity Source: UniProtKB-KW
- identical protein binding Source: MGI
- kinesin binding Source: MGI
- mRNA binding Source: GO_Central
GO - Biological processi
- deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
- deadenylation-independent decapping of nuclear-transcribed mRNA Source: GO_Central
- methylguanosine-cap decapping Source: MGI
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
- positive regulation of catalytic activity Source: InterPro
- positive regulation of transcription, DNA-templated Source: MGI
- protein localization to cytoplasmic stress granule Source: MGI
- transforming growth factor beta receptor signaling pathway Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Nonsense-mediated mRNA decay |
Enzyme and pathway databases
Reactomei | R-MMU-430039, mRNA decay by 5' to 3' exoribonuclease R-MMU-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-MMU-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: mRNA-decapping enzyme 1A (EC:3.6.1.62By similarity)Alternative name(s): MAD homolog 4-interacting transcription coactivator 1 Smad4-interacting transcriptional co-activator Transcription factor SMIF |
Gene namesi | Name:Dcp1a Synonyms:Mitc1, Smif |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923151, Dcp1a |
VEuPathDBi | HostDB:ENSMUSG00000021962 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- P-body 2 Publications
Note: Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (By similarity). Colocalizes with NANOS3 in the processing bodies (PubMed:19861488).By similarity1 Publication
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: MGI
- cytoplasmic ribonucleoprotein granule Source: MGI
- P-body Source: UniProtKB
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189633 | 1 – 602 | mRNA-decapping enzyme 1AAdd BLAST | 602 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | PhosphoserineCombined sources | 1 | |
Modified residuei | 162 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 200 | PhosphoserineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 339 | PhosphoserineCombined sources | 1 | |
Modified residuei | 367 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 372 | PhosphoserineCombined sources | 1 | |
Modified residuei | 395 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 420 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 441 | PhosphoserineBy similarity | 1 | |
Modified residuei | 542 | PhosphoserineCombined sources | 1 | |
Modified residuei | 543 | PhosphoserineCombined sources | 1 | |
Modified residuei | 545 | PhosphoserineCombined sources | 1 | |
Modified residuei | 548 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 551 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q91YD3 |
jPOSTi | Q91YD3 |
MaxQBi | Q91YD3 |
PaxDbi | Q91YD3 |
PeptideAtlasi | Q91YD3 |
PRIDEi | Q91YD3 |
ProteomicsDBi | 279315 |
PTM databases
iPTMneti | Q91YD3 |
PhosphoSitePlusi | Q91YD3 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000021962, Expressed in secondary oocyte and 301 other tissues |
ExpressionAtlasi | Q91YD3, baseline and differential |
Genevisiblei | Q91YD3, MM |
Interactioni
Subunit structurei
Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3.
Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1.
Interacts with DCP1B.
Interacts with DCP2.
Interacts with DDX17 in an RNA-independent manner.
Interacts with PNRC2.
Interacts with SMAD4.
Interacts with UPF1.
Interacts with ZC3HAV1.
Interacts with ZFP36L1.
Interacts with NBDY.
Interacts with DHX34; the interaction is RNA-independent (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- kinesin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 217832, 3 interactors |
IntActi | Q91YD3, 6 interactors |
MINTi | Q91YD3 |
STRINGi | 10090.ENSMUSP00000022535 |
Miscellaneous databases
RNActi | Q91YD3, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 152 – 174 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 191 – 234 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 267 – 291 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 157 – 171 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 192 – 232 | Polar residuesSequence analysisAdd BLAST | 41 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2868, Eukaryota |
GeneTreei | ENSGT00940000158818 |
HOGENOMi | CLU_030030_0_0_1 |
InParanoidi | Q91YD3 |
OMAi | PHSFQEP |
OrthoDBi | 1509316at2759 |
PhylomeDBi | Q91YD3 |
TreeFami | TF320504 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR010334, Dcp1 IPR031953, mRNA_decap_C IPR011993, PH-like_dom_sf |
PANTHERi | PTHR16290, PTHR16290, 1 hit |
Pfami | View protein in Pfam PF06058, DCP1, 1 hit PF16741, mRNA_decap_C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALSCSTVRP RRRGSALRSK MELLSRAEQE MSLAALKQHD PYITSIADLT
60 70 80 90 100
GQVALYTFCP KANQWEKTDI EGTLFVYRRS ASPYHGFTIV NRLNMHNLVE
110 120 130 140 150
PVNKDLEFQL HEPFLLYRNA SLSIYSIWFY DKNDCHRIAK LMADVVEEET
160 170 180 190 200
RRSQQAARDK QSPSQANGCS DQRPIDILEM LSRAKDEYER NQMGGSNISS
210 220 230 240 250
PGLQPSTQLS NLGSTETLEE TPSGSQDKSA PSGHKHLTVE ELFGTSLPKE
260 270 280 290 300
QPTAMGLESE DTDKLLGDAS QKEPSSFLPF PFEQSGGAPQ SENLGIHSAA
310 320 330 340 350
HHTVQPEVST PVLITPASIA QSGDKHPPSY TLPLSPVLSP TLPAEAPTTQ
360 370 380 390 400
VPHLPRNSTM IQAVKTTPRQ KSPLLNQPVP ELSHSSLVAS QSPFRAPVSL
410 420 430 440 450
ANPAGTALPS VDLLQKLRLT PQHDQIQAQP LGKGTMAPSF SSAAGQLATP
460 470 480 490 500
ESFIEPSSKT AAARAAVSAS LSNMVLAPTL QSMQQNQDPE VFSQPKVLPS
510 520 530 540 550
AIPIAGSPLV PATTTAVSSV LLSPSVFQQT VPRAADLERK ASSPSPLTVG
560 570 580 590 600
TAESQRKPSI ILSKSQLQDT LIHLIKNDSS FLSTLHAVYL QVLTKNKDNH
NL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YCN4 | A0A286YCN4_MOUSE | 5'-(N(7)-methylguanosine 5'-triphos... | Dcp1a | 177 | Annotation score: | ||
A0A286YDC8 | A0A286YDC8_MOUSE | 5'-(N(7)-methylguanosine 5'-triphos... | Dcp1a | 419 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 481 | Q → R in strain: C57BL/6J. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ344447 mRNA Translation: CAC69875.1 BC066173 mRNA Translation: AAH66173.1 |
CCDSi | CCDS36848.1 |
RefSeqi | NP_598522.3, NM_133761.3 |
Genome annotation databases
Ensembli | ENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962 ENSMUST00000225196; ENSMUSP00000152897; ENSMUSG00000021962 |
GeneIDi | 75901 |
KEGGi | mmu:75901 |
UCSCi | uc007sva.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ344447 mRNA Translation: CAC69875.1 BC066173 mRNA Translation: AAH66173.1 |
CCDSi | CCDS36848.1 |
RefSeqi | NP_598522.3, NM_133761.3 |
3D structure databases
SMRi | Q91YD3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 217832, 3 interactors |
IntActi | Q91YD3, 6 interactors |
MINTi | Q91YD3 |
STRINGi | 10090.ENSMUSP00000022535 |
PTM databases
iPTMneti | Q91YD3 |
PhosphoSitePlusi | Q91YD3 |
Proteomic databases
EPDi | Q91YD3 |
jPOSTi | Q91YD3 |
MaxQBi | Q91YD3 |
PaxDbi | Q91YD3 |
PeptideAtlasi | Q91YD3 |
PRIDEi | Q91YD3 |
ProteomicsDBi | 279315 |
Protocols and materials databases
Antibodypediai | 73473, 379 antibodies from 34 providers |
DNASUi | 75901 |
Genome annotation databases
Ensembli | ENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962 ENSMUST00000225196; ENSMUSP00000152897; ENSMUSG00000021962 |
GeneIDi | 75901 |
KEGGi | mmu:75901 |
UCSCi | uc007sva.1, mouse |
Organism-specific databases
CTDi | 55802 |
MGIi | MGI:1923151, Dcp1a |
VEuPathDBi | HostDB:ENSMUSG00000021962 |
Phylogenomic databases
eggNOGi | KOG2868, Eukaryota |
GeneTreei | ENSGT00940000158818 |
HOGENOMi | CLU_030030_0_0_1 |
InParanoidi | Q91YD3 |
OMAi | PHSFQEP |
OrthoDBi | 1509316at2759 |
PhylomeDBi | Q91YD3 |
TreeFami | TF320504 |
Enzyme and pathway databases
Reactomei | R-MMU-430039, mRNA decay by 5' to 3' exoribonuclease R-MMU-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-MMU-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 75901, 19 hits in 62 CRISPR screens |
ChiTaRSi | Dcp1a, mouse |
PROi | PR:Q91YD3 |
RNActi | Q91YD3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021962, Expressed in secondary oocyte and 301 other tissues |
ExpressionAtlasi | Q91YD3, baseline and differential |
Genevisiblei | Q91YD3, MM |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR010334, Dcp1 IPR031953, mRNA_decap_C IPR011993, PH-like_dom_sf |
PANTHERi | PTHR16290, PTHR16290, 1 hit |
Pfami | View protein in Pfam PF06058, DCP1, 1 hit PF16741, mRNA_decap_C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCP1A_MOUSE | |
Accessioni | Q91YD3Primary (citable) accession number: Q91YD3 Secondary accession number(s): Q6NZE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families