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Protein

Sialic acid-binding Ig-like lectin 12

Gene

Siglec12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126Sialic acidBy similarity1

GO - Molecular functioni

  • monosaccharide binding Source: MGI
  • sialic acid binding Source: MGI

GO - Biological processi

  • cell adhesion Source: MGI
  • negative regulation of inflammatory response Source: MGI
  • negative regulation of phagocytosis, engulfment Source: MGI

Keywordsi

Biological processCell adhesion
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 12
Short name:
Siglec-12
Alternative name(s):
Myeloid inhibitory siglec
Short name:
MIS
Sialic acid-binding Ig-like lectin 5
Short name:
Siglec-5
Sialic acid-binding Ig-like lectin E
Short name:
Siglec-E
Short name:
mSiglec-E
Sialic acid-binding Ig-like lectin-like 1
Short name:
Siglec-L1
Gene namesi
Name:Siglec12
Synonyms:Siglec5, Siglece, Siglecl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1932475 Siglece

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 353ExtracellularSequence analysisAdd BLAST335
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 467CytoplasmicSequence analysisAdd BLAST93

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi432Y → F: Abolishes binding to PTPN6 and PTPN11. 2 Publications1
Mutagenesisi455Y → F: Reduces binding to PTPN6. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001495419 – 467Sialic acid-binding Ig-like lectin 12Add BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 176PROSITE-ProRule annotation
Disulfide bondi45 ↔ 108PROSITE-ProRule annotation
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi170 ↔ 219PROSITE-ProRule annotation
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi216N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi244N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi278 ↔ 323PROSITE-ProRule annotation
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi294N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei432Phosphotyrosine1 Publication1
Modified residuei455Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylation of Tyr-432 is required for binding to PTPN6 and PTPN11. Phosphorylation of Tyr-455 is involved in binding to PTPN6. Tyr-432 needs to be phosphorylated prior to Tyr-455.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ91Y57
PaxDbiQ91Y57
PRIDEiQ91Y57

PTM databases

iPTMnetiQ91Y57
PhosphoSitePlusiQ91Y57
SwissPalmiQ91Y57

Expressioni

Tissue specificityi

Expressed by monocytic/myeloid lineage cells. Found at higher levels in spleen, liver and heart. Found at lower levels in kidney and lung.

Gene expression databases

BgeeiENSMUSG00000030474 Expressed in 28 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SIGLEC5
MM_SIGLECE
ExpressionAtlasiQ91Y57 baseline and differential
GenevisibleiQ91Y57 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tlr4Q9QUK63EBI-16826475,EBI-1534575

Protein-protein interaction databases

BioGridi219910, 5 interactors
ELMiQ91Y57
IntActiQ91Y57, 1 interactor
STRINGi10090.ENSMUSP00000032667

Structurei

3D structure databases

ProteinModelPortaliQ91Y57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 141Ig-like V-typeAdd BLAST121
Domaini152 – 239Ig-like C2-type 1Add BLAST88
Domaini242 – 339Ig-like C2-type 2Add BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi430 – 435ITIM motif6
Motifi453 – 458SLAM-like motif6

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJT6 Eukaryota
ENOG410YKZU LUCA
GeneTreeiENSGT00760000119139
HOVERGENiHBG036161
InParanoidiQ91Y57
OMAiKTKWNYK
OrthoDBiEOG091G0445
TreeFamiTF332441

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 3 hits
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 3 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits
PS00290 IG_MHC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q91Y57-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLLLLLLLL WGIKGVEGQN PQEVFTLNVE RKVVVQEGLC VLVPCNFSYL
60 70 80 90 100
KKRLTDWTDS DPVHGFWYRE GTDRRKDSIV ATNNPIRKAV KETRNRFFLL
110 120 130 140 150
GDPWRNDCSL NIREIRKKDA GLYFFRLERG KTKYNYMWDK MTLVVTALTN
160 170 180 190 200
TPQILLPETL EAGHPSNLTC SVPWDCGWTA PPIFSWTGTS VSFLSTNTTG
210 220 230 240 250
SSVLTITPQP QDHGTNLTCQ VTLPGTNVST RMTIRLNVSY APKNLTVTIY
260 270 280 290 300
QGADSVSTIL KNGSSLPISE GQSLRLICST DSYPPANLSW SWDNLTLCPS
310 320 330 340 350
KLSKPGLLEL FPVHLKHGGV YTCQAQHALG SQHISLSLSP QSSATLSEMM
360 370 380 390 400
MGTFVGSGVT ALLFLSVCIL LLAVRSYRRK PARPAVVAPH PDALKVSVSQ
410 420 430 440 450
NPLVESQADD SSEPLPSILE AAPSSTEEEI HYATLSFHEM KPMNLWGQQD
460
TTTEYSEIKF PQRTAWP
Length:467
Mass (Da):51,901
Last modified:July 27, 2011 - v3
Checksum:iE220C29755FE21DE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RPP9A0A0U1RPP9_MOUSE
Sialic acid-binding Ig-like lectin ...
Siglece
221Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2L → M in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti24V → G in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti102D → E in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti234I → T in AAG38598 (PubMed:11171044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317298 mRNA Translation: AAG38598.1
AF329269 mRNA Translation: AAK49917.1
AC151989 Genomic DNA No translation available.
CCDSiCCDS21178.1
RefSeqiNP_112458.2, NM_031181.2
UniGeneiMm.157702

Genome annotation databases

EnsembliENSMUST00000032667; ENSMUSP00000032667; ENSMUSG00000030474
GeneIDi83382
KEGGimmu:83382
UCSCiuc009gng.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-E

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317298 mRNA Translation: AAG38598.1
AF329269 mRNA Translation: AAK49917.1
AC151989 Genomic DNA No translation available.
CCDSiCCDS21178.1
RefSeqiNP_112458.2, NM_031181.2
UniGeneiMm.157702

3D structure databases

ProteinModelPortaliQ91Y57
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219910, 5 interactors
ELMiQ91Y57
IntActiQ91Y57, 1 interactor
STRINGi10090.ENSMUSP00000032667

PTM databases

iPTMnetiQ91Y57
PhosphoSitePlusiQ91Y57
SwissPalmiQ91Y57

Proteomic databases

MaxQBiQ91Y57
PaxDbiQ91Y57
PRIDEiQ91Y57

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032667; ENSMUSP00000032667; ENSMUSG00000030474
GeneIDi83382
KEGGimmu:83382
UCSCiuc009gng.1 mouse

Organism-specific databases

CTDi83382
MGIiMGI:1932475 Siglece

Phylogenomic databases

eggNOGiENOG410IJT6 Eukaryota
ENOG410YKZU LUCA
GeneTreeiENSGT00760000119139
HOVERGENiHBG036161
InParanoidiQ91Y57
OMAiKTKWNYK
OrthoDBiEOG091G0445
TreeFamiTF332441

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:Q91Y57
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030474 Expressed in 28 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_SIGLEC5
MM_SIGLECE
ExpressionAtlasiQ91Y57 baseline and differential
GenevisibleiQ91Y57 MM

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 3 hits
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 3 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits
PS00290 IG_MHC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIG12_MOUSE
AccessioniPrimary (citable) accession number: Q91Y57
Secondary accession number(s): E9QMD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: October 10, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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