Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysosomal-associated transmembrane protein 4B

Gene

Laptm4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal-associated transmembrane protein 4BBy similarity
Gene namesi
Name:Laptm4bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1890494 Laptm4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Cell projection, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002497231 – 227Lysosomal-associated transmembrane protein 4BAdd BLAST227

Post-translational modificationi

Undergoes proteolytic cleavage following delivery to the lysosomes.By similarity
Ubiquitinated by NEDD4.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ91XQ6
PaxDbiQ91XQ6
PRIDEiQ91XQ6

PTM databases

iPTMnetiQ91XQ6
PhosphoSitePlusiQ91XQ6

Expressioni

Gene expression databases

BgeeiENSMUSG00000022257 Expressed in 297 organ(s), highest expression level in primary oocyte
CleanExiMM_LAPTM4B
ExpressionAtlasiQ91XQ6 baseline and differential
GenevisibleiQ91XQ6 MM

Interactioni

Subunit structurei

Homooligomer; upon reaching the lysosomes. Interacts with MCOLN1. Interacts with NEDD4; may play a role in the lysosomal sorting of LAPTM4B; enhances HGS association with NEDD4; mediates inhibition of EGFR degradation. Interacts with PIP5K1C; promotes SNX5 association with LAPTM4B; kinase activity of PIP5K1C is required; interaction is regulated by phosphatidylinositol 4,5-bisphosphate generated by PIP5K1C. Interacts with HGS; promotes HGS ubiquitination. Interacts with SNX5. Interacts with SLC3A2 AND SLC7A5; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation. Interacts with LRRC32; decreases TGFB1 production in regulatory T cells. Interacts with BECN1; competes with EGFR for LAPTM4B binding; regulates EGFR activity. Interacts with EGFR; positively correlates with EGFR activation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022867

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 222Required for NEDD4 interactionBy similarityAdd BLAST18

Sequence similaritiesi

Belongs to the LAPTM4/LAPTM5 transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGWG Eukaryota
ENOG4111K1Z LUCA
GeneTreeiENSGT00390000013359
HOGENOMiHOG000007698
HOVERGENiHBG000908
InParanoidiQ91XQ6
KOiK12387
OMAiTEVLVYV
OrthoDBiEOG091G0MA5
PhylomeDBiQ91XQ6
TreeFamiTF330843

Family and domain databases

InterProiView protein in InterPro
IPR004687 LAPTM4/5
IPR018401 Lysosomal-assoc_TM_prot4B
PANTHERiPTHR12479:SF6 PTHR12479:SF6, 1 hit
PfamiView protein in Pfam
PF03821 Mtp, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q91XQ6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKMVAPWTRF YSHSCCLCCH VRTGTILLGV WYLIINAVVL LILLSALADP
60 70 80 90 100
NQYHFSGSEL GGEFEFMDDA NMCIAIAISL LMILICAMAT YGAYKQHAAW
110 120 130 140 150
IIPFFCYQIF DFALNTLVAI TVLVYPNSIQ EYIRQLPPSF PYRDDIMSVN
160 170 180 190 200
PTCLVLIILL FIGILLTLKG YLISCVWSCY RYINGRNSSD VLVYVTSNDT
210 220
TVLLPPYDDA TAVPSTAKEP PPPYVSA
Length:227
Mass (Da):25,395
Last modified:December 1, 2001 - v1
Checksum:iFD3DC53C2682818D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BPV6A0A2I3BPV6_MOUSE
Lysosomal-associated transmembrane ...
Laptm4b
159Annotation score:
A0A2I3BQL3A0A2I3BQL3_MOUSE
Lysosomal-associated transmembrane ...
Laptm4b
89Annotation score:
A0A2I3BQX5A0A2I3BQX5_MOUSE
Lysosomal-associated transmembrane ...
Laptm4b
44Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317418 mRNA Translation: AAK69596.1
AK077269 mRNA Translation: BAC36721.1
AK077933 mRNA Translation: BAC37072.1
AK079581 mRNA Translation: BAC37691.1
AK082807 mRNA Translation: BAC38630.1
BC019120 mRNA Translation: AAH19120.1
CCDSiCCDS27416.1
RefSeqiNP_277056.1, NM_033521.3
UniGeneiMm.197518

Genome annotation databases

EnsembliENSMUST00000022867; ENSMUSP00000022867; ENSMUSG00000022257
GeneIDi114128
KEGGimmu:114128
UCSCiuc007vlj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317418 mRNA Translation: AAK69596.1
AK077269 mRNA Translation: BAC36721.1
AK077933 mRNA Translation: BAC37072.1
AK079581 mRNA Translation: BAC37691.1
AK082807 mRNA Translation: BAC38630.1
BC019120 mRNA Translation: AAH19120.1
CCDSiCCDS27416.1
RefSeqiNP_277056.1, NM_033521.3
UniGeneiMm.197518

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022867

PTM databases

iPTMnetiQ91XQ6
PhosphoSitePlusiQ91XQ6

Proteomic databases

MaxQBiQ91XQ6
PaxDbiQ91XQ6
PRIDEiQ91XQ6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022867; ENSMUSP00000022867; ENSMUSG00000022257
GeneIDi114128
KEGGimmu:114128
UCSCiuc007vlj.1 mouse

Organism-specific databases

CTDi55353
MGIiMGI:1890494 Laptm4b

Phylogenomic databases

eggNOGiENOG410IGWG Eukaryota
ENOG4111K1Z LUCA
GeneTreeiENSGT00390000013359
HOGENOMiHOG000007698
HOVERGENiHBG000908
InParanoidiQ91XQ6
KOiK12387
OMAiTEVLVYV
OrthoDBiEOG091G0MA5
PhylomeDBiQ91XQ6
TreeFamiTF330843

Miscellaneous databases

ChiTaRSiLaptm4b mouse
PROiPR:Q91XQ6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022257 Expressed in 297 organ(s), highest expression level in primary oocyte
CleanExiMM_LAPTM4B
ExpressionAtlasiQ91XQ6 baseline and differential
GenevisibleiQ91XQ6 MM

Family and domain databases

InterProiView protein in InterPro
IPR004687 LAPTM4/5
IPR018401 Lysosomal-assoc_TM_prot4B
PANTHERiPTHR12479:SF6 PTHR12479:SF6, 1 hit
PfamiView protein in Pfam
PF03821 Mtp, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLAP4B_MOUSE
AccessioniPrimary (citable) accession number: Q91XQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again