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Protein

Vacuolar protein-sorting-associated protein 36

Gene

Vps36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • endosomal transport Source: MGI
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ESCRT-II complex subunit VPS36
Gene namesi
Name:Vps36
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1917410 Vps36

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152231 – 386Vacuolar protein-sorting-associated protein 36Add BLAST386

Proteomic databases

EPDiQ91XD6
MaxQBiQ91XD6
PaxDbiQ91XD6
PRIDEiQ91XD6

PTM databases

iPTMnetiQ91XD6
PhosphoSitePlusiQ91XD6

Expressioni

Gene expression databases

BgeeiENSMUSG00000031479 Expressed in 273 organ(s), highest expression level in urothelium
CleanExiMM_VPS36
ExpressionAtlasiQ91XD6 baseline and differential
GenevisibleiQ91XD6 MM

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (By similarity). Interacts with VPS25, SNF8, TSG101 and CHMP6 (By similarity). Interacts (via GLUE domain) with ubiquitin (PubMed:15755741). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (By similarity). Interacts with ECPAS (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UBCP629902EBI-8318017,EBI-413053From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213894, 2 interactors
DIPiDIP-29247N
IntActiQ91XD6, 2 interactors
MINTiQ91XD6
STRINGi10090.ENSMUSP00000033866

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ91XD6
SMRiQ91XD6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91XD6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88GLUE N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini105 – 138GLUE C-terminalPROSITE-ProRule annotationAdd BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili160 – 185Sequence analysisAdd BLAST26

Domaini

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.1 Publication

Sequence similaritiesi

Belongs to the VPS36 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2760 Eukaryota
ENOG410XR74 LUCA
GeneTreeiENSGT00390000017209
HOGENOMiHOG000006822
HOVERGENiHBG083632
InParanoidiQ91XD6
KOiK12190
OMAiRKTRIIM
OrthoDBiEOG091G09HR
PhylomeDBiQ91XD6
TreeFamiTF314770

Family and domain databases

Gene3Di1.10.10.10, 2 hits
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR021648 GLUE_dom
IPR011993 PH-like_dom_sf
IPR037855 Vps36
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13128 PTHR13128, 1 hit
PfamiView protein in Pfam
PF11605 Vps36_ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
PROSITEiView protein in PROSITE
PS51495 GLUE, 1 hit

Sequencei

Sequence statusi: Complete.

Q91XD6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDRFVWTSGL LEINETLVIQ QRGVRVYDGE EKIKFDAGTL LLSTHRLIWR
60 70 80 90 100
DQKNNECCMA IPLSQIVFIE EQAAGIGKSA KIVVHLHPAP SNKEPGPFQS
110 120 130 140 150
SKNSYIRLSF KEHGQIEFYR RLSEEMTQRR WETVPVSQSL QTNKGPQPGR
160 170 180 190 200
VRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN
210 220 230 240 250
KIKEKQGDVT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA
260 270 280 290 300
GILQAPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP
310 320 330 340 350
IRLRVFDSGV MVIELQTHKE EEMVASALET VSERGSLTSE EFAKLVGMSV
360 370 380
LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQN
Length:386
Mass (Da):43,736
Last modified:December 1, 2001 - v1
Checksum:i0036479499DD89C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010811 mRNA Translation: AAH10811.1
AK008946 mRNA Translation: BAB25984.1
CCDSiCCDS22171.1
RefSeqiNP_081614.1, NM_027338.1
UniGeneiMm.41453

Genome annotation databases

EnsembliENSMUST00000033866; ENSMUSP00000033866; ENSMUSG00000031479
GeneIDi70160
KEGGimmu:70160
UCSCiuc009lct.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010811 mRNA Translation: AAH10811.1
AK008946 mRNA Translation: BAB25984.1
CCDSiCCDS22171.1
RefSeqiNP_081614.1, NM_027338.1
UniGeneiMm.41453

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DX5X-ray3.35A1-139[»]
ProteinModelPortaliQ91XD6
SMRiQ91XD6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213894, 2 interactors
DIPiDIP-29247N
IntActiQ91XD6, 2 interactors
MINTiQ91XD6
STRINGi10090.ENSMUSP00000033866

PTM databases

iPTMnetiQ91XD6
PhosphoSitePlusiQ91XD6

Proteomic databases

EPDiQ91XD6
MaxQBiQ91XD6
PaxDbiQ91XD6
PRIDEiQ91XD6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033866; ENSMUSP00000033866; ENSMUSG00000031479
GeneIDi70160
KEGGimmu:70160
UCSCiuc009lct.1 mouse

Organism-specific databases

CTDi51028
MGIiMGI:1917410 Vps36

Phylogenomic databases

eggNOGiKOG2760 Eukaryota
ENOG410XR74 LUCA
GeneTreeiENSGT00390000017209
HOGENOMiHOG000006822
HOVERGENiHBG083632
InParanoidiQ91XD6
KOiK12190
OMAiRKTRIIM
OrthoDBiEOG091G09HR
PhylomeDBiQ91XD6
TreeFamiTF314770

Enzyme and pathway databases

ReactomeiR-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSiVps36 mouse
EvolutionaryTraceiQ91XD6
PROiPR:Q91XD6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031479 Expressed in 273 organ(s), highest expression level in urothelium
CleanExiMM_VPS36
ExpressionAtlasiQ91XD6 baseline and differential
GenevisibleiQ91XD6 MM

Family and domain databases

Gene3Di1.10.10.10, 2 hits
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR021648 GLUE_dom
IPR011993 PH-like_dom_sf
IPR037855 Vps36
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13128 PTHR13128, 1 hit
PfamiView protein in Pfam
PF11605 Vps36_ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
PROSITEiView protein in PROSITE
PS51495 GLUE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPS36_MOUSE
AccessioniPrimary (citable) accession number: Q91XD6
Secondary accession number(s): Q9CVA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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