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Protein

Acidic mammalian chitinase

Gene

Chia

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Presence in saliva and gastric juice suggests a function as a digestive enzyme.4 Publications

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication

pH dependencei

Optimum pH is below 2.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Proton donorPROSITE-ProRule annotation1
Binding sitei141ChitooligosaccharideBy similarity1
Binding sitei360ChitooligosaccharideBy similarity1

GO - Molecular functioni

  • chitinase activity Source: UniProtKB
  • chitin binding Source: GO_Central
  • kinase binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processApoptosis, Carbohydrate metabolism, Chitin degradation, Immunity, Inflammatory response, Polysaccharide degradation
LigandChitin-binding

Enzyme and pathway databases

ReactomeiR-MMU-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiCBM14 Carbohydrate-Binding Module Family 14
GH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic mammalian chitinase (EC:3.2.1.14)
Short name:
AMCase
Alternative name(s):
YNL
Gene namesi
Name:Chia
Synonyms:Chia1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1932052 Chia1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi208H → N: Strongly reduced activity at low pH, with minor effect on activity at neutral pH. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2052027
GuidetoPHARMACOLOGYi2982

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000001194522 – 473Acidic mammalian chitinaseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 51PROSITE-ProRule annotation
Disulfide bondi49 ↔ 394PROSITE-ProRule annotation
Disulfide bondi307 ↔ 372PROSITE-ProRule annotation
Disulfide bondi457 ↔ 470PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ91XA9
PaxDbiQ91XA9
PRIDEiQ91XA9

PTM databases

iPTMnetiQ91XA9
PhosphoSitePlusiQ91XA9

Expressioni

Tissue specificityi

Detected in macrophages and lung epithelial cells. Detected in the acinar cells of parotid gland and von Ebner's gland but not in submandibular and sublingual glands. Detected in gastric chief cells. Also present in parotid glandular saliva and gastric juice (at protein level). Highly expressed in submandibular gland (PubMed:11085997) and stomach. Highly expressed in parotid gland but not in submandibular and sublingual glands (PubMed:12133911). In tongue, expressed only in von Ebner's gland. Expressed at lower levels in lung.5 Publications

Developmental stagei

In parotid gland, weak expression detected at postnatal day P12, with levels increasing towards P16. In stomach, first detected at P16, with expression reaching adult levels during P20-24.1 Publication

Inductioni

Up-regulated upon pulmonary inflammation elicited by sensitization and aerosol challenge with the aeroallergen ovalbumin. Up-regulated during T-helper cell type 2 (Th2) inflammation. Induction is mediated by IL-13.1 Publication

Gene expression databases

BgeeiENSMUSG00000062778
CleanExiMM_CHIA
ExpressionAtlasiQ91XA9 baseline and differential
GenevisibleiQ91XA9 MM

Interactioni

Subunit structurei

Interacts with EGFR.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91XA9, 1 interactor
STRINGi10090.ENSMUSP00000078134

Chemistry databases

BindingDBiQ91XA9

Structurei

3D structure databases

ProteinModelPortaliQ91XA9
SMRiQ91XA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini424 – 473Chitin-binding type-2PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Chitooligosaccharide bindingBy similarity2
Regioni97 – 100Chitooligosaccharide bindingBy similarity4
Regioni210 – 213Chitooligosaccharide bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806 Eukaryota
COG3325 LUCA
GeneTreeiENSGT00550000074323
HOGENOMiHOG000111109
HOVERGENiHBG011684
InParanoidiQ91XA9
KOiK01183
OMAiTYSPNFA
OrthoDBiEOG091G014W
PhylomeDBiQ91XA9
TreeFamiTF315610

Family and domain databases

Gene3Di3.10.50.10, 1 hit
InterProiView protein in InterPro
IPR002557 Chitin-bd_dom
IPR036508 Chitin-bd_dom_sf
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF01607 CBM_14, 1 hit
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00494 ChtBD2, 1 hit
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
SSF57625 SSF57625, 1 hit
PROSITEiView protein in PROSITE
PS50940 CHIT_BIND_II, 1 hit
PS01095 CHITINASE_18, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91XA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLLLVTGL ALLLNAQLGS AYNLICYFTN WAQYRPGLGS FKPDDINPCL
60 70 80 90 100
CTHLIYAFAG MQNNEITTIE WNDVTLYKAF NDLKNRNSKL KTLLAIGGWN
110 120 130 140 150
FGTAPFTTMV STSQNRQTFI TSVIKFLRQY GFDGLDLDWE YPGSRGSPPQ
160 170 180 190 200
DKHLFTVLVK EMREAFEQEA IESNRPRLMV TAAVAGGISN IQAGYEIPEL
210 220 230 240 250
SKYLDFIHVM TYDLHGSWEG YTGENSPLYK YPTETGSNAY LNVDYVMNYW
260 270 280 290 300
KNNGAPAEKL IVGFPEYGHT FILRNPSDNG IGAPTSGDGP AGPYTRQAGF
310 320 330 340 350
WAYYEICTFL RSGATEVWDA SQEVPYAYKA NEWLGYDNIK SFSVKAQWLK
360 370 380 390 400
QNNFGGAMIW AIDLDDFTGS FCDQGKFPLT STLNKALGIS TEGCTAPDVP
410 420 430 440 450
SEPVTTPPGS GSGGGSSGGS SGGSGFCADK ADGLYPVADD RNAFWQCING
460 470
ITYQQHCQAG LVFDTSCNCC NWP
Length:473
Mass (Da):52,003
Last modified:October 24, 2003 - v2
Checksum:i333C874477476695
GO

Sequence cautioni

The sequence AAH11134 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293P → A in AAG60018 (PubMed:11085997).Curated1
Sequence conflicti405T → S in ABK78778 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290003 mRNA Translation: AAG60018.1
DQ349202 mRNA Translation: ABC86699.1
EF094027 mRNA Translation: ABK78778.1
AK008633 mRNA Translation: BAB25795.1
AK160173 mRNA Translation: BAE35672.1
CH466608 Genomic DNA Translation: EDL07527.1
BC011134 mRNA Translation: AAH11134.1 Different initiation.
BC034548 mRNA Translation: AAH34548.1
AF154571 mRNA Translation: AAF31644.1
CCDSiCCDS38585.1
RefSeqiNP_075675.2, NM_023186.3
UniGeneiMm.46418

Genome annotation databases

EnsembliENSMUST00000079132; ENSMUSP00000078134; ENSMUSG00000062778
GeneIDi81600
KEGGimmu:81600
UCSCiuc008qvv.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCHIA_MOUSE
AccessioniPrimary (citable) accession number: Q91XA9
Secondary accession number(s): A0T468
, B8K282, Q3TVE7, Q99PH2, Q9D803, Q9JLN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: July 18, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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