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Protein

Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1

Gene

Pomgnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.By similarity

Cofactori

Mn2+By similarityNote: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129CarbohydrateBy similarity1
Binding sitei179CarbohydrateBy similarity1
Binding sitei207CarbohydrateBy similarity1
Binding sitei338UDP-GlcNAcBy similarity1
Binding sitei371UDP-GlcNAcBy similarity1
Metal bindingi395ManganeseBy similarity1
Metal bindingi500ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi307 – 311UDP-GlcNAcBy similarity5
Nucleotide bindingi394 – 395UDP-GlcNAcBy similarity2
Nucleotide bindingi506 – 507UDP-GlcNAcBy similarity2

GO - Molecular functioni

GO - Biological processi

  • O-glycan processing Source: UniProtKB
  • protein O-linked glycosylation Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5173105 O-linked glycosylation
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (EC:2.4.1.-By similarity)
Short name:
POMGnT1
Gene namesi
Name:Pomgnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915523 Pomgnt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini59 – 660LumenalSequence analysisAdd BLAST602

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913911 – 660Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Disulfide bondi254 ↔ 281By similarity
Disulfide bondi269 ↔ 279By similarity
Disulfide bondi421 ↔ 490By similarity
Disulfide bondi562 ↔ 596By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ91X88
PeptideAtlasiQ91X88
PRIDEiQ91X88

PTM databases

iPTMnetiQ91X88
PhosphoSitePlusiQ91X88
SwissPalmiQ91X88

Expressioni

Tissue specificityi

Expressed at basal body and daughter centriole of photoreceptor cells (at protein level).1 Publication

Developmental stagei

Broadly expressed in late embryonic and early postnatal cerebellar neurons, including premigratory granule neurons of the external granule cell layer. Expression is maintained in neurons of the internal granule cell layer after migration is complete. Expressed in Purkinje cells throughout development. Expressed in Bergmann glial scaffolds used by granule cells during early posnatal radial migration.1 Publication

Gene expression databases

BgeeiENSMUSG00000028700 Expressed in 291 organ(s), highest expression level in saliva-secreting gland
GenevisibleiQ91X88 MM

Interactioni

Subunit structurei

Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102107

Structurei

3D structure databases

ProteinModelPortaliQ91X88
SMRiQ91X88
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 288Carbohydrate-binding stem domainBy similarityAdd BLAST197
Regioni300 – 646CatalyticBy similarityAdd BLAST347
Regioni473 – 481Interaction with O-glycosylated substrate glycoproteinBy similarity9
Regioni506 – 512Interaction with O-glycosylated substrate glycoproteinBy similarity7
Regioni600 – 605Interaction with O-glycosylated substrate glycoproteinBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi263 – 267Poly-Arg5

Domaini

The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFXX Eukaryota
ENOG410YMAS LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000231121
HOVERGENiHBG055279
InParanoidiQ91X88
KOiK09666
OMAiDESLYCI
OrthoDBiEOG091G00JT
PhylomeDBiQ91X88
TreeFamiTF320555

Family and domain databases

CDDicd13937 PANDER_GnT-1_2_like, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR039477 ILEI/PANDER_dom
IPR029044 Nucleotide-diphossugar_trans
IPR039474 POMGNT1_PANDER-like
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
PF15711 ILEI, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q91X88-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT
60 70 80 90 100
VIVNIKLILD TRRAISEANE DPEPEQDYDE ALGRLESPRR RGSSPRRVLD
110 120 130 140 150
VEVYSSRSKV YVAVDGTTVL EDEAREQGRG IHVIVLNQAT GHVMAKRVFD
160 170 180 190 200
TYSPHEDEAM VLFLNMVAPG RVLICTVKDE GSFHLKDTAK ALLRSLGSQA
210 220 230 240 250
GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL KTDVPLSSAE
260 270 280 290 300
EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL
310 320 330 340 350
NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL
360 370 380 390 400
FGLRGIQHTP ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV
410 420 430 440 450
DFFSFLSQSI HLLEEDDSLY CISAWNDQGY EHTAEDPALL YRVETMPGLG
460 470 480 490 500
WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR MPEQRRGREC IIPDVSRSYH
510 520 530 540 550
FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA YEVEIHRLLS
560 570 580 590 600
EAEVLDHSKD PCEDSFLPDT EGHTYVAFIR METDDDFATW TQLAKCLHIW
610 620 630 640 650
DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSVKKPPSVT PIFLEPPPKE
660
EGAPGAAEQT
Length:660
Mass (Da):75,110
Last modified:December 1, 2001 - v1
Checksum:i4DE09765C0F3EF7C
GO
Isoform 2 (identifier: Q91X88-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
     23-40: LTWKYKLTNQRALRRFCQ → MEGTKEVKDSNGKIQDHG

Show »
Length:638
Mass (Da):72,068
Checksum:iA8656C7EA33D2767
GO
Isoform 3 (identifier: Q91X88-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     218-250: Missing.

Show »
Length:627
Mass (Da):71,725
Checksum:i334E4F56BC5D342B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti424A → S in BAB29863 (PubMed:16141072).Curated1
Sequence conflicti557H → P in BAB22251 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0149721 – 22Missing in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_01497323 – 40LTWKY…RRFCQ → MEGTKEVKDSNGKIQDHG in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_014974218 – 250Missing in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053220 mRNA Translation: BAC55021.1
AB053221 Genomic DNA Translation: BAC55022.1
AK002638 mRNA Translation: BAB22251.1
AK019640 mRNA Translation: BAB31822.1
AK015478 mRNA Translation: BAB29863.1
AL611947 Genomic DNA Translation: CAM20673.1
BC011201 mRNA Translation: AAH11201.1
CCDSiCCDS18506.1 [Q91X88-1]
CCDS18507.1 [Q91X88-3]
CCDS71450.1 [Q91X88-2]
RefSeqiNP_001277587.1, NM_001290658.1 [Q91X88-2]
NP_080927.1, NM_026651.2 [Q91X88-1]
NP_084062.2, NM_029786.2 [Q91X88-3]
UniGeneiMm.196045

Genome annotation databases

EnsembliENSMUST00000106494; ENSMUSP00000102103; ENSMUSG00000028700 [Q91X88-2]
ENSMUST00000106496; ENSMUSP00000102105; ENSMUSG00000028700 [Q91X88-3]
ENSMUST00000106498; ENSMUSP00000102107; ENSMUSG00000028700 [Q91X88-1]
ENSMUST00000120083; ENSMUSP00000112751; ENSMUSG00000028700 [Q91X88-1]
ENSMUST00000121052; ENSMUSP00000112911; ENSMUSG00000028700 [Q91X88-1]
GeneIDi68273
KEGGimmu:68273
UCSCiuc008ugh.2 mouse [Q91X88-1]
uc008ugi.2 mouse [Q91X88-3]
uc008ugj.1 mouse [Q91X88-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

POMGnT1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053220 mRNA Translation: BAC55021.1
AB053221 Genomic DNA Translation: BAC55022.1
AK002638 mRNA Translation: BAB22251.1
AK019640 mRNA Translation: BAB31822.1
AK015478 mRNA Translation: BAB29863.1
AL611947 Genomic DNA Translation: CAM20673.1
BC011201 mRNA Translation: AAH11201.1
CCDSiCCDS18506.1 [Q91X88-1]
CCDS18507.1 [Q91X88-3]
CCDS71450.1 [Q91X88-2]
RefSeqiNP_001277587.1, NM_001290658.1 [Q91X88-2]
NP_080927.1, NM_026651.2 [Q91X88-1]
NP_084062.2, NM_029786.2 [Q91X88-3]
UniGeneiMm.196045

3D structure databases

ProteinModelPortaliQ91X88
SMRiQ91X88
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102107

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

PTM databases

iPTMnetiQ91X88
PhosphoSitePlusiQ91X88
SwissPalmiQ91X88

Proteomic databases

PaxDbiQ91X88
PeptideAtlasiQ91X88
PRIDEiQ91X88

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106494; ENSMUSP00000102103; ENSMUSG00000028700 [Q91X88-2]
ENSMUST00000106496; ENSMUSP00000102105; ENSMUSG00000028700 [Q91X88-3]
ENSMUST00000106498; ENSMUSP00000102107; ENSMUSG00000028700 [Q91X88-1]
ENSMUST00000120083; ENSMUSP00000112751; ENSMUSG00000028700 [Q91X88-1]
ENSMUST00000121052; ENSMUSP00000112911; ENSMUSG00000028700 [Q91X88-1]
GeneIDi68273
KEGGimmu:68273
UCSCiuc008ugh.2 mouse [Q91X88-1]
uc008ugi.2 mouse [Q91X88-3]
uc008ugj.1 mouse [Q91X88-2]

Organism-specific databases

CTDi55624
MGIiMGI:1915523 Pomgnt1

Phylogenomic databases

eggNOGiENOG410IFXX Eukaryota
ENOG410YMAS LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000231121
HOVERGENiHBG055279
InParanoidiQ91X88
KOiK09666
OMAiDESLYCI
OrthoDBiEOG091G00JT
PhylomeDBiQ91X88
TreeFamiTF320555

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-5173105 O-linked glycosylation

Miscellaneous databases

ChiTaRSiPomgnt1 mouse
PROiPR:Q91X88
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028700 Expressed in 291 organ(s), highest expression level in saliva-secreting gland
GenevisibleiQ91X88 MM

Family and domain databases

CDDicd13937 PANDER_GnT-1_2_like, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR039477 ILEI/PANDER_dom
IPR029044 Nucleotide-diphossugar_trans
IPR039474 POMGNT1_PANDER-like
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
PF15711 ILEI, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPMGT1_MOUSE
AccessioniPrimary (citable) accession number: Q91X88
Secondary accession number(s): A2A8F8
, Q9D2H7, Q9D5D3, Q9DCN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 1, 2001
Last modified: November 7, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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