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Protein

S-adenosylmethionine synthase isoform type-1

Gene

Mat1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.1 Publication

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (Mat2a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase isoform type-2 (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase isoform type-1 (Mat1a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24MagnesiumBy similarity1
Binding sitei30ATPBy similarity1
Metal bindingi58PotassiumBy similarity1
Binding sitei71MethionineBy similarity1
Binding sitei114MethionineBy similarity1
Binding sitei259ATPBy similarity1
Binding sitei259Methionine; shared with neighboring subunitBy similarity1
Binding sitei282ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei286ATP; shared with neighboring subunitBy similarity1
Binding sitei290ATP; shared with neighboring subunitBy similarity1
Binding sitei290MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 182ATPBy similarity3
Nucleotide bindingi248 – 251ATPBy similarity4
Nucleotide bindingi265 – 266ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-1614635 Sulfur amino acid metabolism
R-MMU-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase isoform type-1 (EC:2.5.1.61 Publication)
Short name:
AdoMet synthase 1
Alternative name(s):
Methionine adenosyltransferase 1
Short name:
MAT 1
Gene namesi
Name:Mat1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:88017 Mat1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001744331 – 396S-adenosylmethionine synthase isoform type-1Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 61By similarity
Modified residuei121S-nitrosocysteineBy similarity1

Post-translational modificationi

S-nitrosylation of Cys-121 inactivates the enzyme.By similarity
An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.By similarity

Keywords - PTMi

Disulfide bond, S-nitrosylation

Proteomic databases

MaxQBiQ91X83
PaxDbiQ91X83
PeptideAtlasiQ91X83
PRIDEiQ91X83

2D gel databases

REPRODUCTION-2DPAGEiQ91X83

PTM databases

iPTMnetiQ91X83
PhosphoSitePlusiQ91X83
SwissPalmiQ91X83

Expressioni

Gene expression databases

BgeeiENSMUSG00000037798
CleanExiMM_MAT1A
GenevisibleiQ91X83 MM

Interactioni

Subunit structurei

Homotetramer (MAT-I); dimer of dimers. Homodimer (MAT-III).By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-3182 Methionine adenosyltransferase complex variant 1
CPX-3183 Methionine adenosyltransferase complex variant 3
DIPiDIP-45874N
IntActiQ91X83, 4 interactors
MINTiQ91X83
STRINGi10090.ENSMUSP00000044288

Structurei

3D structure databases

ProteinModelPortaliQ91X83
SMRiQ91X83
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 126Flexible loopBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506 Eukaryota
COG0192 LUCA
GeneTreeiENSGT00500000044811
HOGENOMiHOG000245710
HOVERGENiHBG001562
InParanoidiQ91X83
KOiK00789
OMAiSEFPWEI
OrthoDBiEOG091G08Z2
PhylomeDBiQ91X83
TreeFamiTF300511

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q91X83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD
60 70 80 90 100
PNAKVACETV CKTGMVLLCG EITSVAMVDY QRVVRDTIKH IGYDDSAKGF
110 120 130 140 150
DFKTCNVLVA LEQQSPDIAQ CVHLDRNEED VGAGDQGLMF GYATDETEEC
160 170 180 190 200
MPLTIVLAHK LNTRIADLRR SGVLPWLRPD SKTQVTVQYM QDNGAVIPVR
210 220 230 240 250
IHTIVISVQH NEDITLEAMQ EALKEQVIKA VVPAKYLDED TVYHLQPSGR
260 270 280 290 300
FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR
310 320 330 340 350
WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSN KTERELLEVV
360 370 380 390
NKNFDLRPGV IVRDLDLKKP IYQKTACYGH FGRSEFPWEV PKKLVF
Length:396
Mass (Da):43,509
Last modified:December 1, 2001 - v1
Checksum:iAAA593A669551EB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149542 mRNA Translation: BAE28947.1
AK153832 mRNA Translation: BAE32202.1
BC011211 mRNA Translation: AAH11211.1
CCDSiCCDS26959.1
PIRiA47151
RefSeqiNP_598414.1, NM_133653.3
XP_006518513.1, XM_006518450.2
UniGeneiMm.14064
Mm.486779

Genome annotation databases

EnsembliENSMUST00000047286; ENSMUSP00000044288; ENSMUSG00000037798
ENSMUST00000225720; ENSMUSP00000153488; ENSMUSG00000037798
GeneIDi11720
KEGGimmu:11720
UCSCiuc007tcm.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMETK1_MOUSE
AccessioniPrimary (citable) accession number: Q91X83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: December 1, 2001
Last modified: June 20, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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