UniProtKB - Q91X58 (ZFN2B_MOUSE)
AN1-type zinc finger protein 2B
Zfand2b
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 52 | AN1-type 1PROSITE-ProRule annotationAdd BLAST | 46 | |
Zinc fingeri | 97 – 145 | AN1-type 2PROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- K48-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein targeting to ER Source: UniProtKB
- regulation of insulin-like growth factor receptor signaling pathway Source: UniProtKB
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: MGI
Keywordsi
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: AN1-type zinc finger protein 2BCuratedAlternative name(s): Arsenite-inducible RNA-associated protein-like protein1 Publication Short name: AIRAP-like protein1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916068, Zfand2b |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Lipid-anchor 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- anchored component of membrane Source: UniProtKB
- proteasome complex Source: Ensembl
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 163 | S → A: No effect on association with the proteasome complex; when associated with A-173 and A-187. 1 Publication | 1 | |
Mutagenesisi | 163 | S → D: No effect on association with the proteasome complex; when associated with D-173 and D-187. 1 Publication | 1 | |
Mutagenesisi | 173 | S → A: No effect on association with the proteasome complex; when associated with A-163 and A-187. 1 Publication | 1 | |
Mutagenesisi | 173 | S → D: No effect on association with the proteasome complex; when associated with D-163 and D-187. 1 Publication | 1 | |
Mutagenesisi | 187 | S → A: No effect on association with the proteasome complex; when associated with A-163 and A-173. 1 Publication | 1 | |
Mutagenesisi | 187 | S → D: No effect on association with the proteasome complex; when associated with D-163 and D-173. 1 Publication | 1 | |
Mutagenesisi | 201 | A → Q: Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-229 and Q-231. 2 Publications | 1 | |
Mutagenesisi | 202 | L → A: Decreased 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 205 | A → Q: Decreased 'Lys-48'-linked polyubiquitin binding. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-229 and Q-231. 1 Publication | 1 | |
Mutagenesisi | 206 | L → A: Decreased 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 213 – 220 | AKPQVLSS → QQQQQQQQ: No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 8 | |
Mutagenesisi | 214 | K → A or G: No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 215 | P → A or G: No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 215 | Missing : No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 218 | L → A: No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 226 | L → A: No effect on 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 227 | A → Q: Decreased 'Lys-48'-linked polyubiquitin binding; when associated with A-230. 1 Publication | 1 | |
Mutagenesisi | 228 | L → A: Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with BAG6. Decreased association with the proteasome complex. 1 Publication | 1 | |
Mutagenesisi | 229 | A → Q: Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-201 and Q-231. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-205 and Q-231. 3 Publications | 1 | |
Mutagenesisi | 230 | Q → A: Decreased 'Lys-48'-linked polyubiquitin binding; when associated with Q-227. 1 Publication | 1 | |
Mutagenesisi | 231 | A → Q: Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-201 and Q-229. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-205 and Q-229. 3 Publications | 1 | |
Mutagenesisi | 233 | S → G: Decreased 'Lys-48'-linked polyubiquitin binding. 1 Publication | 1 | |
Mutagenesisi | 254 | C → S: Loss of localization to the endoplasmic reticulum membrane. Loss of interaction with BAG6. No effect on association with the proteasome complex. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000232877 | 1 – 254 | AN1-type zinc finger protein 2BAdd BLAST | 254 | |
PropeptideiPRO_0000444336 | 255 – 257 | Removed in mature form1 Publication | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 163 | Phosphoserine; by MAPK141 Publication | 1 | |
Modified residuei | 173 | Phosphoserine; by MAPK141 Publication | 1 | |
Modified residuei | 187 | Phosphoserine; by MAPK141 Publication | 1 | |
Modified residuei | 254 | Cysteine methyl ester1 Publication | 1 | |
Lipidationi | 254 | S-geranylgeranyl cysteine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | Q91X58 |
jPOSTi | Q91X58 |
MaxQBi | Q91X58 |
PaxDbi | Q91X58 |
PRIDEi | Q91X58 |
PTM databases
iPTMneti | Q91X58 |
PhosphoSitePlusi | Q91X58 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026197, Expressed in granulocyte and 267 other tissues |
Genevisiblei | Q91X58, MM |
Interactioni
Subunit structurei
Binds 'Lys-48'-linked polyubiquitin chains of ubiquitinated proteins (PubMed:18467495, PubMed:24160817, PubMed:26876100). Associates with the proteasome complex; upon exposure to arsenite (PubMed:18467495, PubMed:26876100).
Interacts (via VIM motif) with VCP; the interaction is direct (PubMed:24160817, PubMed:26337389).
Interacts with BAG6 (PubMed:24160817, PubMed:26337389, PubMed:26876100).
Interacts with IGF1R (nascent precursor form) (PubMed:26692333).
Interacts with DERL1, FAF2, NPLOC4 and UFD1; probably through VCP (PubMed:24160817).
5 PublicationsBinary interactionsi
Q91X58
With | #Exp. | IntAct |
---|---|---|
Psma1 [Q9R1P4] | 2 | EBI-15701753,EBI-991653 |
GO - Molecular functioni
- K48-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 213068, 26 interactors |
DIPi | DIP-46091N |
IntActi | Q91X58, 2 interactors |
STRINGi | 10090.ENSMUSP00000027394 |
Miscellaneous databases
RNActi | Q91X58, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q91X58 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 197 – 216 | UIM 1PROSITE-ProRule annotationAdd BLAST | 20 | |
Domaini | 221 – 240 | UIM 2PROSITE-ProRule annotationAdd BLAST | 20 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 141 – 151 | VCP/p97-interacting motif (VIM)1 PublicationAdd BLAST | 11 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 254 – 257 | CAAX motif1 Publication | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 170 – 175 | Poly-Ser | 6 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 52 | AN1-type 1PROSITE-ProRule annotationAdd BLAST | 46 | |
Zinc fingeri | 97 – 145 | AN1-type 2PROSITE-ProRule annotationAdd BLAST | 49 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3183, Eukaryota |
GeneTreei | ENSGT00940000159648 |
InParanoidi | Q91X58 |
OrthoDBi | 1422113at2759 |
PhylomeDBi | Q91X58 |
TreeFami | TF314219 |
Family and domain databases
Gene3Di | 4.10.1110.10, 2 hits |
InterProi | View protein in InterPro IPR035896, AN1-like_Znf IPR003903, UIM_dom IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF01428, zf-AN1, 2 hits |
SMARTi | View protein in SMART SM00726, UIM, 2 hits SM00154, ZnF_AN1, 2 hits |
SUPFAMi | SSF118310, SSF118310, 2 hits |
PROSITEi | View protein in PROSITE PS50330, UIM, 1 hit PS51039, ZF_AN1, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEFPDLGAHC SEPSCQRLDF LPLKCDACSG IFCADHVAYA QHHCGSAYQK
60 70 80 90 100
DIQVPVCPLC NVPVPVARGE PPDRAVGEHI DRDCRSDPAQ QKRKIFTNKC
110 120 130 140 150
ERSGCRQREM MKLTCDRCGR NFCIKHRHPL DHECSGEGHQ TSRAGLAAIS
160 170 180 190 200
RAQGLASTST APSPSRTLPS SSSPSRATPQ LPTRTASPVI ALQNGLSEDE
210 220 230 240 250
ALQRALELSL AEAKPQVLSS QEEDDLALAQ ALSASEAEYQ QQQAQSRSLK
PSNCSLC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WPF5 | A0A087WPF5_MOUSE | AN1-type zinc finger protein 2B | Zfand2b | 255 | Annotation score: | ||
E0CY18 | E0CY18_MOUSE | AN1-type zinc finger protein 2B | Zfand2b | 218 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 174 | P → S in BAB23266 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004332 mRNA Translation: BAB23266.1 AK151430 mRNA Translation: BAE30394.1 BC011495 mRNA Translation: AAH11495.1 |
CCDSi | CCDS15064.1 |
RefSeqi | NP_001153377.1, NM_001159905.1 NP_001153378.1, NM_001159906.1 NP_081122.2, NM_026846.3 |
Genome annotation databases
Ensembli | ENSMUST00000027394; ENSMUSP00000027394; ENSMUSG00000026197 ENSMUST00000160439; ENSMUSP00000125086; ENSMUSG00000026197 |
GeneIDi | 68818 |
KEGGi | mmu:68818 |
UCSCi | uc007bnw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004332 mRNA Translation: BAB23266.1 AK151430 mRNA Translation: BAE30394.1 BC011495 mRNA Translation: AAH11495.1 |
CCDSi | CCDS15064.1 |
RefSeqi | NP_001153377.1, NM_001159905.1 NP_001153378.1, NM_001159906.1 NP_081122.2, NM_026846.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4XKH | X-ray | 3.00 | C/E/H | 187-240 | [»] | |
SMRi | Q91X58 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 213068, 26 interactors |
DIPi | DIP-46091N |
IntActi | Q91X58, 2 interactors |
STRINGi | 10090.ENSMUSP00000027394 |
PTM databases
iPTMneti | Q91X58 |
PhosphoSitePlusi | Q91X58 |
Proteomic databases
EPDi | Q91X58 |
jPOSTi | Q91X58 |
MaxQBi | Q91X58 |
PaxDbi | Q91X58 |
PRIDEi | Q91X58 |
Protocols and materials databases
Antibodypediai | 51845, 180 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027394; ENSMUSP00000027394; ENSMUSG00000026197 ENSMUST00000160439; ENSMUSP00000125086; ENSMUSG00000026197 |
GeneIDi | 68818 |
KEGGi | mmu:68818 |
UCSCi | uc007bnw.2, mouse |
Organism-specific databases
CTDi | 130617 |
MGIi | MGI:1916068, Zfand2b |
Phylogenomic databases
eggNOGi | KOG3183, Eukaryota |
GeneTreei | ENSGT00940000159648 |
InParanoidi | Q91X58 |
OrthoDBi | 1422113at2759 |
PhylomeDBi | Q91X58 |
TreeFami | TF314219 |
Miscellaneous databases
BioGRID-ORCSi | 68818, 0 hits in 17 CRISPR screens |
PROi | PR:Q91X58 |
RNActi | Q91X58, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026197, Expressed in granulocyte and 267 other tissues |
Genevisiblei | Q91X58, MM |
Family and domain databases
Gene3Di | 4.10.1110.10, 2 hits |
InterProi | View protein in InterPro IPR035896, AN1-like_Znf IPR003903, UIM_dom IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF01428, zf-AN1, 2 hits |
SMARTi | View protein in SMART SM00726, UIM, 2 hits SM00154, ZnF_AN1, 2 hits |
SUPFAMi | SSF118310, SSF118310, 2 hits |
PROSITEi | View protein in PROSITE PS50330, UIM, 1 hit PS51039, ZF_AN1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZFN2B_MOUSE | |
Accessioni | Q91X58Primary (citable) accession number: Q91X58 Secondary accession number(s): Q9D0W4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2006 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references