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Protein

Golgi reassembly-stacking protein 1

Gene

Gorasp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Zinc; via pros nitrogenBy similarity1
Metal bindingi19Zinc; via tele nitrogenBy similarity1
Metal bindingi102ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • Golgi organization Source: MGI
  • negative regulation of dendrite morphogenesis Source: UniProtKB
  • protein N-linked glycosylation Source: MGI
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-6807878 COPI-mediated anterograde transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 1
Alternative name(s):
Golgi peripheral membrane protein p65
Golgi reassembly-stacking protein of 65 kDa
Short name:
GRASP65
Gene namesi
Name:Gorasp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1921748 Gorasp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000875712 – 446Golgi reassembly-stacking protein 1Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei216PhosphothreonineCombined sources1
Modified residuei220PhosphothreonineCombined sources1
Modified residuei224PhosphothreonineBy similarity1
Modified residuei365PhosphoserineCombined sources1
Modified residuei367PhosphoserineBy similarity1
Modified residuei376PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking.By similarity
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.By similarity
Myristoylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ91X51
MaxQBiQ91X51
PaxDbiQ91X51
PRIDEiQ91X51

PTM databases

iPTMnetiQ91X51
PhosphoSitePlusiQ91X51

Expressioni

Gene expression databases

BgeeiENSMUSG00000032513 Expressed in 260 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_GORASP1
ExpressionAtlasiQ91X51 baseline and differential
GenevisibleiQ91X51 MM

Interactioni

Subunit structurei

Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. Interacts with TMED2 and TMED3.By similarity

Protein-protein interaction databases

BioGridi216799, 22 interactors
IntActiQ91X51, 26 interactors
STRINGi10090.ENSMUSP00000035099

Structurei

3D structure databases

ProteinModelPortaliQ91X51
SMRiQ91X51
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 104PDZ GRASP-type 1PROSITE-ProRule annotationAdd BLAST91
Domaini110 – 198PDZ GRASP-type 2PROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 214GRASPPROSITE-ProRule annotationAdd BLAST201
Regioni189 – 201Essential for interaction with GOLGA2/GM130By similarityAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi298 – 301Poly-Pro4
Compositional biasi314 – 355Ser-richAdd BLAST42
Compositional biasi351 – 355Poly-Ser5

Sequence similaritiesi

Belongs to the GORASP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3834 Eukaryota
COG5233 LUCA
GeneTreeiENSGT00390000008686
HOGENOMiHOG000054196
HOVERGENiHBG051826
InParanoidiQ91X51
OMAiEVYNIKT
OrthoDBiEOG091G0E90
PhylomeDBiQ91X51
TreeFamiTF314053

Family and domain databases

InterProiView protein in InterPro
IPR024958 GRASP55/65_PDZ
IPR007583 GRASP55_65
IPR036034 PDZ_sf
PANTHERiPTHR12893 PTHR12893, 1 hit
PfamiView protein in Pfam
PF04495 GRASP55_65, 1 hit
SUPFAMiSSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS51865 PDZ_GRASP, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91X51-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLGASSEQP AGGEGFHLHG VQENSPAQQA GLEPYFDFII TIGHSRLNKE
60 70 80 90 100
NDTLKALLKA NVEKPVKLEV FNMKTMKVRE VEVVPSNMWG GQGLLGASVR
110 120 130 140 150
FCSFRRASEH VWHVLDVEPS SPAALAGLCP YTDYIVGSDQ ILQESEDFFT
160 170 180 190 200
LIESHEGKPL KLMVYNSESD SCREVTVTPN AAWGGEGSLG CGIGYGYLHR
210 220 230 240 250
IPTQPSSQHK KPPGATPPGT PATTSQLTAF PLGAPPPWPI PQDSSGPELG
260 270 280 290 300
SRQSDFMEAL PQVPGSFMEG QLLGPGSPSH GAADCGGCLR AMEIPLQPPP
310 320 330 340 350
PVQRVMDPGF LDVSGMSLLD SSNISVCPSL SSSTVLTSTA VSVSGPEDIG
360 370 380 390 400
SSSSSHERGG EATWSGSEFE ISFPDSPGAQ AQADHLPRLT LPDGLTSAAS
410 420 430 440
PEEGLSAELL EAQTEEPADT ASLDCRAETE GRASQAQATP DPEPGL
Length:446
Mass (Da):46,882
Last modified:January 23, 2007 - v3
Checksum:i05A59D996B9AD56B
GO

Sequence cautioni

The sequence BAB30676 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012251 mRNA Translation: AAH12251.1
AK017293 mRNA Translation: BAB30676.1 Different initiation.
CCDSiCCDS23619.1
RefSeqiNP_083252.1, NM_028976.2
UniGeneiMm.104789

Genome annotation databases

EnsembliENSMUST00000035099; ENSMUSP00000035099; ENSMUSG00000032513
GeneIDi74498
KEGGimmu:74498
UCSCiuc009sbq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012251 mRNA Translation: AAH12251.1
AK017293 mRNA Translation: BAB30676.1 Different initiation.
CCDSiCCDS23619.1
RefSeqiNP_083252.1, NM_028976.2
UniGeneiMm.104789

3D structure databases

ProteinModelPortaliQ91X51
SMRiQ91X51
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216799, 22 interactors
IntActiQ91X51, 26 interactors
STRINGi10090.ENSMUSP00000035099

PTM databases

iPTMnetiQ91X51
PhosphoSitePlusiQ91X51

Proteomic databases

EPDiQ91X51
MaxQBiQ91X51
PaxDbiQ91X51
PRIDEiQ91X51

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035099; ENSMUSP00000035099; ENSMUSG00000032513
GeneIDi74498
KEGGimmu:74498
UCSCiuc009sbq.1 mouse

Organism-specific databases

CTDi64689
MGIiMGI:1921748 Gorasp1

Phylogenomic databases

eggNOGiKOG3834 Eukaryota
COG5233 LUCA
GeneTreeiENSGT00390000008686
HOGENOMiHOG000054196
HOVERGENiHBG051826
InParanoidiQ91X51
OMAiEVYNIKT
OrthoDBiEOG091G0E90
PhylomeDBiQ91X51
TreeFamiTF314053

Enzyme and pathway databases

ReactomeiR-MMU-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-6807878 COPI-mediated anterograde transport

Miscellaneous databases

PROiPR:Q91X51
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032513 Expressed in 260 organ(s), highest expression level in urinary bladder urothelium
CleanExiMM_GORASP1
ExpressionAtlasiQ91X51 baseline and differential
GenevisibleiQ91X51 MM

Family and domain databases

InterProiView protein in InterPro
IPR024958 GRASP55/65_PDZ
IPR007583 GRASP55_65
IPR036034 PDZ_sf
PANTHERiPTHR12893 PTHR12893, 1 hit
PfamiView protein in Pfam
PF04495 GRASP55_65, 1 hit
SUPFAMiSSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS51865 PDZ_GRASP, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGORS1_MOUSE
AccessioniPrimary (citable) accession number: Q91X51
Secondary accession number(s): Q9D3L9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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