UniProtKB - Q91X34 (BAAT_MOUSE)
Protein
Bile acid-CoA:amino acid N-acyltransferase
Gene
Baat
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the amidation of bile acids (BAs) with the amino acid taurine (PubMed:9215542). Selective for taurine conjugation of cholyl CoA and only taurine-conjugated BAs are found in bile (PubMed:9215542). Amidation of BAs in the liver with taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism (By similarity). This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption (By similarity). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids (By similarity). In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs (By similarity).By similarity1 Publication
Catalytic activityi
- EC:2.3.1.651 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.1.2.2By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.1.2.27By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.1.2.27By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Kineticsi
- KM=1.9 mM for taurine toward choloyl-CoA1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 235 | Charge relay systemBy similarity | 1 | |
Active sitei | 328 | Charge relay systemBy similarity | 1 | |
Active sitei | 362 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acyl-CoA hydrolase activity Source: GO_Central
- carboxylic ester hydrolase activity Source: UniProtKB-KW
- choloyl-CoA hydrolase activity Source: UniProtKB-EC
- glycine N-choloyltransferase activity Source: MGI
- long-chain acyl-CoA hydrolase activity Source: MGI
- medium-chain acyl-CoA hydrolase activity Source: MGI
- myristoyl-CoA hydrolase activity Source: UniProtKB-EC
- N-acyltransferase activity Source: MGI
- palmitoyl-CoA hydrolase activity Source: UniProtKB-EC
- very long chain acyl-CoA hydrolase activity Source: MGI
GO - Biological processi
- acyl-CoA metabolic process Source: MGI
- animal organ regeneration Source: Ensembl
- bile acid biosynthetic process Source: MGI
- bile acid conjugation Source: MGI
- bile acid metabolic process Source: MGI
- fatty acid metabolic process Source: GO_Central
- glycine metabolic process Source: MGI
- liver development Source: Ensembl
- taurine metabolic process Source: MGI
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Serine esterase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-9033241, Peroxisomal protein import |
SABIO-RKi | Q91X34 |
Protein family/group databases
ESTHERi | mouse-BAAT, Acyl-CoA_Thioesterase |
MEROPSi | S09.A50 |
Names & Taxonomyi
Protein namesi | Recommended name: Bile acid-CoA:amino acid N-acyltransferase (EC:2.3.1.651 Publication)Short name: BACAT Short name: BAT Alternative name(s): |
Gene namesi | Name:Baat |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106642, Baat |
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: UniProtKB
Peroxisome
- peroxisome Source: MGI
Keywords - Cellular componenti
Cytoplasm, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202160 | 1 – 420 | Bile acid-CoA:amino acid N-acyltransferaseAdd BLAST | 420 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 40 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 125 | PhosphoserineBy similarity | 1 | |
Modified residuei | 346 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 350 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 409 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 418 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q91X34 |
MaxQBi | Q91X34 |
PaxDbi | Q91X34 |
PeptideAtlasi | Q91X34 |
PRIDEi | Q91X34 |
PTM databases
iPTMneti | Q91X34 |
PhosphoSitePlusi | Q91X34 |
SwissPalmi | Q91X34 |
Expressioni
Tissue specificityi
Highly expressed in liver, kidney, gallbladder, proximal intestine and distal intestine. Weakly expressed in adrenal gland, lung, brain and muscle.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000039653, Expressed in liver and 105 other tissues |
Genevisiblei | Q91X34, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
IntActi | Q91X34, 3 interactors |
MINTi | Q91X34 |
STRINGi | 10090.ENSMUSP00000041983 |
Miscellaneous databases
RNActi | Q91X34, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the C/M/P thioester hydrolase family.Curated
Phylogenomic databases
eggNOGi | ENOG502QQ8Z, Eukaryota |
GeneTreei | ENSGT01010000222336 |
HOGENOMi | CLU_029849_4_0_1 |
InParanoidi | Q91X34 |
OMAi | LTPFQVV |
OrthoDBi | 1260385at2759 |
PhylomeDBi | Q91X34 |
TreeFami | TF314911 |
Family and domain databases
Gene3Di | 2.60.40.2240, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR016662, Acyl-CoA_thioEstase_long-chain IPR014940, BAAT_C IPR006862, Thio_Ohase/aa_AcTrfase IPR042490, Thio_Ohase/BAAT_N |
Pfami | View protein in Pfam PF08840, BAAT_C, 1 hit PF04775, Bile_Hydr_Trans, 1 hit |
PIRSFi | PIRSF016521, Acyl-CoA_hydro, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
Q91X34-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKLTAVPLS ALVDEPVHIQ VTGLAPFQVV CLQASLKDEK GNLFSSQAFY
60 70 80 90 100
RASEVGEVDL EHDPSLGGDY MGVHPMGLFW SLKPEKLLGR LIKRDVMNSP
110 120 130 140 150
YQIHIKACHP YFPLQDIVVS PPLDSLTLER WYVAPGVKRI QVKESRIRGA
160 170 180 190 200
LFLPPGEGPF PGVIDLFGGA GGLMEFRASL LASRGFATLA LAYWNYDDLP
210 220 230 240 250
SRLEKVDLEY FEEGVEFLLR HPKVLGPGVG ILSVCIGAEI GLSMAINLKQ
260 270 280 290 300
IRATVLINGP NFVSQSPHVY HGQVYPPVPS NEEFVVTNAL GLVEFYRTFQ
310 320 330 340 350
ETADKDSKYC FPIEKAHGHF LFVVGEDDKN LNSKVHANQA IAQLMKNGKK
360 370 380 390 400
NWTLLSYPGA GHLIEPPYTP LCQASRMPIL IPSLSWGGEV IPHAAAQEHS
410 420
WKEIQKFLKQ HLLPDLSSQL
Sequence cautioni
The sequence AAB58325 differs from that shown. Reason: Frameshift.Curated
The sequence BAC25534 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 97 | M → I in AAB58325 (PubMed:9215542).Curated | 1 | |
Sequence conflicti | 117 | I → L in AAB58325 (PubMed:9215542).Curated | 1 | |
Sequence conflicti | 268 | H → Q in BAC25534 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 385 | S → R in BAC25534 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 394 | A → SQ in AAB58325 (PubMed:9215542).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95215 mRNA Translation: AAB58325.1 Frameshift. AK017923 mRNA Translation: BAC25534.1 Frameshift. AL772310 Genomic DNA No translation available. CH466565 Genomic DNA Translation: EDL02319.1 BC012683 mRNA No translation available. |
CCDSi | CCDS18173.1 |
RefSeqi | NP_031545.2, NM_007519.3 |
Genome annotation databases
Ensembli | ENSMUST00000043056; ENSMUSP00000041983; ENSMUSG00000039653 ENSMUST00000166036; ENSMUSP00000129603; ENSMUSG00000039653 |
GeneIDi | 12012 |
KEGGi | mmu:12012 |
UCSCi | uc008svt.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95215 mRNA Translation: AAB58325.1 Frameshift. AK017923 mRNA Translation: BAC25534.1 Frameshift. AL772310 Genomic DNA No translation available. CH466565 Genomic DNA Translation: EDL02319.1 BC012683 mRNA No translation available. |
CCDSi | CCDS18173.1 |
RefSeqi | NP_031545.2, NM_007519.3 |
3D structure databases
SMRi | Q91X34 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q91X34, 3 interactors |
MINTi | Q91X34 |
STRINGi | 10090.ENSMUSP00000041983 |
Protein family/group databases
ESTHERi | mouse-BAAT, Acyl-CoA_Thioesterase |
MEROPSi | S09.A50 |
PTM databases
iPTMneti | Q91X34 |
PhosphoSitePlusi | Q91X34 |
SwissPalmi | Q91X34 |
Proteomic databases
jPOSTi | Q91X34 |
MaxQBi | Q91X34 |
PaxDbi | Q91X34 |
PeptideAtlasi | Q91X34 |
PRIDEi | Q91X34 |
Protocols and materials databases
Antibodypediai | 14641, 196 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043056; ENSMUSP00000041983; ENSMUSG00000039653 ENSMUST00000166036; ENSMUSP00000129603; ENSMUSG00000039653 |
GeneIDi | 12012 |
KEGGi | mmu:12012 |
UCSCi | uc008svt.2, mouse |
Organism-specific databases
CTDi | 570 |
MGIi | MGI:106642, Baat |
Phylogenomic databases
eggNOGi | ENOG502QQ8Z, Eukaryota |
GeneTreei | ENSGT01010000222336 |
HOGENOMi | CLU_029849_4_0_1 |
InParanoidi | Q91X34 |
OMAi | LTPFQVV |
OrthoDBi | 1260385at2759 |
PhylomeDBi | Q91X34 |
TreeFami | TF314911 |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-9033241, Peroxisomal protein import |
SABIO-RKi | Q91X34 |
Miscellaneous databases
BioGRID-ORCSi | 12012, 1 hit in 17 CRISPR screens |
PROi | PR:Q91X34 |
RNActi | Q91X34, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039653, Expressed in liver and 105 other tissues |
Genevisiblei | Q91X34, MM |
Family and domain databases
Gene3Di | 2.60.40.2240, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR016662, Acyl-CoA_thioEstase_long-chain IPR014940, BAAT_C IPR006862, Thio_Ohase/aa_AcTrfase IPR042490, Thio_Ohase/BAAT_N |
Pfami | View protein in Pfam PF08840, BAAT_C, 1 hit PF04775, Bile_Hydr_Trans, 1 hit |
PIRSFi | PIRSF016521, Acyl-CoA_hydro, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAAT_MOUSE | |
Accessioni | Q91X34Primary (citable) accession number: Q91X34 Secondary accession number(s): A2AKK7, O08833, Q8C1I3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families