UniProtKB - Q91X17 (UROM_MOUSE)
Uromodulin
Umod
Functioni
Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia.
By similarityIn the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals.
2 PublicationsGO - Molecular functioni
- calcium ion binding Source: InterPro
- extracellular matrix structural constituent Source: GO_Central
- IgG binding Source: MGI
GO - Biological processi
- apoptotic process Source: MGI
- apoptotic signaling pathway Source: MGI
- autophagy Source: MGI
- calcium ion homeostasis Source: MGI
- cellular calcium ion homeostasis Source: MGI
- cellular chloride ion homeostasis Source: MGI
- cellular phosphate ion homeostasis Source: MGI
- cellular response to unfolded protein Source: MGI
- cellular sodium ion homeostasis Source: MGI
- chaperone-mediated protein folding Source: MGI
- chemical homeostasis Source: MGI
- chloride ion homeostasis Source: MGI
- citric acid secretion Source: MGI
- collecting duct development Source: MGI
- connective tissue replacement Source: MGI
- creatinine homeostasis Source: MGI
- defense response to Gram-negative bacterium Source: MGI
- endoplasmic reticulum organization Source: MGI
- gene expression Source: MGI
- glomerular filtration Source: MGI
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
- inflammatory response Source: MGI
- ion homeostasis Source: UniProtKB
- juxtaglomerular apparatus development Source: MGI
- kidney development Source: MGI
- leukocyte cell-cell adhesion Source: MGI
- lipid metabolic process Source: MGI
- loop of Henle development Source: MGI
- metanephric ascending thin limb development Source: UniProtKB
- metanephric distal convoluted tubule development Source: UniProtKB
- metanephric thick ascending limb development Source: UniProtKB
- micturition Source: MGI
- multicellular organismal homeostasis Source: MGI
- multicellular organismal response to stress Source: MGI
- neutrophil migration Source: MGI
- organ or tissue specific immune response Source: MGI
- peptidyl-threonine phosphorylation Source: MGI
- phosphate ion homeostasis Source: MGI
- potassium ion homeostasis Source: MGI
- protein localization to vacuole Source: MGI
- protein phosphorylation Source: MGI
- protein transport into plasma membrane raft Source: MGI
- regulation of blood pressure Source: MGI
- regulation of protein transport Source: MGI
- regulation of urine volume Source: MGI
- renal sodium ion absorption Source: MGI
- renal urate salt excretion Source: MGI
- renal water homeostasis Source: MGI
- response to endoplasmic reticulum stress Source: MGI
- response to lipopolysaccharide Source: MGI
- response to unfolded protein Source: MGI
- response to water deprivation Source: MGI
- response to xenobiotic stimulus Source: MGI
- RNA splicing Source: MGI
- sodium ion homeostasis Source: MGI
- tumor necrosis factor-mediated signaling pathway Source: MGI
- ubiquitin-dependent ERAD pathway Source: MGI
- urate transport Source: MGI
- urea transmembrane transport Source: MGI
- water homeostasis Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-446203, Asparagine N-linked glycosylation |
Names & Taxonomyi
Protein namesi | Recommended name: UromodulinAlternative name(s): Tamm-Horsfall urinary glycoprotein Short name: THP Cleaved into the following chain: |
Gene namesi | Name:Umod |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102674, Umod |
VEuPathDBi | HostDB:ENSMUSG00000030963 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Note: Detected in urine.1 Publication
Plasma membrane
- Apical cell membrane 1 Publication; GPI-anchor By similarity
- Basolateral cell membrane By similarity; GPI-anchor By similarity
- cilium membrane By similarity
Note: Only a small fraction sorts to the basolateral pole of tubular epithelial cells compared to apical localization. Secreted into urine after cleavage. Colocalizes with NPHP1 and KIF3A.By similarity
Cytoskeleton
- spindle pole Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- basolateral plasma membrane Source: UniProtKB
- ciliary membrane Source: UniProtKB-SubCell
- plasma membrane Source: MGI
Other locations
- anchored component of membrane Source: UniProtKB
- apical part of cell Source: MGI
- cell surface Source: GO_Central
- cilium Source: MGI
- cytoplasmic vesicle Source: MGI
- membrane raft Source: MGI
Keywords - Cellular componenti
Cell membrane, Cell projection, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | By similarityAdd BLAST | 24 | |
ChainiPRO_0000041673 | 25 – 618 | UromodulinAdd BLAST | 594 | |
ChainiPRO_0000407910 | 25 – 588 | Uromodulin, secreted formAdd BLAST | 564 | |
PropeptideiPRO_0000041674 | 619 – 642 | Removed in mature formSequence analysisAdd BLAST | 24 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 25 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 32 ↔ 41 | PROSITE-ProRule annotation | ||
Disulfide bondi | 35 ↔ 50 | PROSITE-ProRule annotation | ||
Glycosylationi | 38 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 52 ↔ 63 | PROSITE-ProRule annotation | ||
Disulfide bondi | 69 ↔ 82 | PROSITE-ProRule annotation | ||
Glycosylationi | 76 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 77 ↔ 91 | PROSITE-ProRule annotation | ||
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 93 ↔ 105 | PROSITE-ProRule annotation | ||
Disulfide bondi | 111 ↔ 125 | PROSITE-ProRule annotation | ||
Disulfide bondi | 119 ↔ 134 | PROSITE-ProRule annotation | ||
Disulfide bondi | 136 ↔ 147 | PROSITE-ProRule annotation | ||
Glycosylationi | 233 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 276 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 298 ↔ 307 | By similarity | ||
Disulfide bondi | 301 ↔ 316 | By similarity | ||
Disulfide bondi | 318 ↔ 348 | By similarity | ||
Glycosylationi | 323 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 336 ↔ 426 | By similarity | ||
Disulfide bondi | 367 ↔ 390 | By similarity | ||
Glycosylationi | 397 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 448 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 507 ↔ 567 | PROSITE-ProRule annotation | ||
Glycosylationi | 514 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 528 ↔ 583 | By similarity | ||
Disulfide bondi | 572 ↔ 579 | By similarity | ||
Lipidationi | 618 | GPI-anchor amidated alanineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 588 – 589 | Cleavage2 Publications | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
CPTACi | non-CPTAC-3626 |
jPOSTi | Q91X17 |
MaxQBi | Q91X17 |
PaxDbi | Q91X17 |
PRIDEi | Q91X17 |
ProteomicsDBi | 297899 |
PTM databases
GlyGeni | Q91X17, 10 sites |
iPTMneti | Q91X17 |
PhosphoSitePlusi | Q91X17 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000030963, Expressed in adult mammalian kidney and 91 other tissues |
Genevisiblei | Q91X17, MM |
Interactioni
Subunit structurei
Homodimer that then polymerizes into long filaments (PubMed:26673890). The filaments can additionally assemble laterally to form a sheet (By similarity).
By similarity1 PublicationProtein-protein interaction databases
IntActi | Q91X17, 1 interactor |
MINTi | Q91X17 |
STRINGi | 10090.ENSMUSP00000033263 |
Miscellaneous databases
RNActi | Q91X17, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 64 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 65 – 106 | EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 107 – 148 | EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 335 – 590 | ZPPROSITE-ProRule annotationAdd BLAST | 256 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 335 – 430 | ZP-NSequence analysisAdd BLAST | 96 | |
Regioni | 431 – 454 | Flexible linker; important for secretion and polymerization into filamentsBy similarityAdd BLAST | 24 | |
Regioni | 455 – 465 | Internal hydrophobic patch (IHP)Sequence analysisAdd BLAST | 11 | |
Regioni | 466 – 590 | ZP-CSequence analysisAdd BLAST | 125 | |
Regioni | 587 – 590 | Essential for cleavage by HPNBy similarity | 4 | |
Regioni | 599 – 608 | Regulates polymerization into filamentsBy similarity | 10 |
Domaini
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QT6B, Eukaryota |
GeneTreei | ENSGT00940000156742 |
HOGENOMi | CLU_028679_0_0_1 |
InParanoidi | Q91X17 |
OMAi | LECGVND |
OrthoDBi | 665331at2759 |
PhylomeDBi | Q91X17 |
TreeFami | TF330284 |
Family and domain databases
Gene3Di | 2.60.40.4100, 1 hit |
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR024731, EGF_dom IPR009030, Growth_fac_rcpt_cys_sf IPR042235, ZP-C IPR001507, ZP_dom IPR017977, ZP_dom_CS |
Pfami | View protein in Pfam PF12947, EGF_3, 1 hit PF07645, EGF_CA, 1 hit PF00100, Zona_pellucida, 1 hit |
PRINTSi | PR00023, ZPELLUCIDA |
SMARTi | View protein in SMART SM00181, EGF, 3 hits SM00179, EGF_CA, 2 hits SM00241, ZP, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS01186, EGF_2, 3 hits PS50026, EGF_3, 3 hits PS01187, EGF_CA, 2 hits PS00682, ZP_1, 1 hit PS51034, ZP_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGIPLTWMLL VMMVTSWFTL AEASNSTEAR RCSECHNNAT CTVDGVVTTC
60 70 80 90 100
SCQTGFTGDG LVCEDMDECA TPWTHNCSNS SCVNTPGSFK CSCQDGFRLT
110 120 130 140 150
PELSCTDVDE CSEQGLSNCH ALATCVNTEG DYLCVCPEGF TGDGWYCECS
160 170 180 190 200
PGSCEPGLDC LPQGPDGKLV CQDPCNTYET LTEYWRSTEY GVGYSCDAGL
210 220 230 240 250
HGWYRFTGQG GVRMAETCVP VLRCNTAAPM WLNGSHPSSS EGIVSRTACA
260 270 280 290 300
HWSDQCCRWS TEIQVKACPG GFYIYNLTAP PECNLAYCTD PSSVEGTCEE
310 320 330 340 350
CRVDEDCISD NGRWRCQCKQ DSNITDVSQL EYRLECGAND IKMSLRKCQL
360 370 380 390 400
QSLGFMNVFM YLNDRQCSGF SESDERDWMS IVTPARNGPC GTVLRRNETH
410 420 430 440 450
ATYSNTLYLA NAIIIRDIII RMNFECSYPL DMKVSLKTSL QPMVSALNIS
460 470 480 490 500
LGGTGKFTVR MALFQSPTYT QPHQGPSVML STEAFLYVGT MLDGGDLSRF
510 520 530 540 550
VLLMTNCYAT PSSNSTDPVK YFIIQDSCPR TEDTTIQVTE NGESSQARFS
560 570 580 590 600
VQMFRFAGNY DLVYLHCEVY LCDSTSEQCK PTCSGTRFRS GNFIDQTRVL
610 620 630 640
NLGPITRQGV QASVSKAASS NLRLLSIWLL LFPSATLIFM VQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LI10 | A0A140LI10_MOUSE | Uromodulin | Umod | 187 | Annotation score: | ||
A0A140LJ95 | A0A140LJ95_MOUSE | Uromodulin | Umod | 87 | Annotation score: | ||
A0A140LJ15 | A0A140LJ15_MOUSE | Uromodulin | Umod | 77 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | E → G in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 102 – 104 | ELS → GLG in AAA73896 (PubMed:7873609).Curated | 3 | |
Sequence conflicti | 138 | E → K in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 152 | G → S in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 200 | L → Q in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 223 | R → A in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 255 | Q → H in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 279 | A → E in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 340 | D → G in BAE38225 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 473 | H → Y in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 590 | S → C in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 606 | T → TRQ in AAA73896 (PubMed:7873609).Curated | 1 | |
Sequence conflicti | 616 – 617 | Missing in AAA73896 (PubMed:7873609).Curated | 2 | |
Sequence conflicti | 633 | P → L in AAA73896 (PubMed:7873609).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33406 mRNA Translation: AAA73896.1 AK085460 mRNA Translation: BAC39452.1 AK144065 mRNA Translation: BAE25681.1 AK164688 mRNA Translation: BAE37877.1 AK165507 mRNA Translation: BAE38225.1 BC012973 mRNA Translation: AAH12973.1 |
CCDSi | CCDS21780.1 |
PIRi | S52111 |
RefSeqi | NP_001265534.1, NM_001278605.1 NP_033496.1, NM_009470.5 |
Genome annotation databases
Ensembli | ENSMUST00000033263; ENSMUSP00000033263; ENSMUSG00000030963 ENSMUST00000209095; ENSMUSP00000146652; ENSMUSG00000030963 |
GeneIDi | 22242 |
KEGGi | mmu:22242 |
UCSCi | uc009jlb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L33406 mRNA Translation: AAA73896.1 AK085460 mRNA Translation: BAC39452.1 AK144065 mRNA Translation: BAE25681.1 AK164688 mRNA Translation: BAE37877.1 AK165507 mRNA Translation: BAE38225.1 BC012973 mRNA Translation: AAH12973.1 |
CCDSi | CCDS21780.1 |
PIRi | S52111 |
RefSeqi | NP_001265534.1, NM_001278605.1 NP_033496.1, NM_009470.5 |
3D structure databases
AlphaFoldDBi | Q91X17 |
SMRi | Q91X17 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q91X17, 1 interactor |
MINTi | Q91X17 |
STRINGi | 10090.ENSMUSP00000033263 |
PTM databases
GlyGeni | Q91X17, 10 sites |
iPTMneti | Q91X17 |
PhosphoSitePlusi | Q91X17 |
Proteomic databases
CPTACi | non-CPTAC-3626 |
jPOSTi | Q91X17 |
MaxQBi | Q91X17 |
PaxDbi | Q91X17 |
PRIDEi | Q91X17 |
ProteomicsDBi | 297899 |
Protocols and materials databases
Antibodypediai | 4003, 567 antibodies from 38 providers |
DNASUi | 22242 |
Genome annotation databases
Ensembli | ENSMUST00000033263; ENSMUSP00000033263; ENSMUSG00000030963 ENSMUST00000209095; ENSMUSP00000146652; ENSMUSG00000030963 |
GeneIDi | 22242 |
KEGGi | mmu:22242 |
UCSCi | uc009jlb.2, mouse |
Organism-specific databases
CTDi | 7369 |
MGIi | MGI:102674, Umod |
VEuPathDBi | HostDB:ENSMUSG00000030963 |
Phylogenomic databases
eggNOGi | ENOG502QT6B, Eukaryota |
GeneTreei | ENSGT00940000156742 |
HOGENOMi | CLU_028679_0_0_1 |
InParanoidi | Q91X17 |
OMAi | LECGVND |
OrthoDBi | 665331at2759 |
PhylomeDBi | Q91X17 |
TreeFami | TF330284 |
Enzyme and pathway databases
Reactomei | R-MMU-446203, Asparagine N-linked glycosylation |
Miscellaneous databases
BioGRID-ORCSi | 22242, 3 hits in 72 CRISPR screens |
ChiTaRSi | Umod, mouse |
PROi | PR:Q91X17 |
RNActi | Q91X17, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030963, Expressed in adult mammalian kidney and 91 other tissues |
Genevisiblei | Q91X17, MM |
Family and domain databases
Gene3Di | 2.60.40.4100, 1 hit |
InterProi | View protein in InterPro IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR024731, EGF_dom IPR009030, Growth_fac_rcpt_cys_sf IPR042235, ZP-C IPR001507, ZP_dom IPR017977, ZP_dom_CS |
Pfami | View protein in Pfam PF12947, EGF_3, 1 hit PF07645, EGF_CA, 1 hit PF00100, Zona_pellucida, 1 hit |
PRINTSi | PR00023, ZPELLUCIDA |
SMARTi | View protein in SMART SM00181, EGF, 3 hits SM00179, EGF_CA, 2 hits SM00241, ZP, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 2 hits PS01186, EGF_2, 3 hits PS50026, EGF_3, 3 hits PS01187, EGF_CA, 2 hits PS00682, ZP_1, 1 hit PS51034, ZP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UROM_MOUSE | |
Accessioni | Q91X17Primary (citable) accession number: Q91X17 Secondary accession number(s): Q3TN64, Q3TP60, Q62285 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot