UniProtKB - Q91WU6 (ZDHC7_MOUSE)
Protein
Palmitoyltransferase ZDHHC7
Gene
Zdhhc7
Organism
Mus musculus (Mouse)
Status
Functioni
Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and therefore functions in several unrelated biological processes (PubMed:15603741, PubMed:19001095, PubMed:23687301, PubMed:25253725). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (Probable). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane and their function in rapid intracellular signaling upon binding of sex hormones. Palmitoylates GNAQ, a heterotrimeric G protein, regulating its dynamic localization at the plasma membrane and is thereby involved in GNAQ-dependent G protein-coupled receptor signaling pathways (PubMed:19001095). Functions also in ligand-induced cell death by regulating the FAS signaling pathway through the palmitoylation and stabilization of the receptor at the plasma membrane. In epithelial cells, palmitoylates SCRIB and regulates its localization to the plasma membrane, regulating indirectly cell polarity and differentiation. Also palmitoylates JAM3 and promotes its expression at tight junctions and regulates its function in cell migration (By similarity). Palmitoylates the glucose transporter GLUT4/SLC2A4 and controls the insulin-dependent translocation of GLUT4 to the plasma membrane (PubMed:28057756). In brain, could also palmitoylate SNAP25 and DLG4/PSD95 (PubMed:15603741, PubMed:25253725). Could also palmitoylate DNAJC5 and regulate its localization to the Golgi membrane (PubMed:18596047). Could also palmitoylate NCDN (PubMed:23687301). May play a role in follicle stimulation hormone (FSH) activation of testicular Sertoli cells (By similarity).By similarity1 Publication6 Publications
Catalytic activityi
- EC:2.3.1.2256 PublicationsThis reaction proceeds in the forward1 Publication direction.
- L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 160 | S-palmitoyl cysteine intermediatePROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: MGI
- protein-cysteine S-myristoyltransferase activity Source: UniProtKB
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB
- protein-cysteine S-stearoyltransferase activity Source: UniProtKB
GO - Biological processi
- glucose import in response to insulin stimulus Source: UniProtKB
- negative regulation of catabolic process Source: UniProtKB
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- polarized epithelial cell differentiation Source: UniProtKB
- protein palmitoylation Source: UniProtKB
- protein targeting to membrane Source: GO_Central
- regulation of Fas signaling pathway Source: UniProtKB
- regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- regulation of protein localization to cell-cell junction Source: UniProtKB
- regulation of protein localization to plasma membrane Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-9009391, Extra-nuclear estrogen signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC7Curated (EC:2.3.1.2256 Publications)Alternative name(s): Acyltransferase ZDHHC71 Publication (EC:2.3.1.-1 Publication) GABA-A receptor-associated membrane protein 2Imported SERZ-beta1 Publication Zinc finger DHHC domain-containing protein 7Imported Short name: DHHC-71 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2142662, Zdhhc7 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 50 | CytoplasmicCuratedAdd BLAST | 50 | |
Transmembranei | 51 – 71 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 72 – 75 | LumenalCurated | 4 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 173 | CytoplasmicCuratedAdd BLAST | 77 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 195 – 217 | LumenalCuratedAdd BLAST | 23 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 308 | CytoplasmicCuratedAdd BLAST | 70 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Homozygous knockout mice are viable, fertile and do not display overt phenotype (PubMed:27875292). Knockout mice are hyperglycemic, glucose intolerant and develop a type II diabetic syndrome (PubMed:28057756). Zdhhc3 and Zdhhc7 double knockout mice show a perinatally lethal phenotype (PubMed:27875292).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 – 158 | DH → AA: Fails to enhance DLG4 palmitoylation. 1 Publication | 2 | |
Mutagenesisi | 160 | C → S: Loss of protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212875 | 1 – 308 | Palmitoyltransferase ZDHHC7Add BLAST | 308 |
Post-translational modificationi
Autopalmitoylated.2 Publications
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
MaxQBi | Q91WU6 |
PaxDbi | Q91WU6 |
PRIDEi | Q91WU6 |
PTM databases
iPTMneti | Q91WU6 |
PhosphoSitePlusi | Q91WU6 |
SwissPalmi | Q91WU6 |
Expressioni
Tissue specificityi
Ubiquitously expressed, with highest levels in liver, kidney and brain. Expressed in all brain regions.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031823, Expressed in intestine and 285 other tissues |
Genevisiblei | Q91WU6, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034280 |
Miscellaneous databases
RNActi | Q91WU6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 130 – 180 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Belongs to the DHHC palmitoyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156519 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q91WU6 |
OMAi | ATKEYMD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q91WU6 |
TreeFami | TF319798 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q91WU6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPSGHRLRD IEHHPLLTDN DNYDSASSSS SETDMADRVW FIRDGCGMVC
60 70 80 90 100
AVMTWLLVVY ADFVVTFVML LPSKDFWYSV VNGVLFNCLA VLALSSHLRT
110 120 130 140 150
MLTDPGAVPK GNATKEYMES LQLKPGEVIY KCPKCCCIKP ERAHHCSICK
160 170 180 190 200
RCIRKMDHHC PWVNNCVGEK NQRFFVLFTM YIALSSVHAL ILCGLQFISC
210 220 230 240 250
VRGQWTECSD FSPPITVILL VFLCLEGLLF FTFTAVMFGT QIHSICNDET
260 270 280 290 300
EIERLKSEKP TWERRLRWEG MKSVFGGPPS LLWMNPFVGF RLRRLQMRTR
KGGPEFSV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 | L → F in BAE42116 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166673 mRNA Translation: AAO27360.1 AK041905 mRNA Translation: BAC31093.1 AK157968 mRNA Translation: BAE34289.1 AK170639 mRNA Translation: BAE41929.1 AK170923 mRNA Translation: BAE42116.1 AK172462 mRNA Translation: BAE43018.1 BC013467 mRNA Translation: AAH13467.1 BC029666 mRNA Translation: AAH29666.1 BC075666 mRNA Translation: AAH75666.1 |
CCDSi | CCDS22715.1 |
RefSeqi | NP_598728.1, NM_133967.3 XP_011246559.1, XM_011248257.2 |
Genome annotation databases
Ensembli | ENSMUST00000034280; ENSMUSP00000034280; ENSMUSG00000031823 |
GeneIDi | 102193 |
KEGGi | mmu:102193 |
UCSCi | uc009nqr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166673 mRNA Translation: AAO27360.1 AK041905 mRNA Translation: BAC31093.1 AK157968 mRNA Translation: BAE34289.1 AK170639 mRNA Translation: BAE41929.1 AK170923 mRNA Translation: BAE42116.1 AK172462 mRNA Translation: BAE43018.1 BC013467 mRNA Translation: AAH13467.1 BC029666 mRNA Translation: AAH29666.1 BC075666 mRNA Translation: AAH75666.1 |
CCDSi | CCDS22715.1 |
RefSeqi | NP_598728.1, NM_133967.3 XP_011246559.1, XM_011248257.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034280 |
PTM databases
iPTMneti | Q91WU6 |
PhosphoSitePlusi | Q91WU6 |
SwissPalmi | Q91WU6 |
Proteomic databases
MaxQBi | Q91WU6 |
PaxDbi | Q91WU6 |
PRIDEi | Q91WU6 |
Protocols and materials databases
Antibodypediai | 30621, 135 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034280; ENSMUSP00000034280; ENSMUSG00000031823 |
GeneIDi | 102193 |
KEGGi | mmu:102193 |
UCSCi | uc009nqr.1, mouse |
Organism-specific databases
CTDi | 55625 |
MGIi | MGI:2142662, Zdhhc7 |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156519 |
HOGENOMi | CLU_048061_1_1_1 |
InParanoidi | Q91WU6 |
OMAi | ATKEYMD |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q91WU6 |
TreeFami | TF319798 |
Enzyme and pathway databases
Reactomei | R-MMU-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 102193, 0 hits in 17 CRISPR screens |
PROi | PR:Q91WU6 |
RNActi | Q91WU6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031823, Expressed in intestine and 285 other tissues |
Genevisiblei | Q91WU6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDHC7_MOUSE | |
Accessioni | Q91WU6Primary (citable) accession number: Q91WU6 Secondary accession number(s): Q3TC41, Q6EMK2, Q8K1H6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families