UniProtKB - Q91WS2 (NLRP6_MOUSE)
NACHT, LRR and PYD domains-containing protein 6
Nlrp6
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 200 – 207 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- peptide binding Source: MGI
- vasopressin receptor activity Source: UniProtKB
GO - Biological processi
- inflammatory response Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- negative regulation of ERK1 and ERK2 cascade Source: CACAO
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: CACAO
- negative regulation of immune response Source: MGI
- negative regulation of inflammatory response to antigenic stimulus Source: CACAO
- negative regulation of MAPK cascade Source: MGI
- negative regulation of toll-like receptor signaling pathway Source: MGI
- regulation of autophagy Source: MGI
- regulation of inflammatory response Source: UniProtKB
- regulation of mucus secretion Source: MGI
- response to bacterium Source: MGI
- wound healing Source: MGI
Keywordsi
Biological process | Immunity, Inflammatory response, Innate immunity |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: NACHT, LRR and PYD domains-containing protein 6Alternative name(s): Angiotensin II/vasopressin receptor Non-angiotensin-vasopressin receptor1 Publication Short name: Non-AVR1 Publication PYRIN-containing APAF1-like protein 5-like |
Gene namesi | Name:Nlrp6 Synonyms:Nalp6, Navr, Pypaf5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141990, Nlrp6 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytosol
- Inflammasome By similarity
Nucleus
- Nucleus membrane By similarity
Other locations
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Other locations
- Cytoplasm By similarity
Note: Predominantly expressed in the cell membrane.By similarity
Cytosol
- inflammasome complex Source: UniProtKB-SubCell
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Inflammasome, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080893 | 1 – 869 | NACHT, LRR and PYD domains-containing protein 6Add BLAST | 869 |
Proteomic databases
PaxDbi | Q91WS2 |
PeptideAtlasi | Q91WS2 |
PRIDEi | Q91WS2 |
PTM databases
PhosphoSitePlusi | Q91WS2 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000038745, Expressed in liver and 81 other tissues |
Genevisiblei | Q91WS2, MM |
Interactioni
Subunit structurei
Sensor component of NLRP6 inflammasomes. Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation. The core of NLRP6 inflammasomes consists of a signal sensor component (NLRP6), an adapter (ASC/PYCARD), which recruits an effector proinflammatory caspase (CASP1 and, possibly, CASP4/CASP11). Within the complex, NLRP6 and PYCARD interact via their respective pyrin domains. This interaction initiates speck formation (nucleation) which greatly enhances further addition of soluble PYCARD molecules to the speck in a prion-like polymerization process. NLRP6 localizes at the end of each PYCARD filament. Clustered PYCARD nucleates the formation of CASP1 filaments through the interaction of their respective CARD domains, acting as a platform for CASP1 polymerization. CASP1 filament formation increases local enzyme concentration, resulting in trans-autocleavage and activation. Active CASP1 then processes IL1B and IL18 precursors, leading to the release of mature cytokines in the extracellular milieu and inflammatory response. In this complex, the sensor protein is sub-stoichiometric to PYCARD, and PYCARD is further substoichiometric to CASP1, suggesting amplifications of signal transduction from the sensor, via the adapter, to the effector.
By similarityBinary interactionsi
Hide detailsQ91WS2
With | #Exp. | IntAct |
---|---|---|
Dhx15 [O35286] | 2 | EBI-16182145,EBI-8322087 |
Isoform 1 [Q91WS2-1]
With | #Exp. | IntAct |
---|---|---|
DHX15 [O43143] from Homo sapiens. | 2 | EBI-16182226,EBI-1237044 |
MAVS - isoform 1 [Q7Z434-1] from Homo sapiens. | 2 | EBI-16182226,EBI-15577799 |
Protein-protein interaction databases
DIPi | DIP-61874N |
IntActi | Q91WS2, 3 interactors |
STRINGi | 10090.ENSMUSP00000139170 |
Miscellaneous databases
RNActi | Q91WS2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 128 | PyrinPROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 194 – 511 | NACHTPROSITE-ProRule annotationAdd BLAST | 318 | |
Repeati | 460 – 485 | LRR 1Add BLAST | 26 | |
Repeati | 635 – 658 | LRR 2Add BLAST | 24 | |
Repeati | 837 – 860 | LRR 3Add BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 595 – 611 | Asp/Glu-richAdd BLAST | 17 | |
Compositional biasi | 680 – 687 | Poly-Lys | 8 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | ENOG502SANB, Eukaryota |
GeneTreei | ENSGT00940000162758 |
HOGENOMi | CLU_002274_2_2_1 |
InParanoidi | Q91WS2 |
OMAi | GLAWPRC |
OrthoDBi | 114368at2759 |
PhylomeDBi | Q91WS2 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR032675, LRR_dom_sf IPR007111, NACHT_NTPase IPR041267, NLRP_HD2 IPR041075, NOD2_WH IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05729, NACHT, 1 hit PF17776, NLRC4_HD2, 1 hit PF17779, NOD2_WH, 1 hit PF02758, PYRIN, 1 hit |
SMARTi | View protein in SMART SM01289, PYRIN, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50824, DAPIN, 1 hit PS50837, NACHT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDAAGASCSS VDAVARELLM ATLEELSQEQ LKRFRHKLRD APLDGRSIPW
60 70 80 90 100
GRLERSDAVD LVDKLIEFYE PVPAVEMTRQ VLKRSDIRDV ASRLKQQQLQ
110 120 130 140 150
KLGPTSVLLS VSAFKKKYRE HVLRQHAKVK ERNARSVKIN KRFTKLLIAP
160 170 180 190 200
GTGAVEDELL GPLGEPEPER ARRSDTHTFN RLFRGNDEES SQPLTVVLQG
210 220 230 240 250
PAGIGKTMAA KKILYDWAAG KLYHSQVDFA FFMPCGELLE RPGKRSLADL
260 270 280 290 300
VLDQCPDRAW PVKRILAQPN RLLFILDGAD ELPTLPSSEA TPCKDPLEAT
310 320 330 340 350
SGLRVLSGLL SQELLPGARL LVTTRHAATG RLQGRLCSPQ CAEIRGFSDK
360 370 380 390 400
DKKKYFFKFF RDERKAERAY RFVKENETLF ALCFVPFVCW IVCTVLQQQL
410 420 430 440 450
ELGRDLSRTS KTTTSVYLLF ITSMLKSAGT NGPRVQGELR TLCRLAREGI
460 470 480 490 500
LDHHKAQFSE EDLEKLKLRG SQVQTIFLNK KEIPGVLKTE VTYQFIDQSF
510 520 530 540 550
QEFLAALSYL LEAERTPGTP AGGVQKLLNS DAELRGHLAL TTRFLFGLLN
560 570 580 590 600
TEGLRDIGNH FGCVVPDHVK KDTLRWVQGQ SHPKGPPVGA KKTAELEDIE
610 620 630 640 650
DAEEEEEEEE DLNFGLELLY CLYETQEEDF VRQALSSLPE IVLERVRLTR
660 670 680 690 700
MDLEVLNYCV QCCPDGQALR LVSCGLVAAK EKKKKKKSLV KRLKGSQSTK
710 720 730 740 750
KQPPVSLLRP LCETMTTPKC HLSVLILSHC RLPDAVCRDL SEALKVAPAL
760 770 780 790 800
RELGLLQSRL TNTGLRLLCE GLAWPKCQVK TLRMQLPDLQ EVINYLVIVL
810 820 830 840 850
QQSPVLTTLD LSGCQLPGVI VEPLCAALKH PKCSLKTLSL TSVELSENSL
860
RDLQAVKTSK PDLSIIYSK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9QNS0 | E9QNS0_MOUSE | NACHT, LRR and PYD domains-containi... | Nlrp6 | 882 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 429 | G → F in ACN32212 (PubMed:20923861).Curated | 1 | |
Sequence conflicti | 454 | Missing in ACN32212 (PubMed:20923861).Curated | 1 | |
Sequence conflicti | 733 | P → R in ACN32212 (PubMed:20923861).Curated | 1 | |
Sequence conflicti | 869 | K → KIRHTSPASEG in ACN32212 (PubMed:20923861).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042301 | 1 – 423 | Missing in isoform 2. 2 PublicationsAdd BLAST | 423 | |
Alternative sequenceiVSP_057971 | 1 – 9 | MDAAGASCS → MYCPGAPCGECAMCDPGCFG GSGTGPIQVASVMITLLIP in isoform 3. | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JF810536 mRNA Translation: AEI54131.1 AC107815 Genomic DNA No translation available. AC162287 Genomic DNA No translation available. BC013519 mRNA Translation: AAH13519.1 BC031139 mRNA Translation: AAH31139.1 FJ222825 mRNA Translation: ACN32212.1 Frameshift. AY355343 mRNA Translation: AAR14740.1 |
CCDSi | CCDS40178.2 [Q91WS2-1] |
RefSeqi | NP_598707.2, NM_133946.2 [Q91WS2-1] XP_006536212.1, XM_006536149.3 [Q91WS2-3] |
Genome annotation databases
Ensembli | ENSMUST00000183845; ENSMUSP00000139357; ENSMUSG00000038745 [Q91WS2-1] ENSMUST00000184560; ENSMUSP00000139170; ENSMUSG00000038745 [Q91WS2-3] |
GeneIDi | 101613 |
KEGGi | mmu:101613 |
UCSCi | uc009kiv.3, mouse [Q91WS2-1] uc029wpm.2, mouse |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JF810536 mRNA Translation: AEI54131.1 AC107815 Genomic DNA No translation available. AC162287 Genomic DNA No translation available. BC013519 mRNA Translation: AAH13519.1 BC031139 mRNA Translation: AAH31139.1 FJ222825 mRNA Translation: ACN32212.1 Frameshift. AY355343 mRNA Translation: AAR14740.1 |
CCDSi | CCDS40178.2 [Q91WS2-1] |
RefSeqi | NP_598707.2, NM_133946.2 [Q91WS2-1] XP_006536212.1, XM_006536149.3 [Q91WS2-3] |
3D structure databases
SMRi | Q91WS2 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-61874N |
IntActi | Q91WS2, 3 interactors |
STRINGi | 10090.ENSMUSP00000139170 |
PTM databases
PhosphoSitePlusi | Q91WS2 |
Proteomic databases
PaxDbi | Q91WS2 |
PeptideAtlasi | Q91WS2 |
PRIDEi | Q91WS2 |
Protocols and materials databases
Antibodypediai | 58845, 223 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000183845; ENSMUSP00000139357; ENSMUSG00000038745 [Q91WS2-1] ENSMUST00000184560; ENSMUSP00000139170; ENSMUSG00000038745 [Q91WS2-3] |
GeneIDi | 101613 |
KEGGi | mmu:101613 |
UCSCi | uc009kiv.3, mouse [Q91WS2-1] uc029wpm.2, mouse |
Organism-specific databases
CTDi | 171389 |
MGIi | MGI:2141990, Nlrp6 |
Phylogenomic databases
eggNOGi | ENOG502SANB, Eukaryota |
GeneTreei | ENSGT00940000162758 |
HOGENOMi | CLU_002274_2_2_1 |
InParanoidi | Q91WS2 |
OMAi | GLAWPRC |
OrthoDBi | 114368at2759 |
PhylomeDBi | Q91WS2 |
Miscellaneous databases
BioGRID-ORCSi | 101613, 0 hits in 17 CRISPR screens |
PROi | PR:Q91WS2 |
RNActi | Q91WS2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038745, Expressed in liver and 81 other tissues |
Genevisiblei | Q91WS2, MM |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR032675, LRR_dom_sf IPR007111, NACHT_NTPase IPR041267, NLRP_HD2 IPR041075, NOD2_WH IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05729, NACHT, 1 hit PF17776, NLRC4_HD2, 1 hit PF17779, NOD2_WH, 1 hit PF02758, PYRIN, 1 hit |
SMARTi | View protein in SMART SM01289, PYRIN, 1 hit |
SUPFAMi | SSF47986, SSF47986, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50824, DAPIN, 1 hit PS50837, NACHT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NLRP6_MOUSE | |
Accessioni | Q91WS2Primary (citable) accession number: Q91WS2 Secondary accession number(s): C9W978 Q8K0L4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | August 21, 2007 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families