UniProtKB - Q91WP0 (MASP2_MOUSE)
Protein
Mannan-binding lectin serine protease 2
Gene
Masp2
Organism
Mus musculus (Mouse)
Status
Functioni
Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase (By similarity).By similarity
Miscellaneous
Dimerization and MBL2 binding requires calcium ions.By similarity
Catalytic activityi
- Selective cleavage after Arg-223 in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile). EC:3.4.21.104
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 67 | Calcium 1By similarity | 1 | |
Metal bindingi | 75 | Calcium 1By similarity | 1 | |
Metal bindingi | 120 | Calcium 1By similarity | 1 | |
Metal bindingi | 122 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 123 | Calcium 1By similarity | 1 | |
Metal bindingi | 138 | Calcium 2By similarity | 1 | |
Metal bindingi | 141 | Calcium 2By similarity | 1 | |
Metal bindingi | 158 | Calcium 2By similarity | 1 | |
Metal bindingi | 162 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 483 | Charge relay systemBy similarity | 1 | |
Active sitei | 532 | Charge relay systemBy similarity | 1 | |
Active sitei | 632 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- calcium ion binding Source: InterPro
- complement component C4b binding Source: MGI
- peptidase activity Source: MGI
- serine-type endopeptidase activity Source: MGI
GO - Biological processi
- complement activation, classical pathway Source: UniProtKB-KW
- complement activation, lectin pathway Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Complement pathway, Immunity, Innate immunity |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.21.104, 3474 |
Reactomei | R-MMU-166662, Lectin pathway of complement activation R-MMU-166663, Initial triggering of complement R-MMU-2855086, Ficolins bind to repetitive carbohydrate structures on the target cell surface |
Protein family/group databases
MEROPSi | S01.229 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannan-binding lectin serine protease 2 (EC:3.4.21.104)Alternative name(s): MBL-associated serine protease 2 Mannose-binding protein-associated serine protease 2 Short name: MASP-2 Cleaved into the following 2 chains: |
Gene namesi | Name:Masp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1330832, Masp2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000027601 | 20 – 685 | Mannan-binding lectin serine protease 2Add BLAST | 666 | |
ChainiPRO_0000027602 | 20 – 443 | Mannan-binding lectin serine protease 2 A chainAdd BLAST | 424 | |
ChainiPRO_0000027603 | 444 – 685 | Mannan-binding lectin serine protease 2 B chainAdd BLAST | 242 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 72 ↔ 90 | By similarity | ||
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 142 ↔ 156 | By similarity | ||
Disulfide bondi | 152 ↔ 165 | By similarity | ||
Modified residuei | 158 | (3R)-3-hydroxyasparagineSequence analysis | 1 | |
Disulfide bondi | 167 ↔ 180 | By similarity | ||
Disulfide bondi | 184 ↔ 211 | By similarity | ||
Disulfide bondi | 241 ↔ 259 | By similarity | ||
Glycosylationi | 285 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 300 ↔ 348 | By similarity | ||
Glycosylationi | 308 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 328 ↔ 361 | By similarity | ||
Disulfide bondi | 366 ↔ 411 | By similarity | ||
Disulfide bondi | 396 ↔ 429 | By similarity | ||
Disulfide bondi | 433 ↔ 552 | Interchain (between A and B chains)PROSITE-ProRule annotation | ||
Glycosylationi | 545 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 598 ↔ 617 | By similarity | ||
Disulfide bondi | 628 ↔ 659 | By similarity | ||
Glycosylationi | 641 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 443 – 444 | CleavageBy similarity | 2 |
Keywords - PTMi
Autocatalytic cleavage, Disulfide bond, Glycoprotein, HydroxylationProteomic databases
CPTACi | non-CPTAC-3430 non-CPTAC-3722 |
PaxDbi | Q91WP0 |
PeptideAtlasi | Q91WP0 |
PRIDEi | Q91WP0 |
PTM databases
GlyGeni | Q91WP0, 5 sites |
iPTMneti | Q91WP0 |
PhosphoSitePlusi | Q91WP0 |
Expressioni
Tissue specificityi
Plasma.
Gene expression databases
Bgeei | ENSMUSG00000028979, Expressed in liver and 108 other tissues |
Genevisiblei | Q91WP0, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked. Binds MBL2. Isoform 2 binds to MASP1. Binds SERPING1 (By similarity).
By similarityGO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- complement component C4b binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201317, 1 interactor |
STRINGi | 10090.ENSMUSP00000049729 |
Miscellaneous databases
RNActi | Q91WP0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 137 | CUB 1PROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 138 – 181 | EGF-like; calcium-bindingAdd BLAST | 44 | |
Domaini | 184 – 296 | CUB 2PROSITE-ProRule annotationAdd BLAST | 113 | |
Domaini | 298 – 363 | Sushi 1PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 364 – 431 | Sushi 2PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 444 – 683 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 240 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
EGF-like domain, Repeat, Signal, SushiPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00950000183084 |
HOGENOMi | CLU_103588_3_0_1 |
InParanoidi | Q91WP0 |
OMAi | QWPKPVF |
OrthoDBi | 156878at2759 |
PhylomeDBi | Q91WP0 |
TreeFami | TF330373 |
Family and domain databases
CDDi | cd00033, CCP, 2 hits cd00041, CUB, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR037571, MASP2 IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR035914, Sperma_CUB_dom_sf IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24255:SF10, PTHR24255:SF10, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF07645, EGF_CA, 1 hit PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 2 hits SM00042, CUB, 2 hits SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57535, SSF57535, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01180, CUB, 2 hits PS01186, EGF_2, 1 hit PS01187, EGF_CA, 1 hit PS50923, SUSHI, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q91WP0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRLLIFLGLL WSLVATLLGS KWPEPVFGRL VSPGFPEKYA DHQDRSWTLT
60 70 80 90 100
APPGYRLRLY FTHFDLELSY RCEYDFVKLS SGTKVLATLC GQESTDTEQA
110 120 130 140 150
PGNDTFYSLG PSLKVTFHSD YSNEKPFTGF EAFYAAEDVD ECRVSLGDSV
160 170 180 190 200
PCDHYCHNYL GGYYCSCRAG YVLHQNKHTC SALCSGQVFT GRSGYLSSPE
210 220 230 240 250
YPQPYPKLSS CTYSIRLEDG FSVILDFVES FDVETHPEAQ CPYDSLKIQT
260 270 280 290 300
DKGEHGPFCG KTLPPRIETD SHKVTITFAT DESGNHTGWK IHYTSTARPC
310 320 330 340 350
PDPTAPPNGS ISPVQAIYVL KDRFSVFCKT GFELLQGSVP LKSFTAVCQK
360 370 380 390 400
DGSWDRPMPE CSIIDCGPPD DLPNGHVDYI TGPEVTTYKA VIQYSCEETF
410 420 430 440 450
YTMSSNGKYV CEADGFWTSS KGEKLPPVCE PVCGLSTHTI GGRIVGGQPA
460 470 480 490 500
KPGDFPWQVL LLGQTTAAAG ALIHDNWVLT AAHAVYEKRM AASSLNIRMG
510 520 530 540 550
ILKRLSPHYT QAWPEEIFIH EGYTHGAGFD NDIALIKLKN KVTINGSIMP
560 570 580 590 600
VCLPRKEAAS LMRTDFTGTV AGWGLTQKGL LARNLMFVDI PIADHQKCTA
610 620 630 640 650
VYEKLYPGVR VSANMLCAGL ETGGKDSCRG DSGGALVFLD NETQRWFVGG
660 670 680
IVSWGSINCG AADQYGVYTK VINYIPWIEN IISNF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 2 | MR → MSLPCPQ (PubMed:10586086).Curated | 2 | |
Sequence conflicti | 172 | V → I in BAA34674 (PubMed:9794427).Curated | 1 | |
Sequence conflicti | 172 | V → I in CAB65247 (PubMed:10586086).Curated | 1 | |
Sequence conflicti | 192 | R → K in CAB65250 (PubMed:10586086).Curated | 1 | |
Sequence conflicti | 317 | I → T in BAA34674 (PubMed:9794427).Curated | 1 | |
Sequence conflicti | 325 | S → Y in CAB65250 (PubMed:10586086).Curated | 1 | |
Sequence conflicti | 384 | E → Q in BAA34674 (PubMed:9794427).Curated | 1 | |
Sequence conflicti | 600 | A → T in BAA34674 (PubMed:9794427).Curated | 1 | |
Sequence conflicti | 663 | D → G in BAA34674 (PubMed:9794427).Curated | 1 | |
Sequence conflicti | 678 | I → N in BAA34674 (PubMed:9794427).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014636 | 182 – 185 | ALCS → EQSL in isoform 2. 1 Publication | 4 | |
Alternative sequenceiVSP_014637 | 186 – 685 | Missing in isoform 2. 1 PublicationAdd BLAST | 500 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009459 mRNA Translation: BAA34674.1 AJ250369 mRNA Translation: CAB63701.1 Y19160 mRNA Translation: CAB65247.1 Y19163 mRNA Translation: CAB65250.1 AK050052 mRNA Translation: BAC34052.1 AL606969 Genomic DNA No translation available. BC013893 mRNA Translation: AAH13893.1 |
CCDSi | CCDS18941.1 [Q91WP0-1] CCDS18942.1 [Q91WP0-2] |
RefSeqi | NP_001003893.1, NM_001003893.2 [Q91WP0-1] NP_034897.1, NM_010767.3 [Q91WP0-2] |
Genome annotation databases
Ensembli | ENSMUST00000052060; ENSMUSP00000049729; ENSMUSG00000028979 [Q91WP0-1] ENSMUST00000105701; ENSMUSP00000101326; ENSMUSG00000028979 [Q91WP0-2] |
GeneIDi | 17175 |
KEGGi | mmu:17175 |
UCSCi | uc008vux.1, mouse [Q91WP0-2] uc008vuy.1, mouse [Q91WP0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009459 mRNA Translation: BAA34674.1 AJ250369 mRNA Translation: CAB63701.1 Y19160 mRNA Translation: CAB65247.1 Y19163 mRNA Translation: CAB65250.1 AK050052 mRNA Translation: BAC34052.1 AL606969 Genomic DNA No translation available. BC013893 mRNA Translation: AAH13893.1 |
CCDSi | CCDS18941.1 [Q91WP0-1] CCDS18942.1 [Q91WP0-2] |
RefSeqi | NP_001003893.1, NM_001003893.2 [Q91WP0-1] NP_034897.1, NM_010767.3 [Q91WP0-2] |
3D structure databases
SMRi | Q91WP0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201317, 1 interactor |
STRINGi | 10090.ENSMUSP00000049729 |
Protein family/group databases
MEROPSi | S01.229 |
PTM databases
GlyGeni | Q91WP0, 5 sites |
iPTMneti | Q91WP0 |
PhosphoSitePlusi | Q91WP0 |
Proteomic databases
CPTACi | non-CPTAC-3430 non-CPTAC-3722 |
PaxDbi | Q91WP0 |
PeptideAtlasi | Q91WP0 |
PRIDEi | Q91WP0 |
Protocols and materials databases
Antibodypediai | 27998, 337 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000052060; ENSMUSP00000049729; ENSMUSG00000028979 [Q91WP0-1] ENSMUST00000105701; ENSMUSP00000101326; ENSMUSG00000028979 [Q91WP0-2] |
GeneIDi | 17175 |
KEGGi | mmu:17175 |
UCSCi | uc008vux.1, mouse [Q91WP0-2] uc008vuy.1, mouse [Q91WP0-1] |
Organism-specific databases
CTDi | 10747 |
MGIi | MGI:1330832, Masp2 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00950000183084 |
HOGENOMi | CLU_103588_3_0_1 |
InParanoidi | Q91WP0 |
OMAi | QWPKPVF |
OrthoDBi | 156878at2759 |
PhylomeDBi | Q91WP0 |
TreeFami | TF330373 |
Enzyme and pathway databases
BRENDAi | 3.4.21.104, 3474 |
Reactomei | R-MMU-166662, Lectin pathway of complement activation R-MMU-166663, Initial triggering of complement R-MMU-2855086, Ficolins bind to repetitive carbohydrate structures on the target cell surface |
Miscellaneous databases
BioGRID-ORCSi | 17175, 1 hit in 17 CRISPR screens |
ChiTaRSi | Masp2, mouse |
PROi | PR:Q91WP0 |
RNActi | Q91WP0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028979, Expressed in liver and 108 other tissues |
Genevisiblei | Q91WP0, MM |
Family and domain databases
CDDi | cd00033, CCP, 2 hits cd00041, CUB, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR000859, CUB_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR037571, MASP2 IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR035914, Sperma_CUB_dom_sf IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24255:SF10, PTHR24255:SF10, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF07645, EGF_CA, 1 hit PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 2 hits SM00042, CUB, 2 hits SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57535, SSF57535, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01180, CUB, 2 hits PS01186, EGF_2, 1 hit PS01187, EGF_CA, 1 hit PS50923, SUSHI, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MASP2_MOUSE | |
Accessioni | Q91WP0Primary (citable) accession number: Q91WP0 Secondary accession number(s): B1ARY2 Q9Z338 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries