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Protein

Mannan-binding lectin serine protease 2

Gene

Masp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase (By similarity).By similarity

Miscellaneous

Dimerization and MBL2 binding requires calcium ions.By similarity

Catalytic activityi

Selective cleavage after Arg-223 in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg-76 in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Calcium 1By similarity1
Metal bindingi75Calcium 1By similarity1
Metal bindingi120Calcium 1By similarity1
Metal bindingi122Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi123Calcium 1By similarity1
Metal bindingi138Calcium 2By similarity1
Metal bindingi141Calcium 2By similarity1
Metal bindingi158Calcium 2By similarity1
Metal bindingi162Calcium 2; via carbonyl oxygenBy similarity1
Active sitei483Charge relay systemBy similarity1
Active sitei532Charge relay systemBy similarity1
Active sitei632Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.104 3474
ReactomeiR-MMU-166662 Lectin pathway of complement activation
R-MMU-166663 Initial triggering of complement
R-MMU-2855086 Ficolins bind to repetitive carbohydrate structures on the target cell surface

Protein family/group databases

MEROPSiS01.229

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan-binding lectin serine protease 2 (EC:3.4.21.104)
Alternative name(s):
MBL-associated serine protease 2
Mannose-binding protein-associated serine protease 2
Short name:
MASP-2
Cleaved into the following 2 chains:
Gene namesi
Name:Masp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1330832 Masp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000002760120 – 685Mannan-binding lectin serine protease 2Add BLAST666
ChainiPRO_000002760220 – 443Mannan-binding lectin serine protease 2 A chainAdd BLAST424
ChainiPRO_0000027603444 – 685Mannan-binding lectin serine protease 2 B chainAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi72 ↔ 90By similarity
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 156By similarity
Disulfide bondi152 ↔ 165By similarity
Modified residuei158(3R)-3-hydroxyasparagineSequence analysis1
Disulfide bondi167 ↔ 180By similarity
Disulfide bondi184 ↔ 211By similarity
Disulfide bondi241 ↔ 259By similarity
Glycosylationi285N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi300 ↔ 348By similarity
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi328 ↔ 361By similarity
Disulfide bondi366 ↔ 411By similarity
Disulfide bondi396 ↔ 429By similarity
Disulfide bondi433 ↔ 552Interchain (between A and B chains)PROSITE-ProRule annotation
Glycosylationi545N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi598 ↔ 617By similarity
Disulfide bondi628 ↔ 659By similarity
Glycosylationi641N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei443 – 444CleavageBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiQ91WP0
PeptideAtlasiQ91WP0
PRIDEiQ91WP0

PTM databases

iPTMnetiQ91WP0
PhosphoSitePlusiQ91WP0

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiENSMUSG00000028979
CleanExiMM_MASP2
GenevisibleiQ91WP0 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Binds MBL2. Isoform 2 binds to MASP1. Binds SERPING1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049729

Structurei

3D structure databases

ProteinModelPortaliQ91WP0
SMRiQ91WP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 137CUB 1PROSITE-ProRule annotationAdd BLAST118
Domaini138 – 181EGF-like; calcium-bindingAdd BLAST44
Domaini184 – 296CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini298 – 363Sushi 1PROSITE-ProRule annotationAdd BLAST66
Domaini364 – 431Sushi 2PROSITE-ProRule annotationAdd BLAST68
Domaini444 – 683Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118890
HOVERGENiHBG000559
InParanoidiQ91WP0
KOiK03993
OMAiNHLGGFY
OrthoDBiEOG091G02DS
PhylomeDBiQ91WP0
TreeFamiTF330373

Family and domain databases

CDDicd00033 CCP, 2 hits
cd00041 CUB, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR000859 CUB_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR037571 MASP2
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035914 Sperma_CUB_dom_sf
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
PANTHERiPTHR24255:SF10 PTHR24255:SF10, 1 hit
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF07645 EGF_CA, 1 hit
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 2 hits
SM00042 CUB, 2 hits
SM00181 EGF, 1 hit
SM00179 EGF_CA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF50494 SSF50494, 1 hit
SSF57535 SSF57535, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01180 CUB, 2 hits
PS01186 EGF_2, 1 hit
PS01187 EGF_CA, 1 hit
PS50923 SUSHI, 2 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91WP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLLIFLGLL WSLVATLLGS KWPEPVFGRL VSPGFPEKYA DHQDRSWTLT
60 70 80 90 100
APPGYRLRLY FTHFDLELSY RCEYDFVKLS SGTKVLATLC GQESTDTEQA
110 120 130 140 150
PGNDTFYSLG PSLKVTFHSD YSNEKPFTGF EAFYAAEDVD ECRVSLGDSV
160 170 180 190 200
PCDHYCHNYL GGYYCSCRAG YVLHQNKHTC SALCSGQVFT GRSGYLSSPE
210 220 230 240 250
YPQPYPKLSS CTYSIRLEDG FSVILDFVES FDVETHPEAQ CPYDSLKIQT
260 270 280 290 300
DKGEHGPFCG KTLPPRIETD SHKVTITFAT DESGNHTGWK IHYTSTARPC
310 320 330 340 350
PDPTAPPNGS ISPVQAIYVL KDRFSVFCKT GFELLQGSVP LKSFTAVCQK
360 370 380 390 400
DGSWDRPMPE CSIIDCGPPD DLPNGHVDYI TGPEVTTYKA VIQYSCEETF
410 420 430 440 450
YTMSSNGKYV CEADGFWTSS KGEKLPPVCE PVCGLSTHTI GGRIVGGQPA
460 470 480 490 500
KPGDFPWQVL LLGQTTAAAG ALIHDNWVLT AAHAVYEKRM AASSLNIRMG
510 520 530 540 550
ILKRLSPHYT QAWPEEIFIH EGYTHGAGFD NDIALIKLKN KVTINGSIMP
560 570 580 590 600
VCLPRKEAAS LMRTDFTGTV AGWGLTQKGL LARNLMFVDI PIADHQKCTA
610 620 630 640 650
VYEKLYPGVR VSANMLCAGL ETGGKDSCRG DSGGALVFLD NETQRWFVGG
660 670 680
IVSWGSINCG AADQYGVYTK VINYIPWIEN IISNF
Length:685
Mass (Da):75,517
Last modified:December 1, 2001 - v1
Checksum:iF56A6D522BC7099D
GO
Isoform 2 (identifier: Q91WP0-2) [UniParc]FASTAAdd to basket
Also known as: MAp19

The sequence of this isoform differs from the canonical sequence as follows:
     182-185: ALCS → EQSL
     186-685: Missing.

Show »
Length:185
Mass (Da):20,974
Checksum:i72E01900D30A18B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 2MR → MSLPCPQ (PubMed:10586086).Curated2
Sequence conflicti172V → I in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti172V → I in CAB65247 (PubMed:10586086).Curated1
Sequence conflicti192R → K in CAB65250 (PubMed:10586086).Curated1
Sequence conflicti317I → T in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti325S → Y in CAB65250 (PubMed:10586086).Curated1
Sequence conflicti384E → Q in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti600A → T in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti663D → G in BAA34674 (PubMed:9794427).Curated1
Sequence conflicti678I → N in BAA34674 (PubMed:9794427).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014636182 – 185ALCS → EQSL in isoform 2. 1 Publication4
Alternative sequenceiVSP_014637186 – 685Missing in isoform 2. 1 PublicationAdd BLAST500

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009459 mRNA Translation: BAA34674.1
AJ250369 mRNA Translation: CAB63701.1
Y19160 mRNA Translation: CAB65247.1
Y19163 mRNA Translation: CAB65250.1
AK050052 mRNA Translation: BAC34052.1
AL606969 Genomic DNA No translation available.
BC013893 mRNA Translation: AAH13893.1
CCDSiCCDS18941.1 [Q91WP0-1]
CCDS18942.1 [Q91WP0-2]
RefSeqiNP_001003893.1, NM_001003893.2 [Q91WP0-1]
NP_034897.1, NM_010767.3 [Q91WP0-2]
UniGeneiMm.378962

Genome annotation databases

EnsembliENSMUST00000052060; ENSMUSP00000049729; ENSMUSG00000028979 [Q91WP0-1]
ENSMUST00000105701; ENSMUSP00000101326; ENSMUSG00000028979 [Q91WP0-2]
GeneIDi17175
KEGGimmu:17175
UCSCiuc008vux.1 mouse [Q91WP0-2]
uc008vuy.1 mouse [Q91WP0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMASP2_MOUSE
AccessioniPrimary (citable) accession number: Q91WP0
Secondary accession number(s): B1ARY2
, B1ARY3, Q9QXA4, Q9QXD2, Q9QXD5, Q9Z338
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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